• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.1059-1064
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• 2006

Six muscles were seamed out randomly from Hanwoo carcasses (n = 12) of each quality grade (quality grades 1, 2 and 3). Samples were analysed for their total and soluble collagen contents, IMCT (intramuscular connective tissue) and Warner-Bratzler shear force (WBSF). Simple correlation (n = 21) was determined for WBSF among major muscles. For LT (longissimus thoracis), total collagen content was significantly higher (p<0.05) for quality grade 3 than those for quality grades 1 and 2. For semitendinosus (ST), semimembranosus (SM), psoas major (PM) and serratus ventralis (SV), total collagen content of quality grade 1 was lowest (p<0.05) of all quality grades. IMCT shear force for gluteus medius (GM) decreased (p<0.05) with better quality grade, and those for other muscles, with the exception of GM, were higher (p<0.05) for quality grade 3 than for quality grades 1 and 2. WBSF values showed GM and LT to be decreased (p<0.05) with better quality grade, and PM to be higher (p<0.05) for quality grade 3 than those for quality grades 1 and 2. SM, ST and SV from quality grade 1 had lower (p<0.05) WBSF value than those from quality grades 2 and 3. Total collagen content of ST was highest (p<0.05) of all muscles, whereas that of PM was lowest (p<0.05). Soluble collagen contents of LT and SV from quality grades 1 and 2 were, in general, higher (p<0.05) than other muscles, but that of SM was lowest (p<0.05). ST and SM had higher (p<0.05) WBSF values for three quality grades when compared to other muscles, whereas PM was lowest (p<0.05). LT had the strongest simple correlation with SV (r = 0.78) and GM (r = 0.77), and SM had the strongest correlation with ST (r = 0.73) and LT (r = 0.73). Also, PM had the strongest correlation with SV (r = 0.62).

• Journal of Life Science
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• v.31 no.11
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• pp.995-1003
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• 2021

Chromatin remodeling regulates gene expression through epigenetic mechanisms. Aberrations in histone modification have been associated with depression-like behaviors in animal models. Additionally, growing evidence also indicates that epigenetic modification is associated with depression. p11 (S100A10) has been implicated in the pathophysiology of depression both in human and rodent models. In the present study, we investigated alterations in histone acetylation and methylation at the promoter of the p11 gene in the hippocampus of mice subjected to chronic unpredictable stress (CUS). C57BL/6 mice were exposed to CUS daily for 3 weeks. Depression-like behaviors were measured with the forced swimming test (FST). The levels of hippocampal p11 expression were analyzed by quantitative real-time polymerase chain reaction (PCR) and Western blotting. The levels of acetylated and methylated histone H3 at the promoter of p11 were measured by chromatin immunoprecipitation followed by real-time PCR. CUS-exposed mice displayed depression-like behaviors with prolonged immobility in FST. CUS led to significant decreases in the expression of p11 at both protein and mRNA levels. Meanwhile, there was a decrease in histone H3 acetylation (Ac-H3) and H3-K4 trimethylation (H3K4met3) and an increase in H3-K27 trimethylation (H3K27met3) at the p11 promoter. These results indicate that chronic stress causes the epigenetic suppression of p11 expression in the hippocampus.

• Molecules and Cells
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• v.45 no.3
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• pp.122-133
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• 2022

The aim of this study was to investigating whether lncRNA H19 promotes myocardial fibrosis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis. Patients with atrial fibrillation (AF) and healthy volunteers were included in the study, and their biochemical parameters were collected. In addition, pcDNA3.1-H19, si-H19, and miR-29a/b-3p mimic/inhibitor were transfected into cardiac fibroblasts (CFs), and proliferation of CFs was detected by MTT assay. Expression of H19 and miR-29a/b-3p were detected using real-time quantitative polymerase chain reaction, and expression of α-smooth muscle actin (α-SMA), collagen I, collagen II, matrix metalloproteinase-2 (MMP-2), and elastin were measured by western blot analysis. The dual luciferase reporter gene assay was carried out to detect the sponging relationship between H19 and miR-29a/b-3p in CFs. Compared with healthy volunteers, the level of plasma H19 was significantly elevated in patients with AF, while miR-29a-3p and miR-29b-3p were markedly depressed (P < 0.05). Serum expression of lncRNA H19 was negatively correlated with the expression of miR-29a-3p and miR-29b-3p among patients with AF (r_s = -0.337, r_s = -0.236). Moreover, up-regulation of H19 expression and down-regulation of miR-29a/b-3p expression facilitated proliferation and synthesis of extracellular matrix (ECM)-related proteins. SB431542 and si-VEGFA are able to reverse the promotion of miR-29a/b-3p on proliferation of CFs and ECM-related protein synthesis. The findings of the present study suggest that H19 promoted CF proliferation and collagen synthesis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis, and provide support for a potential new direction for the treatment of AF.

