• Journal of Applied Biological Chemistry
• /
• v.56 no.2
• /
• pp.119-129
• /
• 2013

Thirteen different Streptomyces isolates were evaluated for their ability to produce keratinase using chicken feather as a sole carbon and nitrogen sources under solid state fermentation (SSF). Streptomyces sp. NRC 13S produced the highest keratinase activity [1,792 U/g fermented substrate (fs)]. The phenotypic characterization and analysis of 16S rDNA sequencing of the isolate were studied. Optimization of SSF medium for keratinase production by the local isolate, Streptomyces sp. NRC13S, was carried out using the one-variable-at-a-time and the statistical approaches. In the first optimization step, the effect of incubation period, initial moisture content, initial pH value of the fermentation medium, and supplementation of some agro-industrial by-products on keratinase production were evaluated. The strain produced about 2,310 U/gfs when it grew on chicken feather with moisture content of 75% (w/w), feather: fodder yeast ratio of 70:30 (w/w), and initial pH 7 using phosphate buffer after 8 days. Based on these results, the Box-Behnken design and response surface methodology were applied to find out the optimal conditions for the enzyme production. The corresponding maximal production of keratinase was about 2,569.38 U/gfs.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2003.10b
• /
• pp.40-42
• /
• 2003

In order to examine the possibility that oil seed rape could be used as a forage fodder crop and to select the most suitable variety of forage rape at the southern area of Korea, two varieties of oil seed rape currently grown for oil production and six introduced varieties of forage rape with relatively high yield and high nutritional value were grown at the same condition and yield components were observed. Forage rape was superior to oil seed rape in terms of yield components, plant fresh weight and plant dry mater weight. Velox was superior to any other variety of forage rape in these characters. When plant dry matter weight of the rape was subdivided into four components such as a main stem, branch stems, main stem leaves and branch leaves, contribution of these components to plant dry matter weight was in the order of branch stems, branch leaves, the main stem and main stem leaves. Dry matter percentage of the rape ranged from 9.32 to 11.08 percent, which was somewhat low value. There was no significant difference between two groups of the rape in terms of dry matter percentage. Velox showed somewhat higher value in dry matter percentage.

• Korean Journal of Organic Agriculture
• /
• v.8 no.3
• /
• pp.17-34
• /
• 2000

This paper is aim to report the core aspects of Codex guideline for organically grown food which is finalized by FAO/WHO Codex Alimentarius Commission on May 2000 in 28th session of the Codex committee on food labelling. The chapter of animal production had discussed for a long time before it was finalized in Ottawa/canada as well as use of GMO(Genetically modified organism), manure from factory farming, animal welfare, and fodder inputs consisting of at least 85% for ruminants and 80% for non-ruminants. As the guideline for Codex set out the several things which is very difficult for Asian country, Organic farmer in Korea should pay an attention to maintain/increase the fertility and biological activity by cultivation of legumes, green manures or deep-rooting plants in an appropriate multi-annual rotation programme, and incorporation in the soil of organic material from holding producing in accordance with the guidelines. Pest, diseases and weeds should be controlled by choice of appropriate varieties, appropriate rotation, mechanical cultivation, diversified ecosystems, flame weeding, animal weeding and steam sterilization. The use of plant growth hormone, GMO and manure from industrial management system are not allowed, and closed recycling system, rotation, resistant seeds again pest and disease should be practiced in organic farming. But these are not unfortunately practiced in the country. In the conclusion it was strongly suggested to enact the Basic Standard for Korean organic agriculture which contains the core principles of Codex guidelines, and to try the importation of the most advanced theory, skills and technology from leading country in organic farming.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.4
• /
• pp.487-489
• /
• 2000

