• 한국수산과학회지
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• 제46권5호
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• pp.534-539
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• 2013

To secure the biological safety of live fish and shellfish for raw consumption, the germicidal effects of electrolyzed seawater were evaluated. Upon direct exposure to electrolyzed seawater, coliform group bacteria were killed and decreased to undetectable levels after 1 day. The physicochemical characteristics of the seawater were stable during the test period. A byproduct of chlorine disinfection, trihalomethane, was not generated by the electrolysis of seawater. Vibrio parahaemolyticus infection in a live fish was effectively resolved by electrolyzed seawater and became undetectable after 12-36 h of treatment. Bioaccumulation of coliform group and fecal coliform bacteria in live oysters Crassostrea gigas was removed within 18 h of treatment. This study demonstrated that electrolyzed seawater is an effective and safe germicidal agent for the traditional retail market and can help to prevent outbreaks of foodborne disease associated with the consumption of raw fish and shellfish.

• Journal of the korean society of oceanography
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• 제37권2호
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• pp.82-89
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• 2002

Butyltins (BTs) were measured in 2 species of shellfish and 16 species of fish collected along the Korean coast. Tributyltin (TBT) was detected in the mussels and oysters of sentinel organisms (20 to 940 ng Sn/g dry wt), indicating widespread contamination of TBT in the Korean coast. The elevated concentrations of TBT in the shellfish found in the sites near harbors or shipyards suggested that antifouling paints are probable major sources of butyltins in these areas. The TBT compound was detected in 12 out of 16 fish samples. The concentrations of TBT in fish muscles wee between 7 and 151 ng Sn/g dry wt, while the level in whole body of anchovy was very high (793 ng Sn/g dry wt). Exposure doses to Korean people via consumption of these marine products wee evaluated. The results suggested that the environmental levels of TBT were below the level of concern.

• 한국수산과학회지
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• 제54권4호
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• pp.388-396
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• 2021

Antimicrobial resistance patterns of Escherichia coli were investigated. Strains were isolated from 310 shellfish, 36 crustaceans, and 12 fish collected off the West Coast of Korea from April 2019 to October 2020. Two hundred and ninety-five E. coli strains were isolated from shellfish, 100 from crustaceans, and 54 from fish. Strains isolated from shellfish showed the highest resistance to ampicillin (27.5%), whereas those from crustaceans were resistant to sulfisoxazole (30.0%) and those from fish were resistant to ampicillin (59.3%) and sulfisoxazole (59.3%). Ceftazidime resistance was observed in strains isolated from short neck and hard clams, whereas gentamicin resistance was observed in strains from fish. Multi-drug resistance was observed in 56 strains (48.7%) isolated from shellfish, 11 (28.2%) from crustaceans and 27 (73.0%) from fish. Depending on the source of isolation, the strains showed specific antimicrobial resistance tendency. Strains isolated from shellfish showed 12 different multi-drug resistance patterns, whereas those from crustaceans showed high resistance (59%) to a single antimicrobial agent and those from fish showed a broad trend of multi-drug resistance to more than eight antimicrobials.

• 한국식품위생안전성학회지
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• 제14권3호
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• pp.305-318
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• 1999

식품의 안전성을 확보하고 건강상의 위험을 방지하기 위하여 또 수산 어패류 중의 중금속 분포상태를 파악하기 위해 국내에서 유통되고 있는 민물어류 11종 166건, 패류 14종 153건 등 총 319건을 대상으로 Pb, Hg, Cd, Cr, Fe, Mn, Zn, Cu 의 8개 금속을 A.A.S. 와 Mercury Analyzer로 측정하여 다음과 같은 결과를 얻었다. 1. 민물어류에서의 중금속 평균함량은 Pb 0.075, Hg 0-.053, Cd 0.002, Cr 0.135, Fe 8.695, Mn 1.078, Sn 9.491, Cu 0.548 mg/kg으로 나타났다. 패류에서의 중금속 평균 함량은 Pb 0.059, Hg 0.007, Cd 0.146, Cr 0.147, Fe 40.808, Mn 7,738, Zn 13.943, Cu 2.731 mg/kg으로 조사되어, Cd , Fe, Mn, Cu 등이 민물어류에 비해 높은 것으로 나타났다. ()P<0.001). 2. 중금속 함량은 산지에 따라 유의한 차이가 있는 것으로 나타났다. 민물어류의 경우 Mn 과 Zn 함량이 지역간의 유의성을 나타냈다. (p<0.001). 패류의 경우는 Fe, Mn, Zn 함량이 지역간의 유의성을 나타내었다. (p<0.01). 3. 계절에 따라 중금속 함량에도 차이가 있는 것으로 나타났다. (p<0.05). 민물어류에서는 겨울철의 Pb, Zn, Cr, Cu 함량이 높은 것으로 나타났고, 패류에서는 겨울철에 Pb, Zn 함량이 높은 것으로 나타났다. 4. 조사된 결과를 종합 고찰하면 우리나라 서식하고 있는 어패류에 함유된 중금속 함량은 유해농도이하이며 , Fe, Mn, Zn 등 미량 영양염류 함량은 비교적 높은 것으로 나타났다.

