• Title/Summary/Keyword: fecal examination

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A small-scale survey of intestinal parasite infections among children and adolescents in Legaspi city, the Philippines

  • Lee, Kyu-Jae;Ahn, Yung-Kyum;Yong, Tai-Soon
    • Parasites, Hosts and Diseases
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    • v.38 no.3
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    • pp.183-185
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    • 2000
  • To determine the status of infection caused by intestinal parasites among children and adolescents living in Legaspi city, the Philippines, we performed a small survey by fecal examination for helminth ova and protozoan cysts with formalin-ether concentration method. Of the 64 examinees, the infection rate was 78.1%. The infection rates of primary school children, preschool children and adolescents were 95.5%, 64.7% and 87.5%, respectively. The infection rate in urban areas was 56%, and 92.3% in rural areas. The infection rates were 51% with Trichuris trichiura, 40% with Ascaris lumbricoides, 23.4% with hookworm, 15.6% with Iodamoeba butschlii, 14.1% with Endolinax nana, 9.4% with Entamoeba coli and 7.8% with Giardia lamblia. There were 33 cases with multiple infection (51.6%). Mixed infection with more than 3 parasites was observed in 15 cases, all of them being children and adolescents living in rural areas. By this survey, it was conjectured that helminthic infection is prevalent among children and adolescents in Legaspi, Philippines. To determine the status of infection caused by intestinal parasites among children and adolescents living in Legaspi city, the Philippines, we performed a small survey by fecal examination for helminth ova and protozoan cysts with formalin-ether concentration method. Of the 64 examinees, the infection rate was 78.1%. The infection rates of primary school children, preschool children and adolescents were 95.5%, 64.7% and 87.5%, respectively. The infection rate in urban areas was 56%, and 92.3% in rural areas. The infection rates were 51% with Trichuris trichiura, 40% with Ascaris lumbricoides, 23.4% with hookworm, 15.6% with Iodamoeba butschlii, 14.1% with Endolinax nana, 9.4% with Entamoeba coli and 7.8% with Giardia lamblia. There were 33 cases with multiple infection (51.6%). Mixed infection with more than 3 parasites was observed in 15 cases, all of them being children and adolescents living in rural areas. By this survey, it was conjectured that helminthic infection is prevalent among children and adolescents in Legaspi, Philippines.

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A survey on the prevalence of parasites in Korean indigenous goats of Iksan-branch (익산지소 관내 재래산양의 내부 기생충 감염 실태 조사)

  • Koh Won-Seok;Lee Jae-Woog;Im Jeong-Cheol;Han Jae-Cheol;Lee Hee-Mun
    • Korean Journal of Veterinary Service
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    • v.28 no.2
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    • pp.107-112
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    • 2005
  • In order to monitor the parasites, 204 fecal samples were taken from Korean indigenous goats of Iksan-branch. Then identification of the parasites was determined by the fecal examination using the floatation and microscopical examination, respectively. The detection of rates was $91.2\%$, and mixed infection rates were single $38.7\%,\;double\;28.4\%,\;triple\;15.2\%,\;Quadraple\;6.9\%\;and\;Qunituple\;20.0\%$. The isolated were identified as Eimeria spp from 169 heads, Strongyloides papillosus from 56 heads, Ostertagia spp from 24 heads, Trichostrongylus spp from 22 heads, Moniezia expensa from 18 heads, Oesophagostomum spp from 17 heads, Bonostomum spp from 12 heads, Cooperia spp from 12 heads, Heamonchus spp from 8 heads and Capillaria spp from 2 heads.

Relations between the Micorfloral Composition and the Environmental Factors Affecting Korean Infants during Lactation (수유기 영아의 장내균총 조성과 환경인자와의 관계)

