• 농촌의학ㆍ지역보건
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• 제22권2호
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• pp.239-252
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• 1997

A questionnaire survey including stool examination for the parasite ova, skin test, and enzyme-linked immunosorbent assay (ELISA) of the liver fluke to find the factors related with the prevalence of the parasite infestation was done toward 118 residents living in three villages of Song-jung, Yi-jung, and Chim-gok of Okok-myun, Goksung County, South Cholla Province. The results are as follows : 1. The prevalence of Clonorchis sinensis ova by the stool examination was 46.6%. The sensitivity and specificity of skin test to egg-positive cases were 63.5% and 68.9%. The sensitivity and specificity of ELISA to egg-positive clonorchiasis were 100% and 34.9%. 2. The prevalence of Clonchiasis was higher in male than in female. 45~64 year old group was the highest among age groups, and the longer the duration of living in the present residence was, the higher the prevalence was shown statistically significantly (p<0.01). 3. The present drinker had higher prevalence of Clonorchiasis significantly than the past drinker or non-drinker, and the more the frequency of drinking the higher the prevalence was shown significantly (p<0.05). 4. Those who took raw fish had significantly higher than persons who didn't take it or take cooked fish(p<0.05) and prevalence showed the tendency to be increased as the frequency to take raw fishes increased significantly(p<0.05). 5. Those who had taken a raw sweet fish(Plecoglossus altivelis) or snake-headed fish(Ophicephalus argus) showed significantly higher prevalence of infection with liver fluke than persons who didn't take the raw fish. 6. While 56.4% of persons infected with Clonorchiasis answered that they were healthy, only 13.0% of the subjects were careful for their health, and 41.8% experienced tests to diagnose liver flukes before they were examined by this survey. 7. While 56.4% of infected persons thought the prevalence was high in their community, 54.0% were afraid to be infected, 47.1% thought the infection might destroy their health, 25.5% took treatment drugs, and 18.2% took raw fish continuously after treatment. The above results confirmed that Goksung area was highly prevalent in Clonorchiasis, so it was necessary to push effective preventive program against liver fluke infection together with the program to diagnose and treat existing infected persons.

• 한국수산과학회지
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• 제23권5호
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• pp.353-360
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• 1990

The bacterium Edwardsiella tarda was injected into healthy snakeheads (Channa argus) in order to prove the causative agent of ascite. The bacterium dominantly isolated from 2 cultured ascite snakeheads was injected into fish by the dose of $5\times10^6$ CFU/ 0.25ml, but the same dose of $0.65\%$ physiological saline was injected into the each control. The injected fish was divided into 4 groups such as intraperitoneal, intramuscle, control intra-peritoneal and control intramuscle according to their injection points. Each was composed of 10 healthy snakeheads respectively. Ascites and haemorrhagic ulcers became distinct 5 days after injection, but controls did not show any abnormal symptoms during the experimental period. Edwardsiella tarda was reisalated out of the injected fish's ascite, liver, kidney, spleen and haemorrhagic ulcer on the skin. Regardless of the injecting methods, liver was necrotized more severely than any other internal organ, but both the glomeruli of kidney and spleen were considerably damaged. Necrosis of muscle and a number of leucocytes were observed at the ulcerous region of the intramuscular injected fish. It is concluded that judging from the above results the Edwardsiella tarda is a causative agent to cause ascite in snakehead.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.149-158
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• 2013

We demonstrate enhanced growth, innate immunity and protection against hydrogen peroxide ($H_2O_2$)-induced protein oxidation and cellular DNA damage in olive flounder Paralichthys olivaceus fed a chitosan-coated moist pallet (MP) diet. A chitosan-based biopolymer coated MP as the experimental diet and a non-coated MP (control) was fed to olive flounder fish. Growth, including the average weight gain (g/fish), weight gain (%) and feed intake (g) of the fish group fed a chitosan-coated MP diet increased significantly. The survival rate was reported as 100% throughout the experimental period. Immunological parameters indicated higher mucus lysozyme activity and significantly higher fish skin mucus total protein content was observed in fish fed the chitosan-coated MP diet compared to the control. A blood plasma analysis revealed attenuation of cellular DNA and protein oxidative damage caused by $H_2O_2$-induced oxidative stress in the fish fed the chitosan-coated MP diet compared to the control group. Moreover, blood serum biochemical analysis revealed health-promoting effects, including significantly higher hemoglobin and total cholesterol levels in the fish fed the chitosan-coated MP diet compared to the control group. In conclusion, growth, innate immunity and protection against oxidative stresses were improved by feeding of the chitosan-coated MP diet to olive flounder reared in aquaculture.

