• Anatomy and Cell Biology
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• v.56 no.1
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• pp.86-93
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• 2023

Age at death estimation has always been a crucial yet challenging part of identification process in forensic field. The use of human skeletons have long been explored using the principle of macro and micro-architecture change in correlation with increasing age. The clavicle is recommended as the best candidate for accurate age estimation because of its accessibility, time to maturation and minimal effect from weight. Our study applies pre-trained convolutional neural network in order to achieve the most accurate and cost effective age estimation model using clavicular bone. The total of 988 clavicles of Thai population with known age and sex were radiographed using Kodak 9000 Extra-oral Imaging System. The radiographs then went through preprocessing protocol which include region of interest selection and quality assessment. Additional samples were generated using generative adversarial network. The total clavicular images used in this study were 3,999 which were then separated into training and test set, and the test set were subsequently categorized into 7 age groups. GoogLeNet was modified at two layers and fine tuned the parameters. The highest validation accuracy was 89.02% but the test set achieved only 30% accuracy. Our results show that the use of medial clavicular radiographs has a potential in the field of age at death estimation, thus, further study is recommended.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7473-7478
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• 2014

Cholangiocarcinoma (CCA) is a fatal cancer with poor prognosis and less than 10% of CCA patients can be offered surgical cure. Conventional chemotherapy results in unfavorable outcomes. At present, plant-derived compounds are gaining interest as potential cancer therapeutics, particularly for treatment-refractory cancers. In this study, antitumor activity of tiliacorinine, the major alkaloid isolated from a tropical plant, on CCA was first demonstrated. Antiproliferative effects of tiliacorinine on human CCA cell lines were investigated using SRB assays. Acridine orange/ethidium bromide staining, flow cytometric analysis and DNA laddering assays were used for apoptotic determination. Apoptosis-related proteins were verified by Western blotting and antitumor activity of tiliacorinine in vivo was demonstrated in CCA xenografted mice. Tiliacorinine significantly inhibited proliferation of human CCA cell lines with $IC_{50}$ $4.5-7{\mu}M$ by inducing apoptosis through caspase activation, upregulation of BAX, and downregulation of $Bcl_{xL}$ and XIAP. Tiliacorinine considerably reduced tumor growth in CCA xenografted mice. These results demonstrated antitumor effects of tiliacorinine on human CCA in vitro and in vivo. Tiliacorinine may be an effective agent for CCA treatment.

• The Korean Journal of Pharmacology
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• v.31 no.2
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• pp.261-269
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• 1995

Background; To explore the efficacy of aspartate as NAD regenerating agent for ethanol and acetaldehyde oxidation, we performed crossover challenge in two groups of volunteers by coadministration of various doses of aspartate, asparagine and ethanol. Methods; 18 healthy male volunteers were randomly divided into two groups. 6 volunteers of the first group were administered 5 gm monosodium aspartate(MSA), 5 gm asparagine or placebo with 100 ml of $40^{\circ}$ whiskey by the 3 way-crossover design, while 12 volunteers of the other group were administered placebo, 1, 2 or 5 bottles of $Aspar^(circledR)$ containing 1 gm of MSA per bottle with 100 ml of $40^{\circ}$ whiskey by the 4 way-crossover design. Ethanol(and acetaldehyde) concentrations in venous blood drawn at 0, 0.25, 0.5, 1, 2, 4, 8th hour after ethanol ingestion were analysed by gas chromatogaphy. Subjective symptoms, liver function tests and psychomotor function tests were also performed during the study periods. Result; Plasma concentration and AUC of acetaldehyde in asparagine and MSA trials on ethanol ingestion were significantly lower than those of placebo trial in the 1st group. Plasma ethanol concentration of 5 bottle $Aspar^(circledR)$ trial was significantly lower than that of placebo trial in the 2nd group. Improvement of subjective symptoms or psychomotor performance by the treatment was not statistically significant. Conclusion; Aspartate and asparagine may be prospective candidates for acceleration of ethanol metabolism and prevention of ethanol toxicity.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.3 no.2
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• pp.321-327
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• 1999

In this progressed super-impose individual recognition system, the photograph of a skull was caught by CCD-camcoder with the MPEG, and an ante-mortem photograph was read by scanner. These two images were processed and superimposed using horizontal angle and vertical angle of face using the forensic dental medicine theory. The enhancement of super-impose individual recognition by anatomical references was performed on the two superimposed images of the same angle using the facial triangle and histogram analysis scheme.

