• 제목/요약/키워드: feces

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돼지분변에서 PCR에 의한 Lawsonia intracellularis 검색 (Detection of Lawsonia intracellularis in swine feces by polymerase chain reaction)

  • 장성준;김정화;김영태;김기향;김중규;김영욱;최일영
    • 한국동물위생학회지
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    • 제24권1호
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    • pp.43-50
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    • 2001
  • Swine proliferative enteritis(SPE) caused by inかsoma intracellularis is a common enteric disaese of grower and finisher pig. Swine affected with SPE show variable clinical signs including diarrhea, weight loss, aberrant growth and death. The characteristic lesion of ileitis at necropsy is marked thickening of the last section of the small intestine. The inner lining of the thickened intestine proliferates almost like a cancer and curved rod bacteria(L intracellularis) are always seen inside the intestinal wall. Infected swine shed the organism in the feces. Isolation and growth of pure L intracellularis in vitro requires a suitable cell culture. This procedure is difficult and not a practical means of diagnosis, thus the polymerase chain reaction(PCR) test of feces can be used to determine whether a pig is shedding the infective organism. A sensitive assay based on amplification of a 319bp DffA fragment of the L intracellularis of Swine proliferative enteritis was attempted for the detection of the organism in the 62 feces of swine. L intracellularis was identified on three herds and detected in 6 fecal samples, representing a infection rate of 9.7%. The PCR was very sensitive and specific on the individual level. The PCR technique could be very useful for the diagnosis of this disease.

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개의 Giardia 감염에 따른 Albendazole, Fenbendazole, Metronidazole의 치료 효과 (The Efficacy of Albendazole, Fenbendazole and Metronidazole for Treatment of Canine Giardia)

  • 김남수;전승기
    • 한국임상수의학회지
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    • 제22권3호
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    • pp.239-243
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    • 2005
  • Current treatments for canine Giardia infections include albendazole, fenbendazole and metronidazole in the most cases. To compare efficacy of these antimicrobial drugs for treating naturally infected giardiasis in dogs, we used the zinc sulfate concentration technique(ZSCT) to figure out naturally infected with Giardia. In this study, feces were collected from 150 asymptomatic dogs at a kennel, 28 asymptomatic Giardia infected dogs were used by the allocated four group to receive albendazole(group A, 25 mg/kg, PO, bid, 7 days), fenbendazole(group F, 50 mg/kg, PO, sid, 7 days), metronidazole (group M, 50 mg/kg, PO, sid, 7 days), nontreated control(group NC) respectively. Fecal samples from each dog were examined for Giardia cysts within 3 days of the last treatment. Giardia cysts were not detected in the feces of any dog in group F. Cysts were detected in the feces 1 of 7 dogs in group A or in the feces 2 of 7 dogs in group M. In the group NC, cysts were detected in the feces of 7 of 7 dogs. Finally, there was significant difference for efficacy between antimicrobial drugs against Giardia in dogs. Fenbendazo1e is the most efficacy to have giardiacidal activity.

한우 송아지의 포유기간 중의 설사발생에 관한 연구 (Etiology and clinical aspects of diarrhea of Korean native calves during the suckling period)

  • 김두;유영수;유한상;윤충근
    • 대한수의학회지
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    • 제30권2호
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    • pp.255-260
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    • 1990
  • This study was conducted to examine the enteropathogens and clinical aspects of diarrhea of the 211 Korean native calves during the suckling period. The experimental results were summerized as follows: 1. The 206 Korean native calves (97.6%) were affected with diarrhea during the suckling period. 2. Of 156 diarrheal feces tested, Rotavirus were detected from 108(69.2%) feces of $K^+99$ E coli were detected from 23(14.7%) feces and Eimeria spp were detected from 33(21.2%) feces. But Salmonella spp and Cryptosporidium spp were not detected. 3. Rotavirus were detected from 2 to 84 days of life, $K^+99$ E coli were detected from 5 to 54 days and Eimeria spp were detected from 33 to 84 days of life. 4. Physical appearances of the diarrheal feces were not affected specifically according to the enteropathogens, but were affected by the severity of diarrhea and the diets.

