• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 1994.03a
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• pp.35-49
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• 1994

Based on the safety of irradiated foods which was demonstrated from the toxicological, microbiological and nutritional points of view, irradiation has been identified as a viable technology for food preservation and processing, having a potential both of reducing storage losses by controlling spoilage organisms, sprouting and ripening, and of improving hygienic quality of raw and processed products. Research and development over decades in the field of food irradiation have led to the regulatory approval in 37 countries and of them 25 countries including Korea are commercially utilizing food irradiation process. Although progress towards acceptance of food irradiation by the industry is slow, actual market trials have shown that once consumers have understood this technology, they are willing to buy irradiated foods. Considering recent advances in food irradiation and restrictions in the use of chemical fumigants, it is expected that food irradiation is in the process of "taking off". This paper deals with up-to-date progress in food irradiation with particular reference to domestic activities in legislation, consumer perception, commercialization, and potential applications in the food industry.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.5
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• pp.692-700
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• 2012

The antibacterial activity of the ethanol extract of Cornus officinalis against some bacteria related to foodborne illness and food spoilage was investigated. In the case of the disc diffusion assay, clear zones due to the inhibition of proliferation by Cornus officinalis extract were 8.5~18.3 mm at $4,000{\mu}g/disc$. The inhibitory effect of Cornus officinalis was as follows: Escherichia. coli ${\geq}$ Listeria monocytogenes ${\geq}$ E. coli O157:H7 > Bacillus cereus > Staphylococcus aureus > Pseudomonas aeruginosa > Salmonella typhimurium. In addition, the MIC of Cornus officinalis extract toward B. cereus was the lowest at 1,000 ppm. The extract inhibited the growth of E. coli, L. monocytogenes, E. coli O157:H7, and Bacillus cereus throughout the growth stage. However, the growth of P. aeruginosa, S. aureus, and S. typhimurium was only inhibited by the extract during the lag phase. The constituents of all cells tested were released, especially E. coli and E. coli O157:H7. Observation of the cells using SEM demonstrated a morphological change and disruption of cells in response to treatment with Cornus officinalis extract. Based on these findings, the ethanol extracts of Cornus officinalis showed strong antimicrobial activity against all tested bacteria, indicating that Cornus officinalis can be a useful natural antimicrobial reagent.

• Korean Journal of Plant Resources
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• v.23 no.5
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• pp.430-435
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• 2010

This study was performed to investigate the antimicrobial effects of Ampelopsis brevipedunculata ethanol-extract on food spoilage or foodborne disease microorganism. The antimicrobial activity of Ampelopsis brevipedunculata extract was determined by a paper disc method. The extract exhibited growth inhibiting activities in a concentration dependent manner on 7 species of microorganisms. The extract of Ampelopsis brevipedunculata showed the highest antimicrobial activity against Escherichia coli O157:H7. The growth inhibitory effects of the extract at the concentration of 250, 500, 1,000, 2,000 mg/L on food poisoning microorganism were determined against Salmonella typhimurium, Yersinia enterocolitica, Escherichia coli O157:H7 and Staphylococcus aureus. The growth of microorganisms was not affected by the extract at the concentration up to 250 mg/L, but was significantly (p<0.05) inhibited by the extract at the concentration higher than 500 mg/L. The results in the present study demonstrate antimicrobial effects of Ampelopsis brevipedunculata ethanol-extract against foodborne pathogens, suggesting that Ampelopsis brevipedunculata could be an effective natural antibacterial agent in food.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.5
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• pp.997-1002
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• 1999

