• Korean Journal of Environmental Agriculture
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• v.19 no.1
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• pp.13-19
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• 2000

Seasonal variations of dominant algae species and the effects of these algae on coagulation and filteration of water treatment were investigated at Chilseo water treatment plant in downstream of Nakdong river from January in 1995 to Desember of 1998. The water quality of Nakdong river was found to be a hyper eutrophic state during the investigation periods. In the measurement, Chlorophyll-a contents ranged $20.7{\sim}180.9{\mu}g/l$ and total nitrogen contents(T-N) and total phosphorus contents(T-P) exceeded more than 3.4mg/l and 0.1mg/l, respectively. The changes in dominant algae species was in the order of Stepanodiscus sp., Asterionella sp., Melosira sp., Microcystis sp. and Synedra sp. from spring to winter. Microcystis sp. especially, was blooming during summer and Synedra sp. and Stepanodiscus sp. during winter. Although most diatomous algae appeared in the water treatment process caused filter clogging and reduced efficiency of coagulation and sedimentation, Synedra sp. and Stepanodiscus sp were revealed as the main trouble algae. Malfunction of water treatment process caused by Synedra sp. and Stepanodiscus sp. started at the algae concentrations of 800cells/ml and 1,820cells/ml, respectively. When chlorophyll-a content was $18.9{\mu}g/l$, the optimum amounts of coagulant were found to be 40mg/l of Alum and 16mg/l of PACS. Under condition of chlorophyll-a content of $154.1{\mu}g/l$, addition of Alum at the level of 75mg/l and PACS at the level of 35mg/l showed the lowest turibidity. The result indicates that increased amounts of the coagulants should be added for a better water treatment as chlorophyll-a contents increased. Addition of Alum at the amount of 60mg/l and 30mg/l of PACS removed Stepanodiscus sp. algae at the rate of 85% and 83%, respectively. In case of Synedra sp., 50mg/l of Alum and 25mg/l of PACS showed removal rates of 79% and 81%, respectively. Synedra sp. algae at the standing crops of 1,500cells/ml started filter clogging and a filtering process was completely inhibited after 8 hours. At this situation the filter clogging by Synedra sp. algae occurred at the depth of 5cm from the top anthracite layer. On the other, other algae did filter clogging at the depth of 10cm.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.696-702
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• 2005

Ozonation is a disinfection technique of harmful mi-crobes commonly used in the treatment of drinking water. And Biological Activated Carbon (BAC) treatment also provides numerous benefits for drinking water utilities, including removal of micro- pollutants, improved treatment processes. The multiful-stage ozonation and BAC play roles as effective methods for removing several materials in raw water. Water quality variation in Nak dong river and the removal efficiency of viruses by ozonation-BAC process were investigated on pilot scale. During the period of survey, most of water quality parameters including $NH_{4}^{+}-N$ were highly improved after passing through the BAC. The removal efficiency of poliovirus type III in water treatment process using pilot-plant,$ 99.6\% $ of viruses were removed by pre-ozonation, sedimentation and sand filteration process, $ 100\% $ were removed after in BAC filteration step. In the removal survey of viruses by ozonation, ap-proximately $ 61.1\% $ or polioviruses were inactivated by ozone of 0.4 mg/l within 5 min. and $ 100\% $ were inactivated by ozone of 0.8 mg/l over 10 min.

• KSBB Journal
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• v.14 no.1
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• pp.96-102
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• 1999

Marine bacterium Bacillus cereus ASK202 produced an extracellular agarase (E.C.3.2.1.81) which showed a high level of enzyme activity in the presence of agar and agarose. In the optimal culture conditions, the agarase production increased 7.7 folds compared with the one obtained from the basal medium. Agarase production reached upto 160 units/L after 24hr of cultivation in a modified marine medium at $25^{\circ}C$. The degree of purification increased 31.5 folds with 27.8% yield through freeze drying, DEAE Sepharose CL-6B and Superose 6HR 10/30 column chromatography. The molecular weight of the purified agarase was determined to be 90,000 daltons by gel-permeation filteration. Optimal temperature and pH for the enzyme activity were $40^{\circ}C$ and 7.8, respectively. The enzyme was stable up to $50^{\circ}C$ and at a broad pH range of 5.0-10.0. The $\beta$-agarase was activated by $Zn(NO_3)_2$, and was inhibited by $CuSO_4$ and $SnCl_2$. The Km and Vmax values of this enzyme for agarose as a substrate was $2.4mg/m\ell$ and 13.6 mg/m$\ell$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.2
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• pp.227-236
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• 1995