• Food Science and Biotechnology
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• 제18권4호
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• pp.842-848
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• 2009

The purpose of this study was to determine the extent of domoic acid (DA) a potent neurotoxin, responsible for the syndrome amnesic shellfish poisoning (ASP) contamination of various species of bivalve shellfish purchased from fish market in Korea and the implications for food safety. Liquid chromatography (LC) methods were applied to quantify DA in shellfish after sample clean-up using solid-phase extraction (SPE) with strong anion exchange (SAX) cartridges. Toxin detection was achieved using photodiode array ultraviolet (LC-UV) and electrospray ionization-mass (LC-ESI-MS). DA was identified in 4 bivalve shellfishes of 872 shellfishes collected from March, 2006 to October, 2007 in Korea. DA amount of 3 surf clams (Mactra veneriformis) collected at Seoul, Daejeon, and Daegu were 4.13, 1.99, and 1.94 mg/kg, respectively. DA amount of 1 pink butterfly shell (Peronidia venulosa) collected at Seoul was 3.02 mg DA/kg. The amounts of DA that were present in 4 bivalve shellfishes were within EU guideline limits for sale of shellfish (20 mg DA/kg).

• 한국어병학회지
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• 제34권2호
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• pp.133-140
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• 2021

With the recent isolation of a new betanodavirus in shellfish, Korean Shellfish Nervous Necrosis Virus (KSNNV), it has also been identified the reassortant KSNNV of two RNA segments, in which one segment is KSNNV genotype but the other one is known genotype. In this study, we confirmed that the ressortant KSNNVs obtained in previous screening study of our laboratory for betanodaviruses in shellfish were KS/RGNNV and RG/KSNNV type by performing two consecutive multiplex RT-PCR on each RNA1 and RNA2 segment (R1- and R2-discriminative multiplex two-step RT-PCR, respectively) to determine the genotype of each segment based on the size of amplicon. In the pathogenicity analysis, none of the reassortants induced specific external symptoms or mortality of VNN, but viruses of 2 × 10⁴~10⁵ copies/mg or more were detected at 14 days after injection (10⁷ copies/fish) in brain tissues of 4 species except for crucian carp and common carp among the 6 species of juvenile fish used. In addition, the histopathological features of weak but distinct vacuole formation were also found in the brain of these infected fish, but no difference was found between the two reassortants KS/RGNNV-KG and RG/KSNNV-CM.

• 한국수산과학회지
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• 제23권1호
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• pp.51-60
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• 1990

Some species of dinoflagellates were considered as one of the causative organisms of PSP(Paralytic Shellfish Poison) or DSP(Diarrhetic Shellfish Poison). Fish and shellfish are intoxicated by feeding of toxic plankton, sometimes human is intoxicated by feeding on these intoxicated fish and shellfish. In past ten years, the physiological and ecological studies of the toxic plankton has been investigated for development of monitoring system and preventation and control measures of PSP. However, in our country still little is known on a research for the toxic dinoflagellates. This paper reviews the general biology, taxonomic problem, physioecology and culture method of the toxic planktons such as Protogony-aulax and Dinophysis.

• 한국식품위생안전성학회:학술대회논문집
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• 한국식품위생안전성학회 1998년도 춘계학술세미나 및 학술발표회 - 최근 문제가 되고 있는 식중독의 연구 동향
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• pp.19-33
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• 1998

• 해양환경안전학회지
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• 제21권1호
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• pp.109-118
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• 2015

연안에서 발생하는 부유물질에 의한 해양생물영향을 평가하기 위한 기초자료를 제공하기 위하여, 유용 해양생물(패류, 어류, 해조류 등)에 대한 부유물질의 영향에 관한 문헌을 수집하고 분석하였다. 1) 부유물질에 의한 해양생물들의 영향에 관한 논문은 주로 패류에 대해서 이루어져 있다. 2) 해양생물에 대한 부유물질의 영향은 주로 급성독성에 대해서 조사가 이루어져 있다. 3)부유물질에 의한 영향 실험은 성패와 치패기의 패류, 난 및 치어기의 어류에 집중되어 있으며, 해조류는 포자를 이용한 연구가 주로 이루어졌다. 부유물질에 의한 생물학적 영향은 부유물질의 농도, 노출기간, 생물의 성장단계에 따라 다양하게 나타날 것이며, 이에 대한 검토는 부유물질의 영향에 대한 이해를 향상시킬 것이다.

• 한국수산과학회지
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• 제21권2호
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• pp.80-84
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• 1988

방어, 참돔, 피조개, 굴 4종의 어패육에 V. vulnificus M-8을 접종하여 저장온도와 시간에 따른 균수의 변화를 시험한 결과는 다음과 같다. 1. V. vulnificus의 저온저항성은 $-20^{\circ}C$의 $1.5\%$ 식염-인산완충희석수에서는 32시간에 거의 사멸하였으며, 방어육 균질액에서는 인산완충희석수에서 보다 감소가 완만하였고 72시간 이후에도 균이 검출되었다. 그리고 $4^{\circ}C$ 저장에서도 균의 감소가 현저하였다. 따라서 이 균의 저온저항성은 약하지만 단시간 냉동 및 냉장에 의한 균의 사멸은 어렵다는 것을 알 수 있었다. 2. 어패육에 접종하여 $30^{\circ}C$에 저장한 시료중의 V. vulnificus의 증식은 일반적으로 16시간만에 최대균수에 달하였으나 피조개의 경우는 12시간으로 약간 빨랐으며 특히 방어육에 있어서는 초기에 감소하는 특징과 더불어 균의 증식속도가 완만하였다. 그리고 가열처리육보다 생육에서의 균의 증식이 빠르게 나타났다.