• IMMUNE NETWORK
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• 제8권4호
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• pp.124-129
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• 2008

Background: The immunomodulatory effects of Korean mistletoe (Viscum album Coloratum) on the innate immune responses of eel (Anguilla japonica) were studied. Methods: Mistletoe, Freund’s complete adjuvant (FCA), or phosphate-buffered saline (PBS) as a control was injected into eel peritoneal cavities. Results: Nitroblue tetrazolium (NBT)-positive cells in the head kidney of eel were significantly augmented by the second day post-injection of mistletoe. Reactive oxygen intermediates (ROI) were more produced in mistletoe-injected fish kidney leucocytes than in FCA-injected ones. The level of lysozyme activity in the serum of fish 2 days after injection with mistletoe was also significantly higher than that in the serum of the control fish. The optimal concentration of mistletoe in inducing the highest serum lysozyme activity was revealed to 500${\mu}$g/200 g of fish. In phagocytic activity assay, mistletoe-sensitized eel kidney phagocytes captured more zymosan than did the control fish. Conclusion: Korean mistletoe appeared to be a good activator of the non-specific immune responses of eel.

• 한국어병학회지
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• 제18권1호
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• pp.59-66
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• 2005

In the present paper, the immunostimulatory effects of Korean mistletoe (Viscum album Coloratum) on the non-specific immune responses of Japanese eel (Anguilla japonica) were examined. Eel were innoculated with mistletoe, Freund's complete adjuvant (FCA), or phosphate-buffered saline (PBS) as a control into their peritoneal cavities. The number of nitrobule tetrazolium (NBT)-positive cells in the head kidney of fish was significantly increased by the second day post-injection of mistletoe. ROI products were more enhanced in mistletoe-injected fish kidney leucocytes than in FCA-injected ones. The level of lysozyme activity detected in the serum of fish 2 days after injection with mistletoe was also significantly higher than that found in the serum of the control fish. The appropriate concentration of mistletoe to induce the highest level of serum lysozyme activity was revealed to 1000${\mu}g$/200 g of fish. In phagocytic activity assay, mistletoe-sensitized eel kidney phagocytes captured more zymosan than did the control fish. Korean mistletoe appeared to be a good activator of the non-specific immune responses of Japanese eel.

• 한국어병학회지
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• 제2권2호
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• pp.83-90
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• 1989

Edwardsiella tarda, the causative organism of Edwardsiellosis, was isolated from the water and the eel, Anguilla japonica of the recirculating eel culture ponds in 1988. E. tarda was always isolated from the water of eel culture ponds in the number of log 2.30~log 4.78 CFU/$m{\ell}$ without distinguishable seasonal characteristics. The counts of isolated E. tarda were changed with the health condition of the eel, i. e., a number of E. tarda were isolated from all checked organs, intestine contents, liver, spleen and kidney of the diseased eel, however, it was hardly isolated from spleen and kidney of the healthy eel. The isolates were classified according to o-agglutinating test into three serotypes (I, II, III). There were no distinct differences in the composition of the serotypes between intestine contents of eel and pond water samples(I, 52.4~53.3% ; II, 20.0~23.8% ; III, 4.8~6.7%). Serotype I was ascendant in the spleen(66.7%) and the kidney(73.7%) samples. By experimental challenge, most of serotype I proved highly virulent to eel in comparison with the other serotypes tested.

• 한국어병학회지
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• 제36권2호
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• pp.287-292
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• 2023

This study investigated the genetic determinants of plasmid-mediated antibiotic resistance (PMAR) to quinolones and tetracycline in 106 Aeromonas strains isolated from eel (Anguilla japonica, 70 strains) and ornamental fish (36 strains) in Korea. Quinolones and tetracycline resistance phenotypes were found to be widely distributed throughout the both fish groups. However, the prevalence of qnr and tet genes was higher in ornamental fish strains than in eel strains (42.9% vs. 86.1% for qnr and 51.4% vs. 69.4% for tet). In addition, the profiling of the present genetic determinants revealed the dominance of qnrS, tetA, tetE and tetE+qnrS genes for eel strains but of tetA+qnrS qnrS and tetE+qnrS genes for ornamental fish strains. These results indicate that aquaculture and related industries could be a major threat to public health due to the possible spread of PMAR.