  • 진효상
    • Journal of Nutrition and Health
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    • v.32 no.1
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    • pp.67-74
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    • 1999
  • The relations between micorfloral compositions and environmental factors of 32 Korean infants were sought through statistical analysis after examinaton of fecal bacterial and questions to their mothers about feeding experiences at three different times for each subjects, about one week after birth, before weaning (2-3 months after birth), and after weaning 95-7 months after birth). The majority of mothers fed their infants cereal foods after the age of 4 months and began weaning with fruit juice and commercial weaning foods. Defection frequencies and fecal pH of infants decreased significantly during the examination period and 37.5% of total infants in fecal samples increased significantly during the examination period, which means that the kinds of bacterial genera increase with aging of infants. Frequencies of streptococci were significantly higher in infants fed delivered by Caesarian section than infants delived naturally. Frequencies of clostrida were significantly higher in infants fed with cerealfood before 4 months of age than after 5 months. The infants fed with probiotics showed significantly higher frequencies of veillonella at about 1 week old. They also showed significantly higher frequencies of clostridia before weaning than the infant fed with no probiotics, but significantly lower frequencise of C. perfringens before weaning. The infants fed with probiotics showed significantly higher number of streptococci at the age of about 1 week and significantly higher numbers of total aerobes before weaning, but significantly lower numbers of bacteroides after weaning than their counter parts. The fecla pH was directly proportional to the number of clostridia, klebsiella, and total aerobes at about 1 week after birth, to the number of E. coli before weaning, and to the number of streptococci and clostridia after weaning. Fecal pH had a negative relationship to the total number of anaerobes in 1-week-old infants. The infants that had diarrhea during lactation showed higher frequencies of bacteroides before weaning than those that didn't.

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BACTERIOLOGICAL STUDIES ON MARKET SEA FOODS 2. Sanitary Indicative Bacteria in Slices of Raw Fish (시판 수산식품에 대한 세균학적 연구 2. 생선회의 위생지표 세균에 관하여)

  • CHANG Dong-Suck;CHOE Wi-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.6 no.3_4
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    • pp.92-96
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    • 1973
  • This examination was carried out to evaluate the sanitary quality of slices of raw fish being served in the restaurants. Twenty five kinds of slices of raw fish collected from various restaurants in Busan Korea were examined during the period from March to May in 1971. As the evaluation factors of sanitary quality, the contents of sanitary indicative bacteria such as coliform group, fecal coliform, feral streptococci and enterococci and plate counts were determined. The results obtained are as in below: 1. The numbers of fecal streptococci and enterococci MPN were much greater than those of coliform group and fecal coliform. 2. The median value of coliform group MPN was 3,300 per 100 grams of the sample examined and those of enterococci was 5,400. The median value of plate counts was $1.8\times10^5$ per gram. 3. Fifty-two percent of the samples examined were exceeded fecal coliform MPN 930 per 100 grams. 4. As a sanitary indicative bacteria fecal coliform MPN was more reasonable than enterococci 5. The grade of restaurants was not correlated with the bacterial quality of the foods served. 6. No correlation between the numbers of sanitary indicative bacteria ana plate counts was observed.

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Clinicopathological Studies on the Subclinical Fascioliasis in the Korean Native Cows in Chonnam Area (한우개량단지 소의 간질병에 관한 임상적 연구)

  • 위성하;이정길
    • Korean Journal of Veterinary Service
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    • v.14 no.1
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    • pp.7-12
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    • 1991
  • Fecal samples were taken from 402 cows in Posung, Chonnam where was designated as a place for Korean native cattle breeding. Prevalence of intestinal parasitism were determined by the fecal examinations using the floatation and sedimintation procedures. 62.9% of the cows were found as positive cases with excretion of the eggs of Fasciola hepatica in the fecal specimens. Of those infected with F.hepatica 97 cows free of the pathogenic intestinal parasites were chosen for albendazole treatment. Albendazole tablets(10mg / kg) were administered to the cows twice at the interval of 4 weeks. Blood samples were collected via jugular vein prior to the first treatment. four weeks after the first treatment and four weeks after the second treatment respectively. At the same time fecal samples were collected for parasitological examination by sedimintation methods. The mean treatment count was 44 fluke egg per gram of feces, which compared with 27 EPG and 17 EPG four weeks after the first and second treatment, respectively. Most of the hematological values fluctuated within the normal range during the experiment. Eosinophil counts were high initially, decreased after the first treatment and there after remained steady.

