• The Korean Journal of Community Living Science
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• v.26 no.2
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• pp.287-301
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• 2015

This study provides basic data for the development of nutrition education programs for high schools by examining adolescents' demand for nutrition education and their dietary and health behaviors. A survey was conducted for 500 students attending two high schools in the Gwangmyeong area. The questionnaire addressed their dietary habits, health behaviors, and nutrition education. In terms of meal conditions, 70.6% of all respondents had meals at least four times a day, 67.2% skipped breakfast frequently, and only 29.9% had breakfast with their family members. In addition, 66.0% reported a slightly unbalanced diet depending on the food (35.1% for vegetables and 28.2% for legumes). Nutrition labeling was checked occasionally (44.4%) by male respondents and surely (39.3%) by female respondents (p<0.01). In terms of health behaviors, 67.4% of male respondents reported a lack of sleep (5-7 hours), 33.3% of male respondents reported exercising three to four times a week, and 42.7% of female respondents reported no exercise. For the level of stress, 58.9% of male respondents reported "sometimes", and 43.0% of female respondents reported "always". For interest in nutrition education, 58.3% of the respondents reported "no". A majority of the respondents learned about nutrition through "the media or the Internet" (89.6%). The health behavior score was significantly carelated with dietary habits (p<0.01) and the stress score (p<0.05) for male students, and it was negatively correlated with checking nutrition labels (p<0.01) and positively correlated with the BMI for female students. These results suggest that appropriate health behaviors can help overcome stress and produce better dietary habits for male students, but for females, the results are ambiguous. A majority of the respondents (57.9%) perceived a need for nutrition education as part of the high school curriculum, and the content of such a curriculum included more meals with family members and the importance of breakfast. Based on the results, nutrition education for male students should focus on nutrition labeling, whereas that for female students, on exercise frequency and proper weight management.

• Journal of Society of Preventive Korean Medicine
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• v.1 no.1
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• pp.149-165
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• 1997

