• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.83-89
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• 2018

The interplay of plant hormones is one of the essential mechanisms for plant growth and development. A recent study reported that Brassinosteroids (BR) and ABSCISIC ACID (ABA) interact antagonistically in early seedling developments through the BR-mediated epigenetic repression of ABSCISIC ACID-INSENSITIVE 3 (ABI3). However, the other physiological roles of the BR-mediated regulation of ABI3 and ABA responses beyond early seedling developments remain largely unknown. Here, we showed that the activation of BR signaling by high temperatures promotes flowering time through the suppression of ABI3 expressions. Elevated ambient temperature induced early flowering in wild type Col-0 plants, but not in BR-defective bri1-116 mutant plants. Conversely, a hyper BR biosynthetic dwf4-D mutant displayed more sensitive thermomorphic long shoot elongation and early flowering. Both expression patterns and physiological responses supported the biological roles of ABI3 in the regulation of floral transition and reproduction under high temperature conditions. Finally, we confirmed that the lowered expressions of the transcript and protein levels of ABI3 brought on by elevated temperature were correlated with warmth-induced early flowering phenotypes. In conclusion, our data suggest that the BR- and warmth-mediated regulation of ABI3 are important in thermomorphic reproductive phase transitions in plants.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.213-237
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• 1987

In vitro flowering system may minimize the confounded influence of non-floral meristem parts of plants in studying the relationship of a given treatment and flowering responses. We have induced flower buds from plantlets regenerated from zygotic embryo-derived somatic embryos of ginseng, which circumvented the normal 2-year juvenile period before flowering. The result suggests that the adulthood of ginseng root explants in the experiment previously conducted by Chang and Hsing (1980; Nature 284: 341-342) is not prerequired to flowering of plantlets regenerated through somatic embryogenesis. We have also induced flower buds from elongated axillary brandches from cotyledonary nodes by culturing ginseng zygotic embryos, seedlings, and excised cotyledonary nodes. It was found that 6-benzyladenine (BA) supplemented to the medium was essential for flowering, whereas abscisic acid (ABA) was inhibitory. Gibberellic acid(GA3) was also required for flowering when ABA was present with BA in the medium. The results suggest that cytokinins, gibberellins, and inhibitors play primary, permissive, and preventive roles, respective-ly, in the induction of flowering of ginseng. Tran Thanh Van (1980; Int. Rev. Cytol., Suppl. IIA: 175-194) has developed the "thin cell layer system" in which the induction of shoots, roots, or flower buds from epidermal layer explants were controlled by culture conditions and exogenous growth regulators in the medium, Utilizing the thin cell layer system, Meeks-Wagner et al. (1989; The Plant Cell 1: 25-35) have cloned genes specifically expressed during floral evocation. However, the system is too tedious for obtaining a sufficient amount of plant materials for biochmical and molecular biological studies of flowering. We have developed a garlic callus culture system and one obvious advantaging over the thin cell layer system is that an abundant cells committed to develope into flower buds proliferate. When the above cells were compared by two-dimensional gel electrophoresis with those which have just lost the competence for developing into flower buds, a few putative proteins specific to floral evocation were detected. The garlic callus culture system can be further explored for elucidation of the molecular biological mechanism of floral evocation and morphogenesis.hogenesis.

• Journal of People, Plants, and Environment
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• v.24 no.4
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• pp.355-363
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• 2021

Background and objective: This study was conducted on Forsythia velutina, a special plant, in Gyeongsangnam-do Arboretum under the Gyeongsangnam-do Forest Environment Research Institute, which is located in the southern part of Korea. Methods: The research aimed to analyze the flowering characteristics of the plant by calculating the optimal temperature and humidity according to the flowering time and flowering period for 8 years from 2010 to 2017 in order to provide basic data for bioclimate studies of endemic plants. Results: It was observed that the Forsythia velutina showed a life cycle from mid-March and to mid-November. Average growth period was 243 (± 6.5) days. In testing the reliability of a single variable according to the meteorological factors, the Cronbach's Alpha was 0.701, which indicates that the findings were relatively reliable. The average date of flowering was March 16 (SD = 5.8) and the average date on which blossoms fall was March 29 (SD = 5.2). A substantial difference in flowering period was observed from year to year 11 to 23 days, with an average of 16 days (± 4.7). The temperature and humidity in February to March, which affect the flowering, were 2.9-5.5℃, and 66.5-73.0%, respectively, and showed differences every year. Conclusion: The correlation between flowering time and meteorological factors was positive, and the highest daily temperature and average daily temperature had the highest significance. When establishing basic data on plant species for the conservation of endemic plants, the changes in life cycle events and weather conditions are identified. It is believed that it will be helpful in establishing a conservation strategy for the plant species in the future.

