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Changes of Activities of Oxygen Free Radical, Generating and Scavenging Enzymes in Rat Liver Induced by Scald Burn Injury (피부화상에 의한 간 조직의 oxygen free radical 생성계 효소와 해독계 효소의 활성변화)

  • Kim Han-Soo;Cho Hyun-Gug;Kim Sang-Soo;Bae Ju-Han;Seo Hyun-Kyu
    • The Journal of Korean Physical Therapy
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    • v.11 no.3
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    • pp.115-121
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    • 1999
  • The effects of scald bum Injury ($40-45\%$ of total body surface area), there were not inhalation and secondary infection, on the histological changes and the levels of oxygen free radical generating and scavenging enzymes have been determined in liver tissue of rat models. It was found that dermal epithelium was left out with edema of dermis layer and hydorpic swelling of hepatocytes, Burn injury increased liver weight (L.W./B.W.) and serum aspartate aminotransferase content (pThe data of this study suggest that liver damage Induced by scald burn injury leads to dysbalance of oxygen free radical generating and scavenging enzymes.

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Electrolyte Effect on the Particle Characteristics Prepared by Soap-Free Emulsion Polymerization

  • Han, Seung-Tak;Lee, Kang-Seok;Shim, Sang-Eun;Saikia, Prakash J.;Choe, Soon-Ja;Cheong, In-Woo
    • Macromolecular Research
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    • v.15 no.5
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    • pp.403-411
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    • 2007
  • Monodisperse micron-sized polystyrene (PS) spheres were successfully obtained using a single stage soap-free emulsion method in aqueous media mixed with ethanol (co-solvent) containing NaCI as the electrolyte. The optimum conditions for preparing the monodisperse PS microspheres, using soap-free emulsion polymerization in a water/ethanol mixture with an electrolyte, were studied. The presence of the co-solvent and electrolyte controlled the particle dispersion stability during the polymerization. The microspheres formed using PS, with a weight-average diameter of $2.6{\mu}m$, coefficient of variation of 5.3% and zeta potential of -15.1 eV, were successfully obtained in the presence of 0.1 wt% NaCI, 10 wt% monomer, 0.1 wt% initiator and 95/5 (g/g) of a water/ethanol mixture reacted at $70^{\circ}C$ for 24 h.

Changes in $\alpha$-Amylase Activity and Free Sugar Contents of Buckwheat during Germination (메밀 발아 중 $\alpha$-Amylase 활성도와 유리당 함량의 변화)

  • 이명헌;손흥수
    • The Korean Journal of Food And Nutrition
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    • v.8 no.1
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    • pp.32-36
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    • 1995
  • Changes in the w-amylase activity and free sugar contents were Investigated during buckwheat (Fagopyrum esculentum Moench) germination at 1$0^{\circ}C$ for 7 days. The a-amylase activity in ungerminated seeds was 1.66 U. It increased for the 1st day of germination, but then decreased until 3rd day, and thereafter Increased. The free sugar contents In ungerminated seeds were as follows. The maltose, fructose, glucose and rhamnose were 1.81mg%, 0.42mg%, 7.71mg%, 6.80mg% on dry weight basis, respectively. The maltose and fructose contents decreased in the initial stage of germination, but then gradually increased. The glucose contents decreased for the 3rd day, but sharply Increased afterwords. The rhamnose contents decreased until 1 day, and then there was no significant change for the 6 days.

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Effect of Growth Regulator, Sucrose, and Minimal-growth Conservation on In Vitro Propagation of Virus-free Sweet Potato Plantlets (고구마 무병묘의 기내 증식에 미치는 생장조절물질, Sucrose, 최소생장 보존의 영향)

  • Lee, Na Rha;Lee, Seung Yeob
    • Journal of Bio-Environment Control
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    • v.29 no.1
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    • pp.1-8
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    • 2020
  • The influence of growth regulators (NAA and BA) and sucrose concentrations (0, 3, 5, 7, 9%) on in vitro rapid-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] was investigated with single-node or shoot-tip culture of two cultivars ('Matnami' and 'Shinhwangmi'). The survival rate and growth of shoot-tip explant was also investigated under the presence or absence of light (blue and red LED = 7:3, 150±5 μmol·m-2·s-1 PPFD) during minimal-growth in vitro conservation at 15℃. Vine length, vine diameter, fresh weight and dry weight were enhanced without callusing of explant in the MS medium supplemented with 0.2-0.5 mg·L-1 BA. The growth of single-node and shoot-tip explants were significantly enhanced with the increase of vine length, number of leaf, number of root, fresh weight, and dry weight in the solid medium containing 5% sucrose and 0.2 mg·L-1 BA. Vine elongation of shoot-tip explants were highest in the liquid medium containing 3% sucrose than the solid medium. The survival rate of minimal-growth in vitro conservation was 100% in 5 months under the presence of light (LED, 150±5 μmol·m-2·s-1 PPFD) at 15℃, but the explants in dark condition died in 3 months. The light was absolutely necessary for the in vitro conservation under minimal-growth conditions of virus-free sweet potato plantlets at 15℃, and the high density of explants (10 plantlets per Petri Dish) was increased the efficiency of mass conservation.

