• The Korean Journal of Pain
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• v.7 no.2
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• pp.162-169
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• 1994

Blockade of cervicothoracic sympathetic ganglion (stellate ganglion controls pain on face, head, neck, shoulder, upper limbs, and upper chest, including their viscera and sympathetically maintained pain. This procedure also increases blood flow to the above areas and relieves hyperreactivity of sympathetic nervous system. Clinically, repeated stellate ganglion blocks with local anesthetic agent may become difficult with complications such as accidental intravascular or subdural injection, recurrent laryngeal nerve or bracheal plexus paralysis, pneumothorax and edema on injection site. Therefore, at times long-term cervicothoracic ganglion block with neurolytics is necessitated but its applications are prohibited by the critical structures surrounding ganglion. There are also few reports of neurolytic stellate ganglion block. This study was performed to observe the complications, gross changes of surrounding structures, and microscopic findings of ganglion cells after neurolytic block and to certify the possibility of clinical use of neruolytic stellate ganglion block. The unilateral superior cervical sympathetic ganglion of rabbit was blocked with absolute ethyl alcohol 0.4 ml at the level of cricoid cartilage. Normal ganglion was used as a control and 5 animals were sacrificed at each intervals of 7, 15 and 50 days after block. The results were as follows; 1) All experimental animals showed no specific changes of behavior, motor function. No necrotic tissues were present in the block area during the observation period. There were some gross scar tissues along the fascia of muscles surrounding the needle injection site, but gross atrophy of muscles or injured major vessels were not found. 2) Microscopically, structures of normal ganglion of rabbit were very similar to those of humans. Seven days after absolute ethyl achohol injection there were marked edema of ganglion cells and nuclei with irregular nuclear membrane. Some of the ganglion cells lost their nuclei and showed degenerative changes. Fifteen days after block, cell edema were decreased and loss of the Nissl's body was prominant. The ganglion cell structures looked close to normal but the cytoplasm and nucleus were generally contracted 50 days after block. These results suggest absolute ethyl alcohol injection on cervical sympathetic ganglion with above method mainly blocks pre- and post-synaptic fibers and the long-term neurolytic blockade of this ganglion may be possible in rabbits.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.19 no.3
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• pp.194-204
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• 2007

A numerical analysis and hydraulic experiments were undertaken to investigate the head loss occurring when a flow passes through vertical perforated walls. The numerical analysis applied continuity, momentum and energy equations to the control volumes that were set near the perforated wall. Non-dimensional equations were then derived to calculate both upstream depth and head loss for the given values of downstream depth and velocity. The hydraulic experiments were performed with several single and triple perforated plates varying their opening ratios and intervals. The numerical results with the single plates were compared with the experimental results, and it was shown that the contraction coefficient of the vertical line jet formed after the perforated plates relies on downstream Froude number as well as opening ratio. Based on the experimental results, empirical formulas were formulated. Finally, the formulas were applied to the triple plates sequentially from downstream side to upstream side, and it was found that in general the predicted values nicely agreed with the experimental results.

• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.1971-1982
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• 2017

Piliated Lactobacillus rhamnosus (pLR) strains possess higher adherent capacity than non-piliated strains. The objective of this study was to isolate and characterize probiotic pLR strains in human fecal samples. To this end, mouse polyclonal antiserum (anti-SpaA) against the recombinant pilus protein (SpaA) of L. rhamnosus strain GG (LGG) was prepared and tested for its reactivity and specificity. With the anti-SpaA, a method combining the de Man, Rogosa, and Sharpe (MRS) agar plating separation and colony immunoblotting (CIB) was developed to isolate pLR from 124 human fecal samples. The genetic and phenotypic characteristics of the resultant pLR isolates were compared by randomly amplified polymorphic DNA (RAPD) fingerprinting, and examination of adhesion to Caco-2 cells, hydrophobicity, autoaggregation, and in vitro gastrointestinal tolerance. Anti-SpaA specifically reacted with three pLR strains of 25 test strains, as assessed by western blotting, immunofluorescence flow cytometry, and immunoelectron microscopy (IEM) assays. The optimized MRS agar separation plus anti-SpaA-based CIB procedure could quantitatively detect $2.5{\times}10^3CFU/ml$ of pLR colonies spiked in $10^6CFU/ml$ of background bacteria. Eight pLR strains were identified in 124 human fecal samples, and were confirmed by 16S RNA gene sequencing and IEM identification. RAPD fingerprinting of the pLR strains revealed seven different patterns, of which only two isolates from infants showed the same RAPD profiles with LGG. Strain PLR06 was obtained with high adhesion and autoaggregation activities, hydrophobicity, and gastrointestinal tolerance. Anti-SpaA-based CIB is a rapid and inexpensive method for the preliminary screening of novel adherent L. rhamnosus strains for commercial purposes.