In order that, investigating the feature of patients suffering arthritis, analysing its contents, and grasping a Primary factor affecting it, I might offerbasic datas which could help to plan and perform healthy affairs to thake precautions beforehand, I have investigated, analysed, and studied a total of 320 patients suffering arthritis, who have received physiotherapy in hospital located in Teaegu area for five months, from November 1 1995 to March 30 1996, of which summary and conclusion is this. 1. The general feature of patients in investigative objects In the distribution of the distinction of sex, men accounted for 26.9% and women, for 73.1%, and, in the fistribution of age, 60-year-old or more, most for 27.2% and from 20 to 29 years old, least for 14.0%. In the distinction of a vocation, housewives most accounted for 34.7% and students(jobless men), least for 19.3%. In the distinction of a matrimonial state, married persons most accounted for 76.7% and people living alone(divorce, separation by death, separation), least for 11.4%. In the distinction of an economic state, the middle classes most accounted for 73.5% and the upper classes, least for 2.9%. In the distinction of their academic careers, graduates of a primary school most accounted for 26.9% and graduates of university, for 14.1%, of which patients, having the ability to decode the national language, reached to 11.3%. In the distinction of the house form, people living in independent houses most accounted for 76.4% and residents in apartment(having an elevator), least for 9.4%. 2. In the distribution of the recurring state in the distinction of the feature, the recurring group was more than the group of patients falling that ill at first as 62.2% and in the distinction of the feature of the recurring group, the recurring group turned high in case of men being from 50s to 60s years old or more, people living alone (divorce, separation by death, separation), students (joblessmen), people working in farming, stockbeeding, forestry, fisheries, a simple labour, graduates of a primary school I having the ability to decode the national language, the upper classes, people part two years since they begined to suffer arthritis, people who had members having ever experienced arthritis among families. 3. In the distribution of arthritis on the distinction of bodily pars, a knee articulation most accounted for 50.2% and the articulation of fingers, for 8.8%, wile the simultaneous, several parts (multiple) accounted for 35.1%. In the distinction of the feature, arthritis of a knee turned high in case of men being from 20s to 30s years old, unmarried persons, people having academic careers of university, the middle classes, residents in apartment (having stairs). In the dictnction of a feature the case of several parts (multiple) turned high in case of women being from 50s to 60s years old or more, people living alone (divorce, separation by death, separation), people having the ability to decode th. national language, the graduates of a primary school, the upper classes, residents in apartment (having elevator). 4. In the distribution of arthritis on e distinction of a contracting term, two years or more most accounted for 51.6% and the case of contacting from one year to two years, for 15.3%. Analysing the distinction of the feature, the case of two years or more turned high in case of women being from 50s to 60s years old or more, people living alone (divorce separation by death, separation), the upper classes, people having the ability to decode the national language, residents in apartment (having elevator). 5. In the distribution of an treatment institution before patients came to help, their not curing most accounted for 39.1%, general, orthopedic, neurological surgery (physical therapy), for. 20.0%, and th. therapy of Chinese medicine (acupuncture, moxacautery, Chinese medicine), for 17.5%, and a pharmacy (medical therapy), for 13.4%. The case of patients not curing, in the distinction of a feature, turned high in case of men 20s years old, unmarried, the lower classes, people having academic careers of university, residents in apartment (having elevator). 6. In e distribution of the extent of satisfaction with treatment, common most accounted for 54.4% and some satisfaction, for 32.8%. The case of common, in the distinction of a feature, turned high, in case of men living alone from 50s to 60s years old (divorce, separation by death, separation), married persons, the upper classes, people having academic careers of university, residents in independent house, residents in apartment (having elevator), 7. In the distribution of the degree of knowledge of the cause of arthritis, patients knowing that the cause is to use very much a articulation in normal times most accounts for 60.1%, and patients knowing the state of short nutrition as a cause, for 2.5%. The case of patients knowing that the cause is to use very much in normal times, in the distinction of a feature, turned high in·case of ment being 20s and 60s years old or more, unmarried persons, e lower classes, people having the ability to decode. the national language, people having academic careers of university, residents in apartment (having stairs), 8. In the distribution of the state of physical exercise before arthritis contracted, patients exercising very much on the whole most accpimend for 40.3%, and patients not exercising, for 34.7%. The case of patients exercising very much on the whole, in the distinction of the feature, turned high in case of men being from 50s to 60s years old or more, people living alone(divorce, separation by death, separation), the lower classes, people having the ability to decode the national language, graduates of a primary school, residents in apartment (having elevator). 9. In the taste of patients suffering from arthritis, while the group of patients falling that ill at first and the recurring group didn't smoke cigarets, during alcohol and coffee on the whole, and the group of patients falling once again that ill drank a cup of distilled linquor and three cup of coffee or more on the whole per one day, and the group of patients falling that ill at first liked sort of vegetables and the recurring group liked very much sons of vegetables and fresh and meat in their loving food normal times. 10. Analysing the distribution on the dining table used by patients and the structure of a powder room, at first, in the structure of a powder room, the group of patients filling that ill have a toilet stool using as their sits, and a Bush toilet on the whole, and the recurring group, a toilet stool using as their sits and conventional type, and in the structure of a dinning table, the group of patients falling that ill at first and the recurring group turned high, each as 66.9% and 6.3%, who have a dining table carring here and there. 11. In the distribution of patients of arthritis in relation to stress, the case that they feeled severly symptoms of arthritis when thay got stress, turned high, each, as 78.6% in the recurring poop, and the case not knowing, as 61.5% in the first group. In the extent of stress normal times, the case that they got much stress on the whole turned high, each, as 72.4% in e recurring group, and the care that got less stress on the whole, as 60.0%. 12. In the distribution on the distinction of symptoms and impedimental extent, the recurring group turned high in each variable. Analysing the feature of the recurring group, in the distinction of symptoms, the case that they fooled much that the node of an articulation is stiff, turned high, as 71.6, and in the distinction of treatment before. patients came to helpk, the theraphy of Chinese medicine (physical theraphy), as 84.4%, the theraphy of Chinese medicine (acupuncture, moxacautery, Chinese medicine), as 73.2%, and in the distinction of the satisfing extent on treatment, the case of comman, as 72.3%, and in the cause of arthritis, the case not recruiting their health after a birth, as 68.5%, and the case not recovering wholely an articulation having got hurt, as 62.8%, and in the state of physical exercise before they begined suffering from arthritis, the case exercising very much on the whole, (as 74.2%), and in the extent of subjective impediment, the case of not being able to act almost, as 66.7%, the case of acting but feeling some hard, as 66.3%. 13. The correlation in variables in relation to arthritis Analysing realted variables, the recurring frequency showed correlation with such as the extent that patients got stress normal times, and the exercising state before suffering arthritis, and showed contra-correlation with academic careers, the wights, coffee. The cigaret, e loving food of taste, showed corralation with the weight, stature, alcohole as the loving food of taste. On the basis of this result medical members of heal, who are related to the regular education, public education or development of this program, should be concerned to prevent orthris.