• The Korean Journal of Ecology
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• v.21 no.5_1
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• pp.457.2-486
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• 1998

Ecological success of introduced species is frequently attributed to the reproductive characters of those species. We examined the relationship between both flowering season and plant size and reproductive characters in two introduced species of Taraxacum, e.g., T. officinale and T.laevigatum. Seventy six plants of T. officinale and 23 plants of T. laevigatum were randomly tagged from a population at the sungshin Women's University in April of 1997. The size and number of vegetative characters such as rosette diameter, leaf number, and the largest leaf length and width were measured for each plant at the onset of flowering. The infructescence was collected continually from each plant before seed dispersal from April to August of 1997. The number of infructescence per plant, and seed number and total seed weight per infructescence were measured. Mean individual seed weight per infructescence was also obtained. T. officinale possessed larger sized, but smaller number of, leaves than T. laevigatum, thereby resulting in no significant difference in total leaf area. There was a trend that vegetatively larger plants in both of these species produced reproductive characters larger in size or greater in number, except for seed number and total seed weight per infructescence in T. laevigatum. All reproductive characters examined in T. officinale decreased toward the end of flowering season. In T. laevigatum, infructescence number per plant also declined during a flowering season: however, mean seed number per infructescence increaed toward the end of flowering season with no significant seasonal change in the total seed weight per infructescence. T. officinale produced on average about twice as many seeds per plant as T. laevigatum during a growing season. These two Taraxacum species produced quite small seeds in size compared to the endemic species, exhibiting a far better dispersal ability of introduced species. These data demonstrate that most reproductive characters decline in size and number in both species during a long flowering period, but the two species appear to employ different strategies to achieve reproductive success in disturbed habitats.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.7-11
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• 1999

This experiment was conducted to investigate the effects of soil conditioners, such as lime, borax, poultry waste sawdust manure (P.W.S.M) and mixed oil cakes (M.O.C), on the rutin biosynthesis and the yield of buckwheat. The content of phosphorus ($P_2O_5$) in buckwheat plants before flowering was higher at before flowering than that of flowering time at all plots. The content of nitrogen (N), potassium ($K_2O$), calcium (CaO) and magnesium (MgO) was lower at before flowering than those of flowering time at all plots. The contents of rutin in buckwheat plant at flowering time was higher than that at before flowering content of rutin in leaf was higher than that in stem. The contents of rutin in buckwheat plant was the highest at the plot treated with borax (B) compared to other plots. Correlation between rutin and amino acid was positive in buckwheat leaf, while it was negative in buckwheat stem. The yield of buckwheat was 21 percent higher at the plot treated with borax than the plot applied with the other three ingredients.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.2
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• pp.130-133
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• 2001

The purpose of this study was to investigate the decreased ratio of growth and yield by delayed seeding and flowering because drought of spring season often cause to delay seeding and sprout emergence. Equation of linear regression, y=-11.914x＋818.61 ($R^2$=0.916) and y=-16.96lx＋913.98 ($R^2$=-0.885) were derived from relationship between sowing date and yield of leading variety, Yangbaeckkae in 1999 and 2000, respectively. Yield was decreased by 7, 24, 40, 57, 74%, respectively, according as sowing date was delayed more 5, 15, 26, 36, 46 days than May 15, standard sowing date under the culture mulched with black P.E. film. Number of capsules per plant and length of stem bearing capsule were greatly decreased, while plant height, stem diameter, and day to flowering were affected little by delayed seeding date. Equation of linear regression, y=-0.7081x＋41.04 ($R^2$=0.861) was derived from relationship between flowering date and yield of 33 accessions. Yield was decreased by 7.7, 8.3, 9.2, 10.1, 11.2%, respectively, according as flowering date was delayed more 1, 2, 3, 4, 5 days than July 3, normal flowering date of Yangbaeckkae when it was sown on May 15, and the more flowering date was delayed, the more yield was decreased. Number of capsules per plant and length of stem bearing capsules were greatly decreased, but plant height and harvest index were decreased little by delay of flowering date

• Journal of Ecology and Environment
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• v.47 no.3
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• pp.134-145
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• 2023

