• Laboraroty Animal Research
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• 제34권4호
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• pp.160-165
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• 2018

Breast cancer remains the second leading cause of cancer death among woman, worldwide, despite advances in identifying novel targeted therapies and the development of treating strategies. Classification of clinical subtypes (ER+, PR+, HER2+, and TNBC (Triple-negative)) increases the complexity of breast cancers, which thus necessitates further investigation. Mouse models used in breast cancer research provide an essential approach to examine the mechanisms and genetic pathway in cancer progression and metastasis and to develop and evaluate clinical therapeutics. In this review, we summarize tumor transplantation models and genetically engineered mouse models (GEMMs) of breast cancer and their applications in the field of human breast cancer research and anti-cancer drug development. These models may help to improve the knowledge of underlying mechanisms and genetic pathways, as well as creating approaches for modeling clinical tumor subtypes, and developing innovative cancer therapy.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 공동 춘계학술대회
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• pp.68.2-68
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• 1997

No Abstract, See Full Text

• 한국환경과학회지
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• 제9권3호
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• pp.201-208
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• 2000

PCBs and DDE were determined in the soil samples and the Korean striped field mice, Apodemus agrarius, collected at six localities in Changwon and one locality in Gaduck Island from August 1997 to May 1998. The residual level of PCBs in soils, in which the concentrations of high chlorinated compounds were much higher than those of low chlorinated ones, was highest in the industrial area(IA) in Changwon(60.7 ng/g in average), whereas those of DDE in soils was highest in Gaduck Island(331.9 ng/g in average). However, the levels of each compound detected from soils in the respective remaining places were relatively low. The similar tendency as in the soils was observed in the mice, indicating that the concentration of pollutants in the mice could denote the status of pollution in the terrestrial environment. The composition of PCB congeners in the mice, however, was different from that of soils, but similar to those of other mammals. Moreover, the residual levels of PCBs and DDE in pregnant females from which fetuses were removed were lower than those of males and non-pregnant females. It suggests that these compounds accumulated in pregnant females might have been transferred to the fetuses through placenta as in other mammals. Judging from the above facts, it is suggested that the striped field mouse can be an useful biological index to evaluate the contamination in mammals inhabiting terrestrial environment.

• 한국산학기술학회논문지
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• 제6권2호
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• pp.183-188
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• 2005

포유동물에서는 두가지의 receptor가 보고되었다. 각 sample을 RNA extraction, RT-PCR, Realtime-PCR을 실시하여 melatonin 1A, 1B의 발현 양을 분석하였다. MT1A는 cerebellum에서는 3 hours에서 약 1/8배로 감소를 보이고 6 hours에서는 정상치 9 hours에서는 16배정도로 많은 양의 증가율 보였다. 나머지 hippocampus, thalamus, hypothalamus에서는 공통적으로 3 hours에서 많게는 10배에서, 적게는 대조군과 거의 비슷한 1.5배정도의 증가율을 보이고 있으며, 6 hours에서는 모두 감소하는 것을 알 수 있다. MT1B에서는 4 group 모두 3 hours, 6 hours에서 receptor의 양이 확연히 줄어들었다. 9 hours의 경우에는 4 group모두에서 적게는 8배, 많게는 거의 1000배 가까이의 발현 양의 차이가 나타났다.

• 전자공학회논문지SC
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• 제46권4호
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• pp.70-76
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• 2009

본 연구에서는 자동차 사고나 뇌졸중 둥에 의해 경추 이하의 마비나 손, 발 등의 움직임이 자유롭지 못한 사람들의 컴퓨터 사용을 돕고자 손이나 발을 이용하지 않고 머리의 움직임과 눈의 깜박임만으로 컴퓨터 마우스 제어가 가능한 장치를 제안하였다. 마우스의 위치는 각속도 센서를 이용하여 머리의 움직임으로 추정하고, 눈 깜빡임에 의한 클릭과 더블 클릭은 광센서의 시야를 방해하지 않는 위치에 장착하여 커 클위치와 이벤트를 검출하였다. 제안한 마우스의 공간 이동 능력과 이벤트 검출을 비교한 실험에서는 좌우, 상하 이동은 기존 마우스와 비교하여 속도 면에서는 큰 차이는 없었으나, 정확도가 조금 떨어지는 이유로 인하여 정확한 위치로 이동시키는데 소요시간이 3$\sim$4배 정도 더 필요하였다. 데드 존을 갖는 비선형 상대 좌표계 방식을 이용하여 주기적으로 적분 에러를 제거해야 하는 문제를 해결하였고, 이동 거리와 속도를 함께 고려하여 직관적인 마우스 포인터 제어가 가능하도록 하였다. 주변광의 영향을 최소화하도록 광원 제어 회로를 설계하여 외부 광원의 변화에도 마우스 이벤트 검출이 영향을 받지 않았다.

