• Journal of Environmental Health Sciences
• /
• v.38 no.2
• /
• pp.136-141
• /
• 2012

Objectives: The objective of this study was to investigate whether the ammonia nitrogen concentration of aqueous samples such as drinking water can be determined by measuring the saturation of the samples colored by indophenol method. Methods: A color saturation measurement system was constructed by connecting a notebook computer to an image acquisition device composed of a PC camera and a light source, and was then used to measure the saturation of samples colored by blue indophenol complex. Results: Between two available light sources, a fluorescent lamp was selected due to its demonstrating better linearity between color saturation and ammonia nitrogen concentration. Prediction by quadratic regression was more accurate than by linear regression, and prediction by quadratic regression in the concentration range of 0.1-1.0 $mg/l$ was more accurate than in the concentration range of 0.0-1.0 $mg/l$. Regression-based predictions over 0.25 $mg/l$, 0.55 $mg/l$ and 0.75 $mg/l$ concentrations were implemented both by spectrophotometric method and by measuring color saturation. In the case of 0.25 $mg/l$, the predicted concentration by spectrophotometric method was $0.256{\pm}0.0076\;mg/l$ and the predicted concentration by measuring color saturation was $0.246{\pm}0.0086\;mg/l$ (p=0.051). In the case of 0.55 $mg/l$, they were $0.561{\pm}0.0068\;mg/l$ and $0.564{\pm}0.0166\;mg/l$ (p=0.660). In the case of 0.75 $mg/l$, they were $0.755{\pm}0.0139\;mg/l$ and $0.762{\pm}0.0088\;mg/l$ (p=0.215). Conclusions: There were no statistically significant differences (p>0.05) between the data from the two methods in all three of the concentrations. Therefore, the color saturation measurement method proposed in this paper may be considered applicable for determining the ammonia nitrogen concentration of aqueous samples such as drinking water.

• Journal of Korea Society of Industrial Information Systems
• /
• v.20 no.3
• /
• pp.19-28
• /
• 2015

With the aim of developing a smartphone-based point-of-care device that is small, inexpensive, and easy to handle by non-expert, we designed a fluorescence smartscope for counting particles and a DC motor-controlled particle positioning system. Our smartscope can count the number of fluorescent particles and fluorescently-stained white blood cells through a phone camera with an adaptor containing a LED, a ball lens and optical filters and an application running on a smartphone. The motor was controlled wirelessly via Bluetooth with an Android smartphone. We found that axial spinning of a helical microfluidic channel allows arrangement of particles having size similar to the white blood cells. The motor-controlled particle positioning system can minimize time-consuming manual processes and automate sample preparation process and thus, if integrated with the smartscope, it can be used for a point-of-care testing device based on a smartphone.

• Journal of Pharmacopuncture
• /
• v.8 no.3
• /
• pp.5-9
• /
• 2005

Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.

• Journal of the Korean Society of Costume
• /
• v.58 no.4
• /
• pp.113-127
• /
• 2008

Artificial lights have effected the changes of art and fashion concepts as well as human life since the invention of electric light bulb in late 19th century. Artist and designer have had more interested in these artificial lights as the development of digital technology and the change of millennium and they have tried to apply the lights into their works. The purpose of this study is to analyze the aesthetic characteristics of contemporary fashion design using artificial light as a medium. Artificial light for fashion design means the light using luminescent material like phosphorescent and fluorescent materials or in combination with electroluminescent digital technology or the light that can be perceived as images when light projects from media using a light projector or other digital equipment. Fashion design using this light type can change colors or form temporarily and it can playa role as a gadget for hm or as equipment to provide information much as a computer monitor does. And designer can create virtual patterns on the surface of clothes, or virtual fashion like a 3-dimensional holography in empty space. In these fashion designs, the virtual image of light is substituted for physical formative elements in fashion, and the viewer can experience an ambiguity between reality and virtuality. The results of the study were as follow; The formative characteristics of those fashion designs were identified as visibility, indeterminacy, integration and virtuality. And they reflected the internal meanings; the persue of protection and safety, the search for experiment and innovation, the will for interaction and communication and the desire for the deviation and fun.

