• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.105-111
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• 2006

Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase $A_2$ and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.5
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• pp.673-679
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• 2004

Particle breakthrough can occur by either the breakoff of previously captured particles (or flocs) or the direct passage of some influent particles through the filter. Filtration experiments were performed in a laboratory-scale filter using spherical glass beads with a diameter of 0.55 mm as collectors. A single type of particle suspension (Min-U-Sil 5, nearly pure $SiO_2$) and three different destabilization methods (pH control, alum and polymer destabilization) were utilized. The operating conditions were similar to those of standard media filtration practice: a filtration velocity of 5 m/h. To assess the possibility of particle detachment during the normal filtration, a hydraulic shock load (20% increase of flow rate) was applied after 4 hours of normal filtration. The magnitude of particle detachment was proportional to the particle size for non-Brownian particles. At the same time, less favorable particles, i.e., particles with larger surface charge, were easily detached during the hydraulic shock load. Therefore, proper particle destabilization before filtration is crucial for maximum particle removal as well as minimum particle breakthrough.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.3
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• pp.194-201
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• 2003

This study presents the comparative filtration performance evaluation of automotive fuel filters based on the theory of Beta ratio. For the experiments, the fuel component's test stand incorporating the multi-pass filtration test circuit was developed. A mathematical description of filtration process in general was derived. And the theoretical basis of multi-pass test and the test procedure were described in detail. Experimental results revealed that domestic fuel filter tested could not maintain consistent Beta ratio, that is filtration efficiency, although it had the holding capacity of contaminants close to the specification at maximum pressure drop across the filter assembly. The results of experiments and simulations also showed that filtration system model could be refined including desorption ratio to estimate the variable Beta ratio in service life.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.2
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• pp.161-167
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• 2008

Using an experimental method named filtration-permeation, the influence of sedimentation during filtration was studied for the suspensions composed of particles and that of flocs. The average specific cake resistances measured by filtration do not give accurate values because of the sedimentations during filtration, but the permeation data give quite accurate values. The prolonged permeation rates for the cake formed from particulate suspension do not change, but that rate from the floc filtration changes by the sweeping of flocculant. It is proposed that the cake compressibility can be measured with one set of filtration-permeation experiment by step increase of pressure during permeation period. The another experimental method which can shorten experimental time for floc filtration using filtrationpermeation is also proposed.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.27 no.2
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• pp.115-122
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• 2017

Objectives: This study was designed to evaluate the changes in the particle filtration efficiencies of five cloth masks (4 plate types, 1 cup type) with an increasing number of machine washings and the degree of cloth expansion. Methods: NaCl aerosols were generated using an atomizer and passed through cloth masks in a dynamic aerosol chamber. Particle concentrations were measured both before and after for the cloth masks using an optical particle counter (OPC) in the size range of $0.3{\sim}10{\mu}m$. Results: In the original condition, the filtration efficiencies of the five cloth masks were A: 20.1%, B:30.9%, C: 25.0%, D: 26.5%, and E: 40.9%. As the number of washings increased in the order of one, two, and four times, the filtration efficiencies of cloth mask C increased. The filtration efficiency of A, D, and E increased after the first washing. With the exception of B, the filtration efficiency of cloth masks increased after the second washing and those of all cloth masks increased after the fourth washing. This might be caused by the fibers untangling from the yarn and being freed at one end. In this status, the packing density of the textile will not change, but the distances between fibers will increase, which might bring about an increase in filtration efficiency. When the cloth masks were extended by 10% and 20% in one direction, the filtration efficiencies of cloth masks B, D, and E decreased at 10% extension, and those of all cloth masks decreased at 20% extension. When the cloth masks were expanded by 10% and 20% in two directions, the filtration efficiencies of all cloth masks decreased by at least 34.7% at 10% extension, and by at least 60.9% at 20% extension Conclusions: The filtration efficiency of cloth masks could decrease after one to two machine washings, but will increase after four washings in comparison with their original performances. The filtration efficiency of cloth masks will decrease when they are expanded, such as when stretching over the nose during wearing status.

• Korean Chemical Engineering Research
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• v.50 no.2
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• pp.317-327
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• 2012

The average specific cake resistance, the most important indicator for cake filtration and solid-liquid separation, is measured by filtration experiment. But the exact value is difficult to measure because of the other influences such as sedimentation during filtration. This study, a little more stable method named filtration-permeation is proposed for measuring average specific cake resistance. The filtration-permeation is composed of permeation of particle eliminated water through pre-formed cake by filtration. Using 1 wt% calcium carbonate suspension, the filtration-permeation experiments were performed for 8 kinds of filter media at the conditions of 0.5 atm and 0.2 atm, 1 and 3 sheets of filter media. At each specific condition, three to five times filtration-permeation were accomplished. As a result, stable permeation speed is measured. According to this experimental result, the characteristics of permeation and the effect of sedimentation are analyzed with Ruth's equation. The one way analysis of variance (one way ANOVA) is applied to the average specific cake resistances of filtration and permeation obtained with the selected three kinds of filter media. The average specific cake resistances between 0.5 atm and 0.2 atm by filtration do not distinguished, but those by permeation is perfectly distinguished. The experimental results during permeation have a very narrow distribution than that measured during filtration. The analysis of filtration experiments, it was verified that the resistance of filter medium by traditional method is of no significance. Finally, the migration of small particles through the medium composed of fiber glass at low pressure was studied.

