• BMB Reports
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• v.48 no.10
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• pp.583-588
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• 2015

Microtubule actin crosslinking factor 1 (MACF1), a widely expressed cytoskeletal linker, plays important roles in various cells by regulating cytoskeleton dynamics. However, its role in osteoblastic cells is not well understood. Based on our previous findings that the association of MACF1 with F-actin and microtubules in osteoblast-like cells was altered under magnetic force conditions, here, by adopting a stable MACF1-knockdown MC3T3-E1 osteoblastic cell line, we found that MACF1 knockdown induced large cells with a binuclear/multinuclear structure. Further, immunofluorescence staining showed disorganization of F-actin and microtubules in MACF1-knockdown cells. Cell counting revealed significant decrease of cell proliferation and cell cycle analysis showed an S phase cell cycle arrest in MACF1-knockdown cells. Moreover and interestingly, MACF1 knockdown showed a potential effect on cellular MTT reduction activity and mitochondrial content, suggesting an impact on cellular metabolic activity. These results together indicate an important role of MACF1 in regulating osteoblastic cell morphology and function.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.722-727
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• 2016

In this study, we attempted to reduce the generation of waste acids in the electropolishing process by improving the current efficiency. The optimum conditions of the electropolishing process when using the round bus bar were determined by the Taguchi method. The current density, polishing time, electrolyte temperature and flow rate were selected as the control factors for the current efficiency in the electropolishing process. An orthogonal array was created by considering three levels for each factor and experiments were carried out. The larger-the-better SN ratios were calculated by the Taguchi method. The current density was the most important factor affecting the current efficiency and the polishing time was the least important one. The optimum conditions to minimize the generation of waste acids were a current density of $45A/dm^2$, polishing time of 4 min, electrolyte temperature of $65^{\circ}C$ and flow rate of 7 L/min. The results of the ANOVA confirmed that the effects of the current density, electrolyte temperature and flow rate are significant at the 95% confidence level. The increase in the contact area and contact force afforded by using the round bus bar improved the current efficiency which, in turn, reduced the amount of waste acids generated. Further research is planned to investigate the effect of the type of bus bar on the current efficiency.

• Journal of Korean Tunnelling and Underground Space Association
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• v.14 no.4
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• pp.337-356
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• 2012

Three-dimensional (3D) numerical analyses have been performed to study the behaviour of single piles and grouped piles to adjacent tunnelling in the lateral direction of the pile. In the numerical analyses, the interaction between the tunnel, the pile and the soil next to the piles and shear transfer mechanism have been analysed allowing soil slip at the pile-soil interface by using interface elements. The study includes the shear stresses at the soil next to the pile, the axial force distributions on the pile and the pile settlement. It has been found that existing elastic solutions may not accurately estimate the pile behaviour since several key issues are excluded. Due to changes in the shear transfer between the pile and the soil next to the pile with tunnel advancement, the shear stresses and axial force distributions along the pile change drastically. Downward shear stress develops above the tunnel springline while upward shear stress is mobilised below the tunnel springline, resulting in a compressive force on the pile. In addition, mobilisation of shear strength at the pile-soil interface was found to be a key factor governing pile-soil-tunnelling interaction. It has been found that grouped piles are less influenced by the tunnelling than the single pile in terms of the axial pile forces. The reduction of apparent allowable pile capacity due to pile settlement resulted from the tunnelling seemed to be insignificant.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.32 no.2
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• pp.95-105
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• 2020

In this study, using the CADMAS-SURF model, the characteristics of the wave pressures and the wave forces were analyzed according to the installation position of the parapet on top of the caisson, and the stability evaluation was carried out using estimated wave forces for the design wave condition. Numerical results show that adopting the rear-parapet reduces the front maximum wave pressures and wave forces, and the maximum wave pressure acting on the rear-parapet increases slightly compared to the front parapet, but the wave force acting on the rear-parapet has little effect on the stability of the breakwater due to the phase difference with the wave force acting on the front of the breakwater. In addition, impulsive wave pressures did not occur, as Yamamoto et al. (2013) pointed out the problem of the rear-parapet breakwater. As a result of the stability against sliding and overturning, it was estimated that the target safety factor of 1.2 could be secured by the self-weight of 13% less than the case of the front parapet. At this time, the maximum ground pressure was also reduced by 30%, and the applicability of the rear-parapet structure to the actual site was evaluated as high.

