• Title/Summary/Keyword: forming agent

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Inhibition of growth and biofilm formation of Staphylococcus aureus by corosolic acid (Corosolic acid에 의한 Staphylococcus aureus의 생장 및 생물막 형성 저해)

  • Yum, Su-Jin;Kim, Seung Min;Yu, Yeon-Cheol;Jeong, Hee Gon
    • Korean Journal of Food Science and Technology
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    • v.49 no.2
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    • pp.146-150
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    • 2017
  • Staphylococcus aureus is a pathogenic bacterium that causes food poisoning, exhibits a strong capacity to form biofilm, and is highly resistant to antimicrobial agents. The purpose of this study was to investigate the antimicrobial characteristics of corosolic acid against S. aureus. S. aureus showed high susceptibility to corosolic acid in a concentration-dependent manner. The minimum inhibitory concentration and colony-forming ability determined by the broth microdilution method showed that corosolic acid had strong antimicrobial activity against the bacteria. The diameters of the inhibition zone and numbers of colony forming units at each concentration of corosolic acid were also measured. In addition, corosolic acid displayed potent biofilm inhibition activity against S. aureus at concentrations below its minimum inhibitory concentration. These results suggest that corosolic acid can be used to effectively prevent biofilm formation by S. aureus, thereby making S. aureus more susceptible to the action of antimicrobials.

Enumeration of Korean native goat erythrocytes (KGRBC)-rosette forming cells in peripheral blood of Korean cattle (재래산양 적혈구를 이용한 한우 순환 혈액내 rosette 형성 세포 정량)

  • Cheong, Ki-soo;Kim, Neun-su;Kim, Dong-hoon;Kang, Myoung-dai;Song, Hee-jong
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.525-530
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    • 1989
  • In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethylisothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody($EA_{Fc}$)- and erythrocyte-antibody-complement (EAC)-rosetting techniques. The results obtained were as follows; The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette ($6.7{\pm}2.4%$) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8% Dex were formed as $37.3{\pm}2.7%$ and $45.1{\pm}2.1%$, respectively. And the synergistic effects were noted no less than $66.5{\pm}5.6%$ when the KGRBC treated with 0.1M AET and 8% Dex subsequently and rate of RFR did not change significantly between 3~24 hours incubation time at $4^{\circ}C$, EA-and EAC-RFR were $23.3{\pm}9.1%$ and $23.1{\pm}7.9%$, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA- or EAC-rosette assay in cattle-PBL.

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Synthesis and Analysis of Multi-functional Urethane Acrylate Monomer, and its Application as Curing Agent for Poly(phenylene ether)-based Substrate Material (다관능 우레탄 아크릴레이트 단량체의 합성과 분석, 및 폴리페닐렌에테르 기판소재용 경화성분으로의 적용)

  • Kim, Dong-Kook;Park, Seong-Dae;Oh, Jin-Woo;Kyoung, Jin-Bum
    • Polymer(Korea)
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    • v.36 no.4
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    • pp.413-419
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    • 2012
  • Multi-functional urethane acrylate monomers as the curing agent of poly(phenylene ether) (PPE) were synthesized and then the urethane bond formation was checked by FTIR spectrometry and NMR analysis. The synthesized monomers were mixed with PPE and fabricated to dielectric substrates. After forming PPE/monomer composite sheets by a film coater, several sheets were laminated to a test substrate in a vacuum laminator and then its properties depending on the type and the amount of monomers, such as dielectric constant, dielectric loss, and peel strength, were measured. Between the two different hydroxyl acrylates, when the monomer synthesized with 2-hydroxy-3-phenoxypropyl acrylate containing a phenyl group was used as a curing agent, a smaller dielectric loss was obtained and the dielectric constant and loss decreased with a decrease in the amount of the monomer. The peel strength values of the test substrates, however, did not show any specific difference between the cases of two synthesized monomers. As a result, it was obtained the polymer substrate for high frequency application having peel strength of about 10 N, low dielectric constant of 2.54, and low dielectric loss of 0.0027 at 1 GHz.

Understanding Collaborative Working Processes within Construction Project Teams Using Agent-Based Modeling and Simulation (에이전트기반 시뮬레이션을 활용한 건설프로젝트 조직 내 협업과정의 이해)

  • Son, JeongWook;Shin, Seung-Woo;Yi, June-Seong
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.1
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    • pp.70-77
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    • 2014
  • Collaborative processes among team members including communication, coordination, and information-handling processes either during pre-construction or project execution are required in order to accomplish the objectives of construction projects. However, current construction management practice does not explicitly take the effect of organizational aspects on project performance into account. This paper introduces a method to understand collaborative processes in an explicit and systematic fashion. An agent-based simulation of collaborative working processes within construction project teams was designed from game theory perspective and implemented. The simulation produced both individual behavior and network dynamics. Individuals represented as agents made efforts to improve performance by communication and coordinating with other members, and overall team network was emerged as a result of interactions among members. Interestingly, it was found that the tendency of forming cohesive subgroups increased when sustaining relations with between-group partners incurs higher cost. The primary contribution of this paper is that it presented an explicit approach to examining collaborative working processes in construction project teams and it extended existing computational organization and network studies by integrating individual behavior models and network models.