The aim of the present study was to investigate effects of food preservative addition and changes in composition of herb extract product on the growth of spoilage yeast, Zygosaccharomyces sp. Herbs such as Panax ginseng, Cinnamomum cassia, Lycium chinense, Zyzyphus juiuba and Jingiber officinale were altogether put into water and essence was extracted at 80oC, and then the extract was concentrated at 75oC. The herb extract product was made by adding vitamins, amino acids and honey to the concentrated herb extract. The amount of gas produced from the herb extract product was increased as inoculated cell number increased but decreased as Brix concentration increased. Gases were produced in small amount when incubation was made at 4oC but large amounts of gases were produced at 25 or 40oC of incubation. The gas production and growth of Zygosaccharomyces sp. were measured after browning reaction was induced by heating at 85oC for 12 hours. It appeared that heating treatment did not induce any significant change in the gas production and growth of the cell. The effects of addition of various sugar to the herb extract produce were also invesigated. Amounts of gas production were in the order of glucose>sucrose>oligosaccharide>stevioside. The viable cell count was measured as 6.0$\times$107 CFU/g when glucose was added to the herb extract product. The viable cell counts were 5.0$\times$106, 3.0$\times$103, and 3.0$\times$102 CFU/g in sucrose, oligosaccharide and stevioside added herb extract product, respectively. The amount of gas production from the herb extract product was remarkably reduced by addition of such food preservatives as sodium benzoate and DF 100. TLC(thin layer chromatography) chromatogram of the herb extract showed stability of the herb extract in the above treatments.

• Preventive Nutrition and Food Science
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• v.9 no.4
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• pp.306-311
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• 2004

This study was performed to investigate the antimicrobial effects of Portulaca oleracea extracts against food-borne pathogens. First, the Portulaca oleracea was extracted with methanol at room temperature, and then further fractionated by using petroleum ether, chloroform, ethyl acetate and methanol, respectively. The antimicrobial activity of the Portulaca oleracea extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of Portulaca oleracea showed the highest antimicrobial activity against Staphylococcus aureus and Shigella dysenteriae. There was also a synergistic effect of the combined extracts of Portulaca oleracea and Indigofera kirilowii as compared to each extract alone. Finally, the growth inhibition curve of ethyl acetate extracts of Portulaca oleracea against Staph­ylococcus aureus and Shigella dysenteriae was determined The ethyl acetate extract of Portulaca oleracea showed strong antimicrobial activity against Staphylococcus aureus at the concentration of 4,000 ppm. The 4,000 ppm of ethyl acetate extract from Portulaca oleracea, retarded the growth of S. aureus by more than 24 hand Shigella dysenteriae up to 12 h at $37^{\circ}C$.

• Food Science and Biotechnology
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• v.14 no.3
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• pp.311-316
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• 2005

This study was performed to investigate the antimicrobial effect of Sophora angustifolia extracts against food-borne pathogens. First, Sophora angustifolia was extracted with methanol at room temperature, and the methanol extracts from Sophora angustifolia were fractionated using petroleum ether, chloroform, ethyl acetate, and methanol. The antimicrobial activity of the Sophora angustifolia extracts was determined using the paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of Sophora angustifolia showed the highest antimicrobial activity against Staphylococcus aureus and Salmonella typhimurium. A synergistic effect was found in the combined extracts of Sophora angustifolia and Portulaca oleracea, compared to the activity of each extract alone. Finally, the growth inhibition curve was determined using the methanol extracts of Sophora angustifolia against Staphylococcus aureus and Salmonella typhimurium. The methanol extract of Sophora angustifolia showed strong antimicrobial activity against Staphylococcus aureus at a concentration of 5,000 ppm. The 5,000 ppm methanol extract from Sophora angustifolia retarded the growth of S. aureus for more than 24 hours and of Salmonella typhimurium for up to 12 hours.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.471-476
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• 1998

