• 한국식품과학회지
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• 제35권3호
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• pp.429-435
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• 2003

가정배달급식의 저장기간 중 식중독 발생에 대비하여 식중독균의 증식 양상을 사전에 예측하기 위한 기본자료를 얻고자 표고고기전, 갈치조림, 더덕구이에 주요 식중독 원인균을 접종하여 wrap 포장, 상압 및 진공포장 처리한 후, -18, 4, $25^{\circ}C$에서 5일간 포장방법 및 저장조건에 따른 변화를 살펴보았다. 가정배달급식을 위한 음식에 주요 식중독균을 접종한 결과, 식중독균의 성장 및 증식은 적용된 음식을 진공포장 처리하여 냉장 및 냉동저장시에 억제되는 것으로 입증되었고, 접종된 주요 식중독균의 포장방법 및 저장조건에 따른 성장 및 증식은 저장온도에 유의적인 영향을 받는 것으로 나타났으며, 특히, L. monocytogenes는 저장기간에 대해서도 유의적인 영향을 받는 것으로 나타났다. 따라서 표고고기전과 갈치조림의 냉장저장 시에는 L. monocytogenes가 저장 5일에 증가하므로 음식품질을 유지하고 식중독균 발생을 방지하기 위해서는 3일까지 저장하는 것이 바람직한 것으로 판단되었다.

• Preventive Nutrition and Food Science
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• 제17권2호
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• pp.172-176
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• 2012

Doenjang is a traditional Korean fermented soybean product that provides a major source of protein. In this study, a total of 18 different home-made doenjang samples were examined for the presence of foodborne pathogens and the total aflatoxin levels. Using an enzyme-linked immunosorbent assay to assess microbial quality and potential public health risk, we showed that total coliform levels in the doenjang samples ranged from 0 to $4.43{\pm}2.32{\times}10^6\;CFU/g$, and the maximum limit of Bacillus cereus was $4.67{\pm}2.0{\times}10^5\;CFU/g$. However, other foodborne pathogens, such as Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella spp., were not detected among the tested samples. One of the samples (S3) showed a maximum limit of $42.2{\pm}9.1\;{\mu}g/kg$ for aflatoxin levels, which was above the safety limit allowed by the Codex Alimentarius Commission (CAC) regulatory agency. Further research is necessary to determine whether and how doenjang safety can be improved via elimination/reduction of microbial contamination during fermentation and storage or using microbial starter cultures for its fermentation.

• 대한영양사협회학술지
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• 제19권3호
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• pp.236-252
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• 2013

The purpose of this study was to assess the microbiological quality of home-delivered meals during production and delivery for children from low-income families. Production flows from a facility in Seoul that provides home-delivered meals were analyzed and the time-temperature of the food was measured. Microbiological assessment was performed for the production environment, personal hygiene, and food samples at each production and delivery step based on the process approach. It took 2 hours or longer from completion of production to meal delivery. An aerobic colony count (ACC) and coliform were not detected at knives, cutting boards, and dish towels. However, ACC (at pre-preparation, preparation, and packing areas) and coliform (at the preparation area) were detected on the hands and gloves of employees. Air-borne bacterial counts varied according to day and preparation area (ND~6 CFU/plate/15 min). Food temperatures, on the completion of production and meal delivery, fell into temperature danger zones. ACC and coliform counts of raw ingredients did not decrease after pre-preparation (washing and sanitizing) for menus involving food preparation with no cook step. ACC decreased after cooking step for menus of food preparation with cook step, but the ACC of the stir-fried and seasoned dried filefish fillet on the completion of cooking was too numerous to count due to improper heating. The ACC of seasoned young Chinese cabbages (a menu with complex food preparation) increased during delivery (from 2.5 log CFU/ml to 5.0 log CFU/ml). This qualitative assessment of foodborne pathogens revealed that B. cereus was detected in vegetable and meat product menus. These results suggest time-temperature control is necessary during production and delivery and management guidelines during production of home-delivered meals are provided for safe production.

