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Effects of Silkworm (Bombyx mori) Pupa Extract on the Function of Osteoblastic MC3T3-E1 Cells

  • Choi, Eun-Mi;Lee, Kyung-Hee;Koo, Sung-Ja
    • Food Science and Biotechnology
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    • 제14권5호
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    • pp.593-598
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    • 2005
  • Osteoporosis is recognized as one of the major hormonal deficiency diseases, especially in menopausal women and the elderly. When the estrogen level is reduced in the body, local factors, which are known to be related with bone resorption, are increased and promote osteoclastogenesis. In our previous study, we validated the estrogenicity of silkworm pupa. In this study, we investigated the effect of silkworm pupa extract (SPE) on the function of osteoblastic MC3T3-E1 cells. SPE (10 and $50\;{\mu}g/mL$) significantly elevated cell viability, alkaline phosphatase (ALP) activity, and collagen content in the cells. The effect of SPE ($50\;{\mu}g/mL$) in increasing cell viability, ALP activity, and collagen content was completely inhibited by the presence of $10^{-6}\;M$ of cycloheximide and $10^{-6}\;M$ of tamoxifen, suggesting that SPE's effect results from a newly synthesized, protein component and that it might be partly involved in estrogen action. Furthermore, we examined the effect of SPE on the $H_2O_2-induced$ apoptosis and production of local factors in osteoblasts. Treatment with SPE ($50\;{\mu}g/mL$) decreased the 0.2 mM $H_2O_2-induced$ apoptosis and the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6 and nitric oxide (NO) in osteoblasts. Our data indicate that the enhancement of osteoblast function by silkworm pupa may prevent osteoporosis and inflammatory bone diseases.

송화설기의 재료 배합비에 따른 관능적$\cdot$텍스쳐 특성 (Sensory and Mechanical Characteristics of Songhwasulgi by Different Ratio of Ingredients)

  • 이효지;김하정;차경희
    • 한국식품조리과학회지
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    • 제21권4호
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    • pp.505-513
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    • 2005
  • The purpose of this study was to investigate the product characteristics of 'Songhwasulgi,' which combines different kinds of pine pollen flours and sugars with non-glutinous, rich flour. The results of sensory evaluation indicated that the larger amount of pine pollen flour was mixed with non-glutinous rice flour higher level of bitterness, softness, and feeling after-swallowing was obtained degree of chewiness was in relation to the lesser amount of pine pollen flour. With regard to color, a higher acceptance level was achieved as the lesser amount of pine pollen flour was incorporated with non-glutinous rice How. On the other hand, it was revealed that the category of adding a honey to $7\%$ of pine pollen flour was found to be the highest level of flavor and overall quality According to the results of texture evaluation, the degree of hardness, adhesiveness, and cohesiveness decreased as the larger amount of pine pollen flour was combined with non-glutinous rice flour. It was also found that the springiness of the texture tended to be low as more pine pollen flour was added. In addition, the degrees of gumminess and chewiness was inversely related to the amount of pine pollen flour With respect to a correlation between sensory evaluation and mechanical test, it was found that Songhwasulgi with higher acceptance level for color and flavor also obtained a higher level of hardness and overall quality.

연속 옴가열 장치를 이용한 고추장 소스의 살균 (Sterilization of Gochujang Sauce with Continuous Ohmic Hea)

  • 최준봉;조원일;정정윤;정명수
    • 한국식품과학회지
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    • 제47권4호
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    • pp.474-479
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    • 2015
  • 연속식 옴가열 장치를 이용한 고추장을 베이스로 한 모델 식품에 대한 가열 살균 연구를 진행하여, 옴가열을 이용한 소스 살균에 필요한 기초 데이터를 수집하였다. 옴가열 시 점도에 상관없이 $100^{\circ}C$까지 고르게 상승하였으나, 점도가 높을수록 전기전도도와 가열속도가 낮아져 살균력이 감소하는 것으로 나타나, 저점도 식품이 옴가열을 이용한 가열 및 살균에 있어 더 유용하다는 것을 알 수 있었다(13,14). 재래가열 후의 살균도는 65.4-75.4%, 옴가열 시는 89.5-95.0%로 살균력이 더 우수하였다. 향후 연속식 옴가열 장치의 살균효과를 더 높이기 위해서는 여러 개의 셀 연결 방식을 통해 한 개의 셀에서 온도를 조금씩 상승시켜 스케일 생성을 방지하는 연구와 셀 통과시 빠른 온도 상승에 의한 점도 변화로 살균 처리량이 변하기 때문에 최적 셀 조합 및 가열살균 조건에 대한 충분한 고찰이 필요할 것으로 생각된다.

