• Title/Summary/Keyword: flux control coefficient

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In Silico Analysis of Lactic Acid Secretion Metabolism through the Top-down Approach: Effect of Grouping in Enzyme kinetics

  • Jin, Jong-Hwa;Lee, Jin-Won
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.5
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    • pp.462-469
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    • 2005
  • A top-down approach is known to be a useful and effective technique for the design and analysis of metabolic systems. In this Study, we have constructed a grouped metabolic network for Lactococcus lactis under aerobic conditions using grouped enzyme kinetics. To test the usefulness of grouping work, a non-grouped system and grouped systems were compared quantitatively with each other. Here, grouped Systems were designed as two groups according to the extent of grouping. The overall simulated flux values in grouped and non-grouped models had pretty similar distribution trends, but the details on flux ratio at the pyruvate branch point showed a little difference. This result indicates that our grouping technique can be used as a good model for complicated metabolic networks, however, for detailed analysis of metabolic network, a more robust mechanism Should be considered. In addition to the data for the pyruvate branch point analysis, Some major flux control coefficients were obtained in this research.

Dynamic Modeling of Lactic Acid Fermentation Metabolism with Lactococcus lactis

  • Oh, Euh-Lim;Lu, Mingshou;Choi, Woo-Joo;Park, Chang-Hun;Oh, Han-Bin;Lee, Sang-Yup;Lee, Jin-Won
    • Journal of Microbiology and Biotechnology
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    • v.21 no.2
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    • pp.162-169
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    • 2011
  • A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).

Analysis of Orbital Lifetime Prediction Parameters in Preparation for Post-Mission Disposal

  • Choi, Ha-Yeon;Kim, Hae-Dong;Seong, Jae-Dong
    • Journal of Astronomy and Space Sciences
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    • v.32 no.4
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    • pp.367-377
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    • 2015
  • Atmospheric drag force is an important source of perturbation of Low Earth Orbit (LEO) orbit satellites, and solar activity is a major factor for changes in atmospheric density. In particular, the orbital lifetime of a satellite varies with changes in solar activity, so care must be taken in predicting the remaining orbital lifetime during preparation for post-mission disposal. In this paper, the System Tool Kit (STK$^{(R)}$) Long-term Orbit Propagator is used to analyze the changes in orbital lifetime predictions with respect to solar activity. In addition, the STK$^{(R)}$ Lifetime tool is used to analyze the change in orbital lifetime with respect to solar flux data generation, which is needed for the orbital lifetime calculation, and its control on the drag coefficient control. Analysis showed that the application of the most recent solar flux file within the Lifetime tool gives a predicted trend that is closest to the actual orbit. We also examine the effect of the drag coefficient, by performing a comparative analysis between varying and constant coefficients in terms of solar activity intensities.

Direct Thrust Control of Permanent Magnet Type Linear Synchronous Motor by using Digital Signal Processor (DSP를 이용한 영구 자석형 선형 동기전동기의 직접 추력 제어)

  • U, Gyeong-Il;Kim, Deok-Jin;Gwon, Byeong-Il
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.49 no.8
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    • pp.514-521
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    • 2000
  • This paper presents a direct thrust control scheme for permanent magnet linear synchronous motor(PMLSM) by using digital signal processor(DSP). And a simulation method for the direct thrust control of a permanent magnet linear synchronous motor using the equivalent circuit is presented. The detent force that was obtained by cubic spline method is considered in the simulation. Thrust correction coefficient is utilized to estimate actual thrust on the direct thrust control, which considers the longitudinal end effect due to the finite core length of the permanent magnet linear synchronous motor. The motor self inductance, the initial flux linkage by the permanent magnet is calculated in advance by the finite element analysis, and then the direct control simulation is carried out. As the results, thrust, current and speed are shwon.