• The Korean Journal of Community Living Science
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• v.17 no.4
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• pp.151-161
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• 2006

This study was made to find out how health practice affect on nutrition status of the college freshmen in male and female. 400 freshmen students in 4year colleges were surveyed. Questionnaire and blood analysis were made. The contents of questionnaire contained general characteristics, health practice behavior, and food intake. The physical measurement included height, weight and blood pressure. The questions for health practice were about drinking, smoking, BMI, skipping breakfast and sleeping. Nutrient intake was expressed by DDS(Dietary Diversity Score by 5 food groups) and DVS (Dietary Variety Score) and 24-hrs recall method was used to find out the quantity of daily food intake. EAR% was calculated and NAR and MAR were produced. The results are as follows: 1) BMI was $22.77kg/m^2$ for males and $20.42kg/m^2$ for females. The ratio maintaining normal weight indicated by BMI of 18.5-23 was higher(p<0.001) for female students(63.2%) than males(56.5%). The ratio of overweight with over $23kg/m^2$ of BMI was 28.5% for males and 8.8% for females(p<0.001). 2)The ratio of drinking students was 59.4% for males and 40.6% for female(p<0.001). And the ratio of smoking students was 95.2% for males and 4.8% for females(p<0.001). 3) The ratio of poor health practice group was 35.7% for males and 25.5% for females, and that of excellent one was 25.4% for males and 33.1% for females(p<0.05). 4) In NAR, the lowest ratio was observed for the intake of Ca, Fe, Vitamin C and Vitamin $B_2$. The intake of Fe, Vitamin $B_2$ and phosphorus were different significantly according to sexuality. For Fe, female students showed lower level than male students(p<0.001). For Vitamin $B_2$, and phosphorus female students showed higher level(p<0.001). 5) In MAR, there was no difference according to sexuality in almost all of the ingredients. 6) For energy intake, male and female students showed 59% and 66% of EAR and it was insufficient for them. 7) For protein, more than 100% was obtained. The lowest ratio was observed for the intake of vitamin C 50.8%, Ca 53.6% and Fe 65.1% of EAR were obtained. The lowest intake ratio and for the intake of Fe female students obtained just 37.5% of EAR showing high risk of anemia. The intake of energy(p<0.001), phosphorus(p<0.01), niacin(p<0.05), Fe(p<0.001), and Vitamin $B_2$ (p<0.001) were different significantly according to sexuality. For Fe, female students showed lower level than male students(p<0.001). For energy, phosphorus, niacin, and Vitamin $B_2$ female students showed higher level(p<0.001) than male students. 8) For blood pressure, male students showed normal level of 120.7/79.1 mmHg, however, female students showed 114.5/75.4 mmHg lower than male (p<0.001). 9). In blood, for hemoglobin female students showed significantly lower level than that of male(p<0.001). T-Cholesterol and HDL-Cholesterol female students showed higher level than males (p<0.001), And for triglyceride male students showed higher level than females(p<0.001). Ca and Fe female students showed lower level (p<0.001).

