• 한국가금학회지
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• 제47권2호
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• pp.115-119
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• 2020

Fowl cholera is an infectious disease caused by Pasteurella multocida that contributes to high economic loss in the commercial chicken industry. Three Pasteurella multocida strains were isolated from outbreaks of acute fowl cholera in the Korean layer farms from 2018 to 2019. One strain was identified and serotyped using capsular PCR typing. This strain was also genotyped by lipopolysaccharide (LPS) PCR typing as A: L3, whereas other strains were non-typable. The multilocus sequence typing (MLST) result showed that the A: L3 strain is sequence type (ST) 134; the non-typable strains were recorded as the following new STs: ST 366 and ST 374. Using phylogenetic tree analysis based on MLST sequences, we determined that ST 366 and ST 374 are closely related to the reference strains that were previously isolated from duck and chicken in Korea, and they were highly prevalent within the Korean cluster. In conclusion, Pasteurella multocida strains were identified and isolated in this study. Furthermore, this is the first report of using MLST to determine the prevalence of fowl cholera in Korea.

• Journal of Microbiology
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• 제44권3호
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• pp.344-353
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• 2006

Symptoms of fowl cholera including orofacial edema, swollen and edematous wattles and combs, and severe respiratory disorders were detected in domestic poultry in two broiler breeder farms: one located in Gyeong-gi Province (October, 2000) and the other in Chung-cheong-nam Province (March, 2001). Gram-negative, bipolar staining bacillus was easily found in a direct smear. The biochemical properties of isolates were examined using a standard diagnosis method, proving that they were 99.7% similar to the Pasteurella multocida (P. multocida: PM), a pathogenic and causative agent of fowl cholera (FC). As a result, an FC outbreak in domestic fowls was confirmed for the first time in Korea since 1942. Because FC was detected in broiler breeder farms for the first time in 59 years at the same time as an FC outbreak was confirmed in wild birds (October, 2000), our concern was focused on whether the PM strains that originated in wild birds were transmitted into poultry forms. The possibility was tracked down by comparing phenotypic and genetic properties between the two types of PM strains. PM strains of chicken origin showed prominent differences from the PM strains of wild bird origin in both phenotypic and genetic properties. An examination of the origin of the wild bird bacteria was conducted, but no evidence has been identified that PM strains from the wild bird were introduced into domestic poultry farms.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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• pp.88-89
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• 2006

Fowl cholera is an infectious disease caused by .Pasteurella multocida, affecting domesticated and wild birds. It usually appears as a septicemia of sudden onset with high morbidity and mortality, but chronic conditions that characterized by localized infections often occur. 13wks broiler breeders were submitted to the Kyung-pook national university for diagnosis. Clinical signs included approximately 1% mortality, severe lameness, ruffled feathers and swollen and/or cloudy eyes. At necropsy, the outstanding lesions were seen swollen hock joint, which were suppurative or caseous exudates, inflammation of conjunctiva, severe pneumonia and epicarditis. The causative agent was isolated from the hock joint, liver, sinus and sternum of the chickens, and performed physiological and biochemical test. To identify the serotype of P. multocida, capsular serotyping was conducted by multiplex polymerase chain reaction (PCR). In the antimicrobial susceptibility test, the isolates were resistance to the aminoglycosides. In this study, we confirmed chronic fowl cholera (FC) caused by P. multocida in broiler breeders in Korea.

• 한국동물위생학회지
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• 제30권1호
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• pp.33-42
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• 2007

Fowl cholera(FC), which has been classified as reportable animal disease in Korea, occurred in the backyard farm located in Chungju, Chungbuk province. The farm was not ordinary domestic poultry farm but backyard farm with main cultivation of fruits. The farmer reported unusual mortality of chickens which were grazed in his own fruit farm without any poultry house. There was a lots of opportunities to contact with wild animals including wild birds freely. Clinical signs observed in the chickens were torticolis, mucous excretion from mouth and greenish diarrhea. On the necropsy we found multifocal necrosis on the surface of liver, hemorrhages in the epicardium and ovary and mucopurulent exudates in the joint. The causative agents was isolated from the liver of chickens collected from the farms and identified as Pasteurella multocida using biochemical tests of bacteria. Also, the isolated bacteria were inoculated into specific pathogen free chickens to evaluate the pathogenicity. High mortality and similar pathological lesions compared to those of chickens died in the farm were observed. We report the first case of fowl cholera of the chickens in Korea and further studies are needed to evaluate the serotype, genotype and pathogenicity of isolated Pasteurella multocida.