• Journal of the Korean GEO-environmental Society
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• v.16 no.9
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• pp.33-41
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• 2015

The vertical bearing capacity of a bucket foundation installed in sand can be calculated as sum of the skin friction and end bearing capacity. However, the current design equations are not considering the non-associated flow characteristics of sand and the reduction in the skin friction and increase in the end bearing capacity when the vertical load is applied. In this study, we perform two-dimensional axisymmetric finite element analyses following non-associated flow rule and calculate the vertical bearing capacity of circular bucket foundation of various sizes installed in sand of different friction angles. After calculating the skin friction and end bearing force at the ultimate state, design equations are derived for each. The skin friction of bucket foundation is shown significantly small compared to the end bearing capacity. Considering the difference with the available design equation for piles, it is recommended that the equation for piles is used for the bucket foundation. A new shape-depth factor ($s_q{\cdot}d_q$) for bucket foundation is recommended which also accounts for the increment of the end bearing capacity due to skin friction. Additionally, the shape and depth factor of embedded foundation proposed from the associated flow rule can overestimate the bearing capacity in sand, so it is more adequate to use the shape-depth factor proposed in this study.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.6 no.2
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• pp.267-276
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• 1999

The purpose of this study was to identify risk factors predictive of alterations in skin integrity during the intraoperative period. The predictive risk factors were studied for intraoperative pressure sores from December 1998 through January 1999. A sample of 220 patients was selected from the operating room schedule of a University Hospital in Pusan. There were two criteria in including patients : the operation lasted longer than 2 hours and the absence of skin break-down according to NPUAP criteria. The data were analized by SPSS/PC, Stepwise multiple logistic regression was used to identify the variables which were predictive of alterations in skin integrity. Of the 220 patients studied, 41 patients (18.6%) developed stage 1 pressure sores in the immediate postoperative period. In relation to skin changes, three independent variables emerged from the stepwise multiple logistic regression as being significant (p<0.05). Factors predictive of pressure sore formation included low serum albumin(p=0.000), prone position while undergoing surgery(p=0.0004), time on the operating table(p=0.0165). Among the intrinsic factors, serum albumin was the most significant causal factor in pressure sores development in the intra-operative period. Pressure and shearing force were the most significant extrinsic factors in pressure sores development. From the results of this study we concluded that the primary nursing goal is the maintenance of the proper patient' position during the intraoperative period. Also imperative for sore prevention is the reduction of surgery time and improving preoperative nutritional status.

• Journal of Advanced Research in Ocean Engineering
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• v.1 no.4
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• pp.227-238
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• 2015

The present work was an attempt to investigate the applicability of truncated mooring systems to KRISO's deep ocean engineering basin (DOEB) with ratios of 1:100, 1:60, and 1:50. The depth of the DOEB is 15 m. Therefore, the corresponding truncated depths for this study were equal to 1500 m, 900 m, and 750 m. The investigation focused on both the static and dynamic characteristics of the mooring system. It was shown, in a static pull-out test, that the restoring force of a FPSO vessel could be modified to a good level of agreement for all three truncation cases. However, when the radius of the mooring site was reduced according to the truncation factor, the surge motion response during a free-decay test showed a significant difference from the full-depth model. However, the reduction of this discrepancy was achieved by increasing the radius up to its maximum possible value while considering the size of the DOEB. Especially, in terms of the time period, the difference was reduced from 24.0 to 5.3 s for a truncation ratio of 1:100, 54.1 to 8.6 s for a truncation ratio of 1:60, and 31.7 to 3.9 s for a truncation ratio of 1:50. As a result, the study verified the applicability of the truncated mooring system to the DOEB, and therefore it could represent the full-depth mooring system relatively well in terms of the static and dynamic conditions.