• 한국어병학회지
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• 제24권3호
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• pp.213-223
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• 2011

충청남도 보령시 인근연안에서 어획된 문치가자미, Pleuronectes yokohamae 10개체 중에서 6개체가 피부에 육아종이 형성되었다. 기생충과 병원균으로 의심되는 세균은 증식하지 않았으며, 환부의 균질액을 CHSE-214 세포에 접종하였으나 CPE가 관찰되지 않았다. 환부를 광학현미경으로 관찰하였을 때 상피 세포의 증식으로 상피층은 정상개체와 비교하였을 때 유의적인 차이를 보이며 두꺼워졌고, 육아종 주름이 형성되었다. 비대되어진 상피세포는 핵융해 및 뚜렷하게 관찰되는 인이 특징이었으며, 세포질은 혼탁하였다. 이들 상피층에는 원형의 작은 핵과, 뚜렷한 인이 관찰되는 X-cell이 존재하였다. 진피층에는 다수의 신생 혈관들이 존재하고 있었다. 점액세포의 크기는 정상개체와 비교하였을때 유의적인 차이를 보이며 증가하였다. 본 연구에서는 병원체가 확인되지 않았지만 추가적인 실험을 통해서 원인체를 확인해야 할 것으로 생각된다.

• 미생물학회지
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• 제31권2호
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• pp.113-122
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• 1993

본 논문은 양식장에서 자연감염된 궤양성 가물치로부터 우점종으로 분리된 Aeromonas veronii 를 건강한 가물치에 접종한 후, 어체 각 기관의 병변 진행사항을 조직학적 측면에서 연구한 것이다. 양식장내에서 자연 간염된 어체에서의 병변과정은 관찰할 수 없었지만, Aeromonas veronii 로 인공감염시킨 어체의 병변은 자연상태에서 감염된 병변과 매우 유사하였다. 인공감염시킨 어체는 감염후 2일째부터 피부를 비롯하여 아가미, 소화관 조직에 뚜렷한 조직괴사가 식별되었고, 5일 후에는 신장, 사망 직전인 9일 후에는 간과 비장까지 조직의 괴사내지는 세포응축을 나타냈다. 병이 진행됨에 따라 가물치는 피부손상을 비롯하여 호흡, 소화흡수, 배설 등 영양대사의 저해를 수반하면서 감염후 9일이내에 사망하는 것으로 나타났다. 자연감염 어체에서 우점종으로 분리된 균주, Aeromonas veronii 는 가물치의 출혈성 궤양증을 유발하는 주된 균증의 하나로 판단된다.

• 한국축산식품학회지
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• 제41권4호
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• pp.674-686
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• 2021

This study aimed to enhance the quality of broiler breast meat by adding pig skin collagen to feed. A total of 50 Ross 308 broilers were classified according to the following feeding regime for two weeks: basal diet (NC), basal diet+0.1% fish collagen (PC), basal diet+0.1% pig skin collagen (T1), basal diet+0.5% pig skin collagen (T2), and basal diet+1.0% pig skin collagen (T3). The moisture content was the highest in the PC group, and the protein content was the lowest in the T1 group (p<0.05). The fat content was higher in the T1 and PC groups, whereas the ash content was higher in the T3 group (p<0.05). Drip loss was the highest in the NC group and the lowest in the T2 group (p<0.05). Lightness was low in groups T2 and T3, redness was low in groups T2 and PC, and yellowness was low in groups T1, T2, and PC (p<0.05). The collagen content of the chicken breast was the highest in the T3 group, and that of the skin was the highest in the T1 group (p<0.05). The texture characteristics of springiness, cohesiveness, chewiness, and hardness were the highest in the T3 group (p<0.05). In conclusion, the supplementation of a broiler diet with pig skin collagen was found to increase the collagen content of the breast meat, indicating the improved quality of the broiler breast meat.

• 한국발생생물학회지:발생과생식
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• 제18권2호
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• pp.79-87
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• 2014

In Pleuronectiformes, blind-side malpigmentation (hypermelanosis) is common in cultured flatfishes, and is economically important. To understand the mechanism of blind-side hypermelanosis in flatfishes, we examined when the malpigmentation initially occurred, and studied how the symptoms proceeded during early development of the starry flounder, Platichthys stellatus. To assess quantitative pattern changes of blind-side skin, we observed morphological development of the whole body from 22 (total length [TL] $10.0{\pm}0.2$ mm and body weight [BW] $8.8{\pm}0.57$ mg) to 110 days (TL $23.4{\pm}0.7$ mm, BW $193.6{\pm}23.3$ mg) after hatching (DAH), and also examined the malpigmented area rate of blind-side skin and the malpigmented fish ratios. The experimental animals were reared in fiberglass-reinforced plastic (FRP) tanks in water at a temperature of $18.9{\pm}1.9^{\circ}C$ and salinity of $32.6{\pm}0.6$ psu and were fed with rotifer and Artemia nauplii from 22 to 48 DAH, and with A. nauplii and commercial feed from 49 to 110 DAH. As results, the first staining patch seen by the naked eye was observed around the area between the anus and pelvic fin or caudal edge of the trunk at 80 DAH (TL $20.6{\pm}0.5$ mm, BW $112.5{\pm}8.8$ mg). The pigmented area and the pigmented fish ratios were significantly increased from 80 to 110 DAH. These results indicated that malpigmentation on the blind side of starry flounder was initially observed at about 2 cm in length and 100 mg in weight, and the pigmented domain on the blind-side skin was continually broadened by the differentiation of pigmented cells (melanophores and xanthophores) with growth.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.46-50
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• 2000

