• 제목/요약/키워드: fractionation.

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극미세 단백질 분석을 위한 프로테옴 분획 농축 기술 (Prefractionation and Enrichment for the Analysis of Low Aboundance Proteome)

  • 지재웅;변상요
    • KSBB Journal
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    • 제16권5호
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    • pp.435-441
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    • 2001
  • In spite of the powerfulness for the simultaneous study of proteome expression and post-translational modification, 2-D PAGE has inevitable limitation on detect low aboundant proteins. Since many of the low abundant proteins are likely to have very important regulatiory functions in cells, separation and analysis of low copy number proteins is an important issue in proteome studies and challenge for 2-D techniques. Among various methods developed to detect low abundant proteins, electrophoretic protein prefractionation, chromatographic protein prefractionation, and subcellular fractionation are explained in this paper. Their practical strengths and weaknesses are also explained with current research trends.

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NSD와 TDF에 관한 물리적 고찰 (A Biophysical Interpretation of NSD and TDF)

  • 김성규;신세원;김명세
    • 한국의학물리학회지:의학물리
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    • 제1권1호
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    • pp.43-49
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    • 1990
  • On the basis of the review of radiobiological date, a formalism is developed for the analysis and prediction of iso-effect relations for tissue tolerance, which can be used as an alternative to the norminal standard dose(NSD) formaula of Ellis and its derived equations. An important feature of the described formalism is that directly based on radiobiological insights and it provides a more logical concept to account for the diversity of tissue responses. The NSD concept has subsequently been extended to the formalisms of timedose-fractionation(TDF) value. The authors deriveded TDF equation on the basis NSD of Ellis. TDF=0.07(NSD)-26.

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TREATMENT OF CHINESE CABBAGE JUICE

  • Kim, S. H.;N. Proydak;B. S. Shin
    • 한국농업기계학회:학술대회논문집
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    • 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.III
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    • pp.792-802
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    • 2000
  • The coagulation of Chinese cabbage juice can be accomplished by applying the combine method of the formic acid with rate of 3% and in four hours the propionic acid with rate of 1 % in the juice. The separation of coagulation into the protein paste and the brown juice completed in 6.5 hours by set up method in special storage. The protein paste can be stored safely for 30 days in anaerobic condition.

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의성개나리 과피(果皮) 성분(成分)의 항진균작용(抗眞菌作用)에 관한 연구(硏究)(I) (Antifungal Studies on Components from the Pericarp of Forsythia viridissima (I))

  • 노영수
    • 생약학회지
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    • 제6권3호
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    • pp.143-147
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    • 1975
  • The antifungal studies on the triterpenoids from the pericarp of Forsythia viridissima LINDL. were conducted. The pericarp was obtained from the plant which was cultivated at Eusung, Kyungsang-bukkdo, Korea. Substances A and B were isolated by column fractionation and purified by recrystallization. Antifungal actions of substance A, acetyloleanolic acid, show significant inhibition effect against Trichophyton rubrum, T. mentagrophytes, T. tonsurans, Microsporum nanum, M. canis, and M. cookei. Substance B, viridissimic acid, shows negative effect against Candida albicans and shows lower effects against other test fungi.

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Anti-allergy Activity of Cinnamomi Cortex

  • Kim, Ra-Min;Kim, Young-Hee;Lee, Eun-Bang
    • Natural Product Sciences
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    • 제6권1호
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    • pp.49-51
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    • 2000
  • The anti-allergy activity of Cinnamomum cassia cortex extract and its fractions were investigated in mice. The extract exhibited potent anaphylactic shock and Arthus reaction. Among the fractions obtained in the successive fractionation with n-hexane, butanol and acetone, the butanol insoluble portion was shown to have the activities.

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Coumarins from Angelica gigas Roots having Rat Lens Aldose Reductase Activity

  • Lee, Sang-Hyun;Jung, Sang-Hoon;Lee, Yeon-Sil;Shin, Kuk-Hyun
    • Biomolecules & Therapeutics
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    • 제10권2호
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    • pp.85-88
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    • 2002
  • Systematic fractionation of Angelica gigas roots led to the isolation of linear coumarins such as decursinol angelate, decursin and nodakenin. These compounds were tested for their effects on rat lens aldose reductase. Nodakenin was demonstrated to exhibit significant inhibition of rat lens aldose reductase activity with $IC_{50}$ value of $7.33\Mu\textrm{M}$.

The Insecticide Constituents of Several Celastraceae Plants

  • Wang, MingAn;Wu, WenJun
    • 농약과학회지
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    • 제6권1호
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    • pp.9-15
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    • 2002
  • The insecticide constituents of several Celastraceae family plants including Celastrus angulatus, Celastrus orbiculatus, Celastrus flagellaris, Euonymus verrucosides, Euonymus forunei and Euonymus phellomana were studied by bioassay-guided fractionation. All structures of sesquiterpene polyol esters and alkaloids have been elucidated by UV, IR, MS, NMR and x-ray diffraction evidences. These compounds exhibited insecticidal, antifeedant and narcotic activities against Mythimna separata.