• 한국식품영양학회지
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• 제28권3호
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• pp.499-506
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• 2015

The aim of this study was to investigate the changes in the taste components of cheonggukjang made with germinated soybeans, which is produced by the addition of a smoking process. The total organic acid content of smoked cheonggukjang made with germinated soybeans (SCGS) was four times higher than that of non-smoked cheonggukjang made with non-germinated soybeans (NCNS). The total organic acid content of SCGS was lower than that of non-smoked cheonggukjang made with germinated soybeans (NCGS). The total free sugar content of NCGS and SCGS was approximately three times higher than that of NCNS. The total free amino acid content of NCGS was significantly decreased compared with that of NCNS, whereas the total free amino acid content of SCGS was 1.2 times higher than that of NCGS. The essential amino acid content of SCGS was significantly increased compared with that of NCGS. The ratio of glutamic acid to the total free amino acid in NCNS and SCGS was 19.6%. Similarly, the ratio of glutamic acid to the total free amino acid in NCGS was 19.1%. There were no significant differences in the total fatty acid content of NCNS, NCGS, and SCGS. The sensory evaluation of appearance, aroma, taste, and overall quality of SCGS was significantly increased compared with that of NCNS and NCGS. These results suggest that smoked cheonggukjang made with germinated soybeans could be utilized for the development of a novel fermented food.

• Applied Biological Chemistry
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• 제25권4호
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• pp.239-247
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• 1982

은기(銀寄), 녹조(鹿鳥), 상피(霜被)의 3가지 밤 품종(品種)을 시료(試料)로 하여 과육(果肉)을 내과육(內果肉)과 외과육(外果肉)으로 나눈 후에 유리지질(遊離脂質) 및 결합지질(結合脂質)을 추출(抽出) 하고 그의 지질조성(脂質組成) 및 구성지방산(構成脂肪酸)을 column chromatography, gas chromatography에 의하여 분리(分離), 정량(定量)하였다. 밤의 총지질(總脂質)의 평균함량(고형물당(固刑物當))은 내과육(內果肉)에 1.84%, 외과육(外果肉)에 1.95%였으며 이 중에서 유리지질(遊離脂質)은 내과육(內果肉)에 1.26%, 외과육(外果肉)에 1.63%이나 결합지질(結合脂質)은 내과육(內果肉)에 0.58%, 외과육(外果肉)에 0.32%로서 유리지질이 결합 지질보다 $2{\sim}5$배 많았으며 외과육(外果肉)에 특히 유리지질이 많았다. 유리지질(遊離脂質) 중의 중성지질(中性脂質)은 평균 39.5%로 결합지질(結合脂質)의 4%보다 10배 정도 많았으나 외과육(外果肉)에 있어서는 유리지질(遊離脂質) 중의 중성지질이 56.6%로서 더욱 많았다. 당지질(糖脂質)의 평균함량은 유리지질에 22.0%, 결합지질에 17.0%였으며 인지질(燐脂質)의 평균함량은 유리지질에는 6.9%였으나 결합지질에는 10.5%로써 그 함량(含量)이 많았다. 밤의 유리지질(遊離脂質)을 구성하는 주요 지방산은 linoleic acid, palmitic acid 및 linolenic acid이나 결합지질(結合脂質)에는 palmitic acid가 40.1%로 가장 많았고 linoleic acid, linolenic acid가 그 다음으로 적었다. 또한 결합지질에는 포화지방산(飽和脂肪酸)이 유리지질에서 보다 2배 정도 많았다.

• 동아시아식생활학회지
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• 제25권2호
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• pp.326-332
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• 2015

Makjang is a kind of Korean traditional short-term fermented soybean paste. The purpose of this study was to evaluate the compositions of free sugars, organic acids and free amino acids as well as microbial counts of six kinds of Makjang. Commercial Makjang from different regions (Kangwon-do, Choongchung-do and Kyungsang-do) were used. The major free sugars of samples were glucose (0.15~3.97%) and maltose (0.01~0.54%), whereas sucrose (0.02%) was detected only in M4 and fructose only in M1 and M2. The major organic acids were citric acid (0.16~3.09%), malic acid (0.06~0.23%) and succinic acid (0.01~0.11%). The total content of free amino acids ranged from 0.02 to 8.74 mg/g, and major amino acids were detected in the order of glutamic acid, leucine, alanine and aspartic acid. Numbers of viable cells of bacteria as well as yeast and molds were $7.8{\times}10^4{\sim}4.8{\times}10^5$ and $8.1{\times}10^3{\sim}3.6{\times}10^4$, respectively. The results of the sensory evaluation showed that over-all preference of Kyungsang-do Makjang was higher than others. Consequently these results provide better information for standardizing and improving quality and functional activity of commercial Makjang.