Microbiological spoilage of marine fish is complex process occurring by bacteria, yeasts and molds. There have been rare study for saprophytic yeasts although having enormous numbers of bacteriological studies on the spoilage of marine fish. The 14 genera of yeasts isolated from mackerel (Scomber japonicus) with high frequency of occurrence were Candida sp., Rhodotorula sp., Torulopsis sp., Cryptotoccus sp. and Tricosporon sp. Among these ones Candida lipolytica was identified as the strongest proteolytic yeast, then named Candida lipolytica FM5 (C. lipolytica FM5). C. lipolytica FM5 showed optimum growth at $25^{\circ}C$, pH 7.0 and could grow at $5^{\circ}C$ and in medium containing $10\%$ sodium chloride, To evaluate the saprophytic activity of the selected strain, C, lipolytica FM5 and Pseudomonas fluorescens ATCC 17571 which is one of representative spoilage bacteria were individually inoculated into the sterilized fish muscle homogenates, and then pH changes and volatile basic nitrogen (VBN) values were checked during the storage at various temperatures. According to the experimental results, the productions of VBN by C. lipolytica FM5 in the fish muscle homogenates were 50 mg-N/100 g at $5^{\circ}C$, 152 mg-N/100 g at $15^{\circ}C$ and 379 mg-N/100 g at $25^{\circ}C$ for 1 week storage, respectively. Above results were nearly same as in case of Ps. fluorescens ATCC 17571 inoculation. It suggest that sapyophytic yeasts also have important role in spoilage of marine fish.

• Korean journal of food and cookery science
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• v.17 no.6
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• pp.565-570
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• 2001

Soibirhym(Portulace oleracea) was extracted by methanol and its antimicrobial activities against food spoilage or foodborne disease microorganisms were investigated by the paper disc method. The microorganisms used in this experiment included 5 species of bacteria(Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella Pneumoniae, Staphylococcus aureus) and 3 species of fungi(Fusarium solani, Aspergillus flavius, Penicillium citreonigrum). Soibirhym showed high antimicrobial activites against P. citreonigrum, P. aeruginosa and K. pneumoniae. Minimum inhibitory concentrations(MICs) on S. aureus, P. citreonigrum and K. pneumoniae were 200, 200 and 250 mg/㎖, respectively. In the methanol extracts from Soibirhym, 147 kinds of compound were separated by GC/MS. The extraction yields of phenolics, furans, alcohols, acids and esters, ketones, aldehydes, and miscellaneous compounds were 7.43%, 6.13%, 2.20%, 41.06%, 9.21%, 0.15% and 1.08%, respectively. Some antimicrobial compounds such as 2,3-dihydro-benzofuran, 4-hydroxy-3-methoxy-benzoic acid, 4-hydroxy benzeneethanol were detected in the methanol extract.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.1
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• pp.111-116
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• 2007

We examined the antimicrobial activity of electrolyzed alkaline water against spoilage microorganisms in rice warehouses. Dominant microbial species were isolated from paddy, dust and air samples of three different warehouses located in Gyeongnam. We used electrolyzed alkaline water (EW) manufactured over various treatment times. The acidities (pH) of EW treated for 30 sec, 1, 2, 3, and 4 min were 8.89, 8.91, 9.20, 9.35, and 9.22, respectively. HClO contents were 150.7, 314.2, 191.8, 104.1, and 255.3 ppm, respectively. EW inhibited bacteria, yeasts, and molds; also, it inhibited three yeast species strongly. The antimicrobial effects of EW increased as pH values and HClO contents increased. EW obtained after 30 sec treatment retained the antimicrobial activities after 14 days whereas EW obtained after 1 and 2 min treatments retained the antimicrobial activities after 21 and 24 days, respectively.

• Food Science and Biotechnology
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• v.18 no.2
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• pp.425-431
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• 2009

Antimicrobial coating on chicken carcasses may reduce the effects of cross-contamination and improve product shelf-life and safety. Thyme oil was mixed at 0.5%(v/v) with a pre-gelatinized pea starch coating solution. The coating solution was spread on chicken breast meat after inoculation with selected spoilage and pathogenic bacteria. After inoculation, the chicken meats were packaged in plastic bags and stored at $4^{\circ}C$. During 12 day storage, total aerobic bacteria, lactic acid bacteria, and inoculated organisms were counted at 4 day intervals. Thyme oil treatments reduced the viability of Salmonella as well as the growth of Listeria and Pseudomonas by 2 log CFU/g, and appeared to eliminate inoculated Campylobacter during storage. The addition of thyme oil increased the viscosity of the pre-gelatinized pea starch solution. The results suggested that thyme oil inclusion in an edible starch coating may be a satisfactory delivery system to enhance the safety of processed fresh meat.