노인소비자의 학력수준이 외식구매의사결정 과정에 미치는 영향에 관한 연구 (Effect of the Elderly Consumers' Education Level on Eating-Out Decision Making Process)

  • 김태희;서은
    • 한국식생활문화학회지
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    • 제20권6호
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    • pp.638-643
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    • 2005
  • As Korea has approached the aging society, older Koreans have become an important force in restaurant sales today. To succeed with this silver market, it is important for restaurant managers to know who they are and what factors influence older Koreans' eating-out decision making process. The purpose of this study was to investigate the effect of the silver consumers' education level on eating-out decision making process. Data were collected from 178 older consumers above 55 years old and analyzed using the descriptive statistic analysis, MANOVA, and one-way ANOVA. The results showed that the elderly consumers' education level significantly influenced the decision making process in determining where to eat out. Significant differences were found in the Problem Recognition Step(Wilks' Lambda=0.817, F=2.991), Information Search Step(Wilks' Lambda=0828, F=2.218), Alternative Evaluation Step II(Wilks' Lambda=0.741, F=3.596), Purchase Decision Step(Wilks' Lambda=0.859, F=2.223), and the Post-Purchase Behavior(Wilks' Lambda=0.885, F=1.780). The higher education level was, the more directly involved in the eating out decision process. The elderly consumers with university education were likely to 'propose to eat out by themselves'(F=9.346), to obtain restaurant information from the 'printed materials'(F=7.452), to go to 'family restaurant'(F=9.057), 'Japanese restaurant'(F=8.7891) and 'fine dining restaurants'(F=3.936), and to directly express their emotion when they had complaints about restaurant service(F=3.206). In conclusion, older Koreans will become more healthy and wealthy which means the dining out activity will be an important part of their life to socialize with people. Therefore, food service operations should consider the elderly consumers' needs and expectation of restaurant services and actively position themselves for this new market segment.

부산 지역 전통음식에 대한 세대별 인식 차이 분석 (A Study on the Difference of Perception about Traditional Food by Generations in Busan Area)

  • 이복섭;박헌진;정진우
    • 한국조리학회지
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    • 제16권4호
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    • pp.1-14
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    • 2010
  • 본 연구는 부산 지역에 거주하는 사람들의 한국음식에 대한 세대별 차이를 분석하였다. 조사 대상은 부산 지역에 소재하는 중학생, 고등학교, 대학교, 일반인들을 대상으로 표본을 선정하여 2008년 8월 1일부터 30일에 걸쳐 실시하였으며, 분석 방법은 빈도분석, 교차분석을 실시하였다. 연구 결과는 다음과 같다. 첫째, 세대별로 전통음식을 선호하는 빈도가 차이가 있다는 것을 찾을 수 있다. 나이가 많을수록 전통음식을 좋아하였으며, 반대로 젊은 층에서 전통음식을 좋지 않게 생각하는 경향이 있는 것을 알 수 있다. 둘째, 모든 세대에서 가장 좋아하는 식사 형태는 한식이며, 셋째, 가정에서 식사를 준비할 때 아버지를 위주로 하여 식사를 준비하는 것으로 나타났다. 넷째, 음식을 만드는 방법에는 바쁜 현대인들에게는 좀 더 단순화시키고 표준화하여 조리하기 쉽게 하는 조리법이 필요하다. 부산 지역 한국음식의 세대별 차이의 개선점으로는 청소년을 대상으로 한 전통음식의 맛 개발에서 전통음식은 발효음식의 냄새는 허브나 조미료를 첨가하여 모던하게 만들어야 할 것이고, 또한 전통적인 방식도 식품의 재료를 현대식으로 교체와 조리 방법을 변화를 주어 어릴 때부터 자연스럽게 접할 수 있도록 하여야 한다. 향후의 연구에서는 조사 대상자를 전국적으로 확대하여 지역별에 따른 차이를 분석하여 좀 더 균형 있는 표본의 선정을 통한 보완이 필요하리라 본다.

