• 제목/요약/키워드: fermenters

검색결과 26건 처리시간 0.018초

The Production of Anti-cancer Substances by in vitro Grown Cultures of Panax ginseng C.A. Meyer

  • Yang, Deok-Chun;Park, Kyung-Hwa;Kim, Yong-Hae;Yoon, Eui-Soo;Kang, Tae-Jin;Park, Kwang-Tae
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 1999년도 The 6th International Symposium on the Development of Anti-Cancer Resource from Plants
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    • pp.46-57
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    • 1999
  • Ginseng(Panax ginseng C.A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng callus and hairy roots grow vigorously and may Produce the same or more biologically active compounds for human health than natural ginseng roots. Therefore, ginseng callus and hairy roots can be used for commercial purposes. Polyacetylene, one of anti-cancer compounds in ginseng, was not detected in the callus cultured on the medium containing 2, 4-B, but cells derived from the callus growth was excellent, The ginseng calli cultured on the medium containing 2mg11 CPA and 0.05mg/1 BA was grown vigorously and produced panaxydol, one of ginseng polyacetylene. The biosynthesis of polyacetylene in callus was not affected by addition of NAA and sucrose in media. The SH medium was better than the MS medium for ginseng callus growth and biosynthesis of panaxydol. Another ginseng anti-cancer compounds, ginsenoside-Rg$_3$, Rh$_1$and Rh$_2$ were detected in ginseng hairy roots by heat treatment. Those of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes Rl000 $A_4$T in dark condition after one month of culture. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark(22$^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5 ι Erlenmeyer flasks, 1ι roller drums, 10ι jar-fermenters, and especially in 20ι air-lift .culture vessels. All heat treatments had remarkably different ginsenoside contents. Eleven ginsenosides were determined in heat treatment, eight in freeze dried hairy roots. Contents of ginsenoside-Rbl , Rb2, Rc, Rd. Re, Rf, and Rg$_1$tested in all heat treatments were less than those of freeze dried hairy roots. Contents of glnsenoside-Rg$_2$ in heat treatment for 1 hour at 105$^{\circ}C$ was 4.92mg/g dry wt, 3.9 times higher than 1.27 mg/g dry wt of freeze dried hairy roots. The optimum condition of heat treatment for the production of ginsenoside-Rg$_3$and Rhl was 2 hours at 105$^{\circ}C$, and ginsenoside content was 2.58mg/g dry wt and 3.62mg/g dry wt, respectively. The production of ginsenoside-Rh2 was the highest in heat treatment for 2 hours at 105$^{\circ}C$ among treatments examined, and ginsenoside-Rh$_2$content was 1.08mg/g dry wt.

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폐지를 이용한 혐기성 산 발효 및 메탄발효 특성 (Anaerobic Acid and Methane Fermentation using Paper Wastes)

  • 조건형;김중곤;김성준;김시욱
    • KSBB Journal
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    • 제18권4호
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    • pp.318-323
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    • 2003
  • 본 연구는 셀룰로오스 성분의 분해가 용이하지 않은 폐지 를 이용하여 유기산 및 메탄으로 전환시킬 수 있는지의 여부 에 대하여 연구하였다. 유기산 생성 회분식 실험에서 초기 건조중량 15 g의 폐신문지와 종이박스를 음식페기불 산 발 효액과 1 1로 혼합하여 주입하였을 때 생성된 유기산의 총량은 신문지와 종이박스가 각각 2461과 4978 mg/L로 나 타났다. 메 탄발효 회분식 반응에서 초기 건조중량 15 g의 신 문지와 종이박스 폐지를 주입하였을 때 24일 후 tCOD 제거 율은 각각 60.9와 62.4%를 나타내었고 생산된 바이오 가스양 은 각각 6.95와 6.43 L이었다. 총 고형불 (TS)의 변화는 신문 지와 종이박스가 각각 34.8 과 33.4% 정도 감소함을 알 수 있었고, 휘발성 고형물 (VS)의 변화는 신문지와 종이박스가 각각 40.0 과 39.2% 정도 감소함올 알 수 있다 pH는 20일 이후부터 7.5로 일정하게 유지되어 메탄발효가 적절히 진행 되는 것으로 확인되었다. 반 연속식 실혐의 경우 산 발효조 에서 2일, 메탄 발효조에서 12일간 체류하변서 신문지와 종 이박스의 tCOD 제거 효율은 각각 64.7과 65.0%를 나타냈다. 각각의 일일 바이오 가스 생산량은 g당 0.31과 0.30 L로 나 타났으며 바이오 가스 중 메탄함량은 57.3과 56.2%로 나타났 다. 공정의 안정화가 이루어졌다고 판단되는 25일 이후의 pH 는 혐기성 산 발효조와 메탄 발효조에서 각각 5.0과 7.5로 일 정하게 나타났다

가축 사료에서 유당비분해 그람음성균의 분리 및 분리균의 항생제 감수성 (Isolation of lactose non-fermenting Gram negative bacilli from animal feedstuffs and antibiotics susceptibility of isolates)