• 한국식품저장유통학회지
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• 제6권4호
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• pp.495-499
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• 1999

민들레 잎과 뿌리의 유리당은 sucrose, glucose, fructose 3종이 분리되었으며, 총 유리당의 함량은 잎에 비해 뿌리에서 높았다. 잎의 유기산 함량은 oxalic acid, citric acid, malic acid이 각각 45.4, 3.6, 2.7mg/100g-f.w.이었고, 뿌리의 경우에는 각각 34.6, 2.1, 1.6mg/100g-f.w.이었다. 총 유기산의 함량은 잎과 뿌리에서 각각 51.7, 38.3mg/100g-f.w.이었는데 뿌리에 비해 잎에서 그 함량이 높았다. 민들레의 잎과 뿌리의 유리아미노산 중에 aspartic acid, serine, asparagine, glutamic acid, glycine, valine, isoleucine의 함량이 높았고, 특히glutamic acid의 함량이 높았다. 민들레의 비타민 A 함량은 잎과 뿌리에서 각각 135.4, 34.1$\mu\textrm{g}$/100g-f.w.이었고, 비타민 C의 함량은 각각 67.4, 4.6mg/100g-f.w.이었다.

• 한국동물학회지
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• 제4권2호
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• pp.13-19
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• 1961

Free amino acids at five different developemntal stages (Gastrulation-Hatching -out stage) of Hynobius leechi BOULENGER were analyzed qualitatively by the use of paper paitition chromatography. It was found that the number of free amino acids increased as the development proceeded. The free amino acids identified at each stages are as follows : Gastrulation stage : Alaninie, Aspartic acid, Glutamin acid, Histidine, Methionine. Neural plate formation stage : Alanine , Aspartic acid, Glutamic acid, Glycine, Histidine, MEthionine, Phenylalanine, Proline, Serine, Trypotophan. Middle tail-bud stage : Alanine, Arginine, Asparagine,Aspartic acid, Citrulline, Glutamic acid, Glycine, Histidie,Hydroxyproline, Proline, Leucine, Methionine, Ornithine, Phenylalanine, Serine, Threonine, Tryptophan. Late tail-bud stage : Alanine, Arginine, Asparagine, Aspartic acid, Citrulline, Glutamic acid. Glycine, Histidine, Hydroxyproline, Leucine, Methionine, Ornithine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Valine. Hatching -out stage : the same with the late tail-bud stage. It seems probable that the metabolic systems of amino acids before and after the middle tail-bud stage are quite different from each other. Before the middle tail=-bud stage, the reaction system of amino acids is thought not to be completed while after that stage the system has been completed , because in the former period of the development , the number of freeamino acids increased rapidly with the development , and after that stage, there is practically no change in the number of free amino acids.

• 한국식품영양과학회지
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• 제27권1호
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• pp.10-15
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• 1998

In order to establish the quality standard of commercial soybean paste, pH, color, moisture, crude protein, isoflavone, free amino acid and organic acid contents of the fermented soybean food in both traditional and commercial products were analyzed. The crude protein contents were from 5.0 to 11.9% in commercial soybean pasted of Samjang and traditional soybean paste of Chungugjang, and the latter showed higher value than those of the former. The contents of isoflavones in commercial soybean paste were less than those of traditional soybean paste. Total contents of free amino acids were distributed from 301 to 4,602mg%, respectively, in which Chungugjang showed the smallest value of 301mg% and the traditional soybean paste showed the most abundant one of 4,602mg%. The ratio of glutamic acid to total free amino acids were 15.9% and 30.1% in traditional soybean paste and commercial soybean paste, respectively. The major organic acid both commercial soybean paste and Samjang was citric acid containing 109.9~196.5mg%. On the other hand, that of traditional soybean paste and Chungugjang was oxalic acid containing 82.8~130.1mg%. This results might be caused by the differences of brewed periods, microflora, processing methods and used raw materials.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1068-1076
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• 1997

