• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.783-788
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• 2002

Bovine yolk sac at day 24 of pregnancy, and placental membranes (chorion and allantois) from days 70 and 100 of pregnancy were isolated and cultured in a modified minimum essential medium in the presence of $[^{35}S]$methionine. Proteins synthesized and secreted by isolated bovine yolk sac, chorion and allantois were analyzed by fluorography of two-dimensional polyacrylamide gel electrophoresis. Serum-like proteins,transferrin, ${\alpha}$-fetoprotein, ${\alpha}$1-antitrypsin and ${\alpha}$1-acid glycoprotein,were the major protein products of yolk sac. A 21 kDa protein produced by yolk sac was identified immunochemically as retinol-binding protein (RBP). Chorion and allantios from days 70 and 100 of pregnancy were active in protein synthesis and secretion. Both chorion and allantois did not secret serum-like proteins but secreted a number of neutral-to-acidic proteins including RBP. Secretory proteins produced by the yolk sac, chorion and allantois may play important roles in the embryonic development and the successful outcome of pregnancy. Antiserum against bovine placental RBP was employed to the immunocytochemistry by immunoperoxidase method. Immunoreactive RBP was localized in epithelial cells and island-like cell clones of yolk sac. Immunostaining for RBP was detected in simple columnar epithelium of chorion and in simple squamous epithelium of allantois. In the present study, proteins synthesized and secreted by yolk sac at day 24 of pregnancy, chorion and allantois from days 70 and 100 of pregnancy were characterized In addition, RBP was localized in yolk sac, chorion and allantois by immunoperoxidase method. The immunoperoxidase method has been proven to be a very effective technique to identify the cellular source of protein synthesis in extraembryonic membranes.

• Korean Journal of Digital Imaging in Medicine
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• v.14 no.2
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• pp.47-56
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• 2012

Amount of radiation exposure by seeing through fluoroscopy examination while is many patient exposure administration and unprepared misgovernment be. In this study, abdominal fluoroscopy during the scan, the dose and image quality change according to the use of grid and added filter optimized by measuring the test condition is proposed. Uses seeing through fluoroscopy examination equipment of Image Intensifier of Easy Diagnost Eleva (Philips), under tube type and uses Human phantom and measures average area dose according to grid insertion existence and nonexistence and added filter kind change. Measure sum of 29 organ dose and effective dose through PCXMC imagination simulation program and image J program through noise, SNR, image distortion was measured. Resolution, sharpness, and analyzed using the MTF curves. Fluorography the grid to insert the filter thickness and thickening and increased the average area dose and organ doses and effective dose. In the case of spot examination, when inserted grid, average area dose and organ dose and effective dose increased. Filter thickens the average area dose decreased, but the organ doses and effective dose were increased when use 0.2mmCu+1mmAl filter, decreased slightly. Noise and SNR measurements without inserting the gird, if you do not use the added filter was the lowest and when measure the distortion, 0.1mmCu+1mmAl filter was no difference of image quality in case insert grid was judged that when did not use occasion added filter that do not use grid, difference of image quality does not exist. Did not show a big difference, according to the grid and uses of the added filter sharpness, and resolution. Patient dose increases with factors that reduce the quality of the image so reckless grid and the use of the added filter when abdominal fluoroscopy examination should be cautious in using.

• The Korean Journal of Zoology
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• v.38 no.4
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• pp.490-497
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• 1995

In vitro tissue culture of fat body of Hyphantria cunea in the medium containing [35S]-methionine reveaied that storage protein-i (SP-1) is taken up into fat body of prepupae and 1-day-old pupae. Using Western blotting and ligand binding method, we were able to identify the protein band of the SP-1 receptor protein. For the partial purification, the membrane proteins of fat body cells were solubilixed with 1% Triton X-1OO and applied to anion exchange chromatography. The results revealed the molecular weight of the receptor protein to be about 80 kl)a in SDSPAGE, and the P1 was estimated to be about 6.1. The mobility of the receptor protein in 8D8-PAGE was highly dependent on both temperature during electrophoresls and the condition of samples whether they were in reducing or nonreducing.

• Journal of radiological science and technology
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• v.25 no.2
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• pp.71-75
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• 2002

According to this study, we can make the radiation check meter which have not supply because of high cost and import barrier and lengthen its life by means of repairing of radiation bomb and equipment. We can make better medical service. In my study, I used the photodiod, photoelectron, among semiconductor detectors which have a excellent detect capacity and are low cost and small size. I set up this equipment in June 1, 2002, used 640 mA remote operative fluorography equipment, which make the grade as capacity test. I used the standard measuring instrument which took proofs from a agency, now it was using in measuring agency. The comparative measuring instrument used in same condition. I took the standard which was gauged with a connecting measuring instrument. Using a existing unconnected measuring instrument, I compared the accuracy with new unconnected one. As a result, three score are within the standard. For the detailed analysis, I took the average of percentage average error. So standard instrument was -0.02, comparable was -0.22, and new one was -0.17. New one took a closer measured value with standard than comparable one. In more study, I think to take more accurate value. I expect that my study will be a base of measuring instrument, with low cost, supply of this instrument increase, I expect to decrease radiation bomb and maintain, repair and manager better.