Background: The flowering and fruiting periods play an important role in biological processes. The deciduous dipterocarp forest is an important forest type in Thailand, however the phenological studies are still limited, particularly in different plant life forms. Thus, the present study focused on the flowering and fruiting phenology of herbs, climbers, shrubs, and trees in the deciduous dipterocarp forest at Lampang province of Northern Thailand. Field visits were made to record plant life forms and observe reproductive phenological events at monthly intervals from November 2018 to October 2019 and September to December 2020. Results: The phenological observations were based on 126 species of 45 families and 102 genera. Flowering and fruiting periods showed similar patterns in herbaceous plants, climbers, and shrubs. Most of these species produced flowers and fruits from the end of the rainy season (October) to the winter season (November-January). Whereas most of flowering and fruiting trees were found from the summer season (March-April) to the beginning of the rainy season (May-June). Most of the dry-fruited species occurred during the dry period (winter and summer seasons), while the majority of fleshy-fruited species dominated in the wet period (rainy season). The statistical analysis supported the phenological patterns of flowering and fruiting in the present study. There were significant negative correlations between the number of flowering and fruiting species and temperature. The number of flowering and fruiting species is significantly impacted by the interaction between seasons and plant life forms. Conclusions: Plant life form seems to be the important factor that affects the different phenological patterns in the studied plants. The abiotic and biotic factors play major roles in reproductive phenology. However, long-term study and in-depth phenological observations are necessary for better understanding.

• Journal of Plant Biology
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• v.19 no.1
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• pp.14-18
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• 1976

Inhibition of flowering in Lemna perpusilla 6746 by 30 mM sucrose was reversed by the addition of acetylcholine (>$10^{-4}M) supplemented with 10^{-4}M$ ascorbic acid to 1/10-strength Hunter's growth medium. The reversible effect of acetylcholine was found to be greater at early stages of flowering than in the later period. Promotive effects of both acetylcholine ($10^{-3}M) and eserine(10^{-5}M$) on flowering in the short-day plant under various photoperiodic conditions were studied. It was indicated that the application decreased length of the critical dark period for the floral induction, and it was also shown that the endogenous status of acetylcholine was involved in the floral response which had a correlation with phytochrome. Interruption of inductive dark periods by red irradiation (1min) immediately followed by far-red light (1 min) completely inhibited flowering, while the addition of acetylcholine and eserine to the medium under the same condition slightly promoted flowering, indicating possible involvement of phytochrome system in acetylcholine activity for photoperiodic sensitivity of floral response in Lemna perpusilla 6746.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.574-578
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• 2001

The effects of seed soaking (8 and 24 hours) and foliar spray of plant growth retardants (daminozide, chlormequat and uniconazole) on growth control in zinnia were investigated. For seed soaking of 'Dream Land Rose', the changes in plant height was not affected by seed soaking time (8 and 24 hours), while it was severely suppressed by high concentration of chemicals tested. An application of $125mg{\cdot}L^{-1}$ uniconazole was most effective for plant height suppression. Flowering was promoted by all treatments of chemicals. However, for 'Dream Land Scarlet', plant height control at 24 hours seed soaking showed more effective than 8 hours, and all treatments of chemicals decreased plant height. Uniconazole gave the best result for plant height control. Flowering tended to be hastened by the treatment of chemicals. For foliar spray, plant height was remarkably decreased at high concentration of uniconazole. Flowering was promoted by chlormequat and uniconazole in both 'Dream Land Rose' and 'Dream Land Scarlet'. Also, daminozide decreased number of petals.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.236-242
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• 2012

We investigated the influences of night interruption (NI) and night temperature on flowering and flower coloration in Cymbidium. Cymbidium 'Red Fire' and 'Yokihi' were grown under a 9 hours photoperiod (control), a 9 hours photoperiod with NI at a low light intensity (LNI) of 3-7 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, or a 9 hours photoperiod with NI at a high light intensity (HNI) of 120 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for four hours (22:00-02:00 HR) for 16 weeks during the reproductive growth stage (Experiment 1). Thirty month-old Cymbidium 'Red Fire' plants with initiated flowering buds were placed in four different growth chamber with night temperature set points of 6, 9, 12, or $15^{\circ}C$ for 16 hours (18:00 to 09:00 HR) and a daytime temperature of $25^{\circ}C$ (Experiment 2). In Experiment 1, the numbers of visible buds and flowers increased, and time to flowering decreased in both the LNI and HNI treatments, as compared to the control in both cultivars. Red color in Cymbidium 'Red Fire' increased by both LNI and HNI, as evidenced by an increased $a^*$ in plants grown under these conditions, relative to those grown under the control condition. Number of days to visible buds at 9-$15^{\circ}C$ ranged from 31-34 days, as compared to 39 days at $6^{\circ}C$ in Experiment 2. Although as the temperature increased days to flowering decreased when the plant was grown at $15^{\circ}C$ as compared to 6, 9, or $12^{\circ}C$, the red color ($a^*$) also decreased. The number of flowers and percent flowering increased when the night temperature was maintained higher than $9^{\circ}C$. Therefore, NI treatment and maintaining the night temperature at approximately 9-$12^{\circ}C$ during the winter season after flower spike initiation in the reproductive developmental growth stage improve flower quality and controls flowering time.