The objective of this research was to characterize fucoidans isolated from Laminaria religiosa and Undaria pinnatifida in Korea to obtain basic data for Production of soluble dietary fiber materials with biological functionality. Fucoidans were successively extracted 3 times at $65\%$ for 1hr with arid solution of pH 2.0, and cetylpyridinium chloride was used for partial purification. The yields of partially purified fucoidans were $2.71\%$ for L. religiosa, $6.65\%$ for sporophylls of U. pinnatifida and $0.40\%$ for blade of U. pinnatifida. The yield from sporophylls of U. pinnatifida was highest among the sample tested, whereas the yield from blade of U. pinnatifida was lowest. It appeared that the fuconidans content in different parts of U. pinnatifida varied. Partially purified fucoidans were separated into 3 fractions by DEAE-Sephadex A-25 ion exchange column and the maior fractions were refractionated with tractional precipitation with ethanol. $60-70\%$ ethanol precipitated fractions of 1. religiosa and sporophylls of U. pinnatifida turned out to be homogeneous by cellulose acetate electrophoresis and gel filteration chromatography. The molar ratios of fucose, galactose, and sulfate in the purified fucoidans(ethanol precipitated fractions) were 1 : 0.31 : 2.43 for L. religiosa and 1 : 0.97 : 1.99 for sporophylls of U. pinnatifida. The averaged molecular weights of the purified fucoidans from L. religiosa and sporophylls of U. pinnatifida were 31,000 and 38,000, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.5
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• pp.432-442
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• 1992

To identify the histological site of synthesis of yolk protein precursor, vitellogenin, by immunocytochemical method in the freshwater crab Eriocheir japonicus, we purified the yolk protein, vitellin, from crude egg extracts, and prepared the anti-rabbit serum against vitellin. Then, the site of vitellogenin synthesis was demonstrated by immunotytochemical method with PAP(peroxidase-antiperoxidase) reaction using the rabbit antiserum aganist vitellin. Female specific serum protein was identified in female serum by immunoelectrophoresis and Ouchterlony's immunodiffusion test for mature male and female sera. Based on the immunoelectrophoresis and Ouchterlony's diffusion test for mature male and female sera and crude egg extracts using antiserum against vitellogenic female serum absorbed with male serum, the female specific serum protein was identified as vitellogenin, detected in female serum only. The major yolk protein, vitellin, was purified from the crude egg extracts by DEAE-cellulose ion exchange chromatography, followed by sepharose CL-4B gel filteration chromatography. The molecular weight of vitellin was estimated to be about 245,000 dalton by sepharose CL-4B gel filteration chromatography. from the results of immunological analysis for vitellin, it was found that the vitellin antiserum contained the antibody against vitellogenin. In the results of immunocytochemical reaction by PAP method with the rabbit antiserum against vitellin, the vitellogenic oocytes and the hepatopancreas of mature female showed positive PAP reaction, but not in follicle cells and previtellogenic oocytes nf ovary, muscle of female and mature male hepatopancreas. Therefore, it showed that the hepatopancreas of mature female is the site of vitellogenic synthesis.

• Journal of Korean Society of Water and Wastewater
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• v.9 no.2
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• pp.97-107
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• 1995

Today a conventional water treatment system has many problems. The ozone/GAC process, sometimes termed Biological Activated Carbon(BAC), appeared to be effective for the removal of soluble organic matters in the drinking water. The water quality of Nak-dong river in Pusan, generally shows BDOC 30-40% and NBDOC 60-70%. The pilot plant installed at the Duk-san water works that was been largest treatability(1,650,000ton/day) in Pusan. A experimental water in the pilot plant made use of the water after sand-filteration. Following results are drawn from this study. Initial adsorption velocity($DOC/DOC_o/T$) in the pure adsorption of GAG had a 0.0225, it's velocity changed to 0.006 after ozone added and the optimum ozone dose ranged of $1.4-2.0mgO_3/L$. A experimental water in the pilot plant composed with humic material(78%). Humic material composed with humic acid(20%) and fulvic acid(56%), and it's rate changed to 18 and 50% respectively after ozone added. DOC constantly decreased in the EBCTs and removal efficieny in the 15min of EBCT was 45-50%. It showed the largest removal rate of BDOC in the EBCT 5 and among the season, characteristics of removal varied. The HPC distributed over $10^6-10^7CFU/cm^3$ in the bed depth and among the season, distribution of HPC were differential.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.435-441
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• 2010