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초지환원용 비육돈의 분과 뇨에 있어서 휘발성유기물과 휘발성지방산 농도 비교 (Comparison of Volatile Organic Compound and Volatile Fatty Acid Concentration in Feces and Urine of Finishing Pigs)

  • 조성백;황옥화;양승학;곽정훈;최동윤;양성봉;김두환;박성권
    • 한국초지조사료학회지
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    • 제34권2호
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    • pp.120-124
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    • 2014
  • 초지 또는 농경지에 살포되는 퇴비와 액비에 의해 발생될 수 있는 악취민원에 대처하기 위하여, 돼지의 분과 뇨에 존재하는 악취물질 농도를 비교 분석하였다. 1. 비육돈의 분과 뇨에 함유된 휘발성유기물의 농도 비교 페놀, p-크레졸 및 페놀류 농도는 분 보다 뇨에서 높았으며 (p<0.05), 분 보다 뇨에 축적된 비율이 페놀은 138배, p-크레졸은 545배 높았다. 인돌 농도는 분 보다 뇨에서 높았으며, 스카톨은 뇨 보다 분에서 높았다 (p<0.05). 휘발성유기물은 대부분 뇨로 배설되었으며, 이 중 농도 및 악취강도가 가장 높은 물질인 p-크레졸이 뇨에 많이 함유되어 있다. 돼지 분뇨를 고액분리 하였을 때 퇴비로 이용되는 고체보다 액비로 이용되는 액상물질에서 악취가 훨씬 강할 것으로 예측된다. 2. 비육돈의 분과 뇨에 함유된 휘발성지방산의 농도 비교 아세트산과 단쇄지방산 농도는 분 보다 뇨에서 높았으며 (p<0.05), 부티르산과 프로피온산은 차이가 없었다 (p>0.05). 이성체지방산 중 I-부티르산의 농도는 뇨 보다 분에서 높았지만 (p<0.05), I-발레르산과 이성체지방산은 차이가 없었다 (p>0.05). 휘발성지방산 중에서 아세트산과 프로피온산은 분에서 각각 65와 20%, 뇨에서는 각각 93과 4%로 휘발성지방산의 대부분을 차지하였지만 아세트산과 프로피온산을 포함하는 단쇄지방산은 악취강도가 낮다. 반면에 악취강도가 상대적으로 높은 이성체지방산은 분과 뇨 간에 차이가 없기 때문에 휘발성지방산이 악취강도에 미치는 영향은 크지 않을 것으로 판단된다. 이상의 연구결과를 종합하면, 돼지 분뇨의 악취물질 중 악취강도가 높은 p-크레졸은 뇨에 많이 함유되어 있기 때문에 퇴비와 액비 생산과정 중 액상처리 시 악취강도가 매우 높을 것으로 추정된다. 다만, 악취강도는 휘발성지방산의 농도 변화에 따라 차이가 있을 것으로 생각된다.

형광법(螢光法)에 의(依)한 Rat 분뇨중(糞尿中)의 Vitamin $B_1$의 정량(定量) (Determination of Thiamine in Urine and Feces of the Rats by the Fluorometric Method)

  • 이현기;이숙희
    • 한국식품영양과학회지
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    • 제3권1호
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    • pp.1-5
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    • 1974
  • Two 3-month old Wistar Strain male rats (weights was 270g and 340g respectively) were used as samples in order to determine the daily excretion of thiamine contained in urine and feces by the thiochrome reaction. The results were as follows. 1. Daily excretion of urine was 5,2 cc per 100 grams of body weight and that of thiamine was $18.65\;{\gamma}$ per 100 grams of plasma. 2. Daily excretion of feces was 2.4 grams per 100 grams of body weight and that of thiamine was $7.24\;{\gamma}$ per 100 grams of plasma. 3. Daily excretion of thiamine contained in urine is about twice the amount of thiamine excretion contained in feces. Thus, it can be concluded that more of the excretion of thiamine was mainly excreted through urine.

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분변내 성호르몬의 분석을 통한 멸종위기 사육 오랑우탄에서의 번식형태 (The Reproductive Patterns of Endangered Captive Orangutans by Analysing the Sex Hormones in Feces)

  • 정소영;김보숙;윤용달
    • 한국임상수의학회지
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    • 제30권1호
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    • pp.22-26
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    • 2013
  • To monitor the reproductive patterns of endangered orangutan, Time-resolved fluorescence immuno assay (TR-FIA) were used to analyze metabolites of sex hormones in feces. Orangutan had long-term interbirth intervals (amenorrhea) during lactation period in which the secretion of sex hormones was repressed. The concentration of progesterone in the serum of pregnant orangutan was 30fold higher than that in non-pregnant orangutan. However, the concentration of hCG during pregnant period was different from the result of other primates. The present study suggested that age is not the important factor in determining the reproduction capability, because it is rather greatly influenced by the combination of various factors. Tracing metabolites of sex hormones in orangutan feces seems to be provide the effective solution for the infertility in orangutan. This study result shows the basic data in operating conservation project for endangered orangutan.

