• Journal of the Korean Society of Manufacturing Process Engineers
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• v.17 no.6
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• pp.144-150
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• 2018

In this study, the freezing process of L-shaped pipe exposed to the outside was investigated numerically by considering the mushy zone of freezing water. From the numerical results, it was found that the flow was outwardly directed due to the influence of the L-shaped bending part in the outside exposed part of the pipe, and the ice was formed in the shape of longitudinal corrugation on the wall surface of the pipe after the bending part. It is confirmed that this phenomenon is caused by the venturi effect due to the freezing as seen in connection with the velocity distribution in the pipe. It is found that the remelting phenomenon at the end of the freezing section occur simultaneously during the process of forming the ice in the pipe section. In regard of the factors affecting freezing, it was found that the thickness of the freezing layer is increased as the exposed pipe surface temperature is decreased, and the pipe surface temperature had a significant effect on the change of the freezing layer thickness. At the same time, it was found that the freezing layer becomes relatively thin when the water inflow rate is increased. This phenomenon was caused by reducing the exposure time of freezing water due to the vigorous flow convection of the water fluid.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.357-363
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• 2018

The objective of this study was to investigate whether exogenous proline (Pro) could confer freezing tolerance of spinach and determine fluctuations of free amino acids in spinach leaf tissues under freeze-induced stress. Treatment with Pro (10 mM) resulted in more accumulation of Pro (~2.6-fold) in Pro-treated spinaches compared to untreated ones. These Pro-pretreated spinaches were more freezing-tolerant, showing more turgid leaves and petioles compared to untreated controls. However, when spinaches pre-treated with or without Pro were subjected to freezing, there was no significant difference in overall amino acid contents, emphasizing the role of Pro as an osmoprotectant. Freezing stress prompted intensification of total amino acid contents irrespective of pretreatment with Pro. Asp, Glu, Ala, and Val were the most abundant free amino acids due to increased protein degradation and nitrogen mobilization for plant survival under freezing stress. Arg, a precursor for the synthesis of polyamines in plants, was profoundly enhanced under freezing stress. This implies that Arg plays an important role in modulating freezing tolerance. Gly, Leu, and Ile were maintained at relatively low levels in all treatments. However, Ser, Tyr, and Lys as primary constituents of dehydrins were accumulated under freezing stress, suggesting that they might play a role in increasing cryoprotective activity under freezing stress.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1355-1369
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• 2021

This study improved the algorithm for the road ice prediction algorithm and analyzed the prediction rate when comparing actual field measurement data and algorithm prediction value. For analysis, road and weather conditions were measured in Geumdong-ri, Sinbuk-myeon, Pocheon-si. First algorithm selected previous research result algorithm. And the 4th algorithm was improved according to the actual freezing conditions and measured values. Finally, five algorithms were developed: freezing by condensation, freezing by precipitation, freezing by snow, continuous freezing, and freezing by wind speed. When forecasting using an algorithm at the Pocheon site, the freezing hit rate was improved to 93.2%. When calculating the combination ratio for the algorithm. the algorithm for freezing due to condensation and the continuation of the frozen state accounted for 95.7%.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.764-767
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• 1997

This paper presents the modulation of the dexterity of a parallel manipulator using joint freezing/releasing and joint unactuation/actuation. In this paper, individual limbs have redundant number of joints, and each joint can be frozen/released and unactuated/actuated, as needed. First, given a task, the restrictions on joint freezing and joint unactuation of a parallel manipulator are derived. Next, with/without joint freezing and/or joint unactuation, the kinematics of a parallel manipulator is formulated, based on which the manipulability ellipsoid is defined. The effects of joint freezing and joint unactuation on the manipulability are analyzed and compared. Finally, simulation results for a planar parallel manipulator are given. Joint mechanisms, such as joint freezing and joint unactuation, are rather simple to adopt into a parallel manipulator, but is quite effective to improve the task adaptability of the system.

