• Title/Summary/Keyword: final pH

Search Result 1,043, Processing Time 0.024 seconds

Effects of Sucrose level and Nitrogen Source on Fresh Weight and Anthocyanin Production in Cell Suspension Culture of 'Sheridan' Grape (Vitis spp.)

  • Kim, Seung-Heui;Kim, Seon-Kyu
    • Journal of Plant Biotechnology
    • /
    • v.4 no.1
    • /
    • pp.23-27
    • /
    • 2002
  • To establish an in vitro mass production system of grape anthocyanin pigments through callus and cell suspension culture, the effects of nitrogen source and sucrose on fresh weight and anthocyanin production in cell suspension culture of 'Sheridan' grape level were studied. When the medium was devoid of $NO_3^-$, cell fresh weight was either remained stable (1% sucrose) or slightly decreased with culture time (2,3, and 4% sucrose). When $NH_4^-$ was lacking, 3% sucrose was most favorable for cell growth. When $NH_4^-$ was supplied as N source, the anthocyanin content of 2% sucrose containing medium was maintained 2 times higher than other levels till day 8 in culture, then that of 3 and 4% sucrose which peaked at day 12 thereafter. The anthocyanin content was low than $NO_3^-$-free media. Total anthocyanin content in $NH_4^-$-free medium was just about a half of that of $NH_4^+$ medium. Anthocyanin production of 2% sucrose in $NH_4^+$ medium was maintained about 3-fold till day 8, then decreased thereafter. In $NH_4^+$ medium, pH decreased gradually with final pH of 3.5 to 4.0, while pH in $NH_4^+$-free medium increased with final pH of 6.5 to 7.5.

Characterization of Heavy Metals Bioleaching from Fly Ash by a Sulfur-Oxidizing Bacterium Thiobacillus thiooxidans: Effect of Solid Concentrations (황산화세균 Thiobacillus thiooxidans에 의한 fly ash의 중금속 제거 특성:고형물 농도의 영향)

  • 조경숙;문희선;이인숙
    • Korean Journal of Environmental Biology
    • /
    • v.17 no.2
    • /
    • pp.183-190
    • /
    • 1999
  • The bioleaching of heavy metals from fly ash was performed by Thiobacillus thiooxidans MET isolated from the enrichment culture of an anaerobically digested sludge. The effect of solid concentrations on the efficiency of metal leaching was studied in shaken flasks. In the range of solid concentrations 20 g.L­$^1$to 100 g.L­$^1$T. thiooxidans MET oxidized S$^{0}$ to sulfate without any lag period. The final pH of slurry solution was decreased to below pH 1, and the final oxide-redox potential (ORP) was increased to over 420 mV in the solid concentrations below 100 g.L­$^1$. However, the initial lag period of 4 to 8 days was required to obtain the pH reduction and ORP increase of the slurry solutions in the range of solid concentrations 150 g.L­$^1$to 300 g.L­$^1$. The sulfur oxidation rate of T. thiooxidans MET in 20~100 g.L­$^1$solid concentrations was 0.70~0.75 g-S.L­$^1$ㆍ d­$^1$, but its sulfur oxidation activity was remarkably inhibited with increasing solid concentration over 150 g.L­$^1$. Increasing fly ash solids concentration in the range of solids concentration 20 g.L­$^1$ to 200 g.L­$^1$decreased the removal efficiency of Zn, Cu, Mn, Cr and Pb. The solubilization of heavy metals from fly ash was strongly correlated with the pH value of slurry solution. When the pH of slurry solution was reduced to 3, the solubilization process of Zn, Cu and Mn started, and their solubilization efficiency of Zn, Cu and Mn was progressively increased below pH 2. However, the solubilization process of Cr and Pb started at pH 2.5 and 2.0, respectively.

  • PDF

Cinnamic Acid Derivatives IV, The Kinetics and Mechanism of the Hydrolysis of Cinnamylidene aniline Derivatives (신남산 유도체 Ⅳ, Cinnamylidene anilin 유도체의 가수분해 반응에 대한 메카니즘과 그 반응속도론적 연구)

  • Lee, Gi-Chang;Park, Su-In;Hwang, Yong-Hyeon;Lee, Gwang-Il;Choe, Bong-Jong;Jeong, Deok-Chae
    • Journal of the Korean Applied Science and Technology
    • /
    • v.8 no.1
    • /
    • pp.1-7
    • /
    • 1991
  • The kinetic of hydrolysis for cinnamylidene aniline derivatives has been investigated by ultraviolet spectrophotometry in 20% (v/v) dioxane - $H_2O$ at $25^{\circ}C$. A rate equation which can be applied over wide pH range was obtained. The substituent effects on cinnamylidene aniline derivatives were studied and the hydrolysis was facilitated by electron attracting group. Final products of the hydrolysis were cinnamaldehyde and aniline. From the rate equation, substituent effect and final products, the hydrolysis of cinnamylidene aniline derivatives was initiated by the neutral molecule of $H_2O$ which does not dissociate at below pH 9.0${\sim}$12.0, but proceeded by the hydrogen ion at above pH 5.0${\sim}$9.0.