• 한국축산식품학회지
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• 제33권2호
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• pp.149-154
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• 2013

Food hygiene practices must be maintained from farm to table in order to prevent contamination by microorganisms. This study was conducted to investigate consumer hygiene practices related to the refrigerator storage of meat, including a microbial analysis, monitoring of refrigerator temperatures and consumer surveys of female homeowners in the capital area of Korea. Home refrigerator temperatures were maintained above $5^{\circ}C$ in 26 (19.7%) of the 132 houses investigated. The percentage of the refrigerators with a total microbial count over $10^2\;CFU/100\;cm^2$ was 14.4%. No E. coli, Salmonella spp. or Listeria monocytogenes microbes were detected. However, Staphylococcus aureus was detected in 14 houses (10.6%). The only statistically significant difference in hygiene practices between the non-contamination group and contamination group was in the last time of refrigerator cleaning (p<0.01), as determined by the consumer survey. To improve food hygiene when using a refrigerator, raw materials must be packaged, meat should be stored only on a designated shelf, and cooked foods must be contained to prevent cross-contamination. The refrigerator should be cleaned regularly, at least once a month, and refrigerator thermometers should be monitored below $5^{\circ}C$ in order to keep food safe.

• 한국식품위생안전성학회지
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• 제31권3호
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• pp.201-209
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• 2016

실내 공기질 관리의 중요성이 대두되면서 쾌적한 실내 환경에 도움을 주는 공기청정 기능과 습도 조절 기능을 동시에 갖춘 제습기와 공기청정기가 각광받고 있다. 하지만 오랜 기간 동안 공기정화제품을 사용하게 될 시에는 필터가 오염되어 본연의 기능을 상실하게 되는 것으로 알려져 있지만 이에 대한 구체적인 연구나 보고는 드문 편이다. 이에 본 연구에서는 가정과 사무실 등에서 사용한 공기정화제품을 수거하여 주요 부위별 미생물 오염도 및 주요 오염 미생물들을 분석하였다. 그 결과, 4 종류의 공기정화제품에서 오염도가 높은 부위는 필터부위, 물이 직접 닿는 부위 및 공기가 외부로부터 직접적으로 들어오는 입구부위 등에서 미생물학적 오염도가 가장 높았다. 하지만 공기정화제품은 사용하는 환경과 조건에 따라서 미생물학적 오염도 및 오염 미생물의 성상은 각각 다르게 나타났다. 하지만 이들 공기정화제품들에는 공통적으로 Staphylococcus sp., Micrococcus sp. 그리고 Bacillus sp.의 세균과 Cladosporium sp. 및 Penicillium sp.의 진균이 공통적으로 오염되어 있는 우점종인 것으로 분석되었다.

• 한국축산식품학회지
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• 제23권4호
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• pp.342-349
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• 2003

Escherichia coli O157:H7, Staphylococcus aureus와 Salmonella enteritidis는 식품에 의해 전염되는 식품질환성 병원균으로서 세계적으로 알려진 식중독 미생물이다. 오미자 물 추출액 첨가 drink yoghurt에 오염된 식중독 유발균인 Esc. coli O157：H7, Sta. aureus와 Sal. enteritidis의 생존에 미치는 영향을 연구하였다. 오미자 물 추출액이 식중독 유발균에 대한생육 저해 활성을 검토하였고, 시험 균주의 최종 균체 농도가 $10^{5}$ CFU/mL 수준이 되도록 오미자 물 추출액 첨가 drink yoghurt에 Esc. coli O157：H7, Sta. aureus와 Sal. enteritidis를 각각 접종하여 37$^{\circ}C$에 배양하면서 시간별로 시험 균주들의 생존 균수를 측정하였다. 시험 결과 시험에 사용된 오미자 물 추출액의 첨가 농도가 증가할수록 모든 시험 균주에서 강한 증식 억제력이 나타났다. 대조구에 비해 오미자 물 추출액 0.4 ,0.6, 0.8과 1.0% 첨가구에서 Esc. coli O157：H7은 각각 0.13, 0.89, 1.99와 2.55의 log cycle 감소 현상을 보였고, Sta. aureus는 각각 0.45, 3.74, 4.13과 5.24의 log cycle이 감소되었으며, Sal. enteritidis는 각각 0.22, 3.44, 4.02와 4.07의 log cycle 감소가 나타나 모든 시험 균주에서 뚜렷한 성장 억제 효과가 관찰되었다. 오미자 물 추출액의 식중독 유발균에 대한 성장 저해 효과는 Sta. aureus, Sal. enteritidis, 그리고 Esc. coli O157：H7의 순으로 강하게 나타났다. 오미자 물 추출액을 0.4, 0.6, 0.8과 1.0% 첨가한 drink yoghurt 내에서 3.55${\times}$$10^{5}$ CFU/mL 수준으로 접종한 Esc. coli O157:H7은 24시간 배양시 1.00${\times}$$10^1$∼3.00${\times}$$10^1$ CFU/mL로 감소되었으며, 48시간 배양하였을 때에는 생육이 완전히 억제되었다. 그리고 1.24${\times}$$10^{5}$ CFU/mL 수준의 Sta. aureus를 접종한 경우 24시간 배양 후 아주 미약한 생존 균수의 감소를 보였으나 48시간 배양시 4.00${\times}$$10^2$∼8.50${\times}$$10^2$ CFU/mL 수준으로 감소하였고, Sal. enteritidis를 1.81${\times}$$10^{5}$ CFU/mL 수준으로 접종한 경우 24시간 배양 후부터 전연 증식을 나타내지 않았다. 이와 같이 오미자 물 추출액 첨가에 의해 drink yoghurt 내에서 식중독 유발균들의 증균 억제 효과를 확인할 수 있었다.할 수 있었다.