• Fashion & Textile Research Journal
• /
• v.15 no.6
• /
• pp.862-873
• /
• 2013

The purpose of this study is to analyze the formative characteristics and the aesthetic values of the Military style in the 20th century and the images of the Military look shown in recent collections. The results of this study are as follows; 1) The aesthetic values of the Military style includes authority, functionality, resistibility and bisexuality. Authority is represented in suits and coats attaching details such as epaulets, flap pockets, gold buttons and badges. Functionality is represented in pants suits and skirts suits which are comfortable and simple. Resistibility is represented in wrinkled, dirty-old and torn military items. Bisexuality is shown on the military pants suits that a skirt or a mink coat is draped over. 2) The Military look in collections are expressed in classic, romantic, sexual, sporty, avant-garde and ethnic images. Classic images are appeared in suits and coats attaching details such as epaulets, flap pockets, gold buttons and badges. Romantic images are represented in the military items made of pastel-tone lace or splendid colored silk. Sexual images are appeared in designs to expose or to focus in women's body. Sporty images are represented in the military items made of new materials such as cotton-fleece, cotton-jersey and tencel. They have camouflage prints, melange-gray and fluorescent colors. Avant-garde images are appeared in the deformed suits and outer made of special materials with camouflage prints, khaki, gray and black. Ethnic images are represented in wearing military and ethnic items at the same time or military items made of ethnic colors, prints and handmade details.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.2
• /
• pp.144-151
• /
• 2014

Increasing the gene copy number has been commonly used to enhance the protein expression level in the yeast Pichia pastoris. However, this method has been shown to be effective up to a certain gene copy number, and a further increase of gene dosage can result in a decrease of expression level. Evidences indicate the gene dosage effect is product-dependent, which needs to be determined when expressing a new protein. Here, we describe a direct detection of the gene dosage effect on protein secretion through expressing the enhanced green fluorescent protein (EGFP) gene under the direction of the ${\alpha}$-factor preprosequence in a panel of yeast clones carrying increasing copies of the EGFP gene (from one to six copies). Directly examined under fluorescence microscopy, we found relatively lower levels of EGFP were secreted into the culture medium at one copy and two copies, substantial improvement of secretion appeared at three copies, plateau happened at four and five copies, and an apparent decrease of secretion happened at six copies. The secretion of EGFP being limiting at four and five copies was due to abundant intracellular accumulation of proteins, observed from the fluorescence image of yeast and confirmed by western blotting, which significantly activated the unfolded protein response indicated by the up-regulation of the BiP (the KAR2 gene product) and the protein disulfide isomerase. This study implies that tagging a reporter like GFP to a specific protein would facilitate a direct and rapid determination of the optimal gene copy number for high-yield expression.

• Journal of Biosystems Engineering
• /
• v.26 no.6
• /
• pp.571-578
• /
• 2001

Production of green pepper has increased for ten years in Korea, as customer's preference of a pepper tuned to fiesta one. This study was conducted to develop an on-line fading algorithm of green pepper using machine vision and aimed to develop the automatic on-line grading and sorting system. The machine vision system was composed of a professive scan R7B CCD camera, a frame grabber and sets of 3-wave fluorescent lamps. The length and curvature, which were main quality factors of a green pepper were measured while removing the stem region. The first derivative of the thickness profile was used to remove the stem area of the segmented image of the pepper. A new boundary was generated after the stem was removed and a baseline of a pepper which was used for the curvature determination was also generated. The developed algorithm showed that the accuracy of the size measurement was 86.6% and the accuracy of the bent was 91.9%. Processing time spent far grading was around 0.17 sec per pepper.