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Study on the mixing performance of mixing vane grids and mixing coefficient by CFD and subchannel analysis code in a 5×5 rod bundle

  • Bin Han ;Xiaoliang Zhu;Bao-Wen Yang;Aiguo Liu;Yanyan Xi ;Lei Liu ;Shenghui Liu;Junlin Huang
    • Nuclear Engineering and Technology
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    • v.55 no.10
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    • pp.3775-3786
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    • 2023
  • Mixing Vane Grid (MVG) is one of the most important structures in fuel assembly due to its high performance in mixing the coolant and ultimately increasing Critical Heat Flux (CHF), which avoids the temperature rising suddenly of fuel rods. To evaluate the mixing performance of the MVG, a Total Diffusion Coefficient (TDC) mixing coefficient is defined in the subchannel analysis code. Conventionally, the TDC of the spacer grid is obtained from the combination of experiments and subchannel analysis. However, the processing of obtaining and determine a reasonable TDC is much challenging, it is affected by boundary conditions and MVG geometries. In is difficult to perform all the large and costing rod bundle tests. In this paper, the CFD method was applied in TDC analysis. A typical 5 × 5 MVG was simulated and validated to estimate the mixing performance of the MVG. The subchannel code was used to calculate the TDC. Firstly, the CFD method was validated from the aspect of pressure drop and lateral temperature distribution in the subchannels. Then the effect of boundary conditions including the inlet temperature, inlet velocities, heat flux ratio between hot and cold rods and the arrangement of hot and cold rods on MVG mixing and TDC were studied. The geometric effects on mixing are also carried out in this paper. The effect of vane pattern on mixing was investigated to determine which one is the best to represent the grid's mixing performance.

Gas sparged gel layer controlled cross flow ultrafiltration: A model for stratified flow regime and its validity

  • Khetan, Vivek;Srivastava, Ashish;De, Sirshendu
    • Membrane and Water Treatment
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    • v.3 no.3
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    • pp.151-168
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    • 2012
  • Gas sparging is one of the techniques used to control the concentration polarization during ultrafiltration. In this work, the effects of gas sparging in stratified flow regime were investigated during gel layer controlling cross flow ultrafiltration in a rectangular channel. Synthetic solution of pectin was used as the gel forming solute. The liquid and gas flow rates were selected such that a stratified flow regime was prevalent in the channel. A mass transfer model was developed for this system to quantify the effects of gas flow rates on mass transfer coefficient (Sherwood number). The results were compared with the case of no gas sparging. Gas sparging led to an increase of mass transfer coefficient by about 23% in this case. The limitation of the developed model was also evaluated and it was observed that beyond a gas flow rate of 20 l/h, the model was unable to explain the experimental observation, i.e., the decrease in permeate flux with flow rate.

A Study on Electric Safety Control Device for Prevention of Over Current and Short Circuit Faults (과전류 및 단락사고 방지용 전기안전 제어장치에 관한 연구)

  • Jo, Si-Hwan;Kwak, Dong-Kurl;Jung, Do-Young;Shim, Jae-Sun;Kim, Jung-Sook
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.2100-2101
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    • 2008
  • This paper is studied on a protective control system for electrical fire and electrical faults due to over current or electric short circuit faults by using electrical thermal characteristics of PTC (Positive Temperature Coefficient) thermistor and current response characteristics of high sensitive reed switch. The PTC thermistor has characteristic of positive resistivity temperature coefficient according to the temperature variation, which is construction of a regular square and cube demarcation with BaTiO3_Ceramics of positive temperature coefficient. Also PTC thermistor shows the phenomenon which is rapidly increased in the resistivity if the temperature is increased over Curie temperature point, and reed switch, which is used for electrical fault current sensing devices, have a excellent characteristic of response velocity in degree of ${\mu}s{\sim}ms$ that sensing magnetic flux in proportion to dimension of line current. This paper is proposed on a protective control system use PTC thermistor and reed switch for sensor which is protected from electrical fire due to overload faults or electric short circuit faults. Some experimental results of the proposed electric safety control device are confirmed to the validity of the analytical results.

