• Title/Summary/Keyword: fractionation.

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Mass Spectrometry-Based Proteomic Profiling of Pseudopodia of Metastatic Cancer Cells

  • Choi, Sunkyu
    • Mass Spectrometry Letters
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    • v.11 no.2
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    • pp.25-29
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    • 2020
  • Pseudopodia are dynamic actin cytoskeleton-based membrane protrusions of cells that enable directional cell migration. Pseudopodia of cancer cells play key roles in cancer metastasis. Recent studies using pseudopodial subcellular fractionation methodologies combined with mass spectrometry-based proteomic profiling have provided insight into the pseudopodiome that control the protrusions of invasive metastatic cancer cells. This review highlights how to characterize the protein composition of pseudopodia and develop strategies to identify biomarkers or drug candidates that target reduction or prevention of metastatic cancer.

Separations of polystyrene sulfonate by dual pump field flow fractionation using UF hollow fiber membranes (듀얼펌프 중공사막 흐름 장 흐름 분회법을 사용하여 다당류(Polystyrene sulfonate)의 분리)

  • 정도연;최승렬;이승윤;민병렬
    • Proceedings of the Membrane Society of Korea Conference
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    • 2004.05b
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    • pp.192-194
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    • 2004
  • FFF에서 외부장은 시료의 종류에 따라 선택적으로 사용되며, 그 외부장의 종류에 따라 세부기술로 구분되어진다. 원심력(gravitational or centrifugal field)을 외부장으로 사용하는 침강장-흐름분획법(sedimentation FFF)은 지름이 0,05∼1um정도인 콜로이드 입자의 분리에 좋은 방법이다. 온도구배(thermal fradient)를 이용하는 열장-흐름분획법(thermal FFF)은 유기 고분자의 분리 및 고분자의 물성연구에 이용된다.(중략)

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A MECHANISM OF THE STRANGE ISOTOPIC FRACTIONATION OF OXYGEN FOUND IN METEORITE AND LABORATORY

  • Yang, J.;Kim, S.K.;Soh, K.S.;Yee, J.H.;Kim, S.W.;Kim, K.H.;Yoo, K.W.
    • Journal of Astronomy and Space Sciences
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    • v.7 no.1
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    • pp.1-10
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    • 1990
  • The thermodynamic distribution of the vibrational states of ozone coupled with anharmonic predissociation produces an unusual isotopomeric pattern of oxygen molecules. The model presented here explains the experimental data obtained from the electric discharge of oxygen gas to produce ozone condensed on a quartz at 77K.

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Antioxidant Polyphenol Glycosides from the Plant Draba nemorosa

  • Rahman, Md. Aziz Abdur;Moon, Surk-Sik
    • Bulletin of the Korean Chemical Society
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    • v.28 no.5
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    • pp.827-831
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    • 2007
  • Bioassay-directed fractionation of Draba nemorosa led to the isolation of two new phenolic glycosides, 1-O- (sinapoyl)-glucitol (2) and 1,3-disinapoylgentiobiose (5) along with five known phenolic glycosides (1, 3, 4, 6, and 7). Their structures were characterized based on spectroscopic methods (2D NMR, HRTOFMS, IR, and UV). The isolated compounds showed antioxidant activities (IC50) in the range of 14-98 mM which were estimated by DPPH radical-scavenging assay.

Isolation of Antimicrobial Substance from the Korean Traditional Leaf Mustard, Brassica juncea Coss.

  • Kang Seong-Koo
    • Plant Resources
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    • v.8 no.2
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    • pp.145-154
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    • 2005
  • The antimicrobial effect of each fraction after fractionation of an ethanol extract of leaf mustard was examined in terms of nucleic acid, chloroform, ethylacetate, and butanol. The ethylacetate fraction, which showed the strongest level of antimicrobial effect among the different ethanol extract fractions of leaf mustard, was isolated and purified using silica gel column chromatography and HPLC, respectively, to obtain a single antimicrobial substance called KLM-1. The antimicrobial effect of this substance was 10 times higher than that of the ethylacetate fraction. A further study is on the way to confirm the structure of the antimicrobial substance KLM-1 through LC/Mass and NMR.

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Isolation of a Cytotoxic Agent from Asiasari Radix

  • Park, Jong-Dae;Baek, Nam-In;Lee, You-Hui;Kim, Shin-Il
    • Archives of Pharmacal Research
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    • v.19 no.6
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    • pp.559-561
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    • 1996
  • A minor cytotoxic compound was isolated by bioassay-guided fractionation from Asiasari Radix and identified as aristolactam III(1) on the basis of spectral data and chemical evidence. This is the first report on the isolation of compound 1 from Asiasarum genus. Compound 1 exhibited a significant cytotoxic activity against the three kinds of human cancer cell lines (A 549, SK-MEL-2 and SK-OV-3).

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토양 유기물과 산화철의 흡착 반응 및 특성

  • 고일원;김주용;김광구;김경웅
    • Proceedings of the KSEEG Conference
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    • 2003.04a
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    • pp.69-71
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    • 2003
  • 토양 및 수질 유기물질과 금속 산화물사이의 상호 반응관계는 환경지구화학적으로 중요하게 다루어진다 (Sposito, 1984). 토양내 광물들의 표면은 토양 유기물질의 주요한 부분을 차지하는 휴믹물질, 즉 휴믹산과 펄빅산에 의해서 이온강도와 산도에 따라 피복하는 현상을 일으킨다. 특히, 휴믹산과 펄빅산은 토양 및 수질내 주요한 구성 유기물로 존재하며 다양한 유기화합물과 분자량을 갖으며, 중금속들의 유출에 의한 오염 환경에서 그 화학종과 이동도에 영향을 주게된다 (Thurman, 1985). (중략)

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Investigation of Brassinosteroid substances in the shoots of hybrid type korean rices (통일계 벼의 Brassinosteroid활성물질 검색)

  • 현규환;박근형
    • KSBB Journal
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    • v.6 no.2
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    • pp.123-128
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    • 1991
  • The production of bassinosteroid-like substances of two hybrid types of Korean rice, Jangseongbyeo, Taebackbyeo were investigated. The shoots at the maximum tillering stage were extracted and purified by solvent fractionation, silica gel adsorption chromatography Sephadex LH-20 chromatography, charcoal adsorption chromatography, Bondesil chromatography and HPLC of reverse phase, successively. Biological activities of each purification step were monitored by the rice lamina inclination test. Higher activities against the rice lamina inclination test in the each purification step showed that the shoots of two cultivars biosynthesize brassinosteroids. Two cultivars also showed a similar distribution of biological activities of endogenous brassinosteroids detected by HPLC.

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