• Journal of mucopolysaccharidosis and rare diseases
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• 제4권1호
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• pp.26-36
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• 2018

Mucopolysaccharidosis IIIA is a heritable neurodegenerative disorder resulting from the dysfunction of the lysosomal hydrolase sulphamidase. This leads to the primary accumulation of the complex carbohydrate heparan sulphate in a wide range of tissues and CNS degeneration. Characterization of animal model is the beginning point of the therapeutic clinical trial. Mouse model has a limitation in that it is not a human and does not have all of the disease phenotypes. Therefore, delineate of the phenotypic characteristics of MPS IIIA mouse model prerequisite for the enzyme replace treatment for the diseases. We designed 6-month duration of phenotypic characterization of MPS IIIA mouse biochemically, behaviorally and histologically. We compared height and weight of MPS IIIA mouse with wild type from 4 weeks to 6 months in both male and female. At 6 months, we measured GAG storage in urine kidney, heart, liver, lung and spleen. The brain GAG storage is presented with Alcian blue staining, immunohistochemistry, and electron-microscopy. The neurologic phenotype is evaluated by brain MRI and behavioral study including open field test, fear conditioning, T-maze test and Y-maze test. Especially behavioral tests were done serially at 4month and 6month. This study will show the result of the MPS IIIA mouse model phenotypic characterization. The MPS IIIA mouse provides an excellent model for evaluating pathogenic mechanisms of disease and for testing treatment strategies, including enzyme or cell replacement and gene therapy.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2009년도 학술대회
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• pp.701-706
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• 2009

본 연구에서는 자동차 사고나 뇌졸중 등에 의해 경추 이하의 마비나 손, 발 등의 움직임은 자유롭지 않은 사람들의 컴퓨터 사용을 돕고자 손이나 발을 이용하지 않고 머리의 움직임과 눈의 깜박임만으로 컴퓨터 마우스 제어가 가능한 장치를 제안하였다. 마우스의 좌우, 상하 이동은 자이로 센서를 이용하여 머리의 움직임으로 유발하고, 클릭과 더블 클릭은 광 센서를 시야를 방해하지 않는 위치에 장착하여 감지할 수 있도록 하였다. 머리의 움직임에 의한 마우스 이동은 절대 좌표계와 상대 좌표계를 이용하는 두 가지 방법으로 실험하였다. 제안한 장치의 클릭과 더블 클릭의 인식율은 평균 86%에 편차는 5%정도다, 반응속도는 기존 마우스의 이동 속도와 비교해 볼 때 큰 차이는 없었다. 마지막으로 종합적인 평가를 위해 마우스 이동과 클릭을 동시에 수행하였을 경우에는 정확도가 80%에 편차는 4%정도로 기존 마우스보다는 낮았다. 특히 상대 좌표계를 이용하는 경우 절대 좌표를 이용하는 경우보다 좌표의 누적 에러로 인한 reset 빈도를 줄일 수 있었고, 정밀한 마우스 포인터 조작에 편리하였다.