우리나라에서 유통되고 있는 자주복의 독성을 조사하기 위하여 1995년 1월, 4월 및 9월에 부산시내 자갈치 어시장에서 총 27개체를 구입하여 부위별 독성을 조사하였다. 간장의 경우 독성이 10MU/g 이상인 유독개체 출현율은 14.8%, 최고 독성치는 160 MU/g, 평균 독성치는 7$\pm$6 MU/g(평균치$\pm$표준오차)이었다. 생식소의 경우는 각각 16.7%, 600 MU/g 및 50$\pm$35 MU/g 이었고, 껍질에서는 각각 14.8%, 26 MU/g 및 5$\pm$1MU/g이었다. 그러나 근육에서는 독이 검출되지 않았다. 계절에 따른 독성은 1월 및 4월의 일부시료에서는 유독부위가 있었으나 9월에는 시료어의 전부위에서 독성이 검출되지 않았다. 자주복의 독성은 부위나 계절에 따른 차이가 있지만 주 가식부인 근육과 껍질에서는 거의 독이 검출되지 않거나 약한 독성이 검출되어 식품 위생상 비교적 안전한 종으로 판단되었다.

• 한국어병학회지
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• 제11권2호
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• pp.119-127
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• 1998

기생성 요각류 2종, Clavellopsis hugu(Lernaeopodidae; Siphonostomatoida)와 Taeniacanthus yamagutii(Taeniacanthidae; Poecilostomatoida)이 한국산 양식 황복의 구강, 코, 표피 등에 기생하고 있음을 국내에서 처음으로 보고하며, 아울러 이종의 형태학적 특징을 상세히 기술하였다. Clavellopsis huge의 체형, 제2 촉각, 큰 턱 및 제2 소악 등은 다른 종과 쉽게 구별된다. 암컷의 두흉부는 동쪽으로 휘어져 있으며, 제2 촉각은 2분지, 큰 턱의 날은 톱니 모양, 제2 소악은 두흉부 기저에 완전히 융합될 bulla 형태이다. 수컷의 두흉부와 몸통은 완전히 융합된 난형이며, 두쌍의 악각은 집게형이다. Taeniacanthus yamagutii의 주요 특징은 제2소악이 견고하고, 악각의 갈고리 기저에 돌기가 있으며, 제2-4 바깥 다리의 가시 끝에 촉각이 1개씩 있다. 이들은 모두 한국 미기록종이며, 양식산 복어(Takifugu obscurus)는 새로운 숙주이다.

• 생명과학회지
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• 제25권3호
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• pp.269-275
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• 2015

생체에서 가장 많은 비율로 분포하고 있는 콜라겐 펩타이드는 동물의 뼈와 해양 생물의 비늘에 많이 함유되어 있다. 콜라겐은 동물 생체의 여러 결합조직에서 구조 단백질로 흔하게 발견된다. 또한, 이들은 생물 의료 자재, 제약, 화장품, 식품 및 가죽 산업에 널리 이용된다. 각종 어류 비늘에서 추출된 펩타이드는 UVB 조사에 의해 유도된 피부의 손상 및 광 노화에 대한 보호 효과가 있었다. 그럼에도 불구하고, UVB 조사에 대한 옥돔 비늘 유래의 펩타이드 특성은 명확히 알려져 있지 않다. 이 연구에서는 옥돔 비늘 추출물에서 분리된 1 kDa 이상(HMP)과 1 kDa 이하(LMP)의 펩타이드를 이용하여 UVB 조사에 의해 유도된 피부 손상과 광 노화에 대한 효과를 연구하였다. 이들 펩타이드는 농도 의존적으로 DPPH 라디칼 소거능을 보였으며 LMP는 HaCaT 인간 피부세포에서 UVB 조사에 의해 유도된 세포 지질 과산화 산물인 8-isoprostane 생성을 억제하였다. 그리고 LMP와 HMP는 B16F10 마우스 흑색종 세포에서 tyrosinase 활성 및 melanin 함량을 감소시켰으며 또한 HaCaT 세포에서 UVB로 유도된 elastase 활성을 감소시켰고 matrix metalloproteinase-1의 활성을 감소시켰다. 이러한 결과는 옥돔 비늘에서 유래된 펩타이드가 미백효과, 항산화제 및 광 노화 억제제로서 유용한 물질이 될 것으로 기대된다.