• Title/Summary/Keyword: feces

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Detection of Lawsonia intracellularis in swine feces by polymerase chain reaction (돼지분변에서 PCR에 의한 Lawsonia intracellularis 검색)

  • 장성준;김정화;김영태;김기향;김중규;김영욱;최일영
    • Korean Journal of Veterinary Service
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    • v.24 no.1
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    • pp.43-50
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    • 2001
  • Swine proliferative enteritis(SPE) caused by inかsoma intracellularis is a common enteric disaese of grower and finisher pig. Swine affected with SPE show variable clinical signs including diarrhea, weight loss, aberrant growth and death. The characteristic lesion of ileitis at necropsy is marked thickening of the last section of the small intestine. The inner lining of the thickened intestine proliferates almost like a cancer and curved rod bacteria(L intracellularis) are always seen inside the intestinal wall. Infected swine shed the organism in the feces. Isolation and growth of pure L intracellularis in vitro requires a suitable cell culture. This procedure is difficult and not a practical means of diagnosis, thus the polymerase chain reaction(PCR) test of feces can be used to determine whether a pig is shedding the infective organism. A sensitive assay based on amplification of a 319bp DffA fragment of the L intracellularis of Swine proliferative enteritis was attempted for the detection of the organism in the 62 feces of swine. L intracellularis was identified on three herds and detected in 6 fecal samples, representing a infection rate of 9.7%. The PCR was very sensitive and specific on the individual level. The PCR technique could be very useful for the diagnosis of this disease.

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The Efficacy of Albendazole, Fenbendazole and Metronidazole for Treatment of Canine Giardia (개의 Giardia 감염에 따른 Albendazole, Fenbendazole, Metronidazole의 치료 효과)

  • Kim Nam-soo;Chon Seung-ki
    • Journal of Veterinary Clinics
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    • v.22 no.3
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    • pp.239-243
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    • 2005
  • Current treatments for canine Giardia infections include albendazole, fenbendazole and metronidazole in the most cases. To compare efficacy of these antimicrobial drugs for treating naturally infected giardiasis in dogs, we used the zinc sulfate concentration technique(ZSCT) to figure out naturally infected with Giardia. In this study, feces were collected from 150 asymptomatic dogs at a kennel, 28 asymptomatic Giardia infected dogs were used by the allocated four group to receive albendazole(group A, 25 mg/kg, PO, bid, 7 days), fenbendazole(group F, 50 mg/kg, PO, sid, 7 days), metronidazole (group M, 50 mg/kg, PO, sid, 7 days), nontreated control(group NC) respectively. Fecal samples from each dog were examined for Giardia cysts within 3 days of the last treatment. Giardia cysts were not detected in the feces of any dog in group F. Cysts were detected in the feces 1 of 7 dogs in group A or in the feces 2 of 7 dogs in group M. In the group NC, cysts were detected in the feces of 7 of 7 dogs. Finally, there was significant difference for efficacy between antimicrobial drugs against Giardia in dogs. Fenbendazo1e is the most efficacy to have giardiacidal activity.

Etiology and clinical aspects of diarrhea of Korean native calves during the suckling period (한우 송아지의 포유기간 중의 설사발생에 관한 연구)

  • Kim, Doo;Lyoo, Young-soo;Lyoo, Han-sang;Yoon, Chung-keun
    • Korean Journal of Veterinary Research
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    • v.30 no.2
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    • pp.255-260
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    • 1990
  • This study was conducted to examine the enteropathogens and clinical aspects of diarrhea of the 211 Korean native calves during the suckling period. The experimental results were summerized as follows: 1. The 206 Korean native calves (97.6%) were affected with diarrhea during the suckling period. 2. Of 156 diarrheal feces tested, Rotavirus were detected from 108(69.2%) feces of $K^+99$ E coli were detected from 23(14.7%) feces and Eimeria spp were detected from 33(21.2%) feces. But Salmonella spp and Cryptosporidium spp were not detected. 3. Rotavirus were detected from 2 to 84 days of life, $K^+99$ E coli were detected from 5 to 54 days and Eimeria spp were detected from 33 to 84 days of life. 4. Physical appearances of the diarrheal feces were not affected specifically according to the enteropathogens, but were affected by the severity of diarrhea and the diets.