• Proceedings of the KSAR Conference
• /
• 2001.03a
• /
• pp.41-41
• /
• 2001

Chromatin configuration and microtubule assembly were determined in porcine maturing and activated oocytes following intracytoplasmic sperm injection. Microtubule localization was confirmed using a mouse monoclonal antibody to $\alpha$-tubulin and detected using a fluorescent labeled goat anti-mouse secondary antibody. DNA was stained with propidium iodide. The image of microtubules and chromatin was captured using laser scanning confocal microscope. In germinal vesicle stage oocyte, sperm chromatin remained condensation and sperm derived microtubules were not observed at 8 to 12 h after sperm injection. At 24 h after injection, the sperm nucleus developed to the metaphase chromatin along the metaphase structure of female nucleus. In some metaphase I stage oocytes, sperm chromatin decondensed at 8 h to 12 h after injection, sperm aster was seen soon after sperm injection. At 24 h after sperm injection into metaphase I stage oocyte, male chromatin developed to the metaphase chromatin while female chromatin extruded first polar body and formed the metaphase chromatin. At 12 to 15 h after sperm injection into preactivated oocytes, condensed sperm nucleus was located in close proximity of female pronucleus. However, the condensed nucleus did not fuse with female pronucleus. In preactivated ocytes, injected sperm remained condensation, a few sperm organized small microtubular aster. Instead, maternal derived microtubules were organized near the female chromatin, which seem to move condensed male chromatin near to the female pronucleus. These results suggest that sperm nuclear decondensing activity and nucleation activity of centrosome during fertilization are cell cycle dependent. In absence of male functional centrosome, female origin centrosome takes over the role of microtubule nucleation for nuclear movement.

• The Journal of Korean Medicine
• /
• v.39 no.1
• /
• pp.35-43
• /
• 2018

Objectives: In the brain, glutamate is the most important excitable neurotransmitter in physiological and pathological conditions. However, the high level of glutamate induces neuronal cell death due to exitotoxicity and oxidative stress. The present study investigated to evaluate a possible neuroprotective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death. Methods: Furosin was isolated from methanol extract of Euphorbia helioscopia and examined whether it protects glutamate-induced neuronal cell death. The cell viability was determined using Ez-Cytox assay. Anti-oxidative effect of furosin was determined by DPPH scavenging activities, and the levels of intracellular reactive oxygen species (ROS) were determined by the fluorescent intensity after staining the cells with $H_2DCFDA$. To evaluate apoptotic cell death, we performed nuclear staining and image-based cytometeric analysis. Results: The cell viability was significantly increased by treatement with furosin compared with the treatment with glutamate. Furosin showed a strong DPPH radical scavenging activity ($EC50=1.83{\mu}M$) and prevented the accumulation of intra cellular ROS. Finally, the presence of 50 and $100{\mu}M$ furosin significantly the percentage of apoptotic cells compared with glutamate treatment. Conclusion: The present study found that furosin is a potent neuroprotectant against glutamate-induced oxidative stress through inhibition of apoptotic cell death induced by glutamate. Therefore, the present study suggests that furosin as a bioactive compound of E. helioscopia can be a useful source to develop a drug for the treatment of neurodegenerative diseases and acute brain injuries.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.56 no.12
• /
• pp.2208-2213
• /
• 2007

In this study we have developed a hyperspectrum imaging system for highly sensitive and effective imaging analysis. An optical setup was designed using acoustic optical tunable filter (AOTF) for high sensitive hyperspectrum imaging. Light emitted by mercury lamp gets split in to diffracted and undiffracted beams while passing though AOTF. GFP transfected HEK-293 cell line was used as a model for in vitro imaging analysis. Cells were first, analyzed by fluorescence microscope followed by flow cytometric analysis. Flow cytometric analysis showed 66.31% transfection yield in GFP transfected HEK-293 cells. Various images of GFP transfected HEK-293 cell were grabbed by collecting the diffracted light using a CCD over a dynamic range of frequency of 129-171 MHz with an interval of 3 MHz. Subsequently, for in vivo image analysis of GFP transfected cells in mouse, a whole-body-imaging system was constructed. The blue light of 488 nm wavelength was obtained from a Xenon arc lamp using an appropriate filter and transmitted through an optical cable to a ring illuminator. To check the efficacy of the newly developed whole-body-imaging system, a comparative imaging analysis was performed on a normal mouse in presence and absence of Xenon arc irradiation. The developed hyperspectrum imaging analysis with AOTF showed the highest intensity of green fluorescent protein at 153 MHz of frequency and 494 nm of wavelength. However, the fluorescence intensity remained same as that of the background below 138 MHz (475 nm) and above 162 MHz (532 nm). The mouse images captured using the constructed whole-body-imaging system appeared monochromatic in absence of Xenon arc irradiation and blue when irradiated with Xenon arc lamp. Nevertheless, in either case mouse images appeared clearly.