• Journal of agricultural medicine and community health
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• v.33 no.2
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• pp.204-220
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• 2008

Objectives: This study was performed to determine the levels of depression symptoms among the community elderlies and to reveal its related factors, specifically aimed at revealing psycho-social factors such as social support net-work, sense of self-esteem, state anxiety, interpersonal behavior trait and locus of control. Methods: The interviews were performed during the period from April 1st to June 30th, 2007 to the 790 elderlies in urban and rural areas. Results: The mean scores of depression (CES-D) among all subjects were 16.65±10.97(urban: 18.81±10.31, rural: 14.49±11.20) and they were higher in urban than rural. The level of depression symptoms was influenced by the variables of educational level, with or without spouse, bear for living expenses, sense of satisfaction in daily life. As for health related behaviors, they were influenced by the variables of with or without regular exercise. As for health status, they were influenced by the subjective health status, with or without disability of body, visual acuity, hearing ability, ability of food mastication, urinary incontinence, with or without help in activity of daily living (ADL) and instrumental activity of daily living (IADL). As for social support net-work and social activities, they were influenced by number of offspring, frequency of offspring contents, frequency of going out and activity of hobbies. As for psycho-social factors, they were influenced by sense of self-esteem, state anxiety and social support, especially, the level of depression symptoms was higher influenced by the variables of psycho-social factors than other variables. Conclusions: Lower of the level of depression symptoms among the community elderlies would require development and application of programs to keep under management psycho-social factors as well as looking for ways to promoting the present health status.

• Korean Journal of Community Nutrition
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• v.17 no.5
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• pp.603-622
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• 2012

The population aged 95 years and older in Seoul approximately increased to five-fold over the past 10 years, while nationwide rates increased to three-fold. In order to examine the dietary habit and nutritional status of oldest-old population living in Seoul, we recruited 87 subjects (25 males and 62 females) aged 95 years and older. The prevalence of underweight (BMI < 18.5 $kg/m^2$) and obesity (BMI ${\geq}25kg/m^2$) were 18.2% and 18.2% in males, and 20.8% and 9.4% in females, respectively. In self-assessment of health, only 25.3% answered to be unhealthy. More males exercised regularly and reported a wide range of activities than females. The average of %Kcal from carbohydrate, protein and fat (C : P : F) was 64.9 : 13.8 : 21.2 in males and 68.1 : 14.2 : 17.7 in females. The average daily energy intake was 1,307 kcal in males and 1,304 kcal in females. More than 75% of subjects were taking under estimated average requirements (EAR) for vitamin $B_1$, $B_2$ & C and Ca. The average of mean adequacy ratio (MAR) was 0.66 in males and 0.70 in females, and 28.8% of males and 12.9% of females were in MAR < 0.50. Based on MAR, 32.0% of males and 14.5% females were classified as normal and 16.0% of males and 25.8% of females were classified as malnourished. Our subjects were taking more animal food, especially milk and its products, compared to those living, in rural areas. However, a significant proportion did not meet the EAR for vitamin $B_1$, $B_2$ & C and Ca.