• 한국식품위생안전성학회지
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• 제12권3호
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• pp.240-253
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• 1997

The main problems contributing to food poisoning outbreaks in institutional settings and a home were reviewed and analyzed through the epidemiological investigations of food poisoning. The major documented factors included improper holding temperatures, inadequate cooking, poor personal hygiene, cross-contamination and contaminated equipment, food from unsafe sources, failure to follow food hygiene policies, and lack of education, training, monitoring and superivision. Usually more than one factor contributed to the development of an outbreak. (1) Use of improper holding temperatures was the single most important factor contributing to food poisoning. They included improper cooling, allowing a laps of time (12 hours or more) between preparing food and eating it, improper hot holding, and inadequate or improper thawing. Food thermometers were not used in most of the instances. (2) In inadequate cooking, the core temperature of food during and after cooking had not been measured, and routine monitoring was limited to recording the temperature of plated meals. Compared with conventional methods of cooking, microwave ovens did not protect against food poisoning as effectively. Centralized food preparation potentially increased the risk of food poisoning outbreaks. (3) Poor personal hygiene both at the individual level (improper handwashing and lack of proper hygienic practices) and at the institutional level (poor general sanitization) increased the risk of transmission. Person to person transmission of enteric pathogens through direct contact and via fomites has been noted in several instances. (4) Obtaining food from unsafe sources was a risk factor in outbreaks of food poisoning. Food risks were high when food was grown or harvested from contaminated areas. Possibilities included contamination in the field, in transport, at the retail site, or at the time it was prepared for serving. (5) Cross-contamination and inadequate cleaning/handling of equipment became potential vehicles of food poisoning. Failure to separate cooked food from raw food was also a risk factor. (6) Failure to follow food hygiene policies also provided opportunities for outbreaks of food poisoning. It included improper hygienic practices during food preparation, neglect of personnel policies (involvement of symptomatic workers in food preparation), poor results on routine inspections, and disregarding the results and recommendations of an inspection. (7) Lack of formal and in-service education, training, monitoring, and supervision of food handlers or supervisors were critical and perhaps neglected elements in occurrences of food poisoning.

• 한국식품위생안전성학회지
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• 제11권4호
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• pp.277-285
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• 1996