Physical Properties and Virtual Cloth Images of Cotton Fabrics Treated with Chitosan, 1,2,3,4-Butanetetracarboxylic Acid and Citric Acid (키토산과 1,2,3,4-Butanetetracarboxylic Acid, Citric Acid로 가공된 면직물의 역학적 특성과 가상 봉제 이미지)

  • Kim, Kyung-Sun;Jeon, Dong-Won;Kim, Jong-Jun
    • Journal of Fashion Business
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    • v.13 no.1
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    • pp.102-114
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    • 2009
  • Chitosan is a polysaccharide with cationic amino groups in its structure and has useful properties as functional materials. Various end-use developments of chitosan are in progress. When the cotton fabric is pretreated with chitosan, the hand property of cotton fabric may be improved expecially for the summer apparel. In this study, as a cross-linking agent to introduce chitosan into cotton, BTCA(butane-1,2,3,4-tetracarboxylic acid) or CA(citric acid) was added in order to prevent detachment of chitosan by the cross-linking. During the cross-linking procedure, via the padding-drying-heat setting, amino groups of chitosan and hydroxyl groups of cotton, carboxyl groups of BTCA/CA are cross-linked by forming anhydrous cyclic rings. Since BTCA has four carboxyl groups, cross-linking by thermal treatment is easy, leading to the trials in wrinkle-recovery treatment of cotton fabrics. However, the high price of the BTCA reagent has been a shortcoming in the actual application for industrial use. Therefore, in this study, we tried the application of CA having three carboxyl groups, which is relatively low priced, as the substituting cross-linking agent. The hand of the treated fabrics were evaluated by measuring physical properties. In addition, based on the physical properties, three-dimensional images were introduced by using 3D CAD systems and results were compared.

Stability of Oxidizer $H_2O_2$ for Copper CMP Slurry (구리 CMP 슬러리를 위한 산화제 $H_2O_2$의 안정성)

  • Lee, Do-Won;Kim, In-Pyo;Kim, Nam-Hoon;Kim, Sang-Yong;Seo, Yong-Jin;Chang, Eui-Goo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07a
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    • pp.382-385
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    • 2003
  • Chemical mechanical polishing(CMP) is an essential process in the production of copper-based chips. On this work, the stability of Hydrogen Peroxide($H_2O_2$) as oxidizer of Cu CMP slurry has been investigated. $H_2O_2$ is known as the most common oxidizer in Cu CMP slurry. Copper slowly dissolves in $H_2O_2$ solutions and the interaction of $H_2O_2$ with copper surface had been studied in the literature. Because hydrogen peroxide is a weak acid in aqueous solutions, a passivation-type slurry chemistry could be achieved only with pH buffered solution.[1] Moreover, $H_2O_2$ is so unstable that its stabilization is needed using as oxidizer. As adding KOH as pH buffering agent, stability of $H_2O_2$ decreased. However, stability went up with putting in small amount of BTA as film forming agent. There was no difference of $H_2O_2$ stability between KOH and TMAH at same pH. On the other hand, $H_2O_2$ dispersion of TMAH is lower than that of KOH. Furthermore, adding $H_2O_2$ in slurry in advance of bead milling lead to better stability than adding after bead milling. Generally, various solutions of phosphoric acids result in a higher stability. Using Alumina C as abrasive was good at stabilizing for $H_2O_2$; moreover, better stability was gotten by adding $H_3PO_4$.

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Preparation and Evaluation of Low Viscosity Acrylic Polymer Based Pretreatment Solution for DTP Reactive Ink (DTP 반응성 잉크용 저점도 아크릴계 고분자 전처리액 제조 및 특성 평가)

  • Kim, Hyeok-Jin;Seo, Hye-Ji;Kwak, Dong-Sup;Hong, Jin-Pyo;Yoon, Seok-Han;Shin, Kyung
    • Textile Coloration and Finishing
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    • v.29 no.3
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    • pp.122-130
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    • 2017
  • In the direct digital textile printing process, the pre-treatment process is an essential condition for products by forming a clear pattern by sticking and penetration of DTP dye without spreading on the fabric. Recently, pre-treatment agent is changing from high viscosity to low viscosity in order to reduce defects of fabric during pre-treatment process. In this study, pre-treatment agent of acrylic polymer with low viscosity(less than 50cps) was prepared according to the solid content of the polymer, pre-treated on the cotton fabric, and direct DTP printing was performed to compare the color and sharpness. As a result, it showed high color at a viscosity of 50cps or less. When the solid content of the polyacrylic acid having a high molecular weight(A1) was 2.5wt%, when the solid content of the polyacrylic acid having a low molecular weight(A2) was 1 - 1.5wt%, the color was the best. And when the solid content of A1 and A2 was 1.5wt%, the degree of spreading was small and A1 was superior to A2 at the sharpness.