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마늘설기의 재료 배합비에 따른 관능적$\cdot$텍스쳐 특성 (Sensory and Mechanical Characteristics of Maneul-Sulgi by Different Ratio of Ingredient)

  • 이효지;이은선;차경희
    • 한국식품조리과학회지
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    • 제21권2호
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    • pp.180-189
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    • 2005
  • The purpose of this study is to determine the optimal mix ratio of Maneul-Sulgi and thereby, review its availability as health cake. For this purpose, the ratios of garlic juice and powder were varied with sugar added. As a result of quantitative descriptive analysis, it was found that the more the garlic juice and powder were added to non-glutinous rice, the Garlic taste was stronger. On the other hand, the less garlic powder was added, the cake would taste softer, and the less garlic juice was added, the cake was more chewy and moist. The feeling after swallowing was best when the ratio of garlic juice was $7\%$. As a consequence of surveying the tastes of Maneul-Sulgi, it was found that the less garlic powder was added to non-glutinous rice, the cake was more preferred. It was perceived that the ratio of garlic juice should be $7\%$ for flavor and desirable taste. As a result of testing the mechanic characteristics, it was found that the less garlic juice was used, the cake was more hard, elastic, cohesive, viscose and chewy. The more garlic powder was used, the Adhesiveness was higher. The Overall acceptability of sensory examination for Garlic taste had positive correlation Hardness of sensory examination and mechanical examination for adhesiveness.

Cytokine modulation in Raw 264.7 macrophages treated with ginseng fermented by Penibacillus MBT213

  • Son, Ji Yoon;Renchinkhand, Gereltuya;Bae, Hyoung Churl;Paik, Seung-Hee;Lee, Jo Yoon;Nam, Myoung Soo
    • 농업과학연구
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    • 제45권4호
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    • pp.769-777
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    • 2018
  • The fermentation of Panax ginseng yields many compounds including ginsenosides that have various biological functions. The objective of this study was to investigate the modulation of nitric oxide (NO), Interleukin (IL)-6 and tumor necrosis factor $(TNF)-{\alpha}$ in Raw 264.7 cells treated with ginseng fermented by Penibacillus MBT213. Nitric oxide production in the Raw 264.7 cells treated for 24 hours with fermented ginseng at 3, 7, and 14 days after the treatment decreased to 74, 43, and 36%, respectively, compared with the positive control. The production of IL-6 was inhibited in all the cells treated with fermented ginseng at 3, 7, and 14 days after the treatment except for the positive control. The $TNF-{\alpha}$ production in the Raw 264.7 cells treated with fermented ginseng for 6 hours at 3, 7, and 14 days after the treatment was about 40,000, 85,000 and 65,000 pg/mL, respectively. Moreover, the $TNF-{\alpha}$ production in the Raw 264.7 cells treated with fermented ginseng for 24 hours at 7 and 14 days after the treatment was about 160,000 and 180,000 pg/mL, respectively. However, $TNF-{\alpha}$ production was inhibited in the Raw 264.7 cells at 6 and 12 hours after the treatment with fermented ginseng. herefore, it was confirmed that the immunological activity of the Raw 264.7 macrophages was affected by the treatment with fermented ginseng. It was concluded that ginseng fermented by Paenibacillus MBT213 possesses a potential anti-inflammatory activity and could be used as an ingredient in functional foods and pharmaceutical products.

지역 임산물의 공유가치창출에 대한 소비자의 인식에 따른 지역 임산물의 구매행동에 관한 연구 - 정읍시를 중심으로 - (Effect of Consumers' Perceived Creating Shared Value on Purchasing Behavior of Non-timber Forest Products in Jeongeup City, Korea)