• Analytical Science and Technology
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• v.25 no.4
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• pp.250-256
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• 2012

Two marker compounds of Achyranthis Radix, ecdysterone and inokosterone, were analyzed by HPLC on an ODS column ($250{\times}4.6$ mm, 5 ${\mu}m$) with a mobile phase of 15% acetonitrile containing 0.08% formic acid at a flow rate of 1.0 mL/min and a detection wavelength of UV 254 nm. The method was validated by ICH guideline and applied to the monitoring of marker compounds in 93 samples of Achyranthis Radix collected at various areas in Korea and China. The new content criteria of ecdysterone and inokosterone, established using linear regression method were 0.033% and 0.020%, respectively. When the new content criteria were applied to the quality control test of commercial Achyranthis Radix, 95.4% of total samples including 100% of Korean and 92.6% of Chinese samples were passed the test. Application of new content criteria could protect the Korean products and decrease the distribution of Chinese products with lower quality.

• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.1
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• pp.77-82
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• 2000

Seven constructed and three natural tidal flats were compared to evaluate state-of- the-art of creation and restoration technology for tidal flats. parameters studied were physico-chemical and biological characteristics of soils and rate of respiration. The natural tidal flats had higher contents of silts, nitrogen and organic matter compared to the constructed ones. The natural ones had reductive Bone below 2 cm whereas the constructed ones had oxidative zone from the surface to below 20 cm. The bacterial population in the soil of the constructed tidal flats was one to two magnitudes lower than that in the natural ones. Biomass of macrobenthos and microbial respiration rate, however, were not different significantly between the natural and the constructed tidal flats. The purification capacity by diatom+bacterial+meiobenthos and macrobenthos in the constructed tidal flats was higher than that in the natural ones due to deeper permeable layer for purification in the constructed tidal flats. There was an exceptional constructed tidal flat with similar physico-chemical and biological characteristics to natural ones. Shearing stress to the surface of the tidal flat by the flow of seawater was as low as that of natural ones. These hydraulic conditions seemed to be a controlling factor on structures and functions of tidal flats. The control of hydraulic condition seemed to be one of the most important factors to create natural-like tidal flats.

• Fisheries and Aquatic Sciences
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• v.13 no.4
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• pp.294-299
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• 2010

The effects of dietary nutrient composition on compensatory growth of grower olive flounder (Paralichthys olivaceus) under different feeding regimes at suboptimal temperature were determined. Four hundred five fish weighing 271.2 g were distributed into 27 300 L flow-through tanks (15 fish per tank). Nine treatments were prepared in triplicate: fish were hand-fed with control (C) diet for 12 weeks (12WF-C); four groups of fish were starved for 1 week and then fed C, high-protein (HP), high-lipid (HL), or combined high-protein and high-lipid (HPL) diets for 11 weeks; these groups are referred to as 11WF-C, 11WF-HP, 11WF-HL, and 11WF-HPL, respectively. Four other groups of fish were starved for 2 weeks and then fed C, HP, HL, and HPL diets for 10 weeks; these groups are referred to as 10WF-C, 10WF-HP, 10WF-HL, and 10WF-HPL, respectively. Weight gain and specific growth rate of fish from 12WF-C group were greater than those of fish from 11WF-C, 11WF-HP, 11WF-HL, 10WF-C, 10WFHP, and 10WF-HL groups, but not different from those of fish from 11WF-HPL and 10WF-HPL groups. Feed-efficiency ratio of fish from 12WF-C, 11WF-HP, 11WF-HPL, 10WF-HL, and 10WF-HPL groups was higher than that of fish in 11WF-C, 11WF-HL, 10WF-C, and 10WF-HP groups. The results of this study demonstrated that grower olive flounder subjected to 1- or 2-week feed deprivation were able to achieve full compensatory growth at suboptimal temperature only when fed HPL diet.