Optimization of TiCl4 Concentration and Initial pH for Phosphorus Removal in Synthetic Wastewater (합성폐수 내 인을 제거하기 위한 TiCl4 농도 및 초기 pH 최적조건 도출)

  • Shin, So-Yeun;Kim, Jong-Ho;Ahn, Johng-Hwa
    • Journal of Korean Society on Water Environment
    • /
    • v.31 no.6
    • /
    • pp.619-624
    • /
    • 2015
  • This study experimentally determined the effect of titanium tetrachloride (TiCl4) concentration ([TiCl4]) (0.25-0.55 mM) and initial pH (3-11) on phosphorus (P) removal in synthetic wastewater (2 mg P/L). The P removal efficiency increased when [TiCl4] increased. The P removal efficiency showed a parabolic trend with an inflection point at pH 7. At the molar ratio of TiCl4 and P>6.2, the P removal efficiency was over 90% and the residual P concentration was less than 0.2 mg/L. Within the design boundaries, the complete P removal could be achieved at 7.0≤initial pH≤8.5 and 0.43≤[TiCl4]≤0.55 mM. The final pH was over 5.8 at initial pH≥7.7 and [TiCl4]≥0.35 mM. The results showed that TiCl4 was effective in P removal in water so that it could be an alternative chemical for P removal.

Nitrogen and Phosphorus Removal Efficiency of Aluminum Usage in DEPHANOX Process (DEPHANOX 공정 내 알루미늄 첨가에 따른 질소 및 인 제거 효율 평가)

  • Lee, Beom;Park, Noh-Back;Tian, Dong-Jie;Heo, Tae-Young;Jun, Hang-Bae
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.34 no.5
    • /
    • pp.295-303
    • /
    • 2012
  • Removal of total nitrogen (T-N) and total phosphorus (T-P) was evaluated in a DEPHANOX process by adding Al(III) to the separator to maintain T-P in the final effluent below 0.2 mg/L. pH in each reactor was maintained 7~8 after addition of Al(III) to the levels of 5, 10, 15 mg/L. The removal efficiency of COD and T-N decreased at higher Al(III) dose, but T-P removal efficiency increased from 76.28 to 84.02, 94.66% at Al(III) dose of 5, 10, 15 mg/L, respectively. T-P in effluent showed 0.17 mg/L at Al(III) dose of 15 mg/L. Minimum 15 mg/L of Al(III) was required to maitain T-P below 0.2 mg/L in the final effluent.

The Changes of Chlorophylls in Blanched and Fermented Chinese Cabbage (배추의 가열과 산 발효에 따른 Chlorophylls의 변화)

  • 김예숙;이혜수
    • Korean journal of food and cookery science
    • /
    • v.1 no.1
    • /
    • pp.27-32
    • /
    • 1985
  • The changes of chlorophylls and pH of cooked water were investigated in blanched and fermented cabbage. In blanched cabbage, pheophytins were increased markedly with heating time. This was related to the changes of pH and the effects of heat. One minute blanching accelerated the formation of chlorophyllides by the activation of chlorophyllase. In fermented cabbage, pH and chlorophylls were decreased rapidly at the beginning of fermentation. At the final of fermentation, all chlorophylls and chlorophyllides were converted to pheophytins ana pheophorbides, and the content of pheophytins was higher than the content of pheophorbides. The content of pheophorbides in fermented cabbage was higher than that in blanched cabbage.

  • PDF

Screening and Optimal Culture Conditions of Antibiotic-Producing Actinomycetes B-51 for Multidrug Resistant Acinetobacter baumannii (다제내성 Acinetobacter baumannii에 유효한 방선균 B-51의 탐색 및 이 균주가 생산하는 항생물질 발효 최적 배양 조건)

  • Rhee, Moon-Soo;Kim, Gwan-Pil;Bang, Byung-Ho
    • The Korean Journal of Food And Nutrition
    • /
    • v.23 no.1
    • /
    • pp.63-69
    • /
    • 2010
  • With the increase of the use of antibiotics and invasive procedures, infections caused by multidrug-resistant Acinetobacter baumannii(MRAB) are increasing. We screened the antibiotic producing strain B-51 for antibacterial activity against MRAB from the soils and studied the effects of culture medium on the antibiotic production of B-51. The medium conditions for maximum antibiotic productivity of B-51 was 2% glycerol, 0.5% soybean meal, 0.01% $CaCl_2$, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.01% $KH_2PO_4$ at an initial pH of 6.0, at $30^{\circ}C$ for 76 h.