• 한국물환경학회지
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• 제29권5호
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• pp.656-665
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• 2013

This research was conducted to investigate pollutant load unit factor from aquacultural farms. Pollutant load unit factors were investigated on the 13 kinds of fish type, i.e., Trout, Leather Carp, Eel, Carp, Cartfish, Freshwater Eel, Crucian Carp, Colored Carp, Sturgeon, Marsh Snail, Sweetfish, Pond Snail, Eriocheir Sinensis. Water qualities in aquacultural farms were investigated wide range of values by fish type and pollutant items. High BOD and COD values were observed at the Leather Carp (Ponded water system), Cartfish (Ponded water system) and Freshwater Eel (Recirculating System). TOC and DOC values were relatively high at the Freshwater Eel (Recirculating System) and Eriocheir Sinensis (Extensive). Eel (Ponded water system) and Freshwater Eel (Recirculating System) produced high concentrated nutrient pollutants, i.e., T-N, T-P. Pollutant load unit factors are dependent on fish type, aquacultural type, water quality items, etc. If some fishes have similar basic unit values, those could be applied for TMDLs as a group of fish having same pollutant load unit. The water quality concentrations of post treatment facilities' (mainly reservoir tank) effluent were higher than those of fish raising bath because of extracting pollutant from sediment in the reservoir tank. Therefore, it needs to the management and regulations about post treatment facilities.

• 대한수의학회지
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• 제39권2호
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• pp.365-369
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• 1999

A study on quinolone antibacterial, norfloxacin, was performed to apply for the control of bacterial diseases in eel (Anguilla japonica). Norfloxacin was proved excellent in antibacterial activity and sensitivity against fish bacterial pathogens when compared with the existing antibacterials and antibiotics. And any side effect was not observed during the period of indicated use. An outline of minimal inhibitory concentration was $0.03{\sim}0.1{\mu}g/ml$, $TLm_{48h}$ value was 3,500mg/l. The residual time of the day in fish body was less than 17 days and any pathological changes were not observed. The study has revealed that norfloxacin can be applied to treat some fish bacterial disease by the dosage of 100g/day/ton of fish body weight for about 3 days perorally. Further, norfloxacin may be used for the control of bacterial pathogens in eel.

• 수산해양교육연구
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• 제26권6호
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• pp.1209-1216
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• 2014

This feeding experiment was conducted to investigate the effects of slurry type diet on growth performance and survival rate of growing leptocephalus, eel larva. We need to find new materials of diets for rearing eel larvae. Test diets were formulated with the eggs of the shark, fish soluble concentrate, soybean peptide and fish protein hydrolysate. Fish (average length 6 mm) were fed 3 slurry type diet(A, B and C) based on shark egg for 5 times per day. During feeding experiment, survival rates were significantly different among 3 slurry type diets. Total protein, lipid, moisture, ash and free amino acids contents were analyzed for slurry type diets. Leptocephalus fed the C slurry type diet was grown up to $38.0{\pm}9mm$ at 150 days. But all leptocephalus fed B slurry type diet were died at 100 days, reaching $16.4{\pm}8mm$. This results suggest that basic information for diet development of eel leptocephalus.

• 한국수산과학회지
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• 제47권6호
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• pp.860-868
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• 2014

