• Journal of Embryo Transfer
• /
• v.9 no.2
• /
• pp.153-160
• /
• 1994

This study evaluated the influence of cell stage of donor nucleus on nuclear injection, electrofusion and in vitro development in the rabbit to improve the efficiency of nuclear transplantation in the rabbit. The embryos of 8-, 16- and 32-cell stage were collected from the mated does by flushing viducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FGS) at 44, 54 and 60 hours after hCG injection. The blastorneres separated from these embryos were used as donor nucleus. The ovulated oocytes collected at 14 hours after hCG injection were used as recipient cytoplasm following removing the nucleus and the first polar body. The separated blastomeres were injected into the enucleated oocytes by micromanipulation and were electrofused in 0.28 M mannitol solution at 1.5 kV /cm, 60 $\mu$sec for three times. The fused oocytes were cocultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FGS for 72~120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The cultured nuclear transplant embryos were stained with Hoechst 33342 solution and the number of cells were counted by fluorescence microscopy. The successful injection rate of 8-, 16- and 32-cell-stageblastomeres into enucleated oocytes was 86.7, 91.0 and 93.9%, respectively. The electrofusion rate of 8-, 16- and 32-cell-stage blastomeres with enucleated oocytes was 93.3,89.3 and 79.0%, respectively. Development of blastomeres to blastocyst was similar with 8-,16- and 32-cell-stage donor nuclei(26.2, 25.8 and 26.6%, respectively, P<0.05). The mean number of cell cycle per day during in vitro culture in nuclear transplant embryos which received 8-, 16- and 32-cell- stage nuclei was 1.87, 1.81 and 1.43, respectively.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.47 no.6
• /
• pp.83-92
• /
• 2005

Natural environment of the Wolgokri stream watershed, located in Chuncheon, Gangwon province, Korea, has been well preserved as a traditional agricultural watershed. To analyze characteristics of NPS pollution generated from an mountainous agricultural watershed, the flow and water qualities of the study watershed were monitored and were analyzed to estimate pollution loads. Annual runoff volume ratio was $70.4\%$. Concentrations of T-N, T-p, COD, and TOC were higher when monthly rainfall was between $0\~30mm$ than those when monthly rainfall was between $30\~70mm$. However, the concentrations varied considerably when monthly rainfall was higher than 100mm. The flow weighted mean concentrations(mg/L) of BOD, COD, TOC, $NO_3-N$, T-N, T-P and SS were 1.96, 2.72, 3.32, 1.41, 4.70, 0.187 and 13.36, respectively. The BOD, SS, T-N and T-P loads of July, 2004 were $48\%,\;17\%,\;51\%\;and\;32\%$ of annual load, respectively. The BOD, COD, TOC, $NO_3-N$, T-N, T-p, and SS loads (kg/ha) from Mar. 2004 to Apr. 2005 were 19.09, 26.55, 32.39, 13.85, 45.92, 1.887 and 130.18, respectively. The highest concentrations of BOD, NO3-N, T-N, T-p, SS, COD and TOC were found before the flow reached the peak runoff, possibly due to the first flushing effect. Generally, pollution loads of the Wolgokri watershed were not that significant. Phosphorus load, however, was higher enough to cause eutrophication in the receiving water body It was recommended that best management practices need to be implemented to reduce phosphorus sources.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.21 no.3
• /
• pp.230-240
• /
• 2009

The ESCORT model is applied at Mokpo coastal zone to analyze the effect of fresh water discharge released from the gates of the Youngsan River sea-dyke. Applicability and validity of both 2-D and 3-D hydrodynamic modules could be guaranteed by simulating hydrodynamic feature with ebb dominance characteristics. Then, effects of the released discharge on the hydrodynamics are investigated. And analysis of the effects on diffusion phenomena show that 3-D model is essential for such diffusion modelling. Moreover, vertical salinity distributions near the gates are examined, and the influence range of fresh water is also estimated, which shows that dilution of fresh water is going on slowly because of poor flushing.

• Korean Journal of Digital Imaging in Medicine
• /
• v.16 no.1
• /
• pp.27-33
• /
• 2014