• Journal of Korean Society of Water and Wastewater
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• v.23 no.6
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• pp.855-864
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• 2009

The main object of this work was to determine the influence of periodic chemical backwash on filtration resistance in membrane filtration system. In this work Hermia's models were used to investigate the fouling mechanisms involved in the microfiltration of $0.45{\mu}m$ filtered sewage feed. Batch microfiltration experiments were performed at transmembrane pressure 0.4 bar and different feed SCOD concentration (9~67 mgSCOD/L). The results showed that the best fit to experimental data corresponded to the intermediate blocking model followed by the standard and complete blocking model for all the experimental conditions tested. From the simulation results of filtration performance, it was found that in order to maintain sustainable operation of membrane filtration system, irreversible foulant component accumulated continuously on membrane surface and/or pore must be effectively removed. In addition, it was verified that periodic chemical backwash using NaOCl or NaOH effectively improved filtration performance of membrane.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.5
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• pp.666-672
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• 2004

Granular media filtration is used almost universally as the last particle removal process in conventional water treatment plants. Therefore, superb particle removal efficiency is needed during this process to ensure a high quality of drinking water. However, every particle can not be removed during granular media filtration. Besides the pattern of particle attachment is different depending on physicochemical aspects of particles and suspension. Filtration experiments were performed in a laboratory-scale filter using spherical glass beads with a diameter of 0.55 mm as collectors. A single type of particle suspension (Min-U-Sil 5) and pH control was used to destabilize particles. The operating conditions were similar to those of standard media filtration practice: a filtration velocity of 5 m/h. More favorable particles, i.e., particles with smaller surface charge, were well attached to the collectors especially during the early stage of filtration. This selective attachment of the lower charged particles caused the zeta potential distribution (ZPD) of the effluent to move to a more negative range. On the other hand, the ZPD of the effluent did not keep moving from less negative to more negative during the later stages of filtration, and this result was thought to be caused by two reasons: ripening effects and the detachment of flocs.

• Journal of Soil and Groundwater Environment
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• v.16 no.6
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• pp.19-26
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• 2011

In this research, soil analysis and adsorption were conducted, and compared with the water quality of bank filtration and river in terms of TS, TDS, SS, $NH_4$-N, $NO_3$-N, $NO_2$-N, Fe, Mn, BOD and $KMnO_4$ consumption for the development of bank filtration in Gimhae city. Analysis of soil showed high levels of Fe (470 mg/kg), Mn (80 mg/kg) and $NH_4$-N (15 mg/kg). Also, adsorption coefficient values (k and 1/n) were 0.00159 and 0.8714, respectively. This implies that the adsorption of the soil depends on organic matter. Water qualitiy of the river and the bank filtration revealed that the concentrations of TS and TDS almost didn't change but the concentration of SS decreased 84% through the bank filtration. $NH_4$-N in the bank filtration was detected more than 1 mg/L which might be due to agricultural activities in the research area. $NO_3$-N was close to the detection limit owing to the removal by the adsorption. $KMnO_4$ consumption and BOD of the river were decreased by the bank filtration 250% and 350%, respectively, while Fe and Mn were significantly increased by the bank filtration.

• Proceedings of the Membrane Society of Korea Conference
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• 2004.07a
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• pp.57-68
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• 2004

A demonstrative operation of a membrane system with its caparity of 3,600m$^3$/d was carried out using reservoir water as raw water for the application of membrane filtration system to drinking water treatment. The operation was undertaken at a constant flux of 0.9 m$^3$/m$^2$/d for three months. Backwashing with NaClO of 3 ppm was allowed for 30 seconds every 20 minutes of filtration. Physical cleaning was introduced after 69 times of filtration/backwashing cycle with air-scrubbing and backwashing for 1 minute, and flushing for 2 minutes. In this study, water treatment performance was investigated compared with the existing rapid sand filtration process. The membrane system was operated with no significant problems during the test period. Higher water quality was obtained in the membrane filtration than in the rapid sand filtration in terms of particulate matters such as turbidity and microbes. Although the finished water of the membrane filtration contained slightly higher concentration in dissolved matters than that of the conventional one, it met the drinking water standard. The demonstrative operation showed that membrane filtration has a reliability in drinking water treatment. Researches should be needed on cost analysis through long-term operation and optimization of operation condition for further application.