• BMB Reports
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• v.31 no.3
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• pp.296-302
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• 1998

A potentiometric flow injection biosensor for the analysis of anti-cholinesterases (anti-ChEs), based on inhibition of enzyme activity, was developed. The sensor system consists of a reactor with acetylcholinesterase (AChE) immobilized on controlled pore glass and a detector with an $H^{+}-selective$ PVC-based membrane electrode. The principle of the analysis is based on the fact that the degree of inhibition of AChE by an anti-ChE is dependent on the concentration of the anti-ChE in contact with AChE. The sensor system was optimized by changing systematically the operating parameters of the sensor to evaluate the effect of the changes on sensor response to ACh. The optimized biosensor was applied to the analysis of paraoxon, an organophosphorus pesticide. Treatment of the inhibited enzyme with pyridine-2-aldoxime fully restored the enzyme activity allowing repeated use of the sensor.

• Bulletin of the Korean Chemical Society
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• v.18 no.9
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• pp.952-957
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• 1997

Modified polymeric composite electrodes having highly dispersed CuO particles through the electrode matrix were prepared for LCEC or flow injection analysis of carbohydrates. The composite electrodes were prepared by incorporating carbon black and highly dispersed copper oxide particles in polystyrene matrix cross-linked with divinylbenzene. The analytical characteristics of the electrodes for LCEC and flow injection analysis of carbohydrates were evaluated. Improved performance in LCEC and flow injection analysis of carbohydrates is demonstrated in terms of sensitivity, reproducibility, stability and surface renewability. It was possible to get improved performance of the electrodes as well as adaptability of the electrodes for practical applications by employing highly dispersed catalyst particles through the electrode matrix and robust polymeric electrode matrix.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.12 no.9
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• pp.802-809
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• 2000

This study numerically analyzes the thermal and flow characteristics inside the header in PFHE(parallel-flow heat exchanger) by employing a three-dimensional turbulence modeling. The following quantities are examined by varying the injection angle of the working fluid, the location of entrance and the shape of entrance: flow nonuniformity, heat transfer rate, and flow distribution in each passage. The result shows that the degree of significance among the parameters affecting the header part is in the order of the injection angle, the shape of entrance, and the location of entrance. The result also indicates that heat transfer rates compared to the reference model are increased by about 152% for the angle of injection of -$20^{\circ}C$, by about 127% for the shape of entrance with right and left long rectangular form, and by about 108% for the location of entrance located at the lowest Position.

• Fibers and Polymers
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• v.2 no.4
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• pp.203-211
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• 2001

Residual stresses were predicted by a flow analysis in the mold cavity and residual stress distribution in the injection molded product was measured. Flow field was analyzed by the hybrid FEM/FDM method, using the Hele Shaw approximation. The Modified Cross model was used to determine the dependence of the viscosity on the temperature and the shear rate. The specific volume of the polymer melt which varies with the pressure and temperature fields was calculated by the Tait\`s state equation. Flow analysis results such as pressure, temperature, and the location of the liquid-solid interface were used as the input of the stress analysis. In order to calculate more accurate gap-wise temperature field, a coordinate transformation technique was used. The residual stress distribution in the gap-wise temperature field, a coordinate transformation technique was used. The residual stress distribution in the gap-wise direction was predicted in two cases, the free quenching, under the assumption that the shrinkage of the injection molded product occurs within the mold cavity and that the solid polymer is elastic. Effects of the initial flow rate, packing pressure, and mold temperature on the residual stress distribution was discussed. Experimental results were also obtained by the layer removal method for molded polypropylene.

• Proceedings of the KSME Conference
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• 2001.06c
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• pp.47-52
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• 2001

Micro-injection molding and microfluidic devices with the help of MEMS technologies including the LIGA process are expected to play important roles in. micro-system industries, in particular the bioapplication industry, in the near future. Understanding fluid flows in micro-channels is important since micro-channels are typical geometry in various microfluidic devices and mold inserts for micro-injection molding. In the present study, both experimental and numerical studies have been carried out to understand the detailed flow phenomena in micro-channel filling process. Three sets of micro-channels of different thickness were fabricated and a flow visualization system was also developed to observe the filling flow into the micro-channels. Experimental flow observations were extensively made to find the effects of channel width and thickness, and effects of surface tension and volume flow rate and so on. And a numerical analysis system has been developed to simulate the filling flow into micro-channels with the surface tension effect taken into account. Discussed are the flow visualization experimental observations along with the predictability of the numerical analysis system.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2011.11a
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• pp.116-121
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• 2011

Performance of Secondary Injection Thrust Vector Control system is investigated under various secondary injection operating conditions. 3-dimensional converging-diverging nozzle having 8 secondary injection nozzles is used in this numerical study. Total pressure of flow inside the nozzle is about 70bars, and total temperature set to 300K for cold flow simulation. Effect of secondary injection flow rate and injection nozzle configuration is considered in this research. Simulation is conducted with commercial CFD code Ansys Fluent v13. Spalart-Allmaras(1-equation)model is used for turbulence modeling with AUSM+ scheme. Various performance factors as Axial thrust, side force, system specific impulse ratio are considered and explained for system performance evaluation.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.11a
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• pp.293-296
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• 2007

Using the T3 free-piston shock tunnel in ANU, the cavity frequency and flow characteristics of no mass-injection, inclined mass-injection before the cavity, parallel or reverse mass-injection in the cavity are investigated in the case of Mach 3.7 inflow condition. No mass-injection doesn't have the harmonic frequencies but has high amplitude of pressure spectrum at 10 kHz. Inclined mass-injection attenuates the cavity flow fluctuation as disturbing the shear layer reflection at the trailing edge. Parallel mass-injection flow reflects at the trailing edge of the cavity directly hence, increases the cavity flow fluctuation at high injection pressure.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.23 no.6
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• pp.561-568
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• 2014

Injection molding industries realize the necessity of developing family molds to improve competitiveness. One of the primary causes of manufacturing defective products is the imbalance of flow in a family mold. In this study, the family mold of a shelf for refrigerators is analyzed by using CAE software. First, the flow balance, clamping force, and injection pressure are analyzed for different gate diameters of two cavities. Second, the flow balance, clamping force, and injection pressure are improved when the two gate valves are open at different times. Finally, the results of filling analysis are compared with the test injection product.

• Transactions of the Korean Society of Mechanical Engineers
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• v.17 no.7 s.94
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• pp.1841-1850
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• 1993

A simplified one-dimensional analysis has been performed to predict the local pressure distributions in Y-Jet twin-fluid atomizers. Fluid compressibility was considered both in the gas(air) and two-phase(mixing) ports. The annular-mist flow model was adopted to analyze the flow in the mixing port. A series of experiments also has been performed; the results show that the air flow rate increases and the liquid flow rate decreases with the increase of the air injection pressure and/or with the decrease of the liquid injection pressure. From the measured injection pressures and flow rates, the appropriate constants for the correlations of the pressure loss coefficients and the rate of drop entrainment were decided. The local pressures inside the nozzle by prediction reasonably agree with those by the experiments.

• KSBB Journal
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• v.19 no.3
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• pp.241-244
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• 2004

An automated flow injection analysis apparatus manufactured economically was used in the automatic measurement of E. col growth in shake-flask culture of nutrient broth. The detailed measurement of whole growth was possible at intervals of 10 minutes by the automatic measurement system which adopted the sterilized nutrient broth as a carrier solution. Using distilled water as a carrier solution showed less accurate results than nutrient broth.