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Evaluation of the Bacteriological Quality of a Shellfish-growing Area in Kamak Bay, Korea

  • Kwon, Ji-Young;Park, Kun-Ba-Wui;Song, Ki-Cheol;Lee, Hee-Jung;Park, Jeong-Heum;Kim, Jin-Do;Son, Kwang-Tae
    • Fisheries and Aquatic Sciences
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    • v.11 no.1
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    • pp.7-14
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    • 2008
  • The sanitary quality of a shellfish-growing area in Kamak Bay, Korea, was assessed through a bacteriological examination of seawater and oysters from January 2004 to December 2006. From seawater samples collected at 28 stations, the range of geometric mean (GM) and the estimated 90th percentile for most-probable-number (MPN) values of fecal coliforms were <1.8-2.4 MPN/100 mL and 1.8-6.0 MPN/100 mL, respectively. The observed fecal coliform GM and the estimated 90th percentile did not exceed the fecal coliform water quality standards of 14 MPN/100 mL and 43 MPN/100 mL. Therefore, the bacteriological quality of seawater at this shellfish-growing area met the Korean Shellfish Sanitation Program (KSSP) criteria for a growing area used for export. The range of the fecal coliform GM and the estimated 90th percentile MPN values of oyster samples were 19.2-160 MPN/100 g and 20.2-166.9 MPN/100 g, respectively, and the range of the viable cell count was 30-1900 CFU/g. Thus, the fecal coliform value for the oysters and the viable cell count were less than the current shellfish quality standards of 230 MPN/100 g and 50,000 CFU/g, respectively. The bacteriological quality of the oysters complied with the criteria for domestic use and export of shellfish.

Factors Predicting Fecal Occult Blood Testing among Residents of Bushehr, Iran, Based on the Health Belief Model

  • Dashdebi, Kamel Ghobadi;Noroozi, Azita;Tahmasebi, Rahim
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.sup3
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    • pp.17-22
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    • 2016
  • Colorectal cancer is a major cause of mortality worldwide. Fecal occult blood testing has proven a very effective screening tool for early detection and mortality reduction. The aim of this study was to determine predictors factors related to fecal occult blood testing using the Health Belief Model method among residents of Bushehr, Iran. A cross sectional study was performed on a sample of 600 men and women more than 50 years of age. The sample was selected by a convenience method from patients referred to public and private laboratories throughout the city. Each subject filled out a questionnaire which was designed and developed based on Health Belief Model constructs. Statistical analysis was conducted using ANOVA, T-test, chi-square test, and logistic regression. Fecal occult blood tests were performed on 179 (29.8%) out of 600 subjects, of which 95 patients (58.1%) did a periodic examination test and 84 patients (46.9%) had a doctor's advice for testing. According to the logistic regression model, the perceived barriers (P=0.0, Exp(B)= 0.3), perceived benefits (P<0.01, Exp(B)= 1.9) and self-efficacy (P<0.01, Exp(B)= 1.6) were predictive factors related to occult blood testing among subjects. The results showed that reducing people's perception of barriers to testing, increasing perceived benefits of screening, and reinforcing self efficacy can have major effect in increasing the rate of fecal occult blood screening for colorectal cancer prevention.

A novel kit for enrichment of fecal helminth eggs

  • Eunsol Lee;Seon-Ok Back;Young-Ju Lee;Jung-Won Ju;Hee-Il Lee;Myoung-Ro Lee
    • Parasites, Hosts and Diseases
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    • v.62 no.3
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    • pp.323-329
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    • 2024
  • We developed a new concentration kit, called the ParaEgg (PE), for easy detection trematode eggs from fecal samples in endemic areas of clonorchiasis and metagonimiasis in Korea. To create a standard of detection efficiency, 120 fecal samples were examined using the water-ether concentration method (WECM). The PE kit and Mini ParaSep (PS) kit were used to compare the detection sensitivity of 100 egg-positive and 20 egg-negative samples in WECM. Additionally, stool samples, which were intentionally spiked with 10, 20, and 30 Clonorchis sinensis eggs, were evaluated to assess the sensitivity in low-infection cases. The PE and PS kits showed detection rates of 100% and 92%, respectively, from 100 egg-positive samples in WECM. Meanwhile, eggs were detected in 3 (PE) and 2 (PS) out of 20 egg-negative samples in WECM. The PE kit detected the highest number of eggs per gram of feces (727 on average), followed by the WECM (524) and PS kit (432). In fecal samples that were intentionally spiked with 10, 20, and 30 C. sinensis eggs, PE only detected eggs 2 out of 5 samples in 10 eggs spiked (40%), and the detection rates were 80% and 100%, respectively. The PE kit enabled a more accurate identification of trematode eggs because of the clearance of small fecal debris in the microscopic field. In conclusion, the PE kit is obviously helpful to detect and identify trematode eggs in stool examinations especially in endemic areas of clonorchiasis and metagonimiasis.

