• 제목/요약/키워드: fibrin-binding domain

검색결과 1건 처리시간 0.014초

피브린 결합능을 가진 합성 펩타이드의 치주인대 세포에 대한 생물학적 영향 (The biologic effect of fibrin-binding synthetic oligopeptide on periodontal ligament cells)

  • 이주아;구영;박윤정;구기태;김태일;설양조;이용무;류인철;정종평
    • Journal of Periodontal and Implant Science
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    • 제39권1호
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    • pp.45-52
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    • 2009
  • Purpose: Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells. Materials and methods: PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed. Results: PDL cells on synthetic oligopeptide coated dishes showed enhanced cell adhesion and proliferation. Western blot analysis revealed increased level of ERK1/2 phosphorylation in cells plated on FN fragment containing fibrin-binding domain(FF1 and FF5) coated dishes. Conclusion: These results reveals that FN fragment containing fibrin-binding domain possess an enhanced biologic effect of PDL ligament cells.