• Journal of Embryo Transfer
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• v.11 no.3
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• pp.225-231
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• 1996

This study was conducted to investigate the effects of preincubation time, concentration and exposure time of sperm on in vitro fertilization of porcine follicular oocytes rnatured in in vitro. The results obtained are as follows ; 1. Effect of preincuhation time for porcine sperm capacitation on in vitro fertilization in medium with heparin was investigated. Normal fertilization rate was highest in 15 min(26.4%). However, there were no significant differences among preincuhation times of 5~90 min, 2. Normal fertilization rates of sperm concentrations were 17.0~26.5%, and normal fertilization rate from l$\times$ l05cell /ml concentration was also higher than those of other sperm concentration. 3. Normal fertilization rates of sperm exposure time of 4, 8, 12, 16 and 20 hours were 6.1, 20.8, 27.8, 25.0 and 26.7%, respectively. Normal fertilization rate from sperm exposure time of 12 hours was also higher than that of other sperm exposure times.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.123-127
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• 1995

In vitro embryo production (IVP) is affected by various factors during in vitro maturation, fertilization, and development. In this experiment, the effect of ovary type, quality of follicular oocyte, medium used for fertilization, presence of hormone in medium, sperm concentration on in vitro maturation and fertilization were examined for effective IVP. In vitro maturation was carried out using TCM-199 supplemented with 15% FCS and hormones in 5% $CO_2$ incubator for 24h. In vitro fertilization was performed with frozen-thawed sperm in modified mTALP medium containing 0.3% BSA, $10{\mu}g/ml$ heparin, and 5mM/ml caffeine for 24h. The fertilized embryos were co-cultured on monolayer of cumulus cells in TCM-199. When oocytes were collected from functionally active and inactive ovaries, maturation rate was 76.9 and 7.7%, respectively. When oocytes were classified morphologically to good and poor grades, maturation rate was 75 and 58.8%, respectively. FSH + LH + $E_2$ (86.4%) showed higher maturation rate than control (53.0%) and FSH (73%). The fertilization rate was 28.2, 100 and 91.7% in $1.6{\times}10^5$, $5.0{\times}10^5$ and $10.0{\times}10^5$ sperm concentration per ml. When oocytes were fertilized in mTALP and BO media, fertilization and cleavage rates of oocytes in mTALP were higher (84.3 and 56.9%) than those (67.4 and 23.3%) in BO medium. In this experiment, in vitro maturation, fertilization and development of oocytes were affected by type of ovary, grade of oocyte, hormones, sperm concentration and fertilization medium.

• KCID journal
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• v.14 no.1
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• pp.57-66
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• 2007

Temporal changes in total nitrogen (T-N) and phosphorus (T-P) concentrations in paddy floodwater in response to fertilization under rain-shielding pot and small-scaled field conditions were investigated. On the basis of the changing patterns, suggestions for the use of fertilization factors, such as days after fertilization, in developing models for the estimation of T-N and T-P loads from paddy fields were made. Total N concentration was susceptible to fertilization, showing a peak concentration right after fertilization followed by a decreasing pattern with the elapse of days after fertilization. The decreasing pattern of T-N concentration followed the first- order kinetics, indicating that the models are likely to be an exponential equation using days after fertilization as an independent variable. Comparison between the pot and field experiments conducted with soils different in soil fertility revealed that indigenous soil N concentration significantly affected T-N concentration, and this suggests that soil N status can be used as the second variable for the models. Meanwhile, temporal changes in T-P concentration did not respond to P fertilization as sensitively as T-N. In combination with other published results, our study suggests that rainfall intensity and other factors associated with farming activities that are likely to cause disturbance of soil particles containing P may be used as possible variables for the models.