This study was carried out to elucidate the utility of fusant for shortening the ripening time of imitation processed cheese. L. bulgaricus exhibited the highest protease and lactase activity and L. helveticus revealed the highest lipase activity. Fusant was shown to be high in the activity of protease and lactase. The total volatile free fatty acid produced by the cheese treated with L. helveticus was markedly increased after four ripening days and was gently increased after nine ripening days. However, L. bulgaricus significantly increased the total volatile free fatty acid between four and nine ripening days. In the case of fusant, the amount of total volatile free fatty acid was observed to increase at a constant rate relative to the ripening time. In free fatty acid production at different ripening times, L. bulfaricus generated caproic acid and caprilic acid abundantly while it produced a poor quantity of capric acid, lauric acid, and myristic acid. In the cheese sample treated with L. helveticus, the amount of caproic acid and capylic acid was on increase as the ripening time increased. The amount of caproic acid and caprylic acid produced by fusant was less than that produced by the other two starters. In the panel sensory evaluation, the flavor intensity and preference increased as the ripening time increased. The cheese sample treated with fusant showed the highest flavor intensity at 7 days, whereas cheese treated with L. helveticus exhibited the highest flavor intensity at 15 or 30 days. The cheese treated with L. helveticus showed the highest preference at 7 days, but cheese treated with fusant exhibited the highest preference at 30 days.

• 한국식품조리과학회지
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• 제23권1호통권97호
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• pp.150-155
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• 2007

This work was conducted to obtain a rapid, accurate, and precise procedure for free amino acids analysis in Doenjang with HPLC-OPA (high performance liquid chromatography using-phthalaldehyde) and AAA (automatic amino acid analyzer) methods. Different sample extraction procedures among water, 0.1 M perchloric acid, and 0.1% meta-phosphoric acid were also compared. The optimal extraction solvent was 0.1% meta-phosphoric acid for both the HPLC-OPA and AAA methods. Good recoveries for glycine and methionine were observed using the 0.1% meta-phosphoric acid extraction with HPLC-OPA method. Method precisions (% relative standard deviation) for the free amino acids ranged for 1.62% to 8.27%, in which the HPLC-OPA method with water extraction showed the lowest value at 1.62%. Inhibition rates of the free amino acids in Doenjang were greatest with an addition of NaCI at a 1% concentration.

• Bulletin of the Korean Chemical Society
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• 제20권1호
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• pp.59-64
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• 1999

Based on the study of hydrolysis behaviour of U(Ⅵ) ion and major fission product metal ions such as Cs(Ⅰ), Ce(Ⅲ), Nd(Ⅲ), Mo(Ⅵ), Ru(Ⅱ), and ZR(Ⅳ) in the titration media, the performance of noncomplexing-alkalimetric titration method for the determination of free acid in the presence of these metal ions was investigated and its results were compared to those from the completing methods. The free acidities could be determined as low as 0.05 meq in uranium solutions in which the molar ratio of U(Ⅵ)/H+ was less than 5, when the end-point of titration was estimated by Gran plot. The biases in the determinations were less than 1% and about +3% respectively for 0.4 meq and 0.05 meq of free acid at the U(Vl)/H+ molar ratio of up to 5. Applicability of this method to the determination of free acid in spent fuel solutions was confirmed by the analysis of nitric acid content in simulated spent fuel solutions and in a real spent fuel solution.

• 대한가정학회지
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• 제23권1호
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• pp.25-31
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• 1985

This study designed not only to elucidate free amino acid, fatty acid composition and mineral content in dried alaska pollack but also to examine calcium, magnesium, poassium and sodium contents in its cooking broth according to each boiling time such as 2.5, 5, 10, 20, and 30 minutes. RESULTS : 1. Glutamic acid in dried alaska pollack was the most abundant free amino acid and was followed in order of aspartic acid, lysin, leucine and histidine. They composed of 50.95% of the total amino acid contents. 2. In dried alaska pollack the most abundant fatty acid was palmitic acid. Essential fatty acid content was 26.57% of the total fatty acid. 3. The calcium, magnesium, potassium and sodium contents in dried alaska pollack showed 10.721 mg%, 9.235mg%, 4072.5mg% and 2299.8% respectively. The amount of magnesium and potassium in cooking broth arrived at the highest level when 20 minute boiling was undertaken and the quantities of magnesium and sodium were the highest when 30 minute boiling was made.