• Asian-Australasian Journal of Animal Sciences
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• v.26 no.5
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• pp.609-624
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• 2013

The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of purebred Xianju hens with consistent high or low shell breakage strength were grouped into two pairs. Using Affymetrix Chicken Array, a whole-transcriptome analysis was performed on hen's shell gland at 9 h post oviposition. Gene ontology enrichment analysis for differentially expressed (DE) transcripts was performed using the web-based GOEAST, and the validation of DE-transcripts was tested by qRT-PCR. 1,195 DE-transcripts, corresponding to 941 unique genes were identified in hens with strong eggshell compared to weak shell hens. According to gene ontology annotations, there are 77 DE-transcripts encoding ion transporters and secreted extracellular matrix proteins, and at least 26 DE-transcripts related to carbohydrate metabolism or post-translation glycosylation modification; furthermore, there are 88 signaling DE-transcripts. GO term enrichment analysis suggests that some DE-transcripts mediate reproductive hormones or neurotransmitters to affect eggshell quality through a complex suite of biophysical processes. These results reveal some candidate genes involved with eggshell strength at the early stage of active calcification which may facilitate our understanding of regulating mechanisms of eggshell quality.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.1029-1031
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• 2015

Objective: Glypican-3 (GPC3) is reported to be an oncofetal protein that is a useful diagnostic immunomarker for hepatoblastoma. However, the results are not inclusive. This study systemically investigated the association between expression of GPC3 and pediatric hepatoblastoma. Methods: Clinical studies evaluating the association were identified using a predefined search strategy. GPC3 immunohistochemistry was applied in the pathological diagnosis of hepatoblastoma using the monoclonal antibodies with formalin-fixed and paraffin-embedded specimens. Positive predictive rates for the association between expression of GPC3 and pediatric hepatoblastoma were calculated. Results: Specimens from four clinical studies which including 134 patients with pediatric hepatoblastoma tested by GPC3 immunohistochemistry were considered eligible for inclusion. Systemic analysis showed that, in all patients, pooled positive predictive rate of the association between expression of GPC3 and pediatric hepatoblastoma was 95.5% (128/134). Conclusion: This systemic analysis suggests that the expression of glypican-3 is highly associated with the diagnosis of pediatric hepatoblastoma.

• Journal of Korean Medical classics
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• v.24 no.3
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• pp.15-25
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• 2011

The acupuncture procedures in Hwangjenaekyung (黃帝內經) was different to modern ones in many aspects. Especially, the role of pulse diagnosis in acupuncture was totally different and the pulse diagnosis was essential part in acupuncture therapy in Hwangjenaekyung era. We found four noteworthy features on the pulse diagnosis before and after acupuncture in Hwangjenaekyung : (1) Pulse diagnosis was a mandatory process in acupuncture (2) Doctors who used pulse diagnosis in 12 meridians (十二經脈遍診脈法), pulse diagnosis in 9 points of 3 body parts (三部九候脈法), and comparative pulse diagnosis between radial artery and carotid artery (人迎寸口對比脈法) followed the former rule ((1)). (3) The major pulse features to detect before and after acupuncture were conversion between the vacuous pulse (虛脈) and the replete pulse (實脈), and conversion between slippery pulse (滑脈) and rough pulse (澁脈). (4) Deukki (得氣, Deqi) was synonym of Kiji (氣至), and it referred to the changes of arterial pulse, not the sensation followed by acupuncture manipulation.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.2
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• pp.143-148
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• 2014

Arecoline is a major alkaloid of areca nuts which are widely chewed by southeast Asian and it manifests various toxic effects in different organs of human and animals. In this work, mature mice were treated by vitamins C plus E, arecoline, or both daily for four weeks. The results showed that arecoline significantly increased the levels of serum alkaline phosphatase (ALP), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and significantly decreased the levels of reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) in the liver tissues. Additionally, the body weight, testis weight, sperm counts, motility and normal sperms also were significantly decreased. The supplement of vitamins C and E can bring the activities of ALP and GPT to normal levels and partially restore the sperm counts compared to the arecoline-treated group but have no other positive effects. In conclusion, the vitamins C and E partially attenuated the arecoline-induced hepatotoxiciy but basically had on protective effects against the arecoline-induced testicular toxicity.

• Steel and Composite Structures
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• v.1 no.1
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• pp.145-158
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• 2001

An experimental study was conducted to evaluate the effects of loading sequence and lateral bracing on the behavior of reduced beam section (RBS) steel moment frame connections. Four full-scale moment connections were cyclically tested-two with a standard loading history and the other two with a near-fault loading history. All specimens reached at least 0.03 radian of plastic rotation without brittle fracture of the beam flange groove welds. Two specimens tested with the nearfault loading protocol reached at least 0.05 radian of plastic rotation, and both experienced smaller buckling amplitudes at comparable drift levels. Energy dissipation capacities were insensitive to the types of loading protocol used. Adding a lateral bracing near the RBS region produced a higher plastic rotation; the strength degradation and buckling amplitude were reduced. A non-linear finite element analysis of a one-and-a-half-bay beam-column subassembly was also conducted to study the system restraint effect. The study showed that the axial restraint of the beam could significantly reduce the strength degradation and buckling amplitude at higher deformation levels.