• Korean Journal of Animal Reproduction
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• v.22 no.3
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• pp.213-219
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• 1998

This study was carried out to investigate on the improvement of fertilizing ability of in vitro matured oocytes from sperm density, motility and polyvinylpyrrolidine (PVP) concentration, by intracytoplasmic sperm injection(ICSI) into the bovine oocytes. 1. The in vitro fertilization and cleavage rates of oocytes from 1.0, 2.0, 3.0, 5.0 ($\times$106/ml) sperm concentration by IVF and ICSI of bovine oocytes were 45.0%~65.0%, 65.0%~90.0% and 10.0%~30.0%, 35.0%~70.0%, respectively. 2. The in vitro fertilization and cleavage rates of oocytes from 20, 40, 60, 80% of sperm motility by IVF and ICSI of bovine oocytes were 47.8%~75.0%, 78.3%~90.0% and 8.7%~25.0%, 34.8%~70.0%, respectively. 3. The in vitro fertilization and cleavage rates of oocytes from 0.01, 0.02, 0.03, 0.05% of PVP concentration by microinjection of single into the bovine oocytes were 72.7%, 90.9%, 83.3%, 76.9% and 45.5%, 72.7%, 58.3%, 61.5%, respectively and these values of 0.02% addition of PVP were higher than other concentrations of PVP. 4. The in vitro fertilization and developmental rates of oocytes by IVF and ICSI methods were 63.3%~64.6%, 26.7%~29.2% and 88.2%, 47.1%, respectively. This ICSI method was improved high fertilization rates of bovined oocytes.

• Journal of Korean Society of Forest Science
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• v.98 no.5
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• pp.558-562
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• 2009

The growth, carbon and nitrogen status of container grown black pine (Pinus thunbergii) seedlings were examined at various levels of foliar fertilization (control, 0.1%, 0.2%, 0.3%). Root collar diameter, height and dry weight of black pine seedlings increased significantly with increasing levels of foliar fertilization (P<0.05). Carbon concentration in needle of black pine seedlings was significantly higher in the foliar fertilization than in the control treatments (P<0.05), while other seedling components such as stem and roots were not significantly different (P>0.05) between the foliar fertilization and the control treatments. Nitrogen concentration and content were significantly greater in the foliar fertilization than in the control treatments (P<0.05). Shoot/root ratio of black pine seedlings (needle+stem dry weight/root dry weight) was greater in the foliar fertilization (2.40-2.89) than in the control treatments (1.87). However, nitrogen use efficiency was significantly lower (P<0.05) in the foliar fertilization (28-46) than in the control (111) treatments. The results indicate that morphological characteristics and nutritional status on container grown black pine seedlings were enhanced by various levels of foliar fertilization.

• Korean Journal of Animal Reproduction
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• v.14 no.4
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• pp.245-252
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• 1990

This study was carried out to investigate the factors affecting fertilization in vitro of follicular oocytes with frozen-thawed spermatozoa in Korean Native Cattle. The bovine ovaries were obtained at a slaughter house and the follicular oocytes were recovered by aspirating the follicular fluid from the visible follicles of 3~6mm. The bovine oocytes were matured in vitro for 20~24 hours in TCM-199 containing FCS and hormones. The matured oocytes were fertilized in vitro using Percoll-separated frozen-thawed spermatozoa in BO solution. The effects of dilution and fertilization media, capacitating method, concentration of inseminated sperm and time after insemination of fertilization, were observed. The results obtained are summarized as follows : 1. The fertilization rate of frozen-thawed sperm inseminated in BO solution with caffeine and heparin together(56.4%) was higher than that of sperm inseminated in BO solution with either caffeine(10.5%) or heparin(8.9%) and without both caffeine and heparin(0%)(P<0.05). 2. The fertilization rate(56.3%) of frozen-thawed sperm inseminated in BO solution with both caffeine and heparin without preincubation was higher than that of sperm preincubated(2.9%)(P<0.05). 3. The fertilization with high concentration of frozen-thawed sperm(1.4~1.8$\times$107cells/ml) in BO solution containing caffeine and heparin resulted in higher fertilization rate, 76.7%, than the low concentration of sperm(0.8~1.0$\times$107cells/ml), 32.7%(P<0.01). 4. When the oocytes were inseminated with frozen-thawed sperm in BO solution containing caffeine and heparin without preincubation, fertilization rate increased by time and the rates were 5.9, 46.0 and 59.4% at 8, 16 and 24 hours, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.2
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• pp.93-98
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• 1993