• Tunnel and Underground Space
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• v.25 no.2
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• pp.155-167
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• 2015

The thermal-hydrological-mechanical (T-H-M) behavior of rock mass surrounding a high-temperature cavern thermal energy storage (CTES) operated for a period of 30 years has been investigated by TOUGH2-FLAC3D simulator. As a fundamental study for the development of prediction and control technologies for the environmental change and rock mass behavior associated with CTES, the key concerns were focused on the hydrological-thermal multiphase flow and the consequential mechanical behavior of the surrounding rock mass, where the insulator performance was not taken into account. In the present study, we considered a large-scale cylindrical cavern at shallow depth storing thermal energy of $350^{\circ}C$. The numerical results showed that the dominant heat transfer mechanism was the conduction in rock mass, and the mechanical behavior of rock mass was influenced by thermal factor (heat) more than hydrological factor (pressure). The effective stress redistribution, displacement and surface uplift caused by heating of rock and boiling of ground-water were discussed, and the potential of shear failure was quantitatively examined. Thermal expansion of rock mass led to the ground-surface uplift on the order of a few centimeters and the development of tensile stress above the storage cavern, increasing the potential of shear failure.

• Journal of Energy Engineering
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• v.24 no.2
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• pp.120-128
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• 2015

This study reports the combustion characteristics, such as burner temperature and the concentration of exhausted gas ($O_2$, $CO_x$, $NO_x$) due to the different types and pitches of the fuel supply feeder of the wood pellet boiler. The 1st grade wood pellets composed of mainly larch have been used for the experiment. In case of using the spring feeder, mean temperature of burner was approximately $821.76^{\circ}C$, and the mean concentration of oxygen, carbon monoxide, carbon dioxide and nitrogen oxide were approximately 8.88%, 93.35ppm, 12.15% and 139.83 ppm, respectively. The test result with the spring feeder was shown to approach the condition of complete combustion compared to that of a screw feeder and were in good agreement with authentication judgement standard. Furthermore, the combustion efficiency was improved according to the growth of screw pitch. The control of air flow rate from the blower and ventilator is needed to achieve the complete combustion.

• Journal of Environmental Impact Assessment
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• v.27 no.1
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• pp.73-82
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• 2018

The purpose of this study is the evaluation of the impact of increase in impervious areas due to urbanization on the pollutant discharge using the HSPF model at Musim watershed. Model calibration and validation were performed based on the observed data 2015 and 2014, all simulation items have been successfully simulated such as flow, BOD, and TP. The land cover map used in the model reflected on the land use status of the Musim watershed in 2015 and the application of the development areas and locations. As a result of simulation, during rainfall daily pollutant load with the increased impervious land increased more than that before the development. However, the pollutant load decreased during the non-rainfall time. Annual pollutant load in rainfall time was significantly higher than that in non-rainfall time, BOD and TP increased. The simulation of non-point source pollutant load was applied under two assumptions, such as the increased area of impervious land and the non-change number of point source load before and after development. As the result of a simulation, the non-point source pollutant load after development was bigger than those before development. It was necessary to take measures to control non-point source pollution at the consideration status of development.

• Journal of Chest Surgery
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• v.30 no.1
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• pp.1-10
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• 1997

The successful cardiac transplantation depends partly on the donor heart preservation by a solution that will ensure recovery of myocardial function. The purpose of this study was to perform the evaluation of various preservation solutions and to accumulate the data on the requisites for ideal preservation solution. The experimental setup was the constant pressure Langendorffs perfusion system. Isolated rabbit hearts were perfused for 20minutes with unarm Krebs-Henseleit solution, stored for 4 hours in cold preservation solution after cardioplegia, and then were reperfused for 20minutes. The 4 experimental groups were prepared Hartmann's solution group (group 1, control), modified Euro-collins solution group(group II. MEC), modified University of Wisconsin group (group n, MUW), and CK solution(made by the author) group (group W, CK). The parameters for assessing the preservation ability were levels of enzymes in freezed myocardial tissues (lactate, creatine kinase-MB and adenosine deaminase), coronary flow. left ventricular developing pressure and dpldt. In conclusion, the ability of preservation for isolated rabbit heart was excellent in CK solution and modified University of Wisconsin solution, and poor in modified Euro-collins solution, compared with Hartmann solution. CK solution has low potassium concentrations(34.2mEq/L) and includes various substrates to be salutary on myocardial preservation. This fact may indicates the necessity of further refinements in selection or composition of electrolytes and substrates.