  • 오지은;이경원;강남이;조미숙;나예슬
    • 한국식생활문화학회지
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    • 제36권5호
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    • pp.421-429
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    • 2021
  • Local non-timber forest products can be easily accessed by tourists. These also provide opportunities for tourists to experience the local culture. Many local governments are paying constant attention to the development of non-timber forest product brands to revitalize the local economy and increase the profits of local farmers. Although there are many forest products and tourism resources in each region of Korea, there is no local specialized strategy to sell non-timber forest products and promote consumers' purchase intentions. In this study, we aimed to explore the consumers' perceived creating shared value (CSV) and its association with purchasing behavior of non-timber forest products in Jeongeup city, Korea. Data were collected from 1,066 consumers who had purchased non-timber forest products. Purchasing patterns of local specialty products differed depending on consumers' perceived CSV levels. In addition, consumers with higher levels of perceived CSV showed higher awareness and familiarity with Jeongeup city and experienced Jeongeup city's non-timber forest products. Our findings suggest that improving consumer perception towards CSV of non-timber forest products can be used as a marketing strategy for local non-timber forest products to revitalize the local economy.

에스닉 푸드점 외식업의 실태와 서비스 품질이 서비스 실패, 회복, 신뢰, 만족, 충성도의 상관관계에 관한 연구 (The domestic situation of ethnic food restaurants and Service Failure, recovery, trust, satisfaction, loyalty of the Study on the Quality of Service)

  • 박지수
    • 한국산학기술학회논문지
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    • 제22권6호
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    • pp.385-397
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    • 2021
  • 본 연구는 국내 에스닉 푸드점 외식업의 실태와 서비스 실패, 회복, 외식 서비스품질이 고객만족과 재이용의도에 미치는 영향을 연구하고자 하였다. 본 연구는 에스닉 푸드점의 서비스 실패의 심각성과 통제성뿐만 아니라 서비스 품질의 관계의질, 즉 신뢰, 만족 및 충성도에 관련한 변수의 인과관계를 규명하여 서비스 품질에 대한 모형을 확장하였다는데 의의를 두었다. 그 결과, 서비스 실패를 경험하고 실패 상황의 심각성과 통제성을 인지한 고객에게 분배적 공정성을 최우선적으로 제시할 수 있는 전략을 수립하는 것이 중요하다는 것을 알 수 있다. 또한 본 연구가 현재 국내에서 운영하고 있는 외식업체를 대상으로 조사를 실시함으로써, 우리나라 외식관련업체 관리자들에게 실무적으로 도움이 될 수 있는 방안을 고려하여 외식업 서비스 실패 및 그 회복에 관한 유용한 정보를 제공, 보다 체계적인 사업계획을 수립, 고객관리의 실천과 이에 따른 이익창출에 도움이 될 것으로 사료된다. 현재 본 연구가 가지는 이점으로 불경기의 현 외식시장을 극복하고 더욱 성장하고 굳건히 자리 잡기 위해서 국내 외식업 호황과 서비스품질에 관한 실질적인 연구 자료가 필요한 시점이다.

컨조인트 분석을 적용한 영국 소비자 집단의 매운 소스 선호도 조사 (Assessing Relative Preference for Hot/Spicy Sauces by Conjoint Analysis, Focusing on English Consumers)

  • 임성일;한경수;;김재호;서경미
    • 한국식생활문화학회지
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    • 제24권1호
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    • pp.51-57
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    • 2009
  • The purpose of the study was to assess the relative preference for hot & spicy sauces using conjoint analysis, with focus on English consumers. From the results of the study, we were able to derive a standard with the best sauce attributes. The respondent group was selected from the CCFRA's customer database. The qualification criteria for inclusion in the sample were: the primary grocery shopper in the household, a consumer of a range of home cooked oriental & far eastern cuisines, enjoyed hot & spicy chili-based foods, and willing to buy hot chili-based oriental sauces. A total of 676 respondents completed the survey in which 76% were women, and all respondents were between the ages of 18 and 65 years. An online survey method was used and a conjoint analysis was adopted. In conjoint analysis, a product is described as a combination of a set of attribute levels, where a utility value is estimated for each attribute level. In summing up the results of this study, the sensory property (flavor) attribute was most important, the second was brand, and the third was price. For the sensory property attribute, a mild chili sauce of blended garlic, sugar, and lime scored highest. In terms of brand, Blue Dragon was selected as the best. The lesser known Korean Kochujang brand of "Hot&Joy" scored lowest with respect to brand value. Encouraging, however, was the description based on the Hot&Joy product: a hot chili sauce blended with ginger and garlic, which held appeal across age groups and genders. In terms of price, 75p per bottle had the best score.