• 한국초지조사료학회지
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• 제32권2호
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• pp.101-108
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• 2012

유채 품종에서 황 공급수준이 황산염 흡수 및 동화에 미치는 영향을 구명하고자 어린 잎과 성엽 조직에서 ATP sulfurylase (ATPs), ${SO_4}^{2-}$ 흡수, 글루타치온 함량을 분석하였다. 본 실험에서 10가지 유채 품종들 (Mosa, Capitol, Saturnin, Akela, Pollen, Mokpo, Youngsan, Tamra Colosse 그리고 Naehan)은 황 공급수준에 따라 몇 가지 황산염 흡수와 동화 능력이 다르게 나타났다. 황 결핍 조건에서 모든 품종의 ATP sulfurylase (ATPs) 활력은 늙은 잎에 비해 어린 잎에서 높게 나타났으며, 글루타치온 함량은 황 공급수준이 감소함에 따라 어린 잎에서 많이 증가하였다. 이러한 결과들은 유채 품종별 황 결핍조건에서 황을 이용하는 능력이 다르다는 것을 잘 보여주었다.

• 한국초지조사료학회지
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• 제28권2호
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• pp.71-74
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• 2008

유채 품종간의 황 이용률을 비교하기 위하여 10가지 유채 품종(Mokpo, Tamra, Youngsan, Naehan, Saturnin, Akela, Mosa, Capitol, Pollen and Colosse)을 정상적인 황 공급(2.0 mM) 조건하에서 재배하여 품종별로 황 흡수량을 측정하였다. 또한 유채 품종간의 생육 특성을 비교하기 위해 잎의 수, 잎의 길이, 잎의 넓이 및 뿌리의 길이를 조사하였다. 그 결과, 모든 유채품종에서 잎의 수, 잎의 길이와 넓이는 별다른 차이는 나타나지 않았다. 뿌리 길이는 Saturnin에서 36.3cm로 가장 길게 나타났다. Saturnin, Youngsan과 Mokpo에서 ${SO_4}^{2-}$ 흡수량이 대체적으로 높았고 Mosa와 Pollen에서 ${SO_4}^{2-}$ 흡수량 상대적으로 낮았다. ${SO_4}^{2-}$ 흡수량이 높은 Saturnin와 Mokpo에서 ${NO_3}^-$ 흡수량도 높게 나타났다.

• 한국초지조사료학회지
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• 제43권1호
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• pp.56-61
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• 2023

The germination process is critical for plant growth and development and it is largely affected by environmental stress, especially salinity. Recently, hydrogen sulfide (H₂S) is well known to act as a signaling molecule in a defense mechanism against stress conditions but poorly understood regulating seed germination. In this study, the effects of NaHS (the H₂S donor) pretreatment on various biochemical (hydrogen peroxide (H₂O₂) content and amylase and protease activity) and physiological properties (germination rate) during seed germination of oilseed rape (Brassica napus L. cv. Mosa) were examined under salt stress. The seed germination and seedling growth of oilseed rape were inhibited by NaCl treatment but it was alleviated by NaHS pretreatment. The NaCl treatment increased H₂O₂ content leading to oxidative stress, but NaHS pre-treatments maintained much lower levels of H₂O₂ in germinating seeds under salt stress. Amylase activity, a starch degradation enzyme, significantly increased over 2-fold in control, NaHS pretreatment, and NaHS pretreatment under NaCl during seed germination compared to NaCl treatment. Protease activity was highly induced in NaHS-pretreated seeds compared to NaCl treatment, accompanied by a decrease in protein content. These results indicate that NaHS pretreatment could improve seed germination under salt stress conditions by decreasing H₂O₂ accumulation and activating the degradation of protein and starch to support seedling growth.

• 한국초지조사료학회지
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• 제16권1호
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• pp.1-8
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• 1996

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Forage rape (Brussicu napus L.) was sown at 10 day> interval from Sep. I to Nov. 1. Field-grown palnts were sampled on the before wintering (Dec. 4) and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. On the before wintering, the dry matter yields from the plots sown on Sep. 1, Sep.11 and Sep. 21 were 860, 596 and 260 kg/lOa, respectively. No. harvest was canied out on the plots sown after Oct. 1 because the growth state was not enough to cut. Both of nitrogen and starch contents per plant significantly increased as the seeding date was later. On the wintering period, the contents of nitrogen reserves in roots were 176.8. 120.1, 71.7, 84.0, 72.1, 45.3 and 33.3 mg/plant, those of starch reserves were 199.0. 55.8, 21.8, 92.6, 86.5, 36.4 and 29.0 mglplant, resepctively, in the plots sown on Sep. 1, Sep. 11, Sep. 21, Oct. I, Oct. 11, Oct. 21 and Nov. 1. The rates of winter survival were 40, 36, 33, 85, 87, 59 and 49% and regrowth yields were 161, 86, 65, 520, 451, 121 and 33 kgD.M/lOa, respectively, in each plots. 'Ihese results clearly showed that seeding date has a close influence on the level of organic reserves and forage yield, and that the possibility of continuous utilization on the before and alter wintering is extremely limited by seeding date.

