• 한국약용작물학회지
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• 제26권2호
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• pp.119-126
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• 2018

Background: The flowering plant Hippophae rhamnoides L. has been used for many studies on fruit or leaf extracts. This study was conducted to investigate the development of a new cosmetic material from H. rhamnoides fruits and leaves that have by antioxidant, anti-inflammatory and wrinkle improvement activities. Methods and Results: The antioxidant abilities of H. rhamnoides extracts, including of a water-soluble fruit powder (FW), a fatsoluble fruit powder (FF), a supercritical extract of fruit by-product (BS), and a mixture of leaf and fruit (MIX), were investigated in vitro. A DPPH radical assay for antioxidant activity was performed for these fractions alongside assay to evaluate the total phenolic and flavonoid content (TPC and TFC). As expected, the MIX had the highest DPPH radical scavenging activity ($RC_{50}=10.27{\mu}g/m{\ell}$), and the TPC and TFC also were highest in MIX ($225.7mg{\cdot}GAE/g$, and $25.18mg{\cdot}QE/g$, respectively). Nitric oxide (NO) production in LPS-induced RAW264.7 cells was estimated and the results indicated an over 75% decrease of NO production in FF and MIX. In other assays, the highest elastase inhibitory activity was found in FW. Conclusions: These results revealed that H. rhamnoides extracts have a high potential for antioxidant, anti-inflammatory and antiwrinkle activities. H. rhamnoides products are suggested to be applied as the functional materials of cosmetic ingredients.

• Journal of Forest and Environmental Science
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• 제31권3호
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• pp.214-224
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• 2015

The economy of India and so also of many Asian countries depends on bamboos and their uses are not only in domestic items but also in rural housing and raw materials to several industries and germplasm characterization is an important link between the conservation and utilization of plant genetic resources. Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering coupled with different biotic agencies and environmental factors. Identification and genetic relationships among accessions of Thamnocalamus falconeri were investigated using morphology and random amplified polymorphic DNAs (RAPD) technique. Analysis started by using 51 vegetative characters and forty two 10-mer primers that allowed us to distinguish different genotypes hailing from different eco- zones of Garhwal Himalayas (India). The selected primers (12) were used for identification and for establishing a profiling system to estimate genetic diversity. A total of 79.33% polymorphism was estimated by using 12 selected primers. The genetic similar analysis was conducted based on binary digits i.e. presence (1) or absence (0) of bands, which revealed a wide range of variability among the species whereas genetic relatedness was quite high based on vegetative characters. Cluster analysis clearly showed two major clusters for both of the markers viz. morphology and RAPD belonging to 10 accessions of T. falconeri. Two major clusters were further divided into minor clusters. Cluster based on RAPD marker showed grouping of accessions of closed locality whereas analogy was reported for vegetative traits. The RAPD technique has the potential for use in species identification and genetic relationships studies of bamboo for breeding program.

• Preventive Nutrition and Food Science
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• 제11권4호
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• pp.311-317
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• 2006

Phenolic compounds in safflower seeds were recently found to stimulate bone formation and increase plasma HDL cholesterol levels in estrogen deficient rats, and to inhibit melanin synthesis. Nine phenolic compounds: $N-feruloylserotonin-5-O-{\beta}-D-glucoside,\;8'-hydroxyarctigenin-4'-O-{\beta}-D-glucoside,\;luteolin-7-O-{\beta}-D-glucoside$, N-(p-coumaroyl)serotonin, N-feruloylserotonin, 8'-hydroxy arctigenin (HAG), luteolin (LT), $acacetin-7-O-{\beta}-D-glucuronide$ (ATG) and acacetin (AT), were quantified by HPLC in safflower (Carthamus tinctorius L.) seeds during growth and processing. During growth, levels of the nine phenolic compounds in the seeds increased progressively with increasing growth stages, reached a maximum on July 30 (42nd day after flowering), and then remained relatively constant. During the roasting process, levels of phenolic compounds, except HAG, LT and AT, generally decreased with increased roasting temperature and time, whereas those of HAG, LT and AT increased progressively with increased roasting temperature and time. During the steaming process, levels of other phenolic compounds except HAG and AT generally tended to increase with increased steaming time, whereas those of HAG and AT were scarcely changed. During the microwave treatment, quantitative changes of phenolic compounds were similar to the roasting process, although there were some differences in levels of phenolic compounds between two heat treatments. These results suggest that the steamed safflower seeds after harvesting on late July may be useful as potential dietary supplement source of phenolic compounds for prevention of several pathological disorders, such as atherosclerosis and osteoporosis and aging.