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Comparison of Volatile Organic Compound and Volatile Fatty Acid Concentration in Feces and Urine of Finishing Pigs (초지환원용 비육돈의 분과 뇨에 있어서 휘발성유기물과 휘발성지방산 농도 비교)

  • Cho, Sung Back;Hwang, Ok Hwa;Yang, Seung Hak;Kwag, Jeong Hoon;Choi, Dong Yun;Yang, Sung Bong;Kim, Doo Hwan;Park, Sung Kwon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.2
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    • pp.120-124
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    • 2014
  • This study was performed to compare the level of odorous compounds in feces and urine of finishing pigs. Feces and urine from 16 finishing pigs were separately collected for 28-d. Concentrations of volatile organic compound (VOC; phenols and indoles) and volatile fatty acid (VFA; SCFA and BCFA) were measured in feces and urine. Amount of phenols and p-cresol was higher (P<0.05) in urine than in feces. Urinal levels of phenols and p-cresol were 257.8 ppm and 250.9 ppm, and those of fecal phenols and p-cresol were 0.50 ppm and 0.05 ppm, respectively. There was no difference in concentration of indoles from feces (1.0 ppm) and urine (1.8 ppm). Short chain fatty acid (SCFA) level in urine was higher (P<0.05) than in feces showing 4,547 ppm and 863 ppm, respectively. Proportion of acetic acid to total SCFA was higher in urine (94%) than in feces (66%). However, level of branched fatty acid (BCFA) was greater in feces (118 ppm) compare to that of urine (87 ppm). Odorous compounds analyzed in the current study, phenols and SCFA, were contained more in urine than in feces. Greater amount of VFA is typically found in feces than in urine since it is generated in the large intestine. However, urine contained more VFA than feces in the current study. Therefore, it will be necessary to exploit odor reducing techniques especially for pig urine as grassland fertilizer.

Determination of Thiamine in Urine and Feces of the Rats by the Fluorometric Method (형광법(螢光法)에 의(依)한 Rat 분뇨중(糞尿中)의 Vitamin $B_1$의 정량(定量))

  • Lee, Hyun-Ki;Rhee, Sook-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.3 no.1
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    • pp.1-5
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    • 1974
  • Two 3-month old Wistar Strain male rats (weights was 270g and 340g respectively) were used as samples in order to determine the daily excretion of thiamine contained in urine and feces by the thiochrome reaction. The results were as follows. 1. Daily excretion of urine was 5,2 cc per 100 grams of body weight and that of thiamine was $18.65\;{\gamma}$ per 100 grams of plasma. 2. Daily excretion of feces was 2.4 grams per 100 grams of body weight and that of thiamine was $7.24\;{\gamma}$ per 100 grams of plasma. 3. Daily excretion of thiamine contained in urine is about twice the amount of thiamine excretion contained in feces. Thus, it can be concluded that more of the excretion of thiamine was mainly excreted through urine.

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The Reproductive Patterns of Endangered Captive Orangutans by Analysing the Sex Hormones in Feces (분변내 성호르몬의 분석을 통한 멸종위기 사육 오랑우탄에서의 번식형태)

  • Jung, So-Young;Kim, Bo-Sook;Yoon, Yong-Dal
    • Journal of Veterinary Clinics
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    • v.30 no.1
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    • pp.22-26
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    • 2013
  • To monitor the reproductive patterns of endangered orangutan, Time-resolved fluorescence immuno assay (TR-FIA) were used to analyze metabolites of sex hormones in feces. Orangutan had long-term interbirth intervals (amenorrhea) during lactation period in which the secretion of sex hormones was repressed. The concentration of progesterone in the serum of pregnant orangutan was 30fold higher than that in non-pregnant orangutan. However, the concentration of hCG during pregnant period was different from the result of other primates. The present study suggested that age is not the important factor in determining the reproduction capability, because it is rather greatly influenced by the combination of various factors. Tracing metabolites of sex hormones in orangutan feces seems to be provide the effective solution for the infertility in orangutan. This study result shows the basic data in operating conservation project for endangered orangutan.

Feces Production Rate of Abalone (Haliotis discus hannai) fed Undavia pinnatifida (미역 (Undaria pinnatifida)을 섭취 한 참전복(Haliotis discus hannai)의 분 배출률)

  • Park, Jeong-Hwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.6
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    • pp.353-358
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    • 2005
  • The present study investigated the feces production rate and fecal nitrogen production ratio of abalone, Haliotis discus hannai fed seaweed, Undaria pinnatifida. The abalone, with shell lengths of 3 cm, 5 cm, and 7 cm, were tested at temperatures of $12^{\circ}C$, $16^{\circ}C$, and $20^{\circ}C$ in a semi-recirculating aquaculture system. Under the given experimental conditions, the weight specific feces production rate (FPw) of the abalone increased with the decrease of shell length and the increase of temperature. The relationship of the FPw was. $FPw=-3.092+206.573/T - 1916.8/T^2+0.141L -0.037L^2(r^2=0.9412)$. In addition the abalone produced 17.8-22.5% of fecal material against ingested seaweed, Undaria pinnatifida, and $18.4-22.4\%$ of nitrogen ingested total nitrogen.