• Journal of Community Nutrition
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• v.5 no.2
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• pp.105-111
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• 2003

Balanced intake of appropriate nutrients is the key to sustaining and promoting health as well as preventing and treating diseases. It is not always easy, however, to take balanced nutrition and various related factors must be taken into consideration. This report provides a summary of studies that have examined the nutrient intakes of Japanese middle-aged men and the relationship of this intake to drinking, job-related separation from families, and health practices. The alcohol consumption of Japanese middle-aged men has more than tripled in the last forty years. When nutrient intake was examined in relation to alcohol consumption, it was discovered that the maximum acceptable alcohol consumption was approximately 23 grams (about two drinks) of pure alcohol, provided the level of nutrient intake for drinkers was equal to that of non-drinkers. The alcohol energy ratio was approximately 5％. It was also discovered that middle-aged men's eating habits deteriorate when they relocate to new posts without their families and live by themselves. Compared to those living with their families, a higher proportion of those living alone have unfavorable eating habits including skipping breakfast or lunch, having a late lunch, and eating and drinking after dinner until bedtime. When Breslow's seven health practices, nutrient intake, and consumption weight by food group were examined, it was discovered that the group that had many beneficial eating and living habits consumed plenty of legume, pulses, fruit, green yellow vegetables and milk products. Their intake of vitamins and minerals was high and the results of a physical examination proved to be excellent. According to nutrition surveys conducted in Japan, China, the United Kingdom and the United States using a 24-hour recall method with common protocols and strict controls to ensure high levels of accuracy and cross-study validity, the Japanese had the highest cholesterol intake and the lowest dietary fiber intake among the four countries. Also, the alcohol energy ratio of the Japanese exceeded 8％, the highest among the four countries, while their intake of magnesium and iron was the lowest These results indicate that it is necessary to enhance nutritional education for middle-aged men and to reinforce the social environments in which they live and work in order to promote proper diet and nutrition in Japan. (J Community Nutrition 5(2) : 105-111, 2003)

• Korean Journal of Rural Living Science
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• v.2
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• pp.1-23
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• 1991

농촌주민들의 영양 섭취실태에 대한 동향을 요약해 보면 1. 총 섭취식품중 약 90% 정도를 식물성식품으로써 섭취하고 있으며, 곡류가 차지하는 비율은 점차 감소되는 반면 콩류, 채소류 및 과일류의 섭취 비율은 현저히 증가하고 있다. 2. 총 열량섭취는 감소하는 추세이고 총열량에 대한 탄수화물 :단백질 :지방의 비율이 74 : 12 : 12의 수준이다. 총 단백질 섭취에 대한 동물성 단백질 섭취율은 36%로써 질적으로 바람직하게 변화되고 있다. 칼슘의 섭취수준이 권장량의 90%정도로서 조금 부족한 듯 하지만 철분, 비타민 등 대체로 영양섭취상태는 많이 개선되었음을 알 수 있다. 3. 농촌 영아들의 경우는 모든 영양소가 권장량에 현저히 미달되는 상태이다. 이유식품 개발 및 이유 방법에 대한 상담 지도가 적극 필요한 실정이다. 4. 새마을 유아원의 설치 및 유아급식의 혜택으로 농촌 유아들의 영양 섭취 상태는 두드러지게 개선되었으나 철분섭취 상태는 권장량에 미달되는 실정이다. 취학전 아동의 영양향상을 위해서는 유아 교육시설 확충 및 간식의 중요성에 대한 농촌 주부 대상의 영양 교육 확대 실시가 요청된다. 5. 국민학교 아동들에게서는 빈혈의 발생 빈도가 높게 나타났다. 성장 발육기에 있는 이들의 심신발달에 필요한 영양공급 및 올바른 식습관 형성을 위해서는 학교에서 일정한 지도 목표를 설정하여 계획적으로 실시하는 학교 급식의 필요성이 강조된다 하겠다. 6. 청소년기 및 임신 수유부의 경우도 철분의 섭취상태가 부족하기는 하지만, 철 결핍성 빈혈증상이 나타날 정도는 아니고 영양섭취상태도 대체로 양호하다. 7. 노인들의 식품 영양 섭취실태는 권장량에 크게 미달되는 실정이기는 하지만 혈액검사의 수치들이 대체로 정상인 범위에 있고 건강상태도 양호한 편이다. 식품 개발 및 조리법에 관해서 요약해 보면 1. 식량수급의 안정을 기하고자 다수확 품종인 통일계통 쌀의 취반 및 가공적성에 대한 검토가 진행되었으며, 잡곡류 및 감자류의 효과적인 이용을 위해 복합분의 사용에 의한 식품가공 적성에 대한 연구들이 실시되었다. 2. 콩류에 대한 연구로는, 기름을 추출하고 남은 탈지대두박의 단백질 식품으로의 이용을 위해 추출법의 검토 및 탈지대두박을 이용하는 두부제조법 등 다각적으로 식품에의 적용에 관해 연구가 진행 되었다. 3. 발효식품 중 김치에 대해서는 농촌지역에서 제조되는 김치의 종류와 섭취실태에 관한 연구 및 김치의 저장성을 증진시키기 위한 일련의 검토가 있다. 간장, 고추장, 된장에 대해서는 Koji를 이용한 개량식 제조법 및 팽화시킨 곡분을 사용하는 간편 제조법, 비용 절감을 위한 대체원료 이용에 대한 연구 등이 진행되었다. 4. 이밖에도, 병조림 제조방법, 어린이를 위한 고영양 보충식 개발, 생선의 폐기 부분을 이용하여 칼슘 급원 식품으로서의 유용성에 관한 조리과학적 검토 등이 실시되었다. 식습관 및 식생활 관리 지도에 관한 연구에 의하면, 농촌여성의 농업역할이 가중되면서 자녀들의 식생활을 방임하기 쉬운 실정이므로 유아 교육시설 및 학교 급식 등을 통해서 바람직한 식습관 형성을 위한 프로그램이 개발되어져야 하며, 한편, 식생활 담당자인 농촌주부에 대한 규칙적이고 정확한 영양교육이 이루어져야 하리라 본다.