An experiment was conducted for 56 days using nine castrated male Black Bengal goats (initial live weight 9.0 kg) aged about 5 months to study the effects of feeding Sesbania leaves as a sole feed on growth performance and nutrient utilization. The animals were fed ad libitum on either road-side grass, Sesbania aculeata leaves or Sesbania rostrata leaves. Average dry matter (DM) intake was 179, 229 and 259 g/d for goats fed road-side grass, Sesbania aculeata and Sesbania rostrata, respectively. Corresponding mean values for average daily live weight change were -6.6, 9.5 and 38.1 g, respectively. These values were higher (p<0.05) for goats fed Sesbania leaves than those given road-side grass. The apparent digestibility of all the proximate components except CF and the concentrations of DCP, TDN, DOM and ME of Sesbania leaves were higher (p<0.05) than those of road-side grass. Sesbania rostrata was superior (p<0.05) to Sesbania aculeata for nutrient digestibilities (except for NFE) and also for DOM, DCP and ME concentrations. Therefore, it may be concluded that the production of Sesbania rostrata, a legume fodder, should be encouraged for feeding goats.

• Journal of agricultural medicine and community health
• /
• v.21 no.1
• /
• pp.13-20
• /
• 1996

Paederus dermatitis caused by the contact of staphylinid beetles(Paederus fuscipes) body fluid(pederin) is characterized by linear erythematous vesicopustular lesions. An outbreak of dermatitis occurred at apartments located at the river side after rainy season in summer 1994. Authors surveyed 189 cases(94 males and 95 females) from 103 households reported at the apartment office in Kimcheon on September 1994. The results are as follows; 1. The causative agent of skin diseases was identified as Paederus fuscipes. 2. The attack rate of paederus dermatitis was 52.6% by households, and the residents in the middle floors of the apartments were attacked more frequently than those in other floors. All ages were affected. 3. The skin lesions developed by paederus were distributed on face(38.2%), neck(18.5%), shoulder and trunk(11.0%), arm and leg(10.3%), and back(6.6%). In 21 cases(15.4%), skin lesions were developed in multiple sites. 4. The apartments attacked by paederus was located near the sand and damp soil along the bank of the river. The fodder cultivating farm was located near the upper reaches of river, and a rice field and grape garden in the backyard of the apartment.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.4
• /
• pp.474-478
• /
• 2001

Eighteen Murrah male buffalo calves were allotted into three groups of six each. The calves in group I (control) were fed with whole milk, skim milk, calf starter and green maize fodder. The calves in group II (high butyric acid) and group III (low butyric acid) were fed with the same diet as control along with 24 ml and 12 ml of butyric acid/calf/day for 120 days, respectively for 120 days. Dry matter intake was higher in group II and III as compared with group I. Digestibility of dry matter, organic matter, crude protein, crude fibre, ether extract, neutral detergent fibre, acid detergent fibre, cellulose and hemicellulose was the highest in group II followed by group III and the control group. Body weight gain and conversion efficiency of dry matter, digestible crude protein and total digestible nutrients were better in group II. Cost of feed for per unit of live weight gain was the lowest in group II. It was concluded that dietary addition of butyric acid (24 ml/day) was economical and had positive effect on the performance of Murrah buffalo calves.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.5
• /
• pp.643-649
• /
• 2002

Availability of feeds and fodder and amount of their intake by large ruminants in eight different agro-ecological zones (AEZ) of Bangladesh were recorded over a year. Roughages such as straw, naturally grown green grass, water hyacinth, tree leaves, legumes and sugarcane tops were the major feedstuffs fed to the large ruminants. Amount of intake of these ingredients differed (p<0.01) across AEZ studied, but did not differ across seasons or farmers' categories (p>0.05) except for green grasses (p<0.01). Byproduct concentrates offered to animals in the studied areas were rice bran, wheat bran and different oil cakes. The amount of intake of concentrates by the animals also differed (p<0.01) across AEZs, but did not differ across seasons or farmers' categories (p>0.05), except for rice bran and wheat bran which differed between season and farm category, and season respectively (p<0.01). The large standard errors of mean indicate that there are wide variations in intake of roughages and concentrates across AEZ's, seasons and farmers' categories and even across days. This further indicates that the feeding practices of large ruminants are largely heterogeneous. In addition to feeding roughage and concentrates, the animals were allowed to graze for six hours a day. Grazing hours also differed (p<0.01) across AEZ, but not by farmers' categories or seasons.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.6
• /
• pp.891-894
• /
• 1999

