• 한국식품과학회지
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• 제21권1호
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• pp.13-16
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• 1989

선택된 당류 및 전분 가수분해 물질을 기질로 사용하여 냉동건조에 의하며 acetaldehyde을 고정시키는 방법을 연구하였다. 단순기질 보다는 복합기질이 일반적으로 acetaldehyde를 고정시키는 능력이 좋았고. 특히 mannitol+lactose가 고정능력 면에서 가장 우수했다. 그리고 경계성 등을 고려할 때 mannitol+maltodextrin이 공업적으로 acetaldehyde를 고정하는데 적당할 것으로 평가됐다. 일반적으로, 기질 농도가 증가함에 따라 건조 중 acetaldehyde손실이 감소하였으며, 기질 농도 40%일 때 mannitol+maltodextrin 기질의 acetaldehyde 고정 능력이 가장 좋았다. 냉동건조 방법으로 acetaldehyde를 고정할 경우 건조실 압력이 낮을수록 건조 중 acetaldehyde 손실이 감소했다. 그러나 대량생산을 목적으로 할 때에는 0.5 Torr이하의 압력을 유지하는데 건조경비 부당이 과다하므로, 0.5 Torr정도의 건조실 압력을 유지하는 것이 적당할 것으로 평가됐다.

• 대한수의학회지
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• 제47권2호
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• pp.203-207
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• 2007

Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process. The microwave irradiation was also incorporated in the serial dehydration process with ethanol. The complete procedure from the initial fixation to the completion of dehydration with ethanol was reduced to 1 h with good preservation of the ultrastructural details of the specimens.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.230.1-230.1
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• 2015

One of the major problems of biological ToF-SIMS imaging is the lack of protein and peptide imaging. Most of biological story telling is mianly based on proteins. The biological implication of lipid ToF-SIMS imaging would be much higher if protein imaging is provided together. Utilizing high secondary ion yields of metals, proteins can be ToF-SIMS imaged with nanoparticle tagged proteins. Nanoparticles such as Fe3O4, SiO2, PbS were used for imaing NeuN, MCH, Orexin A, ${\alpha}$ synucline, TH(Tryosine Hydroxylase) in mouse tissues with a spatial resolution of ${\sim}2{\mu}m$ using a TOF-SIMS. Lipids and neurotransmitters images obtained simultaneously with protein images were overlayed for more deeper understanding of neurobiology, which is not allowed by any other bioimaging technqiues. The protein images from TOF-SIMS were compared with confocal fluorescence microscopy and NanoSIMS images. A new sample preparation method for imaging single cell membranes in a tissue using the vibrotome technique to prepare a tissue slice without any fixation and freeze drying will be also presented briefly for Hippocampus and Hypothalamus tissues.