• 한국초지조사료학회지
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• 제14권4호
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• pp.324-330
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• 1994

The objective of this study is to obtain the basic data for investigating the potentiality of continuous utili~ation (first cutting in the late fall and regrowth yield in next spring) of forage rape seeded in fall. Fresh yield and the content of nitrogenous compound in leaves and roots were measured during a growth period. Fresh yield accumulation was very low between the late fall(470kg/10a) and the early regrowth period( 1.070kg /IOa). Total nitrogen content in leaves until the wintering period was decreased from 4.71% to 3.70%. while that in roots slightly increased during this period. The highest content(4.84%) in roots was observed in the early regrowth period, and then rapidly decreased as growth advanced. Protein-N was the largest pool of nitrogenous compound in leaves and roots through entire growth period, Its content in leaves decreased until the wintering period (Feb. 4), and then increased until the bolting stage(Apr. 10). Protein-N in roots highly accumulated from the late fall(11.1mg/gDM) to the early regrowth period(l6.9mg/gDM), and then decreased until the early tlowering stage. The content of amino acid-N in leaves showed a little change with a range from 5.7 to 8.5mgIgDM during entire growth period, while that in roots rapidly decreased from early regrowth period. The content of $NO_3$-N decreased from 7.0 to 4.3mglgDM in leaves, while increased from 0.9 to 2.3mg/gDM in roots from the late fall to the wintering period. 7he content of $NH_4$-N was lower and less varillble than other nitrogen compound during entire growth period. The results clearly showed that protein-N was the main storage form and highly accumulated in roots of overwintering forage rape.

• 한국초지조사료학회지
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• 제15권4호
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• pp.231-237
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survival or regrowth yield. Forage rape (Brassica napus L.) was sown by three seeding densities of 5, 15 and 25cm interval among plants on Sep. 1, 1994. Field-grown plants were sampled on the before wintering (Dec. 4) and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. The dry matter yields from the plots of 5, 15 and 25cm seeding interval were 1,270, 1.01 9 and 1,062 kg/lOa respectively, on the before wintering. The similar pattern wa5 observed in the crude protein yields affected by seeding density. On the before wintering, both of nitrogen and starch contents per plant significantly increased as the seeding density was lowered. Starch content was relatively higher than that of nitrogen in all plots. On the wintering period, the contents of nitrogen reserves were 6.5, 41.2 and 121.7 mglplant, those of starch reserves were 1.0, 5.4 and 185.1 mg/plant, respectively, in the plots of 5, 15 and 25cm seeding interval. Nitrogen reserves on the wintering period increased while starch reserves highly decreased in all plots comparing to the before wintering. 'lhe rates of winter survival were 10.2, 20.6 and 37.1%, and regrowth yields were 76, 96 and 178 kgD.M/ IOa, respectively, in the plots of 5, 15 and 25cm seeding interval. These results cleariy showed that seeding density have a close influence on the level of nitrogen and non-structurd cahohydrate reserves, and that the rate of winter survival and regrowth yield were controlled by reserves level on the wintering period.

• 한국초지조사료학회지
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• 제15권4호
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• pp.238-244
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Forage rape(Brassica napus L.) was sown on Sep. 1, 1994. Experimental plots were divided into three replicates under 6 different cutting dates(l0 days interval from Oct. 15 to Dec. 4). Field-grown palnts were sampled on the each cutting date and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. On the before wintering, dry matter yields were 152, 274, 500, 718, 776 and 981 kg/lOa, respectively, from the cutting date on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. Cmde protein yield significantly increased as cutting date was later until Nov. 14, thereafter a significant increase did not occured. Nitrogen and starch contents per plant significnatly increased as the cutting date was later. The increasing rate of starch was greatly higher than that of nitrogen. On the wintering period, nitrogen reserves in mts were 85.3, 68.8, 47.6, 28.3, 44.3, and 55.3 mglplant, and starch reserve were 11 1.3, 75.3, 39.3, 19.6, 26.4 and 34.6 mglplant, respectively, in the plots cut on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. It showed that carbohydrate reserves were much highly utilized than nitrogen reserves during wintering period. The rates of winter survival were 91, 83, 46, 22, 35 and 43% and regrowth yields were 692, 545, 316, 84, 127 and 140 kgD.M/lOa, respectively, in each plots. The highly significant correlation (p<0.01) between the level of organic reserves and the rate of winter survival or regrowth yield were obtained.