The study was performed to investigate changes in hematological responses and antioxidative enzyme activities (superoxide dismutase, SOD; catalase, CAT) of Japanese eel Anguilla japonica following exposure to 0 (control), 2.33, 4.60, 6,64 and 8.78 mM nitrite-N in fresh water for 48 h. Hematological parameters such as plasma nitrite, electrolytes, cortisol, glucose, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), hemoglobin (Hb), methemoglobin (metHb) and NADH-methemoglobin reductase (NMR) were measured. Plasma nitrite, cortisol, metHb and NMR increased directly with increasing ambient nitrite concentration, while Hb content showed a progressive decline. Levels of plasma potassium, GOT and GPT of the eel exposed to 6.64 mM ambient nitrite were significantly higher than the control fish. The activity of SOD and CAT in plasma, gill and liver of the eel following exposure to nitrite were augmented by increasing ambient nitrite. Levels of plasma nitrite, metHb, NMR, cortisol, glucose and antioxidative enzyme activities of the eel exposed to 2.33 mM ambient nitrite were significantly higher than the control fish. This study suggested that the eel acutely exposed to elevated ambient nitrite causes nitrite-induced stress responses, changes in antioxidative enzyme activities and hematological parameters.

• 한국수산과학회지
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• 제18권2호
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• pp.139-148
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• 1985

잉어와 붕장어의 어체 전처리 방법에 따른 동결저장중의 어묵형성능에 관하여 실험한 결과는 다음과 같다. 어체 전처리방법으로써 전어체, 채육기로 채육한것, 채육하여 수세탈수한 것 및 고기풀의 4종 상태로 처리하여 $-30^{\circ}C$에서 3개월 동안 동결저장했을때 원료 및 제품의 품질은 모두 전체적으로 양호한 편이었다. 어체 전처리방법에 따라 처리하여 동결저장한 원료로써 만든 어묵제품 품질은 잉어의 경우, 전어체 그대로 동결저장하는 것이 제품 품질이 가장 양호하였고 그 다음이 채육기로 채육한 것, 채육하여 수세탈수한 것 및 고기풀의 순이었으며, 붕장어에서는 고기풀 상태로 저장한 것이 가장 좋았으며 다음이 전어체, 채육하여 수세탈수한 것, 채육기로 채육한 것의 순이었다.

• 한국수산과학회지
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• 제34권6호
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• pp.678-684
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• 2001

우리나라에서 생선회로 즐겨 식용하고 있으나, 형태, 색조 및 맛이 유사하여 소비자들로부터 구분이 명확하지 않은 붕장어와 갯장어의 횟감으로서 식품성분 특성 (일반성분, 영양특성 및 조직 특성)을 비교 검토하였다. 생선회로서 붕장어는 갯장어에 비하여 수율 및 회분의 경우 약간 높았고, 기타 일반성분은 차이가 없었다. 주요 구성지방산은 붕장어회의 경우 16:0 ($19.9\%$), 18:1n-9($36.1\%$) 및 22:6n-3 ($8.3\%$) 등이었고, 갯장어회의 경우 16:0 ($24.0\%$), 16: 1n-7 ($12.1\%$) 및 18:1n-9 ($21.9\%$) 등이었으며, n-3계 지방산 조성은 모두 낮았다. 붕장어회 및 갯장어회의 총 아미노산 함량은 각각 19,147.2mg/100g 및 18,527.1 mg/100g으로 차이가 없었고, 이들을 구성하는 주요 아미노산은 장어류의 종류에 관계없이 aspartic acid ($11.7\%$ 및 $11.8\%$), glutamic acid ($17.1\%$ 및 $17.0\%$ )와 같은 산성 아미노산과 곡류 제한아미노산인 lysine ($11.6\%$ 및 $11.9\%$) 등으로 나타났다. 이들을 토대로 계산한 단백질의 화학가는 붕장어회가 갯장어회에 비하여 약$6\%$ 높았다. 무기질 함량은 붕장어회가 갯장어회에 비하여 칼슘 및 인 함량의 경우 높았으나 기타 성분의 경우 차이가 없었다. 맛성분, 색조, 콜라겐 함량 및 관능검사의 결과로 미루어 보아 붕장어회가 갯장어회에 비하여 맛 및 조직감의 경우 열악하였고, 색조의 경우 차이가 없었다. 이상의 결과로 미루어 보아 생선회로 식용하는 경우 붕장어 회가 갯장어회에 비하여 영양 특성은 우수하였으나, 맛 및 조직감은 열악하였으며, 색조는 차이가 없었다.