• 대한본초학회지
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• 제31권4호
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• pp.87-91
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• 2016

Objectives : In order to confirm whether extracts of different parts of Zostera marina (ZM), a marine flowering plant, can be used as cosmetic ingredients, this study evaluated their cytotoxicity and cytoprotective effects against ultraviolet B (UVB). Inflammatory responses induced by UV stimuli are also associated with the aging of the skin.Methods : We investigated the effects of ZM extracts on cells through the water soluble tetrazolium salt-1(WST-1) assay for cell viability. In order to investigate the anti-inflammatory effects, we evaluated the suppression of Cyclooxygenase-2 (COX-2) expression by ZM extracts in HaCaT cells with UVB-induced damages, and also evaluated the production of Prostaglandin E₂ (PGE₂) in RAW 264.7 cells with LPS-induced damages.Results : High cell viabilities above 90% were observed in all types of ZM extracts, except for whole ZM extract at 0.5 mg/ml; in keratinocytes with UVB-induced damages, the cell viabilities were above 80% when treated with all types of ZM extracts. We confirmed their anti-inflammatory effects by investigating the suppression of inflammatory mediators. In keratinocytes with UVB-induced damages, COX-2 expression decreased in the experimental group treated with ZM extract. Similarly, in RAW 264.7 cells where inflammation was induced with LPS, the biosynthesis of PGE₂ was inhibited.Conclusion : These results suggest that ethanol extracts from Zostera marina may have value as the potential anti-inflammatory medicinal plant. Also based on the abovementioned results, ZM extract protects skin cells from UV-induced damages, and thus can be used in topically applied products for skin protection.

• Nutritional Sciences
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• 제9권2호
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• pp.117-123
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• 2006

G. japonicum is a perennial hem and the flowering plant has been used as a diuretic and an astringent in Japan and China. However, little information is available about the anti-inflammatory action of G. japonicum. Therefore, the objective of this study was to investigate the antiinflammatory action of fractions from G. japonicum methanol extract. Inhibition of NO production was observed when cells were cotreated with fractions of G. japonicum and lipopolysaccharide. We observed that ethyl acetate fraction of G. japonicum inhibited NO production by LPS-activated RAW 264.7 cells, and that the suppression induced by ethyl acetate fraction of G. japonicum was associated with antioxidant activity and direct NO clearance. In addition, only ethyl acetate fraction of G. japonicum inhibited stimulated $PGE_2,\;TNF-\alpha,\;IL-1\beta$ production, whereas water and methyl chloride fractions showed no such effects. The ethyl acetate fraction of G. japonicum methanol extract showed a remarkable scavenging activity on the 1,1-diphenyl-2 picrylhydrazyl radical. Based on the results, ethyl acetate fraction of G. japonicum may be useful source as natural antioxidants and antiinflammation. Therefore, the results obtained from this study provide an alternative protective mechanism of ethyl acetate fraction of G. japonicum and provide information on the potential use of ethyl acetate fraction of G. japonicum in chemoprevention or pathogenic conditions related to overproduction of NO and $PGE_2$. However, the mechanism of the inflammatory effect must be evaluated through various parameters for induction of NO production.

• 생태와환경
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• 제46권3호
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• pp.319-331
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• 2013

Recent climate change, which is mostly ascribed to anthropogenic activities, is believed to be a major factor leading to biodiversity decreases and ecosystem service deteriorations. I have reviewed recent studies on climate change effects for many ecological processes involved with plants, in order to improve our understanding of the nature of ecological complexity. Plants in general have better growth and productivity under high levels of $CO_2$, although the long term effects of such $CO_2$ fertilizers are still controversial. Over the last 30 years, the Earth has been greening, particularly at higher latitudes of the Northern Hemisphere, perhaps due to a relaxation of climatic constraints. Human appropriation of net primary productivity (NPP), which corresponds up to 1/3 of global NPP, is ultimately responsible for climate change and biodiversity decreases. Climate change causes phenological variations in plants, especially in regards to spring flowering and fall leaf coloring. Many plants migrate polewards and towards higher altitudes to seek more appropriate climates. On the other hand, tree mortality and population declines have recently been reported in many continents. Landscape disturbance not only hinders the plant migration, but also makes it difficult to predict the plants' potential habitats. Plant and animal population declines, as well as local extinctions, are largely due to the disruption of species interactions through temporal mismatching. Temperature and $CO_2$ increase rates in Korea are higher than global means. The degree of landscape disturbances is also relatively high. Furthermore, long-term data on individual species responses and species interactions are lacking or quite limited in Korea. This review emphasizes the complex nature of species responses to climate change at both global and local scales. In order to keep pace with the direction and speed of climate change, it is urgently necessary to observe and analyze the patterns of phenology, migration, and trophic interactions of plants and animals in Korea's landscape.