• Ocean and Polar Research
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• v.31 no.1
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• pp.121-131
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• 2009

In an effort to better understand the physiological and ecological differences between warm and cold water copepod species in Korean waters using lipid contents and compositions, two species of copepods (Pleuromamma sp. as a warm water species and Neocalanus plumchrus as a cold water species) were collected from the Northwest Pacific and East Sea/Sea of Japan, respectively. The cold water species showed two fold higher lipid contents than the warm water species (11% vs. 5% of dry weight). Wax esters, known as one of the major storage lipid classes, were found to be the dominant lipid class (accounting for 64% of total lipids) in the cold water species, whereas, in the warm water species, phospholipids, which are known as membrane components, were the dominant lipid class (accounting for 43% of total lipids),with a trace amount of the storage lipids as a form of triacylglycerols (${\leq}1%$ of total lipids). With regard to the fatty acid compositions, saturated fatty acids (SAFA), especially 16:0 (about 30% of total fatty acids), were most abundant in the warm water species, whereas the polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA : 20:5(n-3)) (${\geq}16%$ of total fatty acids), were most abundant in the cold water species. Among the neutral fraction of lipids, phytol, originating from the side chain of chlorophyll and indicative of active feeding on phytoplankton, was detected only in the warm water species. Significant quantities of fatty alcohols were detected in cold water species, particularly long-chain monounsaturated fatty alcohols (i.e. 20:1(n-9) and 22:1(n-11)), which are well known to abound in cold water herbivorous copepods. However, only trace amounts of short-chain fatty alcohols were detected in the warm water species. Twelve different kinds of sterols were detected in these copepod species, with cholest-5-en-$3{\beta}$-ol (cholesterol) and cholesta-5, 24-dien-$3{\beta}$-ol (desmosterol) dominating in cold and warm water species, respectively. In addition, for the warm water species (Pleuromamma sp.), we assessed the latitudinal gradients of lipid contents and compositions using samples from three different latitudinal regions (Philippine EEZ, Japan EEZ, and the East China Sea). Although no latitudinal gradients of lipid contents were detected, the lipid compositions, particularly dietary fatty acid markers, varied significantly with the latitude. The findings of this study confirm that the distribution of lipid contents and compositions in copepods may not only indicate their nutritional condition and diet history, but may also provide insights into their living strategies under different environmental conditions (i.e., water temperature, food availability).