  • 허부홍;서석열;이병종;엄성심;송희종;채효석;김진환
    • 한국동물위생학회지
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    • 제20권2호
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    • pp.143-150
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    • 1997
  • Total viable cells and lactose non-fermenting cells were counted from animal feedstuffs (n=65). And isolation of Gram negative lactose nonfermenting enterobacteria and antibiotics susceptibility of isolates were performed. 1. The ranges of total viable cells / lactose non-fermenters in animal feedstuffs from Korean cattle were counted as 9$\times$$10^4$-1$\times$$10^7$ / 1$\times$$10^2$-6$\times$$10^3$, milking cow as 1$\times$$10^4$-2$\times$$10^8$ / 2$\times$$10^2$-8$\times$$10^3$, pig as 1$\times$$10^4$-1$\times$$10^6$ / 2$\times$$10^2$-6$\times$$10^3$, and chicken as 7$\times$$10^4$-1$\times$$10^9$ / 4$\times$$10^2$-1$\times$$10^5$ cfu/g, respectively. 2. Among the 214 isolates from feedstufs, 87 from Chinan(n=23), 66 from Changsu (n=23) and 61 from Mooju(n=19) were isolated. Of these isolates, 60 from pigs (n: 19), 51 from milking cows(n=15), 45 from chikens(n=11) and 58 from Korean cattle(20) were isolated. 3. Among the 6 genuses of Gram negative lactose nonfermenting enterobacili, Salmonella sp, Y pseudotuberculosis, Ent agglomerans and Sal choleraesuis were frequently encountered. 4. A majority of isolates were sensitive to 19 antibiotics, singly or in combination. These isolates were completely susceptible to Cp, Gm, Imp and Pi, 93% to Ak and To, 73% to Cax and Ts, 66% to Cft and Tim, 46-53% to Caz, Cf and Cz, 33-40% to Am, Azt, Cfz and Ti, and 6% to Cfx, in order, but not susceptible to Crm. 5. Among the antibiotic resistant strains, a total of 23 resistant patterns was noted, and of these Crm 40(18.7%), Am Cf Cfx Cfz Crm Ti 27(12.6%), each of Azt Ctx Crm and Azt Cax Caz Cft Cfx Crm 22(10.3% ) were frequently encountered.

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Bacillus amyloliquefaciens ISP-5 균주의 배지 최적화 및 상추를 이용한 식물 생장 촉진 평가 (Medium optimization for growth of Bacillus amyloliquefaciens ISP-5 strain and evaluation of plant growth promotion using lettuce)

  • 최강현;서선일;박해성;임지환;김평일
    • Journal of Plant Biotechnology
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    • 제49권4호
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    • pp.356-361
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    • 2022
  • 본 연구에서는 Bacillus amyloliquefaciens ISP-5 균주의 최적배지조성을 확인하기 위해 5종의 탄소원(glucose, fructose, sucrose, maltose, corn starch)과 5종의 질소원(yeast extract, peptone, tryptone, soytone, soy bean flour)을 이용하여 각각의 생육을 측정하였다. 그 결과 glucose와 soy bean flour를 각각 최적 탄소원과 질소원으로 확립하였으며 최적 배지를 이용하였을 때 상업용 배지(TSB)에 비해 약 2500배의 생육을 확인할 수 있었다. B. amyloliquefaciens ISP-5 배양액을 상추에 엽면 살포하였을 때 상추의 엽폭과 엽장은 대조군에 비해 8.6%, 12.9%의 증가하였으며, 생체중과 건물중은 대조군에 비해 각각 24.2%, 23.9% 증가하였다. 이러한 결과들을 종합해보았을 때 B. amyloloiquefaciens ISP-5 균주를 이용한 미생물 제제는 상추용 식물생장촉진제로써 활용할 수 있을 것이라 기대된다.

"고사십이집(攷事十二集)"의 조리가공에 관한 분석적 연구(2) (A Study on the Cooking in 'The Kosa-sibi Jip' (2))

