• Nuclear Medicine and Molecular Imaging
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• v.41 no.3
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• pp.226-233
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• 2007

Purpose: Dual reporter gene imaging has several advantages for more sophisticated molecular imaging studies such as gene therapy monitoring. Herein, we have constructed hepatoma cell line expressing dual reporter genes of sodium iodide symporter (NIS) and enhanced green fluorescence protein (EGFP), and the functionalities of the genes were evaluated in vivo by nuclear and optical imaging. Materials and Methods: A pRetro-PN vector was constructed after separating NIS gene from pcDNA-NIS. RSV-EGFP-WPRE fragment separated from pLNRGW was cloned into pRetro-PN vector. The final vector expressing dual reporter genes was named pRetro-PNRGW. A human hepatoma (HepG2) cells were transfected by the retrovirus containing NIS and EGFP gene (HepG2-NE). Expression of NIS gene was confirmed by RT-PCR, radioiodine uptake and efflux studies. Expression of EGFP was confirmed by RT-PCR and fluorescence microscope. The HepG2 and HepG2-NE cells were implanted in shoulder and hindlimb of nude mice, then fluorescence image, gamma camera image and I-124 microPET image were undertaken. Results: The HepG2-NE cell was successfully constructed. RT-PCR showed NIS and EGFP mRNA expression. About 50% of cells showed fluorescence. The iodine uptake of NIS-expressed cells was about 9 times higher than control. In efflux study, $T_{1/2}$ of HepG2-NE cells was 9 min. HepG2-NE xenograft showed high signal-to-background fluorescent spots and higher iodine-uptake compared to those of HepG2 xenograft. Conclusion: A hepatoma cell line expressing NIS and EGFP dual reporter genes was successfully constructed and could be used as a potential either by therapeutic gene or imaging reporter gene.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2004.04a
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• pp.447-453
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• 2004

Various structural materials have been used in construction projects using stones, connotes, and steels materials. Among of these projects, concretes may use widely because concretes have high compressive strength, and comparatively easy maintenance and management. Reinforced concrete Buildings will be deteriorated as time passed. These problems will be accelerated by propagation of cracks. In order to manage such cracks, time, efforts and expense are required. In this study, leakages of fluorescence and adhesive material were investigated using glass sensors that were embedded in a model beam and column. In addition, currents in glass pipe sensor were observed to find leakage of liquid in glass pipes. Progressive cracks were generated by fracture of glass me sensor. In this investigation, a reinforcement clothing system was wrapped for a glass pipe sensor, The glass pipe sensor that can make control and reinforce cracks simultaneously.

• Journal of Advanced Marine Engineering and Technology
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• v.26 no.3
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• pp.362-374
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• 2002

For the purpose of development of a GDI engine, the in-cylinder phenomena, such as the spray behaviors, fuel distributions, unburned fuel, and flame characteristics were investigated in a single cylinder GDI engine. The GDI engine was equipped with a swirl type electronic injector and SCV(Swirl Control Valve). PLIF(Planar Laser Induced Fluorescence) system with KrF Excimer laser was used far the measurements of fuel distributions. The effects of the injector specifications, such as the spray cone angle and the offset angle on the in-cylinder phenomena were investigated. As a result, it was found that the injected fuel collided with the bottom of the bowl and moved upward along the exhaust side wall of piston bowl. This fuel vapor played an important role in the instance of spark ignition. The unburned fuel and flame characteristics were greatly influenced by the injector specifications.

• Journal of Environmental Impact Assessment
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• v.16 no.1
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• pp.15-26
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• 2007

Surface water is the main drinking water source in Korea. Algal bloom caused by phytoplankton in reservoir is common event in every summer season. To prevent or control the algal blooms, artificial circulation system has been adopted in many reservoirs, including Hoengseung reservoir. Total 7 hydraulic-gun-aerators were installed around the intake tower in Hoengseung reservoir since 2000. This study is to elucidate the effects of hydraulic-gun-aerators on phytoplankton bloom, pH, DO, temperature and evaluate the selective withdrawal and vertical distribution of phytoplankton by means of submersible fluorescence probe, which features high correlation with a standard ISO method (r=0.90, P<0.0001) for chlorophyll-a quantification.

• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.287-287
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• 2006

Amphiphilic random copolymers can form various types of micelles in aqueous media depending on the balance between two opposite interactions- electrostatic repulsion and hydrophobic attraction. This balance can change by addition of some organic solvent to the aqueous solution, and as a result, we can control the micellar structure and micellization equilibrium by changing the solvent content. In the present study, we have investigated the micellization equilibrium of an amphiphilic statistical copolymer consisting of sodium 2-(acrylamido)-2- methylpropanesulfonate and hexyl methacrylate in mixtures of water and methanol by sedimentation equilibrium and fluorescence.

• Journal of Genetic Medicine
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• v.10 no.2
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• pp.117-119
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• 2013

Double trisomy mosaicism of two different cell lines is extremely rare, particularly those that involve constitutional trisomy 8. We report a case of 47,XXX/47,XX,+8 in a 12-year-old female presenting with several skeletal anomalies. She exhibited distinct phenotypic features such as tall stature, deviation of the left middle finger, webbing of both thumbs and flexion deformities of the both third and fifth distal intermediate phalanges. A mild impulse-control disorder was observed, without mental retardation. Chromosomal and fluorescence in situ hybridization analysis demonstrated double trisomy mosaicism both on lymphocytes and buccal epithelial cells.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2001.05a
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• pp.75-90
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• 2001

For the purpose of helping development of a GDI(Gasoline Direct Injection) engine, the in-cylinder phenomena, such as the spray behaviors and fuel distributions, unburned fuel, and flame characteristics were investigated in a single cylinder GDI engine. The GDI engine was equipped with a swirl type electronic injector and SCV(Swirl Control Valve). PLIF(Planar Laser Induced Fluorescence) system with KrF Excimer laser was used for the measurements of the fuel distributions. The effects of the injector specifications, such as the spray cone angle and the offset angle on the fuel distributions and combustion characteristics were investigated. As a result, it was found that the injected fuel spray collided with the bottom of the bowl and moved upward along the exhaust side wall of the piston bowl. This fuel vapor played a important role in the instance of spark ignition. The injector specifications has a great influence on the flame characteristics.

• The Korea Journal of Herbology
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• v.21 no.1
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• pp.63-69
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• 2006

Objectives : This study was designed to investigate the effects of Cervi Pantotrichum Cornu and Cervi Cornu on the growth of longitudinal bone in the adolescent male rats. Methods : Longitudinal bone growth was measured by fluorescence microscopy. To examine the effects on the growth plate metabolism, the heights of growth plate and the induction of local bone morphogenetic protein-2 were measured. Results : Treatment of Cervi Pantotrichum Cornu significantly enhanced longitudinal bone growth compared with control group. However, Cervi Cornu did not show the significant effects. Conclusion : Cervi Pantotrichum Cornu enhanced longitudinal bone growth and promoted the induction of local bone morphogenetic protein-2 of growth plate in adolescence male rats.

• Journal of the Korea Safety Management & Science
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• v.19 no.1
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• pp.115-122
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• 2017

This study analyzed that the standard of school supplies affects the satisfaction of certification. Safety requirements of school supplies in the Republic of Korea is enforced by court duty certification. Therefore, a test was conducted to compare the safety requirements of the United States and Europe and the survey was conducted to know how much manufacturers and importers are satisfied with the certification. To prepare management measures for harmful substances contained in children's product, XRF(X-ray Fluorescence Spectrometry) is utilized to analyze lead(Pb) and cadmium(Cd) in PA(paint), SP(sheet) and PVC(Poly Vinyl Chloride). The results of comparative analysis of ICP(inductively coupled plasma) is analyzed statistically with measured values.

• Research in Plant Disease
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• v.24 no.3
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• pp.242-247
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• 2018

Pseudomonas chlororaphis O6 induces systemic tolerance in plants against drought stress. A volatile, 2R, 3R-butanediol, produced by the bacterium causes partial stomatal closure, thus, limiting water loss from the plant. In this study, we report that applications of extracellular polymeric substances (EPS) from P. chlororaphis O6 to epidermal peels of leaves of Arabidopsis thaliana also reduce the size of stomatal openings. Growth of A. thaliana seedlings with applications of the EPS from P. chlororaphis O6 reduced the extent of wilting when water was withheld from the plants. Fluorescence measurements showed photosystem II was protected in the A. thaliana leaves in the water stressed EPS-exposed plants. These findings indicate that P. chlororaphis O6 has redundancy in traits associated with induction of mechanisms to limit water stress in plants.