• 한국컴퓨터정보학회논문지
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• 제20권1호
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• pp.153-161
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• 2015

본 논문에서는 연속 촬영 이미지를 이용한 실험용 쥐의 운동 능력 평가 시스탬을 제안한다. 의학이나 수의학 분야에서는 인류의 양질의 삶을 위해 mouse를 이용한 동물실험을 실시하고 있다. 특히 실험용 쥐(mouse)를 이용한 실험 중에는 인위적인 부상(injury)을 만들고, 다양한 처치를 통하여 그 부상에서 회복되는 정도를 측정하는 실험을 많이 하고 있다. 이러한 실험에서 그 동안의 전통적인 운동량 측정 방법은 사람이 직접 관찰하는 방법으로 이루어지고 있었다. 제안한 시스템은 실험용 쥐에 대한 움직임을 정해진 단위 시간동안 연속 촬영을 실시하고, 이 연속 촬영된 이미지에서 mouse의 외곽선을 point cloud로 추출한다. 추출된 point cloud를 이용하여 mouse의 몸통에 대한 내부 외곽선(interior contour line)을 다시 추출한 후, 이를 이용하여 그 중심점을 구하고, 이들 중심점들을 연결하여 거리를 계산하여 누적함으로써 mouse가 단위 시간동안 이동한 총 거리를 자동 계산하도록 하는 시스템을 설계하고 구현한다.

• Animal cells and systems
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• 제1권2호
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• pp.371-379
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• 1997

To investigate the movement of sperm head and the role of sperm neck in forward sperm motility in the Korean striped field mouse, Apodemus agrarius coreae, the morphological characteristics of the cauda epididymal spermatozoa were examined by light microscopy and scanning and transmission electron microscopy. Spermatozoa of A. agrarius coreae were characterized by the conspicuous shape of the acrosome and the long tail compared with those of other rodents. Total length of the sperm was $133\mu{m}$. The sperm head had a curved falciform shape. The head was 8.0${\mu}$m in length, and about 4.0 ${\mu}$m in width. The shape of acrosome had an openerlike form. The sperm tail (125 ${\mu}$m) consisted of four major segments: neck (0.5 ${\mu}$m), middle piece (29.5 ${\mu}$m), and principal piece plus the end piece (95 ${\mu}$m). The outer dense fibers were arranged in a horseshoe fashion, and No. 1, 5, 6, and 9 of the outer dense fibers were larger than the others. The mitochondrial bundles of middle piece were composed of a pair of arms, which surrounded the axone of the middle piece by the 45 0 angled helical structure. The total number of mitochondrial gyres was 188. In particular, the microfilament structures existed in plasma membrane of the sperm, which was adjacent to the acrosomal region on the nuclear membrane. The segmented columns were surrounded by microfilament structures, and the microfilament bundles were adjacent to the outer membrane of the first mitochondria of middle piece. This study presents for the first time the existence of microfilament structures within the plasma membrane of sperm which is located from the adjacent acrosome region to the connecting piece in sperm neck of Korean striped field mouse, Apodemus agrarius coreae. The present result suggests that the constriction and extension of microfilament in sperm neck as well as the wave-movement of sperm tail may play a role in the movement of sperm head.

• Parasites, Hosts and Diseases
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• 제52권4호
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• pp.419-423
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• 2014

The present study was performed to know the infection status of intestinal helminths in a most common species of field mice, Apodemus agrarius, from 2 southern regions of Korea. Total 133 and 103 mice were collected by the mouse trap in Hapcheon-gun, Gyeongsangnam-do and Gurye-gun, Jeollanam-do, respectively, from July 2005 to June 2006. The small intestine of each mouse was resected and longitudinally opened with a pair of scissors. The intestinal contents were washed with 0.85% saline until the supernatant became clear. Helminths were collected with naked eyes or under a stereomicroscope from the sediment of the intestinal content. More than 11 species of helminths (4 nematode spp., 5 trematode spp., and 2 cestode spp.) were recovered. Among these, heligmosomoid nematodes (97.5%) was the most highly and heavily infected species. As the members of trematodes, Plagiorchis muris, Brachylaima sp., Echinostoma hortense, Echinostoma cinetorchis, and unidentified echinostome larvae were found in the small intestines of 35 (14.8%), 12 (5.1%), 6 (2.5%), 1 (0.4%), and 1 (0.4%) mice respectively. Two species of tapeworms, Hymenolepis nana and Hymenolepis diminuta were also detected in 79 (33.5%) and 21 (8.9%) mice, respectively. Conclusively, heligmosomoid nematodes were the most prevalent (dominant) species among more than 11 helminth species detected, and Brachylaima sp. fluke is newly added in the list of intestinal trematodes in Korea.