The purpose of this study evaluated the hemo-dynamic information within 30 seconds clinically in 3D breast MRI. From January to March 2014, A total of 40 people were examined at 1.5 Tesla(Philips, Medical System, Achieva, The Netherlands) MRI equipments using 16 channel SENSE breast coil. The imaging parameters on vibrant are fellow as: $TR/TE/FA^{\circ}$/Matrix size/Slice thickness/Slab($5ms/2ms/10^{\circ}/180{\times}139{\times}2mm/80$). This study used a Gadovist and injected it with injection speed of 4 ml /sec by auto injector with 15 ml saline flushing. Firstly, for the delay time study, it divided three different delay time from immediately, 20 seconds, and 30 seconds. In quantitative analysis, the ROI signal intensities of tumor and surrounding tissues were measured retrospectively. In qualitative analysis, the image quality was scored from 1 to 5 point by one experienced radiological technologists as a visual test. The significance level of each delay time was evaluated with a one-way ANOVA(p<0.05). In the visual test, score levels on 30 seconds delay time was a little bit higher than others(p<0.05). The signal intensity of the tumor were $1445{\pm}360$, $1410{\pm}320$, $1510{\pm}415$ on immediately, 20 seconds, and 30 seconds and score levels were $4.18{\pm}0.85$, $3.54{\pm}0.94$, $4.45{\pm}0.74$(p<0.05). The data on immediate images showed better results than that others(p<0.05). Conclusively, Although it has been high scored in 30sec delay time for visual test in order to avoid failure in 20second, 30seconds delay time after contrast media administration, we recommend that the DCE 3D breast MRI commence immediately.

• Korean Journal of Animal Reproduction
• /
• v.13 no.3
• /
• pp.179-187
• /
• 1989

The experiment was carried out to study the profile of uterine specific protein during early pregnancy in sows and to test it's immunosuppressive activity. Uterine protein samples were obtained by flushing the uterine horn on Day 3, 6, 9, 12 and 15 of the estrous cycle and the pregnancy respectively and the protein concentration of each sample was determined. The change of uterine protein was examined by sodium dodecyl sulfate(SDS)-PAGE. The immunosuppressive activity of uterine secretory protein was investigated according to the lymphocyte blastogenesis response to mitogen. The results of this experiment are summarized as follows ; 1. The uterine protein during estrous cycle and early pregnancy was relatively constant up to Day 9, but increased on Day 12. Maxium total protein values were found on Day 15. The concentration of serum proteins were about 82-95 mg during estrous cycle, but decreased to about 70-82 mg during early pregnancy. 2. The proteins components similar electrophoretic patterns(PAGE) that were no differences (band ; a, b, c, d, e, f, g, I) on Days 3, 6 and 9 of the estrous cycle and pregnancy. But there were more 2 bands specifically on Day 12 of the pregnancy and on Day 15 of estrous cycle and showed more 4 bands on Day 15 of early pregnancy. They seemed to be acidoprotein and their average molecular weight were 38,000, 22,300 and 12,600. 3. When uterine protein were added 500$\mu\textrm{g}$/ml, there was no immunosuppresive activity on Day 3 of estrous cycle and lymphocyte blastogenesis was slightly suppressed on Day 3 of pregnancy. The immunosuppressive activity on Day 9 of estrous cycle and pregnancy appeared in 500$\mu\textrm{g}$/ml and 150$\mu\textrm{g}$/ml, respectively the uterine protein on Day 12 and 15 showed immunosuppresive activity, which at the level of 150$\mu\textrm{g}$/ml during non-pregnancy and at the level of 100 to 125$\mu\textrm{g}$/ml during early pregnancy, respectively.

• Korean Journal of Ecology and Environment
• /
• v.42 no.4
• /
• pp.452-464
• /
• 2009

The objectives of this study were to evaluate spatial and temporal variability of water quality in 10 reservoirs and identify the key nutrients (N, P) influencing chlorophyll-a (CHL) along with analysis of empirical models and zonal patterns of total phosphorus (TP) and CHL. We analyzed total nitrogen (TN), TP, CHL, water clarity (Secchi depth, SD), and evaluated potential limiting nutrient using ambient N:P ratios and previous criteria of ambient nutrients. Water clarity and CHL varied largely depending on the seasonal monsoon and type of reservoir, but trophic state was diagnosed as eutrophy, base on mean CHL in most reservoirs. The peak of TP did not match the contents of CHL due to rapid flushing during the high run-off period. In the reservoir of DR, regression coefficient in the $P_r$ was 0.510 but was 0.159 in the $M_o$, while the TP-CHL relation in the YR increased during the monsoon compared to the premonsoon. The regression coefficient in the $P_r$ was not statistically significant but the value of $M_o$ was 0.250. TP showed similar longitudinal zonal gradients among the reservoirs of DR, YR and JR. Empirical models of TP-CHL, based on overall data, showed that CHL was determined by phosphorus($R^2=0.244$, p=0.0019). Regression analysis of CHL-SD showed a stronger linear fit ($R^2=0.638$, p<0.001) than the TP-CHL model.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.56 no.2
• /
• pp.114-125
• /
• 2020