• Journal of Embryo Transfer
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• v.7 no.2
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• pp.81-88
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• 1992

Cryopreservation of mouse embryos biopsied at 4-cell stage was investigated by ultrarapid freezing. Four-cell embryos were obtained from ICR mice on 55h after hCG injection. Zona pellucida of the embryos were partially dissected with a cutting pipet, and then single blastomeres were biopsied from the embryos followed by incubation in $Ca^2$+ and $Mg^2$+-free M16 medium for 30min. Biopsied embryos cultured for lh or 15h were frozen by ultrarapid freezing method using 3M DMSO or 5M glycerol as a cryoprotectant, respectively. The developmental rate of biopsied embryos after ultrarapid freezing and thawing to blastocysts was 81 % in the group of biopsied embryos cultured for lb and 98% in the group of biopsied embryos cultured for 15h, respectively. When biopsied embryos after ultrarapid freezing and thawing were transferred to the uteri of pseudopregnant recipients, normal live young were born. These results suggest that this freezing method can efficiently cryopreserve biopsied mouse embryos.

• Journal of Advanced Marine Engineering and Technology
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• v.25 no.6
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• pp.1250-1259
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• 2001

This study was experimentally performed to investigate sea water freezing behavior along parallel cooled plate with bubbly flow The experiments were carried out for a variety of parameter, such as sea water velocity, air-bubble flow rate, and cooled-plate temperature. The shape of freezing layer, freezing rate and salinity of frozen layer were observed and measured. It was found that the experimental parameters gave a great influence on the freezing rate and the salinity of the frozen layer.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.3 no.5
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• pp.386-394
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• 1991

Freezing of saturated porous media contained in a rectangular cavity has been studied experimentally. Water and different diameter glass beads consitituted the liquid and porous media. Solidification front shape, the effects of bead diameter and initial liquid temperature was investigated. When the hot wall temperature was below $4^{\circ}C$, the freezing rate was higher at the top than at the bottom due to the density inversion, but with increasing the hot wall temperature the freezing rate at the top was effected by the liquid temperature and was lower than at the bottom. With increasing the bead diameter, the difference of freezing rate between top and bottom was increased and depends on thermal conductivity. When the liquid temperature was low in the beginning, the freezing rate was high, but with increasing the time almost the same with those of high temperature liquid.

• Journal of The Korean Society of Agricultural Engineers
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• v.46 no.2
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• pp.59-66
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• 2004

This study was performed to evaluate the freezing and thawing properties of the high strength concrete using recycled coarse aggregate. The recycled coarse aggregate replaced natural crushed aggregate by 0%, 25%, 50%, 75% and 100%. The compressive strength of the concrete using recycled coarse aggregate showed more than 300 kgf/$cm^2$ at the curing age 28 days. The mass loss ratio by freezing and thawing was less than 1% at all mix type. The relative dynamic modulus of elasticity was decreased with increasing the freezing and thawing cycles. Also, the durability factor by the freezing and thawing was decreased with increasing the content of recycled coarse aggregate. But, the recycled concrete except 100% recycled coarse aggregate showed 60 or more durability factor in the freezing and thawing 300 cycles. Accordingly, these recycled coarse aggregate can be used for high strength concrete.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.420-430
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• 2000

These studies were preformed to investigate the freezing conditions to achieve good post-thaw viability of spend and the practical methods of artificial insemination frozen canine semen. Semen were collected from nine male dogs which had been proved to be fertile in the past and the semen were treated for freezing procedure. Post-thaw motility and viability of canine sperm were evaluated to investigate individual tolerance of freezing, difference among freezing extenders, dif-ference among freezing equipments and freezing conditions, difference between fast and slow cooling rate, difference according to different glycerol concentration, effect of seeding on post-thaw viability, difference according to cutting part of straw, difference according to thawing temperatures, and dif-ference according to media added to thawed semen. Thawed semen for insemination were added with equal volnme of canine capacitation medium (CCM) and the volume of semen and the number per insemination were adjusted as 2-3 ml and $20-30 {\times}10^7,$ respectively. The semen were inseminated in vagina using balloon catheter and en17ryos were cellected from 9 to 11 days after the second Al to d determine fertilization.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.8 no.3
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• pp.413-422
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• 1996

Freezing of water in von-$K{\acute{a}}rm{\acute{a}}n$ swirling flow is considered. The transient behavior of the temperature distribution in both solid and liquid phases and freezing rate are determined. The fluid flow induced by the rotation of solid strongly inhibits the freezing process. The thickness of frozen layer is inversely proportional to the square root of the angular velocity of solid. As the angular velocity or initial liquid temperature becomes larger, the freezing process is more strongly inhibited by the fluid flow. When phase change is present, the transient heat transfer rate is greater than the case with no phase change.