A field experiment was conducted with Calliandra calothyrsus of fifteen provenances to evaluate the variation in the condensed tannin content of fresh leaves. Plants were raised in a nursery for three months and planted in the field. Experimental design was line planting of double hedgerows (3 m long plot, 1/2 meter interval, 45 plants in each plot) with 5 replicates. Plants were lopped every 5 months and samples (10 different plants within a plot; 4 newly flushed leaves, 4 partially expanded leaves and 4 mature full expanded leaves from each of 10 trees in a plot) were collected and were put on ice before transfer to the laboratory. Proanthocyanidine was extracted in aqueous acetone, and total extractable proanthcyanidine (TEPA) and total proanthocyanidine (TOPA) were measured. In addition, proximate analyses were made of different provenances. Crude protein contents varied from 19.74% to 16.45%; in vitro organic matter digestibility ranged from 36.09% to 23.32%. TEPA content ranged from 11.22 to 16.01 (measured as absorbance at $550nm,\;g^{-1}$ DM) and TOPA from 24.57 to 31.72 (measured as absorbance at $550nm,\;g^{-1}$ DM) TEPA and TOPA had a positive correlation, whereas crude protein content of Calliandra was negatively correlated with TEPA and TOPA. Provenances, such as Georgesville (Belize), local, Coban (Guatemala), Patulul (Guatemala), Bombana (Mexico) can be selected on the basis of nutritive value of fodder.

• Asian-Australasian Journal of Animal Sciences
• /
• v.4 no.1
• /
• pp.31-40
• /
• 1991

Four pairs of draught oxen (two local and two Jersey crossbred) were studied when they ploughed dry land on local farms. Work done, distance traveled and body temperature of each ox were measured continuously over a 5 h working day. A different team worked each day, completing at least six days work each. Individual food intakes and digestibility of feed were measured when the animals were given rice straw and tree fodder, and housed and fed according to local husbandry practices. The Jersey crossbreds, particularly the longer legged type, had a higher rate of work than the local oxen in this study. They did significantly more work and covered a greater distance during the day. The absence of a hump in the crossbred oxen had no effect on the position of the yoke or the way the oxen pulled when ploughing. The longer legged type of Jersey crossbred tended to work more erratically than any of the other teams. A fast rate of work made the oxen more liable to heat stress. When fed according to local practices and given the same amount of feed as local oxen, Jersey crossbreds tended to do less well. During the ploughing months, the local oxen gained weight, while the crossbreds remained at the same or lost some weight. Although there were some disadvantages to keeping Jersey crossbreds for work, their favourable work output suggests that the introduction of the Jersey crossbred in the hills of Nepal is unlikely to be detrimental to the performance of the work oxen population.

• Mycobiology
• /
• v.34 no.1
• /
• pp.14-21
• /
• 2006

Crude protein (CP) content of mechanically ground rice straw into small particles by an electric grinder and reducing value (RV) and soluble protein (SP) in the culture filtrate were lower than that of the chopped straw into $5{\sim}6\;cm$ lengths when both ground and chopped straws were fermented with Aspergillus ochraceus, A. terreus or Trichoderma koningii, at steady conditions. The reduction rate of RV, SP and CP was 22.2, 2.4, 7.3%; 9.1, 4.9, 8.5% or 0.0, 0.0, 3.6% for the three fungi, respectively. Chemical pretreatment of straw by soaking in $NH_{4}OH$ for a day caused significant increase in CP of the fermented straw than the other alkali and acidic pretreatments. Gamma irradiation pretreatment of dry and wet straw with water, specially at higher doses, 100, 200 or 500 kGy, caused significant increase in RV and SP as CP in the fermented straw by any of these fungi. Chemical-physical combination pretreatment of rice straw reduced the applied dose of gamma irradiation required for increasing fermentable ability of fungi from 500 kGy to 10 kGy with approximately the same results. Significant increases in RV and SP of fermented straw generally occurred as the dose of gamma irradiation for pretreated straw, which combined with $NH_{4}OH$, gradually rose. Whereas, the increase percentage in CP of fermented straw that was pretreated by $NH_{4}OH-10\;kGy$ was 12.4%, 15.4% or 8.6% for A. ochraceus, A. terreus or T. koningii, respectively.