Anti-obesity Effects of SBY-III in High Fat Diet-Fed Obese Rats Continued by High Fat Diet and Regulated by Normal Diet (SBY-III이 비만 및 비만 후 식이조절 흰쥐에 미치는 영향)

  • Woo, Kyung-Ha;Chung, Seok-Hee;Lee, Jong-Su;Kim, Sung-Soo;Shin, Hyun-Dae
    • Journal of Korean Medicine Rehabilitation
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    • v.15 no.2
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    • pp.117-117
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    • 2005
  • Objectives : This experimental study was designed to investigate the effect of SBY-III extract on the weight, cell size of epididymal fat-pad, fat accumulation area in liver, serum lipid level and UCP1 mRNA in brown adipose tissue of high fat diet-fed obese rats continued by high fat diet and regulated by normal Diet. Methods : The body weight gain, weight of the internal organs(epididymis, liver, brown adipose tissue), insulin, triglyceride, total cholesterol, total lopod, free fatty acid, expression of UCP1 mRNA were measured in high fat diet-fed obese rats continued by high fat diet and regulated by normal diet. The experimental study are divided into exp-I and exp-II. Each study was administered normal diet, high fat diet and SBY-III according to each situation. Normal group is normal diet for 8 weeks. Exp-I are divided into control group(high fat diet for 8 weeks) and sample group(high fat diet for 8 weeks and SBY-III for last 2 weeks). Exp-II are divided into control group(high fat diet for 6 weeks and normal diet for 2 weeks) and sample group(high fat diet for 6 weeks and normal diet with SBY-III for 2 weeks). These were then compared mutually. Results : 1. Irrespective of diet control, sample group taken SBY-III showed the more effective decrease of weight gain than control group and diet control-fed sample group with SBY-III showed the more effective decrease of weight loss including weight gain than control group. 2. Irrespective of diet control, sample group taken SBY-III showed the more effective decrease cell size of epididymal fat-pad, fat accumulation area in liver than control group. 3. Non diet control-fed sample group taken SBY-III showed the more effective decrease of serum triglyceride, total lipid, free fatty acid than control group and diet control-fed sample group taken SBY-III showed the decrease of serum triglyceride, free fatty acid than control group. 4. Only diet control-fed sample group taken SBY-III showed the decrease of UCP1 volume. Conclusions : These results shows that SBY-III has effects on anti-obesity, especially keeping pace with diet control.

Crank Angles Design to Reduce the Excitation Forces in 4-stroke Diesel Engine (4행정 기관의 기진력 감소를 위한 크랭크 각 설계)

  • 서영수;박정근;정의봉
    • Proceedings of the Korean Society of Marine Engineers Conference
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    • 2000.11a
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    • pp.108-114
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    • 2000
  • The excitation forces from the periodical firing pressure in cylinder and the rotating crank mechanism cause lots of vibration problems in diesel engine. In this paper, the theoretical formulas for excitation forces are introduced and computational program for the optimization of crank angle is also developed to reduce the free moments in diesel engine. The computational results of 4-stroke in-line engine are applied to verify the reliability of the program.

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Ghost-free High Dynamic Range Imaging Based on Brightness Bitmap and Hue-angle Constancy (밝기 비트맵과 색도 일관성을 이용한 무 잔상 High Dynamic Range 영상 생성)

  • Yuan, Xi;Ha, Ho-Gun;Lee, Cheol-Hee;Ha, Yeong-Ho
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.1
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    • pp.111-120
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    • 2015
  • HDR(High dynamic range) imaging is a technique to represent a dynamic range of real world. Exposure fusion is a method to obtain a pseudo-HDR image and it directly fuses multi-exposure images instead of generating the true-HDR image. However, it results ghost artifacts while fusing the multi-exposure images with moving objects. To solve this drawback, temporal consistency assessment is proposed to remove moving objects. Firstly, multi-level threshold bitmap and brightness bitmap are proposed. In addition, hue-angle constancy map between multi-exposure images is proposed for compensating a bitmap. Then, two bitmaps are combined as a temporal weight map. Spatial domain image quality assessment is used to generate a spatial weight map. Finally, two weight maps are applied at each multi-exposure image and combined to get the pseudo-HDR image. In experiments, the proposed method reduces ghost artifacts more than previous methods. The quantitative ghost-free evaluation of the proposed method is also less than others.