• Computers and Concrete
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• v.24 no.6
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• pp.513-525
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• 2019

In this work, an experimental research has been performed on Steel Fiber-Steel Reinforced Concrete (SFSRC)specimens subjected to four-point bending tests to evaluate the feasibility of mutual replacement of steel fibers and conventional reinforcement through studying failure modes, load-deflection curves, stiffness of characteristic points, stiffness degradation curves and damage analysis. The variables considered in this experiment included steel fiber volume percentage with and without conventional reinforcements (stirrups or steel fibers) with shear span depth ratios of S/D=2.5 and 3.5. Experimental results revealed that increasing the volume percentage of steel fiber decreased the creation and propagation of shear and bond cracks, just like shortening the stirrups spacing. Higher crack resistance and suturing ability of steel fiber can improve the stability of its bearing capacity. Both steel fibers and stirrups improved the stiffness and damage resistance of specimens where stirrups played an essential role and therefore, the influence of steel fibers was greatly weakened. Increasing S/D ratio also weakened the effect of steel fibers. An equation was derived to calculate the bending stiffness of SFSRC specimens, which was used to determine mid span deflection; the accuracy of the proposed equation was proved by comparing predicted and experimental results.

• Food Science of Animal Resources
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• v.39 no.5
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• pp.704-724
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• 2019

This study mainly investigated the improvement effect of nitroso-hemoglobin (NO-Hb) and four lactic acid bacteria (LAB) (Streptococcus thermophiles, Lactobacillus bulgaricus, Lactobacillus casei, and Montessori enterococcus) on the color and microbiological qualities of raw beef. Three strains of Escherichia coli, Staphylococcus, Salmonella, and Pseudomonas were used as pathogenic bacteria. The results showed that both NO-Hb and LAB could enhance the color stability and scavenged the spoilage bacteria in a minced beef model. But the improvement effect of NO-Hb was more significant than LAB. This suggested that NO-Hb, as a novel ingredient, could be used as a promising substitute for nitrite in meat products to improve the color and safety of meat products. In addition, low field (LF)-NMR method has been established to be practicable to identify changes in the relaxation times of water and fat caused by different type of bacteria and the storage periods. The number of relaxation components in minced beef was affected by bacteria and increase of the storage period.

• Journal of Sasang Constitutional Medicine
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• v.31 no.3
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• pp.9-18
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• 2019

Objectives Sasang Constitutional Medicine(SCM) is uniquely established in the field of Korean Medicine. Taegeuk Acupuncture(TGA) and Eight Constitution Acupuncture(ECA) which are created upon SCM are applied in clinics. However, there exists no established theory but hypothesis with regard to the composition of acupoints of constitution acupuncture. The purpose of this study was to help establish the Sasang constitution acupuncture(SCA) method. Methods Thirties studies were found on various Oriental medicine journals and 17 literatures with the key words relevant to constitution and acupuncture. Results For the principle of acupoint selection, 12 studies were included which were classified into TGA, ECA, SCA, other constitution acupuncture. Among 17 literatures, most of them were relevant to SCA. For the clinical studies, eight of total 13 using ECA were for musculoskeletal diseases. Conclusions Among studies and literatures chosen, the application of Saam's acupuncture method was most frequently found in SCA method. For establishing the SCA method, the two main concepts need to be considered, which are the consideration of four areas namely Lungs, Spleen, Liver and Kidneys and upward-downward-loosening-tightening movement of the Qi according to Sasang constitution type.

• Mycobiology
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• v.48 no.6
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• pp.464-475
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• 2020

Acervus (Pyronemataceae, Pezizales) is a saprobic genus in Pezizomycetes, characterized by colored apothecia, subcylindrical to cylindrical asci and guttulate ascospores. We collected four Acervus samples from China and Thailand. Descriptions and illustrations are introduced for all fresh samples. One new record of A. globulosus from Thailand, one new species, A. rufus, two known species, A. epispartius and A. stipitatus from China are reported. Phylogenetic analysis based on five genes, the large subunit rRNA (LSU), the translation elongation factor-1 alpha (tef1-α), the second largest subunit of RNA polymerase II (rpb2), the largest subunit of RNA polymerase II (rpb1), and the small subunit rRNA (SSU), revealed the distinct position of the new species. The new species is set apart by its red apothecia. A key to Acervus species is also given.

• Journal of the Daesoon Academy of Sciences
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• v.39
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• pp.249-274
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• 2021

The aim of this article is to investigate the religious medical treatments performed by Kang Jeungsan as recorded in The Canonical Scripture, the main scripture of the Korean new religion, Daesoon Jinrihoe. The unity of mind and body is the core principle underlying Kang Jeungsan's religious medical treatments. He believed treating the mind of his patients was more important and necessary than treating their diseases. As such, he believed the only way to treat critical illness was to keep preserve the mind-body unity of his patients. Massage was a physical therapy often used by Kang Jeungsan. The medicinal materials that he commonly used were the Four-substance Decoction (四物湯 samultang), Minor Bupleurum Decoction (小柴胡湯 soshihotang), quince (木瓜 mokgwa *Pseudocydonia sinensis; not papaya), and rehmannia (生地黃 saengjihwang). Kang Jeungsan's religious medical treatments were characteristic of Daoist healing methods. His Daoist style healing methods included multiple techniques such as treating diseases through correcting Qi, exorcising malevolent entities, transferring diseases to a different host, removing a disease through the changing of clothes, pouring medicine into the ground, combining the usage of talisman and incantations, and treating the common people. Among these techniques, Jeungsan's pouring medicine into the ground was especially innovative.