To determine the effects of potassium($K_2O$) fertilization level(0, 100, 200 and 300 kg/ha/year) on the growth, dry matter(DM) yield, nutritive value and nitrate nitrogen($NO_3$-N) concentration of grasses grown under shading condition, this experiment was carried out in Grassland Div., LES, 1989. The plants were sampled on different growing seasons (spring, summer and autumn) and growth stages (grazing and soiling), respectively. Shade was controlled artificially ca. 45-50%, and the level of nitrogen fertilization was 200 kg/ha/year in all treatments. Higher DM was produced in spring 1, 453 kg at grazing and 2, 364 kg/ha at soiling stage, and DM production was increased with increasing level of $K_2O$. In this experiment, the optimum amount of $K_2O$ fertilizer was 200 kg/ha. The neutral detergent fiber, acid detergent fiber, DM digestibility, and relative feed value of grasses were not affected by $K_2O$ fertilization, regardless of growing season and growth stage. The $NO_3$-N concentration of grasses grown in spring was very low (ca. 1.0%), regardless of growth stage and $K_2O$ level. However, $NO_3$-N concentration was very high in summer and autumn season, also $NO_3$-N was decreased significantly with increasing level of $K_2O$ fertilization (P<0.05). Application of $K_2O$ fertilizer, therefore, is thoughs to be desirable for reducing $NO_3$-N concentration of grasses, especially in summer and autumn season. So annual split fertilization of $K_2O$ could be recommended on woodland pasture.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2000.10a
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• pp.623-628
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• 2000

This study were conducted to evaluate the mass balance of phosphorus and nitrogen with cropping in experimental pot where the mass of input concentration, and fertilization were variable. Four treatments include CSWNF, TWCF, SWNF. And these cases were compared to the control case of tap water irrigation with conventional fertilization (CONTROL). Fertilization were following conventional fertilization , N : P : K = 11kg : 7kg : 8kg. Input water loading was CSWNF (N:48.7g ,P:3.6g), TWCF(7.8g, 0.6g), SWHF(38.8g ,2.9g), TWNF(38.8g, 2.9g ) and CONTROL(0g ,0g) The result is nitrogen decrease rate; TWCF(19.2%), SWHF(14.9%), CSWNF(9.2%) and SWCF(5.6%). phosphorous decrease rate ; TWCF (10%), SWHF(3.7%), SWCF(0.9%) and CSWNF(0.3%).

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.1
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• pp.40-46
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• 1988

This study was carried out to determine the effects of nitrogen (N) fertilization levels (0, 100 and 200kg/ha) and formaldehyde (0.4,0.8 and 1.2%: w/w CM) on the chemical composition, dry matter (DM) yield and in-vitro dry matter digestibility (IVDMD) of forage rape (Brassica napus Subsp. oleifera) silage. The results obtained are summarized as follows : 1. Forage rape was a catch crop which was highly digestible and low concentration in NDF, ADF, cellulose and lignin. 2. The concentration of the water soluble carbohydrate (WSC) and buffering capacity (PK) was 17.9% and 6.77g/100g, respectively, provided the WSC to PK ratio was 2.65. 3. DM content of silage decreased as the rate of N fertilization increased, but concentration of ammonia-N decreased. IVDMD was not affected by the rate of N fertilization. 4. By the addition of formaldehyde, the pH and IVDMD were increased, but the DM, lactic acid, total organic acid and ammonia-N content of rape silage were decreased, that was effective as a silage additive for forage rape.

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.180-185
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• 2007

The effects of selenium (Se) and germanium (Ge) fertilization on the growth and quality of Chinese cabbages cultivated in spring and autumn and peppers cultivated in spring were investigated. $Se\;(Na_2SeO_4)\;and\;Ge\;(GeO_2)$ were supplied 5, 10, or 20 times in an aqueous solution of 0, 2, 4, or $8mg{\cdot}L^{-1}$ during the cultivation of Chinese cabbages and peppers. The fresh weight of Chinese cabbages increased by Ge fertilization with high concentration. But it was not affected by Se fertilization. The content of vitamin C increased by 10 times application with $4mg{\cdot}L^{-1}$ of Se or Ge. The concentration of Se in Chinese cabbage increased according to increasing concentration of Se fertilization. Se concentration was higher in the outer leaves than in the inner leaves. Se concentration in the mesophyll was higher than that in the midrib. Ge fertilization increased the uptake and concentration of Ge in autumn-cultivated Chinese cabbages. Se and Ge fertilization did not affect the fresh weight of peppers. The content of vitamin C in pepper increased by 20 times application of $2mg{\cdot}L^{-1}$ of Se. Vitamin C content in red peppers was twice as much as in green peppers.