• 제목/요약/키워드: field-collected strain

검색결과 70건 처리시간 0.023초

광합성세균에 의한 수소 생산에 관한 연구 (제 1보) 광합성균의 분리 및 동정 (Studies on Hydrogen Evolution by Photosynthetic Bacteria(Part 1) Isolation and Identification of the Photosynthetic Bacteria)

  • 배무;양성우;고영희
    • 한국미생물·생명공학회지
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    • 제10권1호
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    • pp.27-32
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    • 1982
  • 광합성세균에 의한 수소산을 목적으로 성남근교의 논 토양으로부터 수소를 다량 생산하는 비유황세균 1주를 분리하고 동정하였다. 이 균주의 생리적 형태적 및 배양상의 특성을 규명한 결과 Rhodopseudomonas gelatinosa K-13으로 동정되었다.

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아스팔트 鋪裝道路의 確率論的 表層設計 (Pobabilistic Design of Asphalt Pavement Surface Courae)

  • 김광우;연규석
    • 한국농공학회지
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    • 제34권1호
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    • pp.66-77
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    • 1992
  • A prototype probabilistic approach to thickness design for asphalt pavement surface course was developed using first-order second moment probability model. The tensile strain (load effect) developing at the bottom of surface layer due to the wheel load and the critical strain (resistance) of asphalt concrete were used as random variables for pavement reliability analysis. Based on the parameters for load effect and resistance data collected from reference and field, simulated data were generated by Monte Carlo method for reliability evaluation of the pavement for a typical rural highway. Thickness of pavement surface course was defined in terms of target reliability of the pavement, growth factor of traffic, design life of pavement and resistance of the asphalt concrete to be placed on the pavement. According to these rationales, prototype thickness design chrats were sugested through example studies. From these, similar design charts can be developed for many pavements if appropriate data and target reliability are determined.

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FRP Box와 판으로 보강된 교량 바닥판 콘크리트의 휨거동 (Flexural Behavior of Bridge Deck Concrete Reinforced with FRP Box and Plate)

  • 남정훈;정상균;윤순종;김병석;조근희
    • 한국복합재료학회:학술대회논문집
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    • 한국복합재료학회 2004년도 춘계학술발표대회 논문집
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    • pp.13-17
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    • 2004
  • In recent years, the deterioration of reinforced concrete structures has become a serious problem in civil engineering fields. This situation is mainly due to corrosion of steel reinforcing bars embedded in concrete. Recently, there has been a greatly increased demand for the use of FRP (fiber reinforced plastic) in civil engineering field due to their superior mechanical and physical properties. This paper presents an experimental study on the behavior of concrete bridge deck reinforced with FRP Box, FRP Plate, and FRP Re-bar. In tlIe study, mechanical properties of FRP Box, FRP Plate, GFRP Re-bar, and CFRP Grid have been investigated. Full scale one-way deck slab was tested under four point lateral load (equivalent to actual wheel load of DB-24 including impact). Load-deflection and load-strain data were collected through LVDT's and strain gages attached to the specimen.

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복숭아혹진딧물 야외개체군의 살충제 저항성 마커 선발 (Selection of Insecticide Resistance Markers in Field-collected Populations of Myzus persicae)

  • 김주일;권민;심재동;김점순;이영규;지삼녀;이정태;류종수;유동림;이계준
    • 한국응용곤충학회지
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    • 제53권2호
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    • pp.149-156
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    • 2014
  • 2009~2011년 동안 국내 주요 배추 재배지 5개 지역(평창, 홍천, 봉화, 무주, 제주)에서 살충제에 대한 복숭아혹진딧물의 저항성 발달 정도를 조사하고, 야외 개체군에 적용 가능한 살충제 저항성 마커를 개발하기 위해 본 연구를 수행하였다. 조사된 5개 지역 개체군 모두 여러 살충제 종류에 대하여 다양한 저항성을 보였다. 다양한 저항성을 보인 5개 지역 야외 개체군으로부터 여라 살충제에 대하여 복합적으로 저항성을 보이는 복합저항성 계통(MR)을 선발하였고, 이 MR과 모든 지역 채집 개체군에 대해 등전점전기영동과 정량염기서열분석(quantitative sequencing, QS)을 통하여 에스터레이즈 과발현과 살충제 작용점 내 돌연변이를 확인하였다. MR을 포함한 모든 야외 개체군에서 에스터레이즈의 과발현과 아세틸콜린에스터레이즈 1 유전자(ace1)의 StoF 돌연변이를 확인할 수 있었다. 넉다운 저항성 돌연변이로 잘 알려진 파라 타입 나트륨 채널 유전자(para)의 LtoF 돌연변이는 모든 지역 채집 개체군은 물론 비펜스린에 대해 3,461배 저항성을 보이는 MR에서도 발견되지 않았다. 그 외에 MtoL 돌연변이를 발견하였는데, 생물검정 결과 저항성 수준과 돌연변이 발생 빈도가 일치하였다. 따라서 생물검정 대신, 이러한 분자 마커를 활용 한다면 더 효율적으로 살충제 저항성 평가가 가능할 것이다. 이러한 분자 마커들(ace1의 StoF, para의 MtoL)은 정량염기서열분석, PCR amplification of specific alleles (PASA) 등의 진단 방법에 쉽게 응용이 가능 하고, 이러한 방법은 야외 복숭아혹진딧물 저항성 관리에 적용이 가능 할 것이다.