미역 (Undaria pinnatifida)을 섭취 한 참전복(Haliotis discus hannai)의 분 배출률 (Feces Production Rate of Abalone (Haliotis discus hannai) fed Undavia pinnatifida)

  • 박정환
    • 한국수산과학회지
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    • 제38권6호
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    • pp.353-358
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    • 2005
  • The present study investigated the feces production rate and fecal nitrogen production ratio of abalone, Haliotis discus hannai fed seaweed, Undaria pinnatifida. The abalone, with shell lengths of 3 cm, 5 cm, and 7 cm, were tested at temperatures of $12^{\circ}C$, $16^{\circ}C$, and $20^{\circ}C$ in a semi-recirculating aquaculture system. Under the given experimental conditions, the weight specific feces production rate (FPw) of the abalone increased with the decrease of shell length and the increase of temperature. The relationship of the FPw was. $FPw=-3.092+206.573/T - 1916.8/T^2+0.141L -0.037L^2(r^2=0.9412)$. In addition the abalone produced 17.8-22.5% of fecal material against ingested seaweed, Undaria pinnatifida, and $18.4-22.4\%$ of nitrogen ingested total nitrogen.

포유 모돈 분뇨의 배설량과 이화학적 특성 (Production and Characteristics on Manure and Urine of Lactating Sow)

  • 김형호;전병수
    • 한국축산시설환경학회지
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    • 제5권3호
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    • pp.171-174
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    • 1999
  • This study was conducted to investigate the quantity and characteristics of manure and urine produced by lactating sow fed commercial diet for 24 days. 3 way cross bred 10 lactating sows with litters were alloted at farrowing pen. Crude protein and digestible energy were 14% and 3,467 kcal in diet, respectively. Average body weight of sow was 237.5kg. Feces with urine was produced 6.98kg per day by lactating sow ranging from 5.72 to 7.93kg. Manure solid was produced 2.75 kg per day ranging from 2.17 to 3.87kg and urine 4.23kg per day ranging from 3.55 to 4.06kg, respectively. Dry matter of feces and urine were 29.91 and 1.54%, respectively. Nitrogen and phosphorus contents in fresh feces were 0.86 and 0.30%, respectively. Nitrogen content in urine was 0.86, and phosphorus in urine was 0.47%. pH in manure was 6.73 and in urine was 8.38 respectively. Biochemical oxygen demand and suspended solid in urine were 5,196 and 308mg/$\ell$, respectively.

계분을 이용하기 위한 Thermoactinomyces sp. 균처리 (Treatment of Thermoactinomyces sp. to Application of Poultry Feces)

  • 최무영;이은
    • 한국미생물·생명공학회지
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    • 제18권5호
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    • pp.530-534
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    • 1990
  • 방선균의 한 종인 Thermoactinomuces sp. CH-53이 퇴비에서 분리되었다. 분리된 이 균은 pH 6.5-9.5, 수분 55-65의 무살균계분에서 왕성히 성장하며, 밀기울 배지에 포자를 충분히 착생시켜 시판의 배합사료에 1 비율로 첨가하여 닭에 급여해서 방선균의 생균수가$10^7-10^8$. cell/g에 도달하는 계분을 얻었으며, 처리 동안 악취성분이 소실되었다. 처리된 계분의 비료효과실험은 pot에서 Brassica rapa var. perviridis의 생육조사에서 다량시용에도 생육저해가 없었으며 질소함량으로 pot당 0.4g에 해당하는 시용구에서 최대생산을 보였다.

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볏짚과 계분(鷄糞)의 혼합물로 부터 Thermoactinomycetes vulgaris에 의한 생물비료(生物肥料)의 제조(製調) (Production of Biofertilizer from the Rice Straw Mixed with Hen Feces with Thermoactinomycetes vulgaris)

  • 최무영;강신정;이재성
    • Applied Biological Chemistry
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    • 제31권1호
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    • pp.100-105
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    • 1988
  • 계분(鷄糞)과 볏짚 혼합물을 Thermoactinomycetes vulgaris로 처리하므로써 악취가 나지 않고 작물(作物) 생육(生育)을 촉진시키는 생물비료(生物肥料)를 제조하였다. 비료(肥料) 제조(製調)의 처리조건은 온도 $50^{\circ}C\;pH\;8.0{\sim}8.5$, 수분함량(水分含量) 60% 였으며 발효(醱酵) 2일(日) 후에는 악취 성분으로 알려진 휘발성 지방산이 사라졌으며 결과적으로 계분(鷄糞)의 악취가 제거되었다. 제조(製調)된 비료(肥料)의 비료실험(肥效實驗)은 pot에서 Brassica rapa var. previdis의 생육(生育)을 조사(調査)함으로서 행하였다. 생물비료(生物肥料)는 황산암모니아, 건조계분(鷄糞), 평지깻묵 보다 작물생육(作物生育)을 촉진 시켰으며 다량(多量)의 시용(試用)으로도 생물(生物)의 생육(生育)저해가 작았다. 질소함량으로 pot당(當) 0.8g에 해당하는 생물비료(生物肥料)를 함유(含有)하는 시용구(試用區)에서 작물(作物)의 최대생산량(最大生産量)을 보였다.

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