Effects of herbal acupuncture (Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) in obese Rats induced by high fat diet (마황(麻黃), 마황합록다(麻黃合綠茶) 약침(藥鍼)이 고지혈증(高脂血症) 유발(誘發) 백서(白鼠)에 미치는 영향(影響))

  • Youn, Dae-Hwan;Kang, Ja-Don;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.21 no.1
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    • pp.51-60
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    • 2004
  • Objectives : This research was performed to investigate the effect of herbal acupuncture(Ephedra sinica and Green tea) at Umnungchon(SP9) and Pungnyung(ST40) on weight gain, food intakes, food efficiency ratios, HDL-cholesterol, triglyceride, free fatty acid on rats fed high fat diet for 5weeks. Methods : Experimental groups were divided into normal group(Sham), high fat diet group(Con), high fat diet and Ephedra sinica-herbal acupuncture in SP9 ST40 treated group(ES), high fat diet and Green tea-herbal acupuncture in SP9 ST40 treated group (GT), high fat diet and Ephedra sinica+Green tea-herbal acupuncture in SP9 ST40 treated group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 19.125mg/ml per 2 days during 5 weeks. Results : Body weight, feed intake and feed efficiency ratio were decreased in Sham, ES, ES+GT, compared with control group. The level of serum HDL-cholesterol was increased in sham, tender to increase in ES, ES+GT. The change on that of serum triglyceride was decreased in sham, ES, ES+GT and free fatty acid was decrease in sham, ES+GT. Conclusions : Ephedra sinica with Green tea-herbal acupuncture in SP9 ST40 can control the Body weight, feed intake and feed efficiency ratio, HDL-cholesterol, triglyceride, free fatty acid.

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Effect of Bunsimgieumgagambang on the Stress Due to the Maternal Separation in Rats (분심기음가감방(分心氣飮加減方)이 모성분리(母性分離) stress 백서(白鼠)에 미치는 영향)

  • Kim Ki-Bong;Kim Jang-Hyun;Chang Gyu-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.5
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    • pp.1303-1310
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    • 2005
  • This study was performed to investigate the effect of Bunsimgieumgagam on the stress due to the maternal separation in rat. In this study, we researched in 'the behavioral observation', 'the changes of body weight', 'quantitative analysis of the number of BrdU-positive cells per section in dentate gyrus of hippocampus', 'free radical scavenging assay' and 'MTT-based cytotoxicity assay of SK-N-SH cell line', in order to figure out the effect on which Bunsimgieumgagam has the increase of neuron in dentate gyrus of hippocampus damaged by the stress due to the maternal separation. In the behavioral Observation, Bunsimgieumgagam was also efficacious against the decline of one's behavior and anorexia derived from the stress by the maternal separation. In the change of body weight, it showed that the Bunsimgieumgagam is effective in the recovery of weight loss caused by heavy stress(p<0.05). Also, Bunsimgieumgagam had an increasing effect, which is similar to a normal state, on DG's neuron in hippocampus (P<0.001). In free radical scavenging assay, Bunsimgieumgagam had a superior free radical scavenging effect. And it showed a significant result with the high cell proliferation effect in MTT-based cytotoxicity assay(P<0.01, p<0.001) This result suggest that Bunsimgieumgagam has an anti-stress effect and a proliferation effect of neuron in dentate gyrus of hippocampus, and it shows the potential of Bunsimgieumgagam in the treatment for the various disorders derived from children's stress.

Experimental Effects of Ijin-tang add Atractylodis Rhizoma and Atratcylodis Macrocephalae Rhizoma Extract in Obese Mice Induced by High Fat Diet (이진탕가창출백출(二陳湯加蒼朮白朮)이 고지방 식이로 유발된 생쥐의 비만증에 미치는 영향)

  • Lee, Sang-Young;Jeong, Hyun-Woo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.1
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    • pp.89-95
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    • 2008
  • This experimental study was designed to investigate the effects of Ijin-tang add Atractylodis rhizoma and Atratcylodis macrocephalae rhizoma (IJTAA) on the change of weight and serum total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, free fatty acid, total lipid and phospholipid level in obese mice induced by high fat diet. Experimental groups were as follows ; Normal group was fed normal diet and administered distilled water during 7 weeks, Control group was fed high fat diet and administered distilled water during 7 weeks, Sample A group was fed high fat diet and administered IJTAA 500 ㎎/㎏/day/mouse during 7 weeks, Sample B group was fed high fat diet and administered IJTAA 700 ㎎/㎏/day/mouse during 7 weeks. The results were as follows ; 1. In Sample A group and Sample B group, There were significantly decreased in body weight, serum total cholesterol level, serum triglyceride level, serum free fatty acid level, serum total lipid level and serum phospholipid level in comparison with Control group. 2. In Sample A group and Sample B group, There were significantly increased in serum HDL-cholesterol level in comparison with Control group. 3. In Sample A group and Sample B group, There were decreased in serum LDL-cholesterol level in comparison with Control group. According to above results, I suggest IJTAA is able to be used for managing obesity by controllong body weight, serum total cholesterol level, serum triglyceride level, serum free fatty acid level, serum total lipid level and serum phospholipid level.