• KIEE International Transactions on Electrophysics and Applications
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• v.4C no.2
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• pp.73-76
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• 2004

0-3 PbTiO$_3$/P(VDF/TrFE) nanocomposite thin films for passive pyroelectric infrared sensors were fabricated by a two-step spin coating technique. 65wt% VDF and 35 wt% TrFE was formed into a P(VDF/TrFE) powder. Nano size PbTiO$_3$ powder was used. 0-3 connectivity of PbTiO$_3$/P(VDF/TrFE) composite film was successfully achieved and observed using SEM photography. The dielectric constant and pyroelectric coefficient were measured and compared with P(VDF/TrFE). A very low dielectric constant (13.48 at 1KHz and sufficiently high pyroelectric coefficient (3.101 nC/$\textrm{cm}^2$ㆍk at 5$0^{\circ}C$) were measured. This nanocomposite can be used for a new pyroelectric infrared sensor to achieve better performance.

• Research in Community and Public Health Nursing
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• v.18 no.2
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• pp.310-319
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• 2007

Purpose: This study intends to investigate gender role identity and egalitarianism consciousness among adolescents and to determine the relationship between the two factors. Method: Data were collected from 376 middle and high school students in U City. Tools used were the Korean Sexual Role Identity (Kim, J. H., 2005) and the Gender Egalitarianism Consciousness among Adolescents (Kim, 2002). Data were analyzed using SPSS 12.0 for t-test, one-way ANOVA and Pearson correlation coefficient. Results: There was no significant difference between boys and girls in sender role identity. Boys' gender role identity was different according to school grade (t=-3.19, p= .00), frequency of mother's hugging (t=-2.28, p= .02) and girls' according to religion (t=-2.24, p= .03) mother's job (F=3.59, p= .02), father's education level (F=3.20, p= .04), mother's education level (F=3.24, p= .04), family harmony (F=7.28, p= .00), and frequency of mother's hugging (t=-2.41. p= .02). There was significant difference in egalitarianism consciousness according to sender (t=-12.70. p= .00) Boys' egalitarianism consciousness was different according to school type (t= .02, p= .00) and father's education level (F=4.97, p= .02), and girls' according to school grade (t=-4.21 p= .00), school type (t=-3.12, p= .00), father's level of education (F=3.78. p= .02), decision maker of important domestic matter (F=4.95, p= .00) and frequency of mother's hugging (t=2.53, p= .01). In Girls, sexual role identity and sender egalitarianism consciousness were significantly correlated with each other (r= .01, p= .04). Conclusion: Through the study, it was found that boys' concern for sexual equality of was remarkably lower than girls. Therefore, it is necessary to offer curriculums for boys and to start social education for parents.

• Korean Journal of Adult Nursing
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• v.24 no.3
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• pp.294-304
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• 2012

Purpose: The purpose of this study was to examine the effects of taping therapy on the range of motion and pain of shoulders, physical functions and depression among hospitalized patients with stroke a geriatric hospital. Methods: The study design was a none equivalent control group pre-post test quasi-experimental design. The participants were 25 patients for experimental group and 25 patients for the control group. The period of this study was from March to July 2010. The twenty minute taping therapy was performed for twice a week for eight weeks. Results: The results showed that taping therapy was effective in range of motion shoulders (flexion: t=-5.81, p =.001, abduction: t=-3.69, p =.001) and pain of shoulders (rest: t=3.18, p = .003, movement: t=2.97, p =.005), grip (t=-3.22, p =.002) and balance (t=3.20, p =.002), and depression (t=3.24, p =.002). The variable of ADL (t=-1.99, p =.052) was not significant. Conclusion: The results of the taping therapy increased range of motion shoulders and grip and balance of physical functions, and decreased pain in shoulders and depression. The findings support that taping therapy can be used as a nursing intervention for stroke patients in practical nursing and communities.

• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.321-325
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• 1996

Mutants having altered levels and/or types of EPS in exopolysaccharide biosynthesis were isolated after NTG mutagenesis of Zoogloea ramigera 115SLR. Mutant candidates were classfied with five groups based on the observed characteristics for EPS biosynthesis pattern. The recombinant plasmids pLEX3BS and pLEX3BM were constructed from pEX3B which was previously isolated from genomic DNA of Z. ramigera 115SLR. Plasmid pLEX3BM with a 7.8 kb insert DNA contains an additional 1.8kb DNA fragment which is not present in pLEX3BS containing 13 kb insert DNA. Plasmid pLEX3BM was able to complement the mutation responsible for the changes in morphology of Z. ramigera 115SLR. However, the complementation of EPS negative mutant strains was not successful with pLEX3BM. Plasmid pLEX3BS on the other hand complemented the mutation responsible for the loss of EPS biosynthesis, resulting in the restoration of Z. ramigera 115SLR phenotype. But this plasmid was not able to complement the morphological mutant strain, Z. ramigera 115SLR.