• Journal of the Korean Society of Safety
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• v.28 no.5
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• pp.41-48
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• 2013

This paper is intended to provide the background information and justification for Korean highway bridge design code(limit state design)(2012). Limit state design method calculates reliability index and probability of failure through the analysis of the reliability of the experimental database. It has become possible to perform the economical and consistent design by evaluating the safety of a structure quantitatively. In this paper, we used the design specifications of RC slab bridge of superstructure form of Road Design Manual in Part 5 bridge built in highway bridge. This study conducted structural analysis using the method of frame structure theory, design and analysis of bridge by limit state design method, the design code including various standards and Load model applied Korean highway bridge design code limit state design(KHBDC;2012). As a result, it analyzed the effect of safety through comparison. Showing effect of improvement the safety factor and comparing the value of the result, it is determined to be capable of economical design and safety. Furthermore, limit state design method was able to determine many redundant force of cross-section compared with existing design method. It is determined that it can reduce the overall amount because of the reduction of the cross-section and girder depth.

• Plant Journal
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• v.4 no.3
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• pp.54-59
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• 2008

The resonance of boiler is caused by exciting force in the gas path and it generates the vibration by the harmony of boiler's dimensional factor. According to trending toward the boiler of increasing capacity and a bigger size, it has a problem of the vibration at back-pass heating surfaces. We can predict such vibrations as comparison between vortex frequency and gas column's natural frequency. We can't rely on the method for the past decades because of changing parameters, such as an allowable error, gas temperature, gas velocity, Strouhal number. We can reduce the vibration to use the seasoning effect and change the operating condition in coal fired boiler but it's not essential solution. When the vibration occurred in the model boiler, we must measures the acoustic pressure and frequency of places for considering the means. So far, we confirmed the problem from field measures and theoretical analysis about the acoustic vibration of boiler. We installed anti-acoustic baffle in a existing boiler to change the acoustic natural frequency at the cavity, which results in reducing the acoustic vibration. The first, we prove that the acoustic resonance is caused by harmonizing vortex shedding frequency of tube heat surface with acoustic natural frequency of cavity in the range of 650~750 MW loads. The second, the acoustic resonance at the back-pass heating surface has the third order of acoustic natural frequency at the second economizer. We install five anti-acoustic baffles at the second economizer to reducing the resonance. We confirm considerably reducing the acoustic vibration of boiler during the commercial boiler.

• Journal of Distribution Science
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• v.17 no.5
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• pp.103-111
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• 2019

Purpose - This paper aims to examine a spirituality's impact on the posttraumatic interpersonal relationships of family violence with a structural equation model(SEM). This article starts with the research idea that family violence experience bring about negative emotion in terms of interpersonal relationship leading to the distribution of negative affect in social relationship. The research question of this study pinpoints on how family violence experience affects interpersonal relationship problems and how spirituality affects interpersonal relations problems. Based on the idea, we attempts to deal with spirituality's impact as a driving factor of transforming the distribution of negative affect. Research design, data, and methodology - The structural equation modelling(SEM) is employed as a analysis method of this study. SEM provides a chiefly cross-sectional statistical modelling method, and moreover it allows multiple variables to be associated with a latent variable in this analysis. 300 samples from Seoul, Gyeonggy and Gangwon are selected in the survey. We chose available 271 among them. Results - The results verify that trauma have placed an negative impact on personal relations. The study results also shows that the more serious get the degree of family violence trauma, the worse become interpersonal relationship problems. What is more interesting is that the degree of trauma's impact on interpersonal relation problems in male is higher than that of trauma's impact in female. It is mainly attributed to the fact that women are superior to men in terms of empathy ability and interpersonal relationship orientation. It can be noted that the well-managed spirituality will be able to make a great contribution towards reducing the degree of trauma caused by interpersonal relationships. Conclusions - The study results demonstrates that family violence have negative impacts on spirituality, while spirituality have an positive impacts on reduction the problems of interpersonal relationship. It has been found out that family violence trauma may cause negative affectivity in personal relations, which can distribute negative impacts regarding interpersonal relationship in society. This study also supports that spirituality will be able to function well as a helper, which may reduce the distribution of negative affect in society.