• 대한본초학회지
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• 제38권2호
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• pp.17-26
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• 2023

Objectives : A persistent inflammatory response can cause diseases such as fibrosis, cancer, and allergies. This study aimed to investigate the anti-inflammatory activity of Curcumae longae Rhizoma and Cinnamomi Ramulus Mixture (CCM) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Methods : The total polyphenol and flavonoid contents of CCM were confirmed through an in vitro experiment. Also, radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Hydroxyl were confirmed. Moreover, ferric reducing antioxidant power (FRAP) activity were confirmed. After, CCM (50, 100, and 200 ㎍/mL) were applied to 0.1 ㎍/mL LPS-stimulated RAW264.7 cells. The levels of nitric oxide (NO) and pro-inflammatory cytokines in the supernatant fraction were determined. Also, the expressions of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways were detected using Western blot. Results : As a result of in vitro experiments, there was an excellent antioxidant activity in CCM-treated cells. In addition, in RAW264.7 cells stimulated with LPS, the increased NO level was inhibited in a concentration-dependent manner by the treatment of CCM. In addition, inflammatory cytokines production were significantly inhibited in a concentration-dependent manner in CCM-treated group. CCM treatment significantly decreased the protein expressions of MAPKs. Moreover, the expressions of NF-κBp65 and cyclooxygenase-2 (COX-2) were significantly decreased when 200 mg/kg of CCM was applied, and phospho-inhibitor of nuclear factor kappa B-α (p-IκBα) and inducible nitric oxide synthase (iNOS) were significantly decreased at all concentrations treated with CCM. Conclusion : Our findings show that CCM exhibited excellent antioxidant activity and exhibited superior anti-inflammatory effect through the MAPKs and NF-κB pathways in LPS-stimulated RAW 264.7 macrophages.

• 대한임상검사과학회지
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• 제50권1호
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• pp.37-43
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• 2018

본 연구는 전라도 장성지역의 편백나무 잎 추출물을 사용하여 in vitro에서 항염증 및 항알레르기 효과를 보고자 연구를 진행하였다. 편백나무 잎 추출물은 $50^{\circ}C$에서 감압 건조하여 실험에 사용하였으며, 총 폴리페놀 함량을 측정한 결과 $25.89{\pm}0.31mg\;GAE/g$로 나타났다. 설정된 GC-MS 분석법으로 편백나무잎 추출물의 6종 성분에 대한 함량 분석을 실시한 결과, ${\alpha}-Terpinene$ 3.03 mg/g, ${\alpha}-Terpineol$ 9.48 mg/g, limonene 5.96 mg/g, borneol 59.78 mg/g, myrcene 4.85 mg/g, sabinene 11.31 mg/g로 borneol이 가장 많은 것으로 나타났다. 편백나무 잎 추출물의 항산화 활성을 측정한 결과, $H_2O_2$와 $ABTS^+$ 라디칼에 대한 추출물의 $RC_{50}$이 각각 $5.47{\pm}0.13mg/mL$와 $4.00{\pm}0.01mg/mL$로 나타났다. 또한 마우스 유래의 대식세포주인 RAW 264.7 세포에서 LPS 100 ng/mL을 처리를 통한 염증유도 군에서 주요 인자인 NO 생성이 $28{\pm}0.38{\mu}M$까지 증가하였으나 편백나무 잎 추출물 $150{\mu}g/mL$ 처리 농도에서 $IC_{50}$ 으로 감소할 것으로 추정되므로, 편백나무 잎 추출물이 항염증 작용이 있음을 시사한다. 알레르기 주요 인자인 ${\beta}-hexosaminidase$의 경우 처리한 편백나무 잎 추출물의 농도 의존적으로 감소되어 항알레르기 효능이 있음을 알 수 있었으며, 인간 유래 섬유아세포인 CCD-986sk 세포에 대해 편백나무 잎 추출물의 농도 $100{\sim}800{\mu}g/mL$ 범위에서 세포독성을 전혀 보이지 않았다. 따라서 편백나무 잎 추출물이 향후 기능성 화장품, 연고 등의 산업화에 광범위하게 이용될 수 있을 것으로 사료된다.