• 한국동물위생학회지
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• 제34권4호
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• pp.353-359
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• 2011

Fowl cholera is a contagious acute and chronic disease caused by Pasteurella multocida in both domesticated and wild birds. Acute fowl cholera in both chickens and wild birds has recently been documented in Korea, but the chronic form has not been reported in Korea until now. This study describes the first outbreak of chronic fowl cholera in 13-week-old Arbor Acre broiler breeder chickens submitted to the College of Veterinary Medicine, Kyungpook National University in April 2006. The clinical signs of the affected flock of 9,621 chickens were lameness caused by swollen hock joints, diarrhea, ruffled feathers, and an average weekly mortality of 1.0%. At necropsy, purulent or caseous exudates were found in the hock and wing joints, humerus, and eyes, and severe pneumonia and pericarditis were discovered. Eleven bacterial isolates obtained from the liver, joint, infraorbital sinus and sternal bursa of the submitted chickens were all identified as Pasteurella multocida based on their physiological and biochemical characteristics. Five isolates were examined for antimicrobial susceptibility against 21 different antimicrobial agents including ampicillin. All were resistant to kanamycin, neomycin, and streptomycin, and some were resistant to gentamicin. The tested isolates were all susceptible to the other 17 antimicrobial agents. All 11 isolates were capsular serogroup A based on multiplex polymerase chain reaction. In addition, two of five isolates used in the antimicrobial susceptibility test were identified as somatic serotype 1 by an agar gel diffusion precipitin test, while the others were non-typable.

• 월간양계
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• 제5권10호통권48호
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• pp.97-97
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• 1973

• 월간양계
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• 제4권11호통권37호
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• pp.66-68
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• 1972

• 대한수의학회지
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• 제46권2호
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• pp.127-133
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• 2006

Pasteurella multocida is a terrible veterinary pathogen that causes widespread infections in husbandry. To induce homologous and/or heterologous immunity against the infections, outer membrane protein Hs (OmpH) in the envelope of different strains of P. multocida are thought to be attractive vaccine candidates. Previously we cloned and characterized a gene for OmpH from pathogenic P. multocida (A : 3) (In Press, Korean J. Microbiol. Biotechnol. 2005, 33, December). The gene is composed of 1,047 nucleotides (nt) coding 348 amino acids (aa) with signal peptide of 20 aa. The truncated ompH, a gene without nt coding for the signal peptide, was generated using pRSET A to name "pRSET A/OmpH-F2". This truncated ompH was well expressed in Escherichia coli BL21 (DE3). Truncated OmpH was purified for induction of immunity against live pathogen of fowl cholera (P. multocida A : 3) in mice. Some $50{\mu}g$ of the purified polypeptide was intraperitoneally injected into mice two times with 10 day interval. Lethal dose ($25{\mu}l$) of live P. multocida A : 3 was determined by directly injecting the pathogen into wild mice (n = 25). To demonstrate the vaccine candidate of the truncated OmpH, the live pathogen ($25{\mu}l$) was challenged with the OmpH-immunized mouse group as well as positive & negative controls (n = 80). The results show that the truncated OmpH can be used for an effective vaccine production to prevent fowl cholera caused by pathogenic P. multocida (A : 3).