• 한국자원식물학회지
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• 제8권3호
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• pp.265-274
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• 1995

유자(Citrus junos)에 있어서 수관내 위치별 엽, 엽면적, 엽중의 특성차이와 정아의 발생상태, 화아분화와 과실 품질에 미치는 기초자료들을 얻고져 실험을 실시하였던 바 결과는 다음과 같다. 1. 동, 서, 남, 북의 방향별 엽수, 엽면적, 엽중에 있어서는 유의차가 없었다. 2. 수관의 상하간에는 엽수와 엽면적은 유의차가 었었다. 3. 정아의 횡경과 종경은 방향별로는 차이가 없었지만, 상하간에는 유의한 차이를 나타내었다. 4. 투과광도와 엽 및 엽면적과의 상관관계는 광도 감소율에 따라 엽의 엽비중이 가장 큰 영향을 받았으며, 단과지당 전체엽중, 평균 엽중, 단과지 정아의 횡경, 종경도 높은 상과관계를 나타내었다. 5. 화아분화에 차광은 비효과적이었으며, GA처리도 같은 경향을 나타내었다. 6. 적과는 1과당엽수를 40잎 이상으로 하는 것이 화아분화율과 과실의 품질을 향상시킬 수 있었다.

• 한국자원식물학회지
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• 제27권6호
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• pp.701-706
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• 2014

Three highbush blueberries (Vaccinium corymbosum cvs. Bluecrop, Coville, and Northland) grown in an open field and in a rain shelter were compared to evaluate the potential benefits of rain-sheltering systems on growth and fruit quality. Shrubs grown in rain shelter flowered 5-14 days earlier and were in full bloom earlier than those in the open field. All three cultivars set fruit by nearly 90% of flowers when they were grown in the open field, but was markedly reduced when they were grown in the rain shelter, to approximately 50% for 'Bluecrop' and 'Coville'. Fruit sets ratio of 'Northland' grown in the in the rain shelter was 14.5. Fruit from greenhouse-grown 'Northland' were larger, heavier, and had more seeds per berry. Soluble sugar content (SSC) of fruits varied both with the cultivars and growing conditions. SSC of fruits grown in the greenhouse was higher in 'Bluecrop' compared to that grown in the field, but this pattern was reversed in 'Northland'. Titratable acid was significantly higher in fruits from rain shelter-grown shrubs of both 'Bluecrop' and 'Northland' than in fruits from open field-grown shrubs (P < 0.05), although there was no difference in TA with respect to growing conditions for 'Coville'. The number of seed per fruit, fruit length and diameter, weight, SSC, and TA were similar in 'Coville' shrubs grown in the open field and the rain shelter. Fruit firmness of 'Bluecrop', 'Coville', and 'Northland' was higher in the rain shelter than in the open field. 'Coville' and 'Northland' fruits harvested in the open field had higher blue chroma than those harvested in the rain shelter. However, this pattern was reversed for 'Bluecrop'. On the whole, sheltering from rain affected most fruit characteristics of the three cultivars differently. Therefore, suitable blueberry cultivars for the rain shelter should be chosen by purpose.

• Molecules and Cells
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• 제40권8호
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• pp.587-597
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• 2017

MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. We employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissuespecific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5' rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in H. syriacus. Collectively, this study provides the first reliable draft of the H. syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in H. syriacus.

• 한국육종학회지
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• 제41권1호
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• pp.61-67
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• 2009

"Early Valley", is an early maturing potato cultivar with high yield potential. "Early Valley" is a clonal selection resulting from the cross between 'Suncrisp' and 'A87109-10'. It has medium plant height and light green foliage. "Early Valley" has medium flowering habit and white flowers. Tubers are smooth, yellow skin, light yellow flesh, round tuber shape, medium eye depth, and medium dormancy and good keeping quality. It has stable yield under wide range of climatic conditions. "Early Valley" is resistance to late blight, but moderately susceptible to common scab and hollow heart. This cultivar is also resistant to potato rotting at harvesting during the raining season. "Early Valley" has high level of antioxidant activity (about three times higher) and vitamin C (higher by 40%) than the 'Superior'. This cultivar has high level of tuber uniformity and capable of yielding 36.56 t/ha which is 17.07% higher than the control potato cultivar 'Superior' under optimum agronomical practices.