• Journal of Environmental Science International
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• v.12 no.7
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• pp.689-695
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• 2003

The purpose of this study is to evaluate and to predict of eutrophication in lakes by using Vollenweider-OECD model and total phosphorus concentration and inflow rate which were measured in 1993∼2001. The results of study were as follows. The annual total phosphorus loading from the watershed was calculated to be 55∼195tP/yr at lake Soyang, 221∼466tP/yr at lake Taechong, 123∼278tP/yr at lake Andong, 57∼109tP/yr at lake Seomjin. These are discharged, far the most parts, from population and fishfarm facility. TP loading on the surface area at lake Soyang was 3.01gP/㎡/yr, 2.82gP/㎡/yr, 2.84gP/㎡/yr, 3.03gP/㎡/yr, 2.34gP/㎡/yr, 1.78gP/㎡/yr, 0.91gP/㎡/yr, 0.89gP/㎡/yr, 0.86gP/㎡/yr, lake Taechong was 6.71gP/㎡/yr, 7.25gP/㎡/yr, 7.24gP/㎡/yr, 6.53gP/㎡/yr, 6.50gP/㎡/yr, 7.06gP/㎡/yr, 7.04gP/㎡/yr, 4.05gP/㎡/yr, 3.44gP/㎡/yr and TP loading on the surface area of lake Andong, lake Soemjin were 5.39gP/㎡/yr, 4.47gP/㎡/yr, 4.56gP/㎡/yr, 4.45gP/㎡/yr, 3.33gP/㎡/yr, 2.38gP/㎡/yr, 2.53gP/㎡/yr, 2.46gP/㎡/yr, 2.54gP/㎡/yr, 4.09gP/㎡/yr, 4.10gP/㎡/yr, 3.98gP/㎡/yr, 3.73gP/㎡/yr, 2.80gP/㎡/yr, 3.46gP/㎡/yr, 3.22gP/㎡/yr, 2.19gP/㎡/yr, 2.13gP/㎡/yr respectively. The tropic states of four lakes can be assessed as eutrophy because phosphorus leading exceeds the critical phosphorus loading by Vollenweider-OECD model.

• Applied Biological Chemistry
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• v.36 no.5
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• pp.370-375
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• 1993

Five electrophoretic bands of crude enzyme extracted from rice leaves were found to possess both chitinase and ${\beta}-1,3-glucanase$ activities. These $chitinase/{\beta}-1,3-glucanase$ were resolved into acidic and basic fractions of protein by DEAE-cellulose and chitin affinity column chromatography. The optimal pH and temperature for ${\beta}-1,3-glucanase$ activity of two fractions were in the same extent as pH 5 and $60^{\circ}C$, whereas those for chitinase activity differed from one another; pH 3 and $60^{\circ}C$ for the acidic and pH 4 and $50^{\circ}C$ for the basic fraction, respectively. In addition, lysozyme activity was found in both fractions.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.625-636
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• 2005

This study was conducted to determine effects of different ratios (3.5, 3.0 and 2.5) of nonfibrous carbohydrate (NFC) to ruminally degradable protein (RDP) on in vitro fermentation and lactation performance of dairy cows and optimum ratio of NFC to RDP in dairy rations. In vitro trial was conducted up to 12 hr with ruminal fluidtaken from ruminally cannulated Holstein cows. The level of dietary NFC did not affect ruminal pH. The ammonia-N concentration was not significantly different among treatments until 6 hr incubation, however, it was significantly (P < 0.05) decreased as the ratio of dietary NFC to RDP increased on 9 and 12 hr incubation. For volatile fatty acids, concentrations of both acetate and propionate were significantly (P < 0.05) increased on 3 hr incubation as dietary NFC contents of treatments increased, in other incubation times, they had no significant differences among treatments. Valerate and A:P ratio were not affected by the ratio of NFC to RDP. Isoacids and total VFAs were significantly (P < 0.05) increased with increasing dietary NFC contents and their values were highest in the treatment of 3.0 ratio. Meanwhile, for in vivo trial, 18 Holstein lactating cows were allotted to treatments in three groups of 6 cows. They were employed for 24 weeks to investigate nutrient intakes, and milk yield and composition according to different ratios of dietary NFC to RDP. Intakes of dry matter and energy were significantly (P < 0.01) increased, but NDF intake was significantly (P < 0.01) decreased as the ratio of dietary NFC to RDP increased. Milk yield for the ratio of 3.5 (32.7 kg) was significantly (P < 0.05) higher than those of other treatments. Milk fat (%) was significantly (P < 0.05) higher for the treatments of 3.0 (3.79 %) and 2.5 (3.79 %) than that (3.48 %) for the ratio of 3.5, but milk fat yield was not different among treatments. Contents and yields for milk protein and solids-not fat were linearly (P < 0.01) increased as the ratio of dietary NFC to RDP increased. However, milk urea nitrogen concentration was significantly (P < 0.05) decreased with increasing dietary NFC levels. Our results showed that the increasing level of NFC in the diet of dairy cows enhanced ruminal fermentation, N utilization and milk production and suggested that maximal fermentation and lactation performance were achieved when the dietary ratio of NFC to RDP was more than 3.0 in dairy rations.