응집제 Ceramic-Zeolite type과 황토를 이용한 Microcystis aeruginosa 제거에 관한 연구 (A Study on the Removal of Microcystis aeruginosa by Coagulants of the Ceramic-Zeolite type and Yellow loess)

  • 박홍기;정은영;이유정;정종문;최식영;홍용기
    • 한국환경과학회지
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    • 제11권4호
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    • pp.405-409
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    • 2002
  • For the proposal of Microcystis aeruginosa control technique by coagulants, removal effects of coagulants were carried out using isolated strain and collected water bloom of M. aeruginosa on Downstream of the Nakdong River. Both of purified and field-collected M. aeruginosa were entirely sedimented by the addition of the coagulant ceramic-zeolite type Co 100 (1.5 mg/$\ell$) within 24 hr, but Yellow loess (10 mg/$\ell$) was less effective for the removal of M. aeruginosa within 24hr. Thus it was concluded that Ceramic-Zeolite type Co 100 was the most effective coagulant.

점박이응애 야외개체군의 살비제 저항성 모니터링 (Monitoring of Acaricide Resistance in Field-Collected Populations of Tetranychus urticae (Acari: Tetranychidae) in Korea)

  • Jum Bae Cho;Young Joon Kim;Young Joon Ahn;Jai Ki Yoo;Jeong Oon Lee
    • 한국응용곤충학회지
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    • 제34권1호
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    • pp.40-45
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    • 1995
  • 전국 8개 지역별 각 사과원에서 채집된 점박이응애(Tetranychus urticae Koch)에 대한 저항성 정도를 일본 감수성 계통과 비교한 결과 지역별 현저한 감수성 차이를 보였다. Azocyclotin, fenpropathrin, propargite 및 abamectin에 대해서는 낮거나 중간 정도의 저항성을, dicofol, fenpyroximate 및 pyridaben에 대해서는 높은 저항성을 나타내었다. 이들 계통은 한종 또는 두종 이상의 약제에 대해 감수성을 보여 특정 지역에 대해서는 적당한 살비제의 선택적 이용으로 점박이응애를 효과적으로 방제할 수 있을 것으로 사료된다.

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Corbicula fluminea (Bivalvia: Corbiculidae): a possible second molluscan intermediate host of Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea

  • Chung, Pyung-Rim;Jung, Young-Hun;Park, Yun-Kyu;Hwang, Myung-Gi;Soh, Chin-Tack
    • Parasites, Hosts and Diseases
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    • 제39권4호
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    • pp.329-332
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    • 2001
  • More than 1,500 clams of Corbicula fluminea, the most favorable food source of freshwater bivalves in Korea, were collected from 5 localities to examine cercarial and metacercarial infection with Echinostoma cinetorchis. Although 3 clams infected with suspicious E. cinetorchis metacercariae out of 200 specimens collected at Kangjin, Chollanam-do were detected, no cercarial and metacercarial infections with E. cinetorchis were observed in field-collected Corbicula specimens. In the susceptibility experiments with laboratory-reared clams, those infected with miracidia of E. cinetorchis did not release their cercariae up to 60 days after infection. To confirm the identity of second intermediate host of E. cinetorchis experimentally, a total of 30 clams were exposed to the cercariae from Segmentina hemisphaerula that had been infected with miracidia of E. cinetorchis. The clams were susceptible to cercariae of E. cinetorchis with an infection rate of 93.3%. Metacercariae from clams taken more than 7 days after cercarial exposure were fed to rats (S/D strain), and adult worms of E. cinetorchis, characterized by 37-38 collar spines on the head crown, were recovered from the ileocecal regions. This is the first report of C. fluminea as a possible second intermediate host of E. cinetorchis.