Production and Characteristics on Manure and Urine of Lactating Sow (포유 모돈 분뇨의 배설량과 이화학적 특성)

  • 김형호;전병수
    • Journal of Animal Environmental Science
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    • v.5 no.3
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    • pp.171-174
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    • 1999
  • This study was conducted to investigate the quantity and characteristics of manure and urine produced by lactating sow fed commercial diet for 24 days. 3 way cross bred 10 lactating sows with litters were alloted at farrowing pen. Crude protein and digestible energy were 14% and 3,467 kcal in diet, respectively. Average body weight of sow was 237.5kg. Feces with urine was produced 6.98kg per day by lactating sow ranging from 5.72 to 7.93kg. Manure solid was produced 2.75 kg per day ranging from 2.17 to 3.87kg and urine 4.23kg per day ranging from 3.55 to 4.06kg, respectively. Dry matter of feces and urine were 29.91 and 1.54%, respectively. Nitrogen and phosphorus contents in fresh feces were 0.86 and 0.30%, respectively. Nitrogen content in urine was 0.86, and phosphorus in urine was 0.47%. pH in manure was 6.73 and in urine was 8.38 respectively. Biochemical oxygen demand and suspended solid in urine were 5,196 and 308mg/$\ell$, respectively.

Treatment of Thermoactinomyces sp. to Application of Poultry Feces (계분을 이용하기 위한 Thermoactinomyces sp. 균처리)

  • Choi, Moo-Young;Lee, Eun
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.530-534
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    • 1990
  • A strain of actinomycetes, Thermoactinomyces sp. CH-53, was isolated from manure and composted livestock feces. Actinornycetes-feed additive was prepared with the solid wheat bran medium of Thermoactinomyces sp. CH-53 that grew vigorously on unsterilized poultry feces at $50^{\circ}C$. pH 6.5- 9.5 and moisture content of 55-65% and added at a rate of 1% (wtlwt) to the commercially assorted feed to be fed poultry. The excreted feces contained $10^7-10^8$. Thermoactinomyces sp. CH-53 cells per gram. Poultry feces malodour was got rid of during treatment. The effect on plant growth was evaluated on the basis of the amount of nitrogen as fertilizer under a loading of 0.2g N1600g soillpot. A11 samples were showed a promotion effect for plant growth. The treated poultry feces added from O.lg to 0.4g total nitrogen per 600g soil in a pat increased the growth of Brassica rapa var. perui-ridis.

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Production of Biofertilizer from the Rice Straw Mixed with Hen Feces with Thermoactinomycetes vulgaris (볏짚과 계분(鷄糞)의 혼합물로 부터 Thermoactinomycetes vulgaris에 의한 생물비료(生物肥料)의 제조(製調))

  • Choi, Moo-Young;Kang, Shin-Jyung;Lee, Jae-Sung
    • Applied Biological Chemistry
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    • v.31 no.1
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    • pp.100-105
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    • 1988
  • A biofertilizer, having been deordorized and showing promotive effect on plant growth, was manufactured from the rice straw and hen feces by use of Thermoactinomycetes vulgaris. This strain grew vigorously on rice straw mixed with unsterilized hen feces at $50^{\circ}C,\;pH\;8.0{\sim}8.5$ and moisture content of 60% and got rid hen feces of malodour during treatment. The growth of plant(Brassica raga var. previdis) was experimented on humic volcanic ash soil, using pot in thermostatically controlled greenhouse. The biofertilizer was applied as N-fertilizer and air-dried lien feces or ammonium sulfate were used for comparison with the biofertilizer. The effect on. plant growth was evaluated on the basis of the amount of nitrogen as fertilizer, under a loading of 0.1g N/pot, all samples showed a promotion effect of plant growth. But ammonium sulfate and air-dried hen feces inhibited plant growth at the nitrogen content over 0.2 and 0.4g N/pot, respectively, whereas the biofertilizer showed a good promotion effect on plant growth without growth inhibition even at nitrogen content of 0.8g N/pot.

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