• 대한수의학회지
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• 제22권2호
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• pp.211-219
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• 1982

닭의 급성(急性) 가금(家禽) 콜레라의 폐사원인(斃死原因)과 기전(機轉)을 구명(究明)하고자 생후(生後) 10~32주령(週齡) 닭에 P. multocida균(菌)을 칠개(七個) 경로(經路)(정맥(靜脈), 근육(筋肉), 피하(皮下), 비강(鼻腔), 구강(口腔), 복강(腹腔) 및 귀)를 통해 주입(注入)해서 가금(家禽) 콜레라를 발병(發病)시키고 파종성(播種性) 혈관내(血管內) 응고(凝固)의 발현여부(發顯與否)와 그 분포(分布) 및 본(本) 질병(疾病) 진행(進行) 과정중(過程中) P. multocida의 endotoxin 역할(役割)에 대하여 연구(硏究)하였다. 파종성(播種性) 혈관내(血管內) 응고(凝固)의 병리조직학적(病理組織學的)인 진단(診斷)은 소동맥(小動脈), 소정맥(小靜脈), 모세혈관(毛細血管) 그리고 다소 큰 혈관내(血管內)에 섬유소성(纖維素性) 혈전(血栓)의 증명(證明)으로 이루어졌다. 각종(各種) 장기내(臟器內) 파종성(播種性) 혈관내(血管內) 응고(凝固)는 주(主)로 3일(日) 이내(以內)에 폐사(斃死)된 닭에서 쉽게 관찰(觀察)할 수 있었고, 장기중(臟器中) 폐(肺)는 혈전(血栓)의 발현빈도(發顯頻度)가 가장 높았으며(90%) 그 다음으로 간(肝)(70%), 신장(腎臟)(60%), 심장(心臟)(20%), 비장(脾臟), 뇌(腦), 췌장(膵臟), 흉선(胸線) 및 갑상선(甲狀腺)의 순(順)이었다. 섬유소성(纖維素性) 혈전(血栓)의 밀도(密度)(조직절편당(組織切片當) 혈전(血栓)의 수(數)) 역시 폐(肺)가 가장 높고 비장(脾臟), 신장(腎臟), 간(肝) 및 심장(心臟)의 순(順)이었다. 급성(急性) 가금(家禽) 콜레라 감염시(感染時) 범발성(汎發性) 출혈(出血)은 파종성(播種性) 혈관내(血管內) 응고(凝固)를 일으키는 P. multocida균(菌)의 endotoxin에 기인(基因)된 것으로 사료(思料)되며 닭의 급성(急性) 가금(家禽) 콜레라의 폐사원인(斃死原因)은 단순(單純)한 출혈성(出血性) 패혈증(敗血症)이 아니라 전신적(全身的)으로 발생(發生)되는 파종성(播種性) 혈관내(血管內) 응고(凝固)를 수반하는 endotoxin(septic) shock사(死)임이 밝혀졌다.

• 한국가금학회지
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• 제19권3호
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• pp.137-150
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• 1992

한국에 있어서 양계산업은 대규모의 현대화된 농장과 오래된 소규모농장이 양립하고 있는 매우 복잡한 양상을 나타내고 있는 상황으로써 이러한 연유로 인해 각종 전염성 질병에 있어서도 혼란 된 역학적 상황이 야기되고 있는 실정이다. 국내에서 경제적으로 중요시되고 있는 주요 가금질병으로는 대사균증, 추백이, 마이코플라즈마 갈리셉티쿰 감염병, 전염성 코라이자, 전염성 활막염등의 세균성 질병, 뉴랫슬병, 계두, 마렉병, 조뇌척수염, 전염성 병, 전염성 후두 기관염, 전염성 기관지염 등의 바이러스성 질병 그리고 원충성 질병으로써 콕시듐병을 들 수 있다. 한편으로 가금 인풀루엔자, 가금콜레라 및 가금티푸스는 최근 수십연간 국내발생이 보고된 바 없으며 외래성 질병으로 간주되고 있다. 본고에서는 국내 발생하고 있는 상기 주요 가금질병들의 역학적 상태, 경제적 영향, 방제방안 및 최근 연구 활동에 대해서 논하였다.