We report the preparation and characterization of a new and water soluble complex of palladium(II) with 1,10- phenanthroline and butyldithiocarbamate ligands. This compound has been studied through spectroscopic techniques, $^1H$ NMR, IR, electronic spectra and elemental analysis and conductivity measurements. The complex shows 50% cytotoxic concentration ($Ic_{50}$) value against chronic myelogenous leukemia cell line, K562, much lower than that of cisplatin. Thus the mode of binding of this complex to calf thymus DNA have been extensively investigated by isothermal titration UV-visible spectrophotometry, fluorescence, gel filteration and other methods. UV-visible studies show that the complex exhibits cooperative binding with DNA and remarkably denatures the DNA at extremely low concentration ($~13\;{\mu}M$). Fluorescence studies indicate that the complex intercalate into DNA. Gel filtration studies suggest that the binding of Pd(II) complex with DNA is strong enough that it does not readily break. In these interaction studies, several thermodynamic and binding parameters are also determined which may reflect the mechanism of action of this type of compound with DNA.

• Journal of Environmental Science International
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• v.15 no.2
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• pp.133-139
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• 2006

Soil biofiltration is an environmentally-sound technology for elimination of VOCs, odorous and NOx compounds from a low concentration, high volume waste gas streams because of its simplicity and cost-effectiveness. This study was performed to evaluate effect of removal of gaseous NOx using a soil and a yellow soil. Over $60\%\;and\;48\%$ of NOx from a soil and a yellow soil was removed at the inlet NO concentrations of $423\~451$ppb, respectively. The bio-filter using a soil media was capable of purifying NOx with a different natural processes. Although some of the processes are quite complex, they can broadly be summarized as adsorption into soil pore water, and biochemical transformations by soil bacteria. When the filteration bio-reactor was applied to a soil and a yellow soil, effective NOx removal was obtained for several times and months. These results show that a soil biofilter can be of use as an alternative advanced NOx treatment system.

• The Korean Journal of Ecology
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• v.26 no.5
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• pp.263-266
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• 2003

This research investigated the process of removing cadmium and tested the detoxification mechanism of the cadmium-binding peptide (Cd-BP) from Rumex crispus. Phytochelatin-like cadmium-binding peptide (PC-Cd-BP) of Rumex crispus was purified and identified. Rumex crispus was exposed to 4.3 mg Cd/L for seven days. Heat-treated supernatant fraction taken by root tissues showed traces of PC-Cd-BP An analysis of the material through Gel-filteration chromatography on the Sephadex G-75 column showed two symmetrical Cd-BP peaks. The major peak with the smaller molecular weight was further purified by $C_{18}$ reverse-phase HPLC to produce apparent homogeneity. The amino acid composition of Cd-BP from Rumex crispus included cysteine (22.6%), glutamate and glutamate acid (20%), and glycine (12%). It was similar the amino acid composition of most PC. The molecular weight of the purified peptide was determined at 568-706 Da by MALDI-TOF MS. Therefore, the Cd-BP of Rumex crispus was PC-Cd-BP consisting of isopeptides.

• Textile Coloration and Finishing
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• v.16 no.4
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• pp.10-18
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• 2004

In this work, Aqueous sericin solution was prepared by degumming process with electrolytic reduction water. Then, the microfiltration and ultrafiltration systems were applied to the concentration of aqueous sericin solution. The objective of this study was to select the optimum operating condition among the different pressure. The permeate flux and rejection ratio were observed with time, pressure, flow rate and concentration. and, the wastewater and permeated water quality values such as pH, BOD, COD, and NH levels were measured. In order to see the influence of electrolytic reduction water, the flux of pure water and electrolytic reduction water by PVDF22(MF) and PS100(UF) membrane was measured. In microfiltration system, the relative flux reduction decreased rapidly to 0.02 in the 30min, as the concentration polarization and gel layer formation were increased. and then the sericin concentration rejection ratio was 40%. In ultrafiltration system, the permeate flux decreased with time and concentration, and increased with the operating pressure and flow rate. Optimal condition in PS100 membrane system for sericin concentration was operating pressure 1.464kgf/$cm^24, operating flow rate $7\ell/min at\; 40^{\circ}C$. At that time, sericin concentration rejection ratio was 83% respectably. The sericin solution was concentrated from 0.1wt% solution to 0.2 wt % solution during about 2 hrs by the UF filteration membrane system.