• ALGAE
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• v.26 no.1
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• pp.73-77
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• 2011

A dominant planktonic bloom-forming species, Mallomonas elongata was isolated from a small shallow eutrophic pond. The growth characteristics of this species on variable silicate concentrations and light intensities were investigated in laboratory unialgal cultures. In culture condition of $15^{\circ}C$, the maximum population growth and the highest growth rate of M. elongata occurred at a light intensity of $80\;{\mu}mol\;m^{-2}s^{-1}$, and in culture condition of $18^{\circ}C$, it exhibited the maximum population growth and the highest growth rates at a light intensity of $50\;{\mu}mol\;m^{-2}s^{-1}$. Silicate concentration had no effect on the population growth and growth rate of M. elongata.

• Biomedical Science Letters
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• v.10 no.4
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• pp.333-339
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• 2004

A multiplex PCR assay targeting the yst and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Simultaneous amplification of 145 and 416 bp fragments of the yst and 16S rRNA genes of Y. enterocolitica was obtained using the primer pairs in a single reaction. Validation of the assay was performed with the reference Yersinia strains and other members of the family Enterobacteriaceae. The defined primer pairs amplified the targeted sequence from only pathogenic Y. enterocolitica strains, whereas none of the other bacterial species yielded any amplified fragments. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.

• Anxiety and mood
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• v.10 no.2
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• pp.85-94
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• 2014

Non-pharmacological treatments have long been used for paraphilia or sexual offenders, but few clinical studies were conducted. However, recently there were issued cognitive behavioral therapy of paraphilia or sexual offenders based on the research findings obtained so far. In addition, there were the changes of Diagnostic and Statistical Manual of Mental Disorder by American Psychiatric Association at 2014. The methods of cognitive behavioral therapy for paraphilia or sexual offenders were electric aversion therapy, olfactory aversion therapy, ammonia aversion therapy, covert sensitization, masturbatory reconditioning, directed masturbation, verbal satiation, social skill training, assertiveness training etc. By synthesizing the newly reported foreign guidelines for treatment and review articles, the aim of this study is to investigate the non-pharmacologic therapies used for treatment of paraphilia or sexual offenders.

• Mass Spectrometry Letters
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• v.13 no.1
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• pp.2-10
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• 2022

Metabolomics makes it possible to analyze the interrelationships between various signaling molecules based on the metabolic pathways involved by using high-resolution devices. This approach can also be used to obtain large-scale metabolic information to identify the relevant pathways for disease diagnosis and prognosis and search for potential biomarkers. In the fields of medicine and forensics, hair analysis is used to detect various metabolites in the body. Hair can be harvested readily in a noninvasive manner and is easier to transport and store than blood and urine. Another advantage from a forensic viewpoint is that hair reflects all the components of body fluids. In addition, because of the unique coating structure of hair, it can be used for measurements without changing or destroying its adsorbed components. In this review, the pretreatments for hair analysis, instrumental conditions and clinical applications are discussed. Especially, the clinical use of hair metabolomics in the diagnosis of various diseases and the limitations of the technique are described.

• Journal of Oral Medicine and Pain
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• v.24 no.1
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• pp.81-94
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• 1999

This study intends to find usefulness of the computer assisted dental identification in mass disaster. The variety of dental characteristics was investigated through the research of dental records of 508 adults. And a computer assisted simulation program was used to evaluate the selectivity of dental identification. Findings were as follows : 1. Combinations of dental characteristics were found 155 types. The most various dental characteristic was showed on the mandibular first molar. 99.0% of subjects had dental characteristics for dental identification. 2. The posterior teeth, in comparison with anterior teeth, showed higher selectivity in dental identification which was enhanced by information on the material, type and cavity of the restoration. 3. The variety of dental characteristics was mainly found on the combinations of missing tooth with the material, type and cavity of the restoration. 4. The computer assisted dental identification program, with informa- tion about one's tooth state, made individual identification possible when there was only a part of the teeth in a corpse. 5. The computer assisted dental identification had considerably high selectivity based on the variety of dental characteristics. And it was also performed faster and preciser than the existing identification methods. Based on the results of this study, there are various combinations of the feature of the tooth itself with dental characteristics caused by a certain type of treatment on the teeth. And using the computer assisted dental identification program based on this, dental identification can be more efficient economically and more useful than any other forensic identification methods.

• Journal of Sasang Constitution and Immune Medicine
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• v.8 no.2
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• pp.151-163
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• 1996

VNTR and STR DNA typing are typical genetic analysis methods which are widely used in DNA-fingerprinting for forensic science and other genetic research purposes. In this study, genomic DNA of different constitutions(Taeun, Soyang and Soum) were analyzed by VNTR and STR DNA typing to provide scientific and objective references for Sasang Medicine. It was found out in this study that VNTR-MCT118 and YNZ22 loci showed too many different variation of allele distribution and numbers for each constitution. Therefore, it is thought that VNTR typing can not used for genetic classification study for Sasang Constitution which classifies human body into 4 groups. However, vWA locus, one of the STR loci investigated in this study, showed slight difference in allele distribution for each different constitution.