• 한국초지조사료학회지
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• 제32권4호
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• pp.343-352
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• 2012

Sulphur deficiency has become widespread over the past several decades in most of the agricultural area. Oilseed rape (Brassica napus L.) is a very sensitive to S limitation which is becoming reduction of quality and productivity of forage. Few studies have assessed the sulphur mobilization in the source-sink relationship, very little is known about the regulatory mechanism in interaction between sulphur and nitrogen during the short-term sulphur deficiency. In this study, therefore, amount of sulphur and nitrogen incorporated into amino acids and proteins as affected by different S-supplied level (Control: 1 mM ${SO_4}^{2-}$, S-deficiency: 0.1 mM ${SO_4}^{2-}$, and S-deprivation: 0 mM ${SO_4}^{2-}$) were examined. The amount of sulphur in sulphate (S-sulphate) was significantly decreased by 25.8% in S-deprivation condition, compare to control, but not nitrogen in nitrate (N-nitrate). The markedly increase of sulphur and nitrogen incorporated amino acids (S-amino acids and N-amino acids) was observed in both S-deficiency and S-deprivation treatments. The amount of nitrogen incorporated proteins (N-protein) was strongly decreased as sulphur availability while the amount of sulphur incorporated into proteins (S-protein) was not affected. A highly significant ($p{\leq}0.001$) relationship between S-sulphate and S-amino acid was observed whereas the increase of N-amino acids is closely associated with decrease of N-proteins. These data indicate that increase of sulphur and nitrogen incorporated into amino acids was from different nitrogen and sulphur metabolites, respectively

• 한국초지조사료학회지
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• 제13권2호
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• pp.122-131
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• 1993

This study was conducted in 1991 and 1992 to determine the effects of winter annual forage corps such as winter rye (Secale cereale L.), rape (Brassica napus subsp. oleifera L.), oats (Auena satiua L.), crimson clover (Trifolium incarnatum L.), vetch (Vicia satiua L.) and Italian ryegrass (Lolim multiflorum Lam.) on subsequent growth, yield and quality of silage corn (Zea mays L.). The winter annual forage crops were seeded at the end of August and harvested in the fall and spring before corn planting. Corn-leaf numbers, plant heights and LA1 were generally reduced during early development when corn followed Italian ryegrass(P<0.05) and winter rye, but the silking date of corn was not affected by the winter annual forage crops. Corn dry matter and TDN yields were significantly reduced when corn followed Italian ryegrass and late maturing Kodiak winter rye. The yield reductions for corn following Italian ryegrass and Kodiak winter rye, relative to corn which did not follow the winter annual forage crops, were 49 and 19% respectively. but the corn yield was slightly improved by 2 and 3%, respectively. when corn followed crimson clover and vetch. No quality differences in corn stover were observed among the treatments except for the lowest ADF and NDF contents and the highest IVDMD where corn followed ltaliabn ryegrass.

• 한국초지조사료학회지
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• 제28권2호
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• pp.75-80
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• 2008

유채의 황 이용성에 대한 영향을 조사하기 위하여 유채 2 품종(cv. Akela, Colosse)을 2.0mM ${SO_4}^{2-}$와 0.2mM ${SO_4}^{2-}$에서 ${SO_4}^{2-}$ 흡수, ATP sulfurylase의 활성과 염조직내의 glutathione(GSH) 함량을 측정하였다. 0.2mM ${SO_4}^{2-}$에서 두 품종 모두 2.0mM ${SO_4}^{2-}$에 비해 현저하게 낮은 ${SO_4}^{2-}$ 흡수율을 나타냈다. 0.2mM ${SO_4}^{2-}$에서 APT sulfurylase의 활성은 어린잎에서 두 품종 모두 증가하였으나 성숙된 잎에서의 활성은 큰 변화가 얼었다. 0.2mM ${SO_4}^{2-}$에서 glutathione의 함량은 어린잎에서 두 품종 모두 증가하였으나 성숙된 잎에서는 Akela에서만 현저하게 감소하였다. 이러한 결과들은 유채품종간의 ${SO_4}^{2-}$ 흡수와 ATP sulfurylase의 특이성 뿐만 아니라, ${SO_4}^{2-}$ 흡수가 glutathione과 같은 황을 포함하고 있는 화합물로의 동화와 밀접한 관련이 있음을 나타낸다.