Properties of Alumina Powder Prepared by Precipitation Method (III) : Sintering Behavior (침전법으로 제조한 Alumina 분말의 특성(III) : 소결거동)

  • 홍기곤;이홍림
    • Journal of the Korean Ceramic Society
    • /
    • v.25 no.5
    • /
    • pp.465-472
    • /
    • 1988
  • Alpha alumina powder with fine particle size and narrow particle size distribution was prepared by precipitation method using Al2(SO4)3.18H2O as a starting material. The alpha alumina powder was prepared by calcining aluminum hydroxide which was formed under various pH values. The sinterabilityof alpha alumina powder and the effect of MgO on the sinterability of alpha alumina powder were investigated. The sinterability of alpha alumina powder was the order of pH=10≒pH11>pH=7≒pH9, and alpha alumina obtained from boehmite which was prepared by precipitation method reached to 97.5% of theoretcal density by the pressureless sintering. The effect of MgO on volume shrinkage of alumina was very slight in the initial sintering stage but remarkable in the final sintering stage. It was also found that MgO controlled effectively the grain growth of alumina.

  • PDF

Evaluation of Aluminum and Copper Biosorption in Two-Metal System using Algal Biosorbent

  • Lee, Hak-Sung;Volesky, Bohumil
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
    • /
    • v.2 no.2
    • /
    • pp.149-158
    • /
    • 1998
  • Biomass of non-living brown seaweed Sargassun fluitans pretreated with NaOH is capable of taking up more than $10\%$ $(q_{max}$ : 3.85 mmol/g for Al and 1.48 mmol/g for Cu) of its dry weight in the Al and Cu at pH of 4.5. However, the maximum Al and Cu uptakes calculated from Langmuir isotherm were 1.58 mmol/g for Al and 1.35 mmol/g for Cu at pH 3.5. Equilibrium batch sorption study was performed using two-metal system containing Al and Cu. The mathematical model of the two-metal sorption system enabled quantitative estimation of one-metal biosorption inhibition due to the influence of a second metal. NaOH-treated S. fluitans contained 2.19 mmol $(43\;wt.\%)$ carboxyl groups per gram of biomass. A modified form of Langmuir, which assumes binding of Cu as $Cu^{2+}$ and Al as $Al(OH)_2^+,$ was used to model the experimental data. This result agrees with the one of mono-valent sorption for Al in single-metal system. The modified Langmuir model gives the following affinity correlated coefficients: 0.196 for Cu and 6.820 for Ah at pH 4.5, and 2.904 for Cu and 3.131 for Al at pH 3.5. The interference of Al in Cu biosorptive uptake was assessed by `cutting' the three dimensional uptake isotherm surfaces at constant second-metal final concentrations. Equimolar final equilibrium concentrations of Cu and Al of 1 mM at pH 4.5 give Cu and hi uptakes reduced by $82.5\%\;and\;5.4\%,$ respectively. However, these values at pH 3.5 were $55\%\;(Cu)\;and\;31\%$ (Al).

  • PDF

Changes in Sugar Content and Invertase Activity in Plum Fruits during the Maturation (자두과실(果實)의 성숙중(成熟中) 당함량(糖含量) 및 Invertase의 활성변화(活性變化))

  • Kim, Hyun Ju;Moon, Kwang Deok;Sohn, Tae Hwa
    • Current Research on Agriculture and Life Sciences
    • /
    • v.6
    • /
    • pp.129-135
    • /
    • 1988
  • The changes of sugar content and invertase activity during maturation of plum fruits, and properties of the enzyme were investigated in this study. The soluble sugars in plum fruits were mainly sucrose, glucose and fructose. The sucrose content in the fruit increased slowly at the early stage of maturation and then decreased slightly. At the final stage, the sucrose content increased remarkably with maturation. The contents of glucose and fructose increased slowly at the early stage of maturation following decrement at middle stage. At the final stage, glucose content decreased continuously while fructose content increased again following decrement. Invertase activity in the fruit increased during maturation showing maximum at the onset of color change and after that, decreased remarkably. The optimum pH and temperature of invertase activity were pH 5.0 and $65^{\circ}C$, respectively. The enzyme was most stable at pH 5.0 and retained 75% of its activity after incubation at $70^{\circ}C$ for 15min. The enzyme was activated by $Cu^{{+}{+}}$, $Ca^{{+}{+}}$, but inhibited by $Hg^{{+}{+}}$ remarkably.

  • PDF