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Processing and Quality Control of Big Data from Korean SPAR (Soil-Plant-Atmosphere-Research) System (한국형 SPAR(Soil-Plant-Atmosphere-Research) 시스템에서 대용량 관측 자료의 처리 및 품질관리)

  • Sang, Wan-Gyu;Kim, Jun-Hwan;Shin, Pyong;Baek, Jae-Kyeong;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.4
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    • pp.340-345
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    • 2020
  • In this study, we developed the quality control and assurance method of measurement data of SPAR (Soil-Plant-Atmosphere-Research) system, a climate change research facility, for the first time. It was found that the precise processing of CO2 flux data among many observations were sig nificantly important to increase the accuracy of canopy photosynthesis measurements in the SPAR system. The collected raw CO2 flux data should first be removed error and missing data and then replaced with estimated data according to photosynthetic lig ht response curve model. Comparing the correlation between cumulative net assimilation and soybean biomass, the quality control and assurance of the raw CO2 flux data showed an improved effect on canopy photosynthesis evaluation by increasing the coefficient of determination (R2) and lowering the root mean square error (RMSE). These data processing methods are expected to be usefully applied to the development of crop growth model using SPAR system.

Quantitative Study of CO2 based on Satellite Image for Carbon Budget on Flux Tower Watersheds (플럭스 타워 설치 유역을 대상으로 탄소수지 분석을 위한 위성영상자료기반의 CO2 정량화 연구)

  • Jung, Chung Gil;Lee, Yong Gwan;Kim, Seong Joon;Jang, Cheol Hee
    • Journal of The Korean Society of Agricultural Engineers
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    • v.57 no.3
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    • pp.109-120
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    • 2015
  • Spatial heterogeneous characteristics of solar radiation energy from Climate Change gives rise to energy imbalance in the general ecological system including water resources. This study is to estimate the $CO_2$ flux of South Korea using Terra MODIS image and to assess the reliability of MODIS data from the ground measured $CO_2$ flux by eddy covariance flux tower data at 3 locations (two at mixed forest area and one at rice paddy area). The MODIS Gross Primary Productivity (GPP) product (MOD17A2), 8-day composite at 1-km spatial resolution was adopted for the spatial $CO_2$ flux generation. The MOD17A2 data by noise like cloud and snow in a day were tried to fill by Inverse Distance Weighted (IDW) method from valid pixels and the damping effect of MOD17A2 data were corrected by Quality Control (QC) flag. The MODIS $CO_2$ flux was estimated as the sum of GPP and Re (ecosystem respiration) by Lloyd and Taylor method (1994). The determination coefficient ($R^2$) between MODIS $CO_2$ and flux tower $CO_2$ for 3 years (2011~2013) showed 0.55 and 0.60 in 2 mixed forests and 0.56 in rice paddy respectively. The $CO_2$ flux generally fluctuated showing minus values during summer rainy season (from July to August) and maintaining plus values for other periods. The MODIS $CO_2$ flux can be a useful information for extensive area, for example, as a reliable indicator on ecological circulation system.

Estimation of Overall Heat Transfer Coefficient for Single Layer Covering in Greenhouse (일중 피복온실의 관류열전달계수 산정)

  • Hwang, Young-Yun;Lee, Jong-Won;Lee, Hyun-Woo
    • Journal of Bio-Environment Control
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    • v.22 no.2
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    • pp.108-115
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    • 2013
  • This study was conducted to suggest a model to calculate the overall heat transfer coefficient of single layer covering for various greenhouse conditions. There was a strong correlation between cover surface temperature and inside air temperature of greenhouse. The equations to calculate the convective and radiative heat transfer coefficients proposed by Kittas were best fitted for calculation of the overall heat transfer coefficient. Because the coefficient of linear regression between the calculated and measured cover surface temperature was founded to 0.98, the slope of the straight line is 1.009 and the intercept is 0.001, the calculation model of overall heat transfer coefficient proposed by this study is acceptable. The convective heat transfer between the inner cover surface and the inside air was greater than the radiative heat transfer, and the difference increased as the wind speed rose. The convective heat transfer between the outer cover surface and the outside air was less than the radiative heat transfer for the low wind speed, but greater than for the high wind speed. The outer cover convective heat flux increased proportion to the inner cover convective heat flux linearly. The overall heat transfer coefficient increased but the cover surface temperature decreased as the wind speed increased, and the regression function was founded to be logarithmic and power function, respectively.