  • 김성미
    • 동아시아식생활학회지
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    • 제4권3호
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    • pp.1-19
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    • 1994
  • In this paper, twenty-five kinds of food presented in Sooljip(戌集) 5 and 6 of Food collections of 'Kosa-sibi Jip(攷事十二集)' have been classified into four : Staple food, subsidiary food, Tuck(rice cake) and Han-gwa(Korean confectionery), and Tang-jng and tea. Cooking processes have been examined and scientifically analyzed in terms of cooking, Fourteen kinds of Jook (thick gruel with cereal) as well as Urak-Jook were presented among the methods of making Jook, one of staple foods. Milk and ground rice were boiled together into Urak-Jook, which was nutritious because of carbohydrate, added to milk. Hong-sa Myun was mode of ground shrimps, ground bean, ground rice and flour which were kneaded together. It was a nutritiously balanced food. Nineteen kinds of Kimchi presented in this book were classified by the recipes. The five of Jook-soon Ja, U-so Ja, Tam-bok Ja and Jo-gang were made by adding red malt and cereals(boiled rice or candies). Jo-gang, Jo-ga and Jo-gwa-chae were made by adding salt and rice wine. With salt and fermenters added, eight were made. Chim-jup-jeo-ga was made by adding Jang(soy-bean sauce) and the inner chaff of wheat instead of salt. The four of Ka-za-san, Hwang-gwa-san, Tong-gwa-san and Jo-gang were made by adding salt and vinegar. Jo-gang was made by adding salt, rice wine, residue of rice wine and candies. The four of Kae-mal-ga, Ku-cho-chim-chae, Un-gu-hwa and Suk-hwa-chim-chim-chae were made by adding salt and spices. San-got-Kimchi was made without salt. San-got-Kimchi and Suk-hwa-chim-chae were made originally in Korea. Suk-hwa-chim-chae, in particular, was first classified as a kind of Kimchi in this book and oysters were added, which is notable. Pork could be preserved longer when smoked oven the weak fire of thatch ten days and nights. Dog meat was sauced and placed on the bones in a pot. A porcelain was put on the top of the pot. Flour paste sealed the gap between the porcelain and the pot. Some water was poured into the porcelain, and the meat was steamed, with two or three thatched sacks burned, which was a distilled dry steaming. This process has been in use up to now. Various cooking methods of chicken were presented from in Umsik-dimi-bang to in Chosun Musang Sinsik Yori Jebup. These methods were ever present regardless of ages. Such measuring units as Guin(斤) and Nyang(兩) were most frequently used in cooking processes of this book, except in case of Jang(soy bean sauce), vinegar and liquor. Twenty eight kinds of kitchenware and cookers were used, of which porcelains wee most used and pans and sieves followed. The scientific eight cooking methods were as follows. First, salt was refined through saturated solution. Next, it was recommended Hong-sa Myun containing shrimps should not be taken along with pork, which is thought to be a proper diet in terms of cholesterol contained by shrimps and pork. Third, meat was coated with thin gruel and quickly roasted and cleared of the dried gruel membrane, which prevented nutrients from exuding and helped to make the meat well-done. Fourth, The fruit of paper mulberry trees has the protease which can soften meat. Therefore when meat was boiled with th fruit of paper mulberry trees, it can be softened easily. Fifth, pork was smoked over the weak fire of thatch. Sixth, in cooking dog meat, distilled dry steaming raised the boiling point and made it possible to preserve meat longer. Seventh, in boiling the sole of a bear, lime was added, which made meat tender by making the pH lower or higher than that of raw meat. Finally, in boiling down rice gluten, a porcelain in the pot prevented boiling over the brim, which is applied to pots in which to boil medical herbs.

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Aspergilus Oyzae 혹은 Saccharomyces Cerevisiae를 첨가하여 제조한 맥주박 위주 발효사료가 반추위 미생물에 미치는 영향 (Effects of Brewery Meal-Based Fermented Feedstuff Supplemented with Aspergillus Oryzae or Saccharomyces Cerevisiae on Ruminal Microorganism)

  • 박병기;라창식;김병완;오진석;신종서
    • 한국초지조사료학회지
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    • 제25권4호
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    • pp.307-318
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    • 2005
  • 본 연구는 Aspergillus oryzae(AO) 혹은 Saccharomyces cerevisiae(SC)를 첨가하여 제조한 맥주박 위주 발효사료의 급여가 한우의 반추위내 미생물에 미치는 영향을 조사하기 위해 실시하였다. 공시동물은 반추위 cannula가 장착된 한우 암소 2두를 이용하였다. 시험은 시판 배합사료 $715\%$ 및 corn silage $28.5\%$ 급여구(대조구), 시판 배합사료 $45.0\%$, AO 첨가 발효사료 $26.5\%$ 및 com silage $28.5\%$ 급여구(TAO)와 시판 배합사료 $45.0\%$, SC 첨가 발효사료 $26.5\%$ 및 corn silage $28.5\%$ 급여구(TSC)의 3처리구로 구분하여 수행하였다. Total viable bacteria(p<0.05), 혐기성 곰팡이 및 protozoa(p<0.05)의 수는 대조구에 비해 TAO 및 TSC에서 높았다. 단백질 분해 박테리아(p<0.05), 섬유소 분해 박테리아 및 xylan 이용 박테리아 수는 대조구에 비해 TAO 및 TSC구에서 높은 경향이었다 Protozoa의 건물회수율은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 총 미생물 및 protozoa의 조단백질 함량은 대조구 및 TAO구에 비해 TSC구에서 높았다(p<0.05). 박테리아의 조단백질 함량은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 총 미생물의 조지방 함량은 대조구 및 TSC구에 비해 TAO구에서 높았으며 (p<0.05), protozoa 및 박테리아의 조지방 함량은 대조구 및 TAO구에 비해 TSC구에서 높았다(p<0.05). 총 미생물의 필수아미노산 비율은 TAO 및 TSC구에 비해 대조구에서 높았다. 박테리아의 methionin 및 alanine 비율은 대조구에 비해 TAO 및 TSC구에서 높았다(p<0.05). 이상의 결과에서 AO 혹은 SC를 첨가하여 제조한 발효사료의 급여는 반추위내 미생물 수 및 균체 조성 변화에 영향을 미치는 것으로 판단된다.