A Survey on the Prevalence of Internal Parasites in Korean Native Goats and Sheep of Chonbuk Area (전북지방 산양과 명양의 내부기생충 실태조사)

  • 양홍지;서창섭;정재명;김종승
    • Korean Journal of Veterinary Service
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    • v.17 no.3
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    • pp.190-197
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    • 1994
  • In order to monitor the parasites, 303 fecal samples were taken from Korean native goats (n=279) and sheep(n=24) in Chonbuk area. The identification of the parasites were determimed by the fecal examination using the floatation or sedimentation methods and microscopical examination, respectively. The obtained results were summarized as follows ; 1. The detection rate of the parasites from Korean native goats and sheep was 98.2% and 83.3%, respectively. 2. In Korean native goat, mixed infection rate was single 7.5%(21 heads), double 47.3%(132 heads), triple 40.8%(114 heads), quadraple 5.0%(14 heads) and quintuple 2.5%(7 heads). 3. In sheep, mixed infection rate was single 12.5%(3 heads), double 45.8%(13 heads) and triple 16.6%(4 heads). 4. The parasites isolated were identified as Eimeria spp. from 283 heads, Oesophagostomum spp. from 96 heads, Ostertagia spp. from 97 heads, Strongylodes papillus from 97 heads, Heamonchs spp. from 73 heads, Trichostrongylus spp. from 42 heads, Moniezia expenza from 26 heads, Cooperia spp. from 16 heads, Nematodirus spp. from 9 heads, Bonostomum spp. from 9 heads, Paraphistomum spp. from 7 heads, and Fasciola hepatica and Capillaria spp. In 2 heads, in order.

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Cecocolic Intussusception Caused by Ancylostoma caninum Infection in a Dog

  • Ko, Hui-Yeon;Kim, Joonyoung;Geum, Migyeong;Suh, Guk-Hyun;Shin, SungShik;Kim, Ha-Jung
    • Journal of Veterinary Clinics
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    • v.37 no.2
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    • pp.106-108
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    • 2020
  • An eight-month-old, outdoor, intact male English Pointer dog weighing 23.5 kg presented to the hospital with signs of hematochezia, soft stools, and weight-loss. There were no remarkable findings on physical examination, complete blood count, serum biochemistry, electrolyte and gas analysis, and radiography. The serologic and Polymerase Chain Reaction (PCR) tests for canine parvovirus were negative. A fecal smear examination showed rod-shaped, sporeforming bacteria. Additionally, a fecal flotation test showed ova of Ancylostoma spp. The size of ova was 60 × 40 ㎛, and it was identified as Ancylostoma caninum using light microscopy. The PCR test indicated a Clostridial perfringens infection and the presence of C. perfringens alpha toxin. The diagnosis given was C. perfringens enterotoxicosis with ancylostomiasis. Treatment included antibiotics (metronidazole, trimethoprim-sulfamethoxazole) and anthelmintics (afoxolaner, milbemycin oxime). After two weeks, the clostridial infection resolved, but ancylostomiasis persisted for six weeks. The anthelmintic was changed to Drontalâ plus (praziquantel/pyrantel pamoate/febantel). After four weeks, there were no remarkable findings in the fecal samples, but the patient still presented with watery stools and hematochezia. Survey of abdominal ultrasound had performed, and a target-like sign with multiple rings was seen in the cecocolic region. The patient was diagnosed with A. caninum-induced cecocolic intussusception from the history and clinical signs. After a surgery, he recovered fully. This is the first clinical case report of Ancylostoma caninum parasitizing from the small intestine and causing an intussusception in the large intestine.