• Journal of Advanced Marine Engineering and Technology
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• 제35권4호
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• pp.406-413
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• 2011

최근 선박에서 배출되는 온실가스를 저감하기 위한 기술로 연료전지가 주목 받고 있다. 본 연구에서는 메탄을 연료로 사용한 내부개질형 500kW급 고체산화물 연료전지의 선박 적용을 가정하여 연료전지 스택을 모델링하여 스택을 구성하는 셀의 수, 수소 변환율, 셀의 반응면적에 따른 출력 및 효율에 관한 특성을 평가하고, 공기와 메탄의 공급조건이 연료전지 스택의 성능에 미치는 영향 등에 관하여 검토하였다. 그 결과 셀의 수, 수소 변환율, 셀의 반응면적 및 공급 공기 유량이 증가할수록 스택의 출력 및 효율이 증가하였고, 메탄 공급 유량이 증가하면 출력은 증가하지만 효율은 감소하였다. 또한 Case 3의 경우에 전류가 976.4 A, 전압이 529.1 V에서 출력이 516.6 kW이고 이때의 연료전지 스택의 효율은 42.91%를 얻을 수있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권3호
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• pp.223-232
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• 2020

Sesamin, a lipid-soluble lignin originally isolated from sesame seeds, which induces cancer cell apoptosis and autophagy. In the present study, has been reported that sesamin induces apoptosis via several pathways in human lung cancer cells. However, whether mitophagy is involved in sesamin induced lung cancer cell apotosis remains unclear. This study, the anticancer activity of sesamin in lung cancer was studied by reactive oxygen species (ROS) and mitophagy. A549 cells were treated with sesamin, and cell viability, migration ability, and cell cycle were assessed using the CCK8 assay, scratch-wound test, and flow cytometry, respectively. ROS levels, mitochondrial membrane potential, and apoptosis were examined by flow cytometric detection of DCFH-DA fluorescence and by using JC-1 and TUNEL assays. The results indicated that sesamin treatment inhibited the cell viability and migration ability of A549 cells and induced G₀/G₁ phase arrest. Furthermore, sesamin induced an increase in ROS levels, a reduction in mitochondrial membrane potential, and apoptosis accompanied by an increase in cleaved caspase-3 and cleaved caspase-9. Additionally, sesamin triggered mitophagy and increased the expression of PINK1 and translocation of Parkin from the cytoplasm to the mitochondria. However, the antioxidant N-acetyl-L-cysteine clearly reduced the oxidative stress and mitophagy induced by sesamin. Furthermore, we found that cyclosporine A (an inhibitor of mitophagy) decreased the inhibitory effect of sesamin on A549 cell viability. Collectively, our data indicate that sesamin exerts lethal effects on lung cancer cells through the induction of ROS-mediated mitophagy and mitochondrial apoptosis.

• Archives of Pharmacal Research
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• 제20권4호
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• pp.297-305
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• 1997

A new serum-free defined medium was developed that supports the growth of normal rat mammary epithelial cells. Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies developed within a week and remained viable for months in culture. Upon subculture of one-week-old primary colonies, almost the same morphology of colonies was developed. The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. However, colonies cultured in BSFM supplemented with prolactin, $E_2$, and progesterone (complete hormone serum-free medium, CHSFM) showed cell-cell communication at 14 days of primary culture or of subculture. By flow cytometry with FITCPNA and PE-anti-Thy-1.1 monoclonal antibody, we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both reagents and cells positive to both reagents. It is likely that combined prolactin, cortisol, and insulin in CHSFM stimulate terminal differentiation of clonogenic cells.

• Biomolecules & Therapeutics
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• 제26권3호
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• pp.328-334
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• 2018

Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anticancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an $IC_{50}$ of $15{\mu}M$ and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of $p21^{WAF1/Cip1}$ and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5513-5518
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• 2013

IFN-${\gamma}$ plays an indirect anti-cancer role through the immune system but may have direct negative effects on cancer cells. It regulates the viability of gastric cancer cells, so we examined whether it affects their proliferation and how that might be brought about. We exposed AGS, HGC-27 and GES-1 gastric cancer cell lines to IFN-${\gamma}$ and found significantly reduced colony formation ability. Flow cytometry revealed no effect of IFN-${\gamma}$ on apoptosis of cell lines and no effect on cell aging as assessed by ${\beta}$-gal staining. Microarray assay revealed that IFN-${\gamma}$ changed the mRNA expression of genes related to the cell cycle and cell proliferation and migration, as well as chemokines and chemokine receptors, and immunity-related genes. Finally, flow cytometry revealed that IFN-${\gamma}$ arrested the cells in the G1/S phase. IFN-${\gamma}$ may slow proliferation of some gastric cancer cells by affecting the cell cycle to play a negative role in the development of gastric cancer.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제27권3호
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• pp.209-213
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• 2001

Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on anti-oral cancer activities of momordin, which was medicinal plant extracts that was revealed anticancer activities, on KB cell(oral cancer cell). The results were as follow : 1. Momordin showed the excellent anti-oral cancer activity against KB cells. Obtained IC50 value of Momordin was $10.4{\mu}g/ml$. 2. When KB cells were treated with Momordin, dose and time dependent DNA fragmentation of KB cells were observed. DNA fragmentation was initiated on three days at the concentration of $20{\mu}g/ml$ Momordin. 3. Flow cytometry showed dose-dependent apoptotic cell increase of KB cells on Momordin. 18.55% apoptotic cell were observed up to 72 hours at the concentration of $20{\mu}g/ml$ of Momordin. 4. Momordin induced nonspecific apoptosis without specific cell cycle arrest. 5. Through MTT assay, DNA fragmentation assay and flow cytometric analysis. anticancer effect of Momordin against KB cell was induce of apoptotic cell death.

• 전력전자학회논문지
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• 제13권6호
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• pp.469-475
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• 2008

본 논문에서는 소형 PEM(Proton Exchange Membrane) 연료전지 스택의 마이크로프로세서를 이용한 제어에 관하여 소개한다. 연료전지 제어의 핵심 기술인 스택 내부의 수분 관리는 부하에 의해 요구되는 연료와 온도에 따른 냉각용 공기의 유량을 적절하게 조절하고, 잉여수분을 스택으로부터 배출시킴으로써 성취된다. 이러한 주변장치(BOS: Balance of Stack)의 제어는 시스템의 안정적인 운전을 좌우하는 중요한 요소이기 때문에 이를 정확하게 제어하는 것이 매우 중요하다. 본 연구에서는 최적 운전조건에서 공기유량과 퍼지주기를 측정하고 이를 바탕으로 BOS를 제어함으로써 BOS의 소비전력을 최소화하여 연료 효율을 향상시킬 수 있었으며, 이를 실험을 통해 검증하였다. 마이크로프로세서를 이용하여 개발된 제어기는 시스템의 운전 안정성을 향상시켜 소형 연료전지 스택의 제어에 널리 사용될 것으로 기대된다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1991년도 추계학술대회 논문집
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• pp.160-164
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• 1991

The effect of various parameters, such as temperature, current density and operating valtage on the performance of phosphoric acid fuel cell stack was studied by using numerical analysis. The utilization ratio of reaction gas, inlet condition of reaction air and cooling air, inlet condition of cooling air flow latin were changed regularly, The results showed good agreements with the existing results and experimental ones.

• 신재생에너지
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• 제2권4호
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• pp.78-85
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• 2006

Selection of flow channel in the separation plate of PEMFC is very important parameter to improve its performance and reduce parasite loss. Flow patterns in the channel have great influence on the transport of hydrogen and air and removal of water generated from electrochemical reaction in diffusion layer. In this study. fluid flow in flow channel with parallel and interdigitated patterns are simulated three dimensionally on full flow domain including anode and cathode channel together. The numerical results show that the fuel cell with interdigitated flow channel represents better performance than that with parallel flow channel due to its strong convective transport across the gas diffusion layer. But the pressure drop in parallel flow channel is much more than that in interdigitated flow channel. And effects of temperature and stoichiometric number on performance can be calculated and analyzed as well. Nomenclature.

• 설비공학논문집
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• 제18권10호
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• pp.819-825
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• 2006

In a SOFC/GT (solid oxide fuel cell/gas turbine) hybrid power generation system, the recuperator is an indispensible component to enhance system performance. Since the expansion ratio to the recuperator core is very large, generally, the effective header design to distribute the flow uniformly before entering the core is crucial to guarantee the required performance. In the present study, we focus on the design of a diffuser type recuperator header with a 90 degree turn inlet port. To reduce the flow separation and recirculation flows, multiple horizontal vanes are used. The number of horizontal vanes is varied from 0 to 24. The air flow velocity is measured at 40 points just behind the core outlet by using a hot wire anemometer. Then, the flow non-uniformity is evaluated from the measured flow velocity. The experimental results showed that inlet air velocity did not effect on relative flow non-uniformity. According to increasing the number of horizontal vanes, flow non-uniformity reduced about $40{\sim}50%$ than without using horizontal vanes.