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거대억새 단지 조성에 따른 토양 특성 변화 (Change in Soil Properties after Planting Giant Miscanthus)

  • 강구;홍성구;박성직
    • 한국농공학회논문집
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    • 제55권6호
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    • pp.69-75
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    • 2013
  • Miscanthus has received wide attention as an option for biomass production in Korea, recently. New strain of giant miscanthus has been developed and was planted in two large trial sites (184 ha) in the lower reaches of the Geum River. To evaluate the susceptibility of the giant miscanthus as an bioenergy crop for the future, we investigated the influence of the giant miscanthus on the soil properties. The particle size, CEC, pH, EC, T-N, T-P, heavy metal total concentration, and heavy metal fractions of soil samples collected from abandoned field, 1 year old giant miscanthus field (1st Year GM), and 2 year old giant miscanthus field (2nd Year GM) at different depths of 0~15, 15~30, and 30~45 cm in April and August were analyzed. Results showed that the CEC and pH of the soil of the giant miscanthus field were lower than those of the soil of abandoned field. The EC of 2nd GM was lower than that of abandoned field, indicating that the giant miscanthus may facilitate soil desalination process. The organic and sulfide fraction and residual fraction of heavy metals in the giant miscanthus field was higher than in abandoned field, due to the low pH of the giant miscanthus field and the excretion of phytosiderophores by rhizome of the giant miscanthus. This study showed that the giant miscanthus can influence on the soil properties and further study for long term is needed to elucidate the interaction between the soil and the giant miscanthus.

Prevalence and Characterization of Methicillin-Resistant Staphylococcus aureus in Raw Meat in Korea

  • Lim, Suk-Kyung;Nam, Hyang-Mi;Park, Hyun-Jung;Lee, Hee-Soo;Choi, Min-Jung;Jung, Suk-Chan;Lee, Ji-Yeon;Kim, Young-Cho;Song, Si-Wook;Wee, Sung-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제20권4호
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    • pp.775-778
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    • 2010
  • A total of 2,858 meat samples collected during 2003-2008 in Korea were investigated, and methicillin-resistant Staphylococcus aureus (MRSA) isolates were isolated from 1.0% (9/890) of beef, 0.3% (4/1,055) of pork, and 0.3% (3/913) of chicken meat samples, respectively. MRSA isolates showed the two sequence types (STs), ST72 from beef and pork and ST692 from chicken meat. MRSA isolates from beef and pork were Panton-Valentine leukocidin-negative, staphylococcal cassette chromosome mec type IVa strain with ST72, which is the most prevalent type of communityacquired MRSA in Korea. An identical pulse-field gel electrophoresis pattern was detected among 10 of 16 MRSA isolates: 9 strains from beef (n=5) and pork (n=4) in 2008, and one strain from beef in 2005.

시료의 이동과 보관이 시료 교란에 미치는 영향에 관한 연구 (A Study on the Effect of Transportation and Storage on Sample Disturbance)

  • 김병일;이승현;유완규
    • 한국산학기술학회논문지
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    • 제8권5호
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    • pp.1159-1165
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    • 2007
  • 이 연구에서는 시료의 이동과 보관이 시료교란에 미치는 영향을 파악하기 위하여 전라남도 광양시 OO지역에서 채취한 불교란 시료에 대해 현장과 서울 실험실, 그리고 서울 실험실에서 4주간 보관한 후 일축압축, 압밀시험을 수행하였다. 시험결과 얻은 파괴변형률$(\epsilon_f)$, 체적변형률$(\epsilon_{\nu})$, 간극비 변화량$({\Delta}e/e_0)$를 이용하여 시료교란도 평가를 수행하였고, 평가된 시료교란등급을 이용하여 이동과 보관에 대한 시료의 교란정도를 비교, 분석하였다. 연구결과 시료 이동과 보관이 시료교란에 영향을 미치는 것으로 나타났다.

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