Synthesis, Film Fabrication, and Optical Properties of Polymers Containing Metal Cation Complex Type D-$\pi$-A Chromophore (금속 양이온 배위형 D-$\pi$-A 발색단을 포함하는 폴리머의 합성 및 박막화와 광학특성)

  • Jeong, Seon-Ju;Kim, Hye-Ryun;Yoon, Keun-Byoung;Han, Yoon-Soo;Fujiki, Michiya;Takagi, Akiko;Kwak, Gi-Seop
    • Polymer(Korea)
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    • v.34 no.4
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    • pp.376-380
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    • 2010
  • Donor-$\pi$-acceptor (D-$\pi$-A) type chromophore-based polymers were newly synthesized. These polymers exhibited absorption peak due to intramolecular charge transfer (ICT) in a visible range as well as absorption peak due to carbonyl group in both solution and film state by measuring UV visible spectra. The addition of $Eu^{3+}$ ion into the polymers induced red-shift in absorption due to ICT and the color changes from yellow to red in the solution and film were observed by naked eyes. The contents of crosslinking agent influenced the features and solubility of the polymers. In addition, the contents of crosslinking agent and the $Eu^{3+}$ ion addition improved film-forming ability.

Phytochemical compounds and quality characteristics of spray-dried powders with the blanching condition and selected forming agents from pressed extracts of Ligularia fischeri leaves (블랜칭 처리 및 부형제 종류에 따른 곰취 착즙액 분무건조 분말의 phytochemical 성분 및 품질특성)

  • Kim, Jae-Won;Park, In-Kyung;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.20 no.5
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    • pp.659-667
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    • 2013
  • This study was performed to determine the effects of the blanching condition (immersion ratio 1:15 (w/v) for 3 min at $95^{\circ}C$, and solution containing 1% sodium chloride) and selected forming agents (dextrin DE=10, dextrin DE=20, ${\beta}$-cyclodextrin; each forming agents added 5%) on the phytochemical compounds and quality characteristics of Ligularia fischeri leaves. The moisture was not affected by the forming agent. The color of a, b and chroma values were low in the blanching treatment groups and were significantly lowest with ${\beta}$-cyclodextrin (CD). The polyphenol and flavonoid contents in the blanching treatment groups were higher than those in the non-blanching-treatment group. The ascorbic acid content was higher in the non-blanching-treatment group and was significantly highest in the group treated with dextrin (DE=10) whereas the blanching treatment groups showed lower dehydroascorbic acid content than the non-blanching-treatment group. The water absorption was higher in the non-blanching-treatment group and was significantly highest in the group treated with CD. The water solubility in the blanching treatment groups treated with dextrin (DE=20) and CD was higher than that in the blanching treatment group treated with DE=10. The total chlorophyll and chlorophyll a and b contents were high in the blanching treatment group treated with CD, and for the total carotenoid contents, the same tendency as that seen with the chlorophyll content was observed. With regard to the particle diameter, those in the blanching treatment groups were lower than that in the non-blanching-treatment group and was significantly lowest in the blanching treatment groups treated with DE=20 and CD. The result of SEM observation showed that the spray-dried powders in blanching treatment groups treated with the DE=20 and CD forming agents had uniform particle distribution.

Representative Parameter of Immunostimulatory Ginseng Polysaccharide to Predict Radioprotection (방사선 방어효과 예측 가능한 면역증강 인삼 다당체의 활성인자)

  • Son, Hyeog-Jin;Shim, Ji-Young;Ahn, Ji-Yeon;Yun, Yeon-Sook;Song, Jie-Young
    • Journal of Radiation Protection and Research
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    • v.33 no.3
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    • pp.99-104
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    • 2008
  • According to the increase in the use of radiotherapy to cancer patients, many approaches have been tried to develop new agents for the protection of surrounding normal tissues. However, it is still few applied in the clinic as a radioprotector. We aim to find a representative parameter for radioprotection to easily predict the activity of in vivo experiment from the results of in vitro screening. The polysaccharide extracted from Panax ginseng was used in this study because the immunostimulator has been regarded as one of the radioprotective agent category and was already reported having a promising radioprotective activity through the increase of hematopoietic cells and the production of several cytokines. Mitogenic activity, AK cells activity and nitric oxide production were monitored for the in vitro immunological assay, and endogenous colony-forming unit (e-CFU) was measured as in vivo radioprotective parameter. The immunological activity was increased by the galactose contents of ginseng polysaccharide dependently. The result of this study suggests that mitogenic activity of splenocytes demonstrated a good correlation with in vivo radioprotective effect, and may be used as a representative parameter to screen the candidates for radioprotector.