Retrospective study on the comparison of outbreaks of food poisoning for food hygiene in Korea and Japan. The average value of morbidity rate by year in Korea during the period of 1971 to 1992 was 2.9 per 100,000 population, and that of Japan was 29.1. The mean value of mortality rates in case of food poisoning by year in Korea was 2.33%, and that of Japan was 0.07%. When compared the rates of morbidity and mortality between Korea and Japan during the same period, the morbidity rates of Japan were much higher than those of Korea (p<0.01). However, mortality rate of patients in Korea were much higher then those of Japan(p<0.01). Resulting from comparative observation of food poisoning by preparing facilities between Korea and Japan. The highest list the places where the outbreaks occurred was home-made foods accounted for 48.8% of the total cases in Korea and that of Japan was restaurants accounted for 33.0%. Causative foods in Korea, the most common incrimination vehicles were seafood, meat and animal products and grain and vegetables, including mushroom. However, in the case of the common incrimination vehicles Japan were unknown and other foods, seafood, vegetables and meat and animal products etc.. Food poisoning of pathogenic substance in Korea were 60.9% of bacterial food poisoning of the total cases showing that Vibrio species, Salmonella spp., Staphylococcus spp., pathogenic E. coli, Clostridium spp. and other spp. were 33.3%, 26.2%, 16.3%, 5.3%, 0.4% and 18.5%, respectively. On the other hand, in Japan, major causes were Vibrio spp. (45.7%), Staphylococcus spp. (23.7%), Salmonella spp. (16.8%), pathogenic E. coli (3.8%), Clostridium spp. (0.2%) and other spp. (9.6%).

• 한국식품위생안전성학회지
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• 제26권3호
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• pp.248-253
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• 2011

우리나라는 2인 이상의 집단 식중독에 대해서 식중독 발생건수와 환자수에 대해서 통계집계를 실시하고 있지만 산발적으로 발생하는 1인 식중독에 대해서는 집계 되고 있지 않다. 실제 발생하는 식중독을 식중독 통계에 근접하게 집계하는 방안의 하나로써 l인 식중독의 비중 및 패턴을 파악하고자 한국과 식이 패턴이 유사한 일본의 통계 (2002-2003)를 이용하여 1인 식중독과 2인 이상 집단식중독을 비교분석 하였다. 아울러 한국의 인구수 대비 식중독 발생 및 보고 비율을 일본과 비교하였다. 일본의 1인 식중독이 전체 식중독에서 차지하는 비율은 발생 건수에서 43.5%이며 발생장소는 장소불명 (90-92.3%)과 가정집 (6.2-8.5%)이 대부분이었다. 일본의 l인 식중독에서 C. jejuni (51.9%), Salmonella spp. (35.3%), V parahaemolyticus (9.5%)가 원인균의 대부분을 차지하였다. 한편 2인 이상 집단 식중독의 원인균은 norovirus (3l.3%), Salmonella spp. (20.8%), C. jejuni (15.5%)로 나타났다. 특히, 집단 식중독이 자주 발생한 장소는 음식점 (46.6-50.1%)과 여관(9.2-9.8%)이었고 1인 식중독보다 주로 집단 식중독에서만 발생한 식중독 원인균은 norovirus, S. aureus, Cl. perfringens, unknown였다. 한국과 일본의 식중독 발생 및 보고 비율은 2002-2009년 통계를 비교한 결과 2인 이상의 집단 식중독을 기준으로 1:1.5 수준이었다.

• 한국지역사회생활과학회지
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• 제16권3호
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• pp.87-96
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• 2005

This survey was conducted to assess the perception of hazards and washing behavior of vegetables of 500 housewives in Korea. The subjects were selected by the stratified random sampling method. The survey was performed using a structured questionnaire through telephone interviews by skilled interviewers. Most people have bought vegetables and fruits in the supermarket or (traditional) markets rather than stores, department stores, or direct transactions. Eighteen percent of the subjects felt vegetables were secure and were not concerned about safety. But $42.8{\%}$ were concerned about vegetable safety. The perceptions of vegetable-related hazards differed significantly by the respondent's socioeconomic characteristics. Higher concern about vegetable safety was reported by subjects with higher income, children, and who usually buy vegetables in supermarkets or department stores. Most subjects ($88.6{\%}$) perceived that residues of chemical substances such as pesticides were the most significant potential vegetable risk factor, followed by heavy metal, and pathogens. Housewives mainly rinsed vegetables in flowing-water ($85.2{\%}$ of subjects), 3${\~}$4 times ($63.8{\%}$ of subjects), and without detergent ($90.6{\%}$). Subjects believed that hazards decreased by blanching or boiling vegetables rather than washing. Subjects realized more or less correctly the removal rate of pesticide and pathogen through the washing and cooking processes. However, the removal rate of heavy metals was less than subjects thought it would be. Therefore, the scientifically assessed results on safety in the washing and cooking process should be opened to the public to provide the right-to-know and assure confidence in consumers.