• The Korean Journal of Rehabilitation Nursing
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• v.5 no.2
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• pp.180-192
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• 2002

The purpose of this study was to survey basic data for management of elderly day care centers. In this study, occupation, leisure life, and food, clothing and habituation, as well as social relationship, health state and behavior, and general characteristics of subjects were examined to identify the factors affecting their cognition, need and preference of the day care centers. The subjects of this study were 392 elderly, aged 60 years or over, living in Gangnung and neighboring districts. Data were collected by educated interviewers from November 4 through November 22, 2002. The subjects were interviewed face to face, one for one after the interviewee's agreements on the survey. The collected data were analysed with logistic regression analysis by SAS (statistical analysis system). Logistic regression analysis was done to identify affecting factors for cognition, need and preference of the elderly day care centers. The major findings are as follows: 1. The factors affecting cognition for the day care centers were analysed. The subjects with an occupation and a lower satisfaction level of living environment and friendship, who were economically secure enough to manage a sudden accident, showed a higher level of cognition of the day care centers. The subjects with a higher level of mental health state and a lower level of IADL also showed a higher level of cognition. On the general characteristics younger female subjects showed a higher level of cognition. 2. The subjects with a lower level of perceived economic condition who did not own their housing and were not economically safe enough to manage a sudden accident, had showed a higher level of need for the day care centers. It showed that the subjects with a high level of mental health state, a bad eye sight and dental condition, a good perceived health condition, and a lower level of IADL, needed the centers. 3. The subjects who had an occupation, however, not capable of making their own daily expenses, and a low occupation satisfaction level, and who did not own their housing, and were economically poor not enough to manage a sudden accident, showed a higher preference for the day care centers. The subjects with higher levels of friendship satisfaction and perceived health condition, not living with their spouse, and a higher education level, showed a higher preference for the centers. In conclusion, the common factors affecting their cognition, need and preference of the day care centers were occupation, economic security enough to manage accidents, and friendship satisfaction level. Especially, the subjects who had an occupation, however, not economically secure enough to manage accidents, and who did not live in their own housing with a good perceived health condition, showed high levels of need and preference for the day care centers. These results can be used as basic data to develop the efficient elderly day care centers, thus contribute to the elderly welfare in a local community.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.415-422
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• 2021

Cotton is an important fiber crop, and its seeds are used as feed for dairy cattle. Crop biotechnology has been used to improve agronomic traits and quality in the agricultural industry. The frequent unintentional release of LM cotton into the environment in South Korea is attributed to the increased application of living modified (LM) cotton in food, feed, and processing industries. To identify and monitor the LM cotton, a method for detecting the approved LM cotton in South Korea is required. In this study, we developed a method for the simultaneous detection of four LM cotton varieties, MON757, MON88702, COT67B, and GHB811. The genetic information of each LM event was obtained from the European Commission-Joint Research Centre and Animal and Plant Quarantine Agency. We designed event-specific primers to develop a multiplex PCR method for LM cotton and confirmed the specific amplification. Using specificity assay, random reference material(RM) mixture analysis and limit of detection(LOD), we verified the accuracy and specificity of the multiplex PCR method. Our results demonstrate that the method enabled the detection of each event and validation of the specificity using other LM RMs. The efficiency of multiplex PCR was further verified using a random RM mixture. Based on the LOD, the method identified 25 ng of template DNA in a single reaction. In summary, we developed a multiplex PCR method for simultaneous detection of four LM cotton varieties, for possible application in LM volunteer analysis.