In order to understand the currents around Hampyung Bay and Haeje Peninsula, 2D numerical simulations for tidal currents and tide-induced residual currents were carried out. Dominant semidiurnal tidal currents have reversing form and flow NNE-SSW from northern Haeje Peninsula to Songi Island, E-S at northern Haeje Peninsula and NNW-SSE in Hampyung Bay. In flood, a part of currents from Imja Island~Nakwhol Island flow along the main stream flowing northeast at offshore region and the rest flow into Hampyung Bay flowing east along the northern coast of Haeje Peninsula. In ebb, currents from Hampyung Bay flow west along the northern coast of Haeje Peninsula and run together with the main stream flowing southeast at offshore region. The currents create an anticyclonic circulation in flood and a cyclonic circulation in ebb around Haeje Peninsula including Hampyung Bay. Tidal currents are accumulated on Doripo which located at the entrance of Hampyung Bay and show high current velocities. Tidal currents and tide induced residual currents are weak at the inside of Hampyung Bay which has narrow entrance, shallow water depth and wide intertidal zone. An anticyclonic eddy is formed around Gaksi Island as a result of tide induced residual currents. In northern coast of Haeje Peninsula, slow constant currents flow east. It is expected that a gradual change of sediment and an increase of flushing time for suspended materials are carried by tidal currents occurring in Hampyung Bay.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.31 no.4
• /
• pp.65-82
• /
• 2018

Objectives : The purpose of this study is 1) to explore the general tendency of the herbs or herbal formula for acne treatment in clinical practice 2) and to provide a basis for explaining the principles of acne treatment in Korean Medicine in order to make patients understand more easily based on pharmacological mechanism by analyzing the herbs or herbal formula using for acne treatment in clinical practice. Methods : In the domestic databases (Oriental medicine Advanced Searching Integrated System-OASIS, Korean Traditional Knowledge Portal-KTKP, National Discovery for Science Leader-NDSL, Research Information Sharing Service-RISS), we selected among the papers published using search terms related to "acne". Reports related to sasang constitutional medicine were excluded and a total of 23 papers were finally selected. Results : 29 prescriptions were retrieved from 23 papers. Chungan-tang (淸顔湯) notified formulas ranked first by Cheongsangbangpung- tang (淸上防風湯), Hwangnyeonhaedok-tang (黃連解毒湯), Dangguijakyaksan (當歸芍藥散) and Bipachungpe-eum (枇杷淸肺飮). Commonly used herbs are Forsythiae Fructus (連翹), Scutellariae Radix (黃芩), Gardeniae Fructus (梔子), Ligustici Rhizoma (川芎), and Angelicae Gigantis Radix (當歸). In most patients with inflammatory lesions or excessive flushing, exterior-releasing medicinal (解表藥) and heat-clearing medicinal (淸熱藥) were mainly prescribed for acne treatment, while in patients with acne accompanied hyperkeratinization, Excess sebum secretion, Dysmenorrhea or indigestion, qi-tonifying medicinal (補氣藥) or blood-tonifying medicinal (補血藥) were mainly used. KAGS (Korean Acne Grading System) was the most commonly used method for acne severity assessment. Conclusions : These findings suggest that the herbs or herbal formula for acne treatment in Korean Medicine is prescribed differently according to the cause and symptoms of acne in a unique way for control the whole body balance. Also, our findings could provide a basis for explaining the principles of acne treatment in Korean Medicine in order to make patients understand more easily based on pharmacological mechanism in clinical practice.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• v.11 no.2
• /
• pp.173-177
• /
• 2017

Bidet is widely used and is being developed for multipurpose. However, use of this bidet varies by the elderly and disabled people. Therefore, this research compares and analyze need of bidet for elderly and disabled people and by many different types. This survey was conducted targeting 39 elderly and 120 disabled people. This survey is consist of questions that varies by bidet companies and includes questions like UI cognitive abilities, automatic water flushing, position of control box and necessary of embossment for icons. As a result, elderly and disabled people showed similar in result but for visually impaired person, the ratio of preference showed 1:9 which is opposite. As for visually impaired person, the ratio of preference showed 1:9 which is pretty high.

• Clean Technology
• /
• v.17 no.4
• /
• pp.353-362
• /
• 2011

In our nature, the utilization of rainwater is essential for healthy water recirculation. This is one of the solutions of the increment of impermeability surface according to the development of new cities; this study of the improvement of rainwater quality has been carried on through the improvement of collecting and restoring system of rainwater. The southwestern region of Daejeon City, the rainwater coefficient of run off was 0.40 and this number had computed to 0.59 after the development. After filtration of rainwater, the heavy metal (Cu, As, Cr, Fe, Mn) contents level were lower than underground water. Moreover, collected rainwater showed better quality than underground water in following criteria; hardness, permanganate consumption quality, chloride, evaporation residue, sulfates and nitrate nitrogen. This water quality met the gray water quality standards. The rainwater quality was still suitable to use as bathroom flushing and gardening after 100 days of storage. This study proved that modification (installation of cover with gutter to existing rainwater collection system, proper filtering, and installation of underground storage tank) of collection system could improve quality of water and maintain this approximately 100 days.