• Title/Summary/Keyword: fatty acid analysis

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Effects of Feeding Mugwort Powder on Meat Composition and Sensory Characteristics in Gilt (인진쑥 첨가 비육돈의 식육 성분 조성 및 관능적 특성에 미치는 영향)

  • Kim, Il-Suk;Jin, Sang-Keun;Kang, Suk-Nam
    • Food Science of Animal Resources
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    • v.29 no.1
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    • pp.68-74
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    • 2009
  • This study was conducted to determine the effects of mugwort powder on meat compositions (proximate analysis, meat cholesterol, fatty and amino acid composition) and sensory characteristics in finishing pigs ($LY{\times}D$). Each $20{\times}3$ pigs (75 kg) were randomly allotted to three treatments. Mugwort powder was added for 0 (C), 0.5 (T1), 1.0 (T2), or 1.5% (T3) in basal diet, respectively for 50 days. Moisture, protein, ash, and cholesterol were not significantly different between the samples, however, the compositions of fat in T2 and T3 were significantly lower than the control (p<0.05). The amino acid composition did not differ significantly in the samples. The total sum of saturated fatty acid (SFA) and unsaturated fatty acid (UFA) did not differ significantly in the samples. The compositions of linoleic acid, linolenic acid, essential fatty acid (EFA) and polyunsaturated fatty acid (PUFA) were lowest significantly in T3, however, monounsaturated fatty acid (MUFA) were highest in T3 (p<0.05). The fatty acid compositions of arachidonic acid, EFA and PUFA were highest significantly in T2 (p<0.05). There were no significant differences in the sensory characteristics of fresh and cooked meats in the samples.

Compositions of fatty acids and structural identification in human breast milk (모유 중 지방산 조성 및 구조 분석)

  • Ji-Woo, Yoo;Young-Soo, Keum;Nam Mi, Kang
    • Analytical Science and Technology
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    • v.35 no.6
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    • pp.229-236
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    • 2022
  • Lipidomic analyses of transient breast milk are far more limited than those of other dairy products. As a preliminary analysis of breast milk lipidomes, analytical methods for polar and nonpolar lipids from transient breast milk were developed, and detailed fatty acid profiles were determined in this study. The newly developed methods include solvent fractionation of phospholipids and acyl glycerol, one-pot derivatization to FAMEs and pyridylcarbinol esters, and instrumental analysis, including GC-FID and GC-MS. The results indicate that breast milk contains 16 major common fatty acids with 8-22 carbons. Additionally, 29 minor fatty acids were identified, including odd-numbered fatty acids and branched analogues with 11-23 carbons. Their detailed concentrations in different fractions were measured using the internal standard method. In addition to ordinary fatty acids, breast milk contains several branched fatty acids, including iso/anteiso acids with 15-18 carbons. Structural studies have been performed on selected minor fatty acids via chemical synthesis.

STUDIES ON THE SEPARATION & DETERMINATION OF CIS-TRANS FATTY ACIDS IN CIGARETTE SMOKE CONDENSATE BY GLASS CAPILLARY GAS CHROMATOGRAPHY (유리모세관칼럼에 의한 담배연기응축물중의 cis-trans형 지방산의 분리정량에 관한 연구)

  • Rhee, M.S.;Lee, U.C.;Kim, C.H.
    • Journal of the Korean Society of Tobacco Science
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    • v.7 no.1
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    • pp.67-74
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    • 1985
  • Cis-trans forms of fatty acids in cigarette smoke condensate were separated and characterized using SP 2340 glass capillary gas chromatography. The transforms of fatty acids, palmitoeladic , 1-vaccenic, eladic and linoeladic acid were identified. These components were not present only 3% of the total fatty acid fraction, but they showed a very low transfer rate.

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Green cabbage supplementation influences the gene expression and fatty acid levels of adipose tissue in Chinese Wanxi White geese

  • Bin Wang;Zhengquan Liu;Xingyong Chen;Cheng Zhang;Zhaoyu Geng
    • Animal Bioscience
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    • v.36 no.10
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    • pp.1558-1567
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    • 2023
  • Objective: Dietary green cabbage was evaluated for its impact on fatty acid synthetic ability in different adipose tissues during fattening of Wanxi White geese. Methods: A total of 256 Wanxi White geese at their 70 days were randomly allocated into 4 groups with 4 replicates and fed 0%, 15%, 30%, and 45% fresh green cabbage (relative to dry matter), respectively, in each group. Adipose tissues (subcutaneous and abdominal fat), liver and blood were collected from 4 birds in each replicate at their 70, 80, 90, and 100 days for fatty acid composition, relative gene expression and serum lipid analysis. Two-way or three-way analysis of variance was used for analysis. Results: The contents of palmitic acid (C16:0), palmitoleic acid (C16:1), linoleic acid (C18:2), and alpha-linolenic acid (C18:3) were feeding time dependently increased. The C16:0 and stearic acid (C18:0) were higher in abdominal fat, while C16:1, oleic acid (C18:1), and C18:2 were higher in subcutaneous fat. Geese fed 45% green cabbage exhibited highest level of C18:3. Geese fed green cabbage for 30 d exhibited higher level of C16:0 and C18:0 in abdominal fat, while geese fed 30% to 45% green cabbage exhibited higher C18:3 in subcutaneous fat. The expression of Acsl1 (p = 0.003) and Scd1 (p<0.0001) were decreased with green cabbage addition. Interaction between feeding time and adipose tissue affected elongation of long-chain fatty acids family member 6 (Elovl6), acyl-CoA synthetase longchain family member 1 (Acsl1), and stearoly-coA desaturase 1 (Scd1) gene expression levels (p = 0.013, p = 0.003, p = 0.005). Feeding time only affected serum lipid levels of free fatty acid and chylomicron. Higher contents of C16:0, C18:1, and C18:3 were associated with greater mRNA expression of Scd1 (p<0.0001), while higher level of C18:2 was associated with less mRNA expression of Scd1 (p<0.0001). Conclusion: Considering content of C18:2 and C18:3, 30% addition of green cabbage could be considered for fattening for 30 days in Wanxi White geese.

Crown Shape Control of Pinus koraiensis S. et Z. (VII) - The Influence of Thinning and Stem Pruning on Seed Component(The First Report) - (잣나무의 수형조절(VII) - 잣나무의 간벌과 수형조절이 종자의 성분에 미치는 영향(제1보) -)

  • Song, Jae-Mo;Shim, Tae-Heum;Yi, Jae-Seon
    • Journal of Forest and Environmental Science
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    • v.18 no.1
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    • pp.87-96
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    • 2001
  • In order to investigate the influence of increased seed production of Korean white pine(Pinus koraiensis S. et Z.), component of seeds, produced from trees in the thinned stand and the stem-pruned trees in the seed orchard, were analyzed. The results obtained were as follows: 1. General component analysis of seed There is no significant influence of thinning on the seed component in moisture content (thinned- 4.10%, unthinned- 3.74% ), ash (thinned- 2.95%, unthinned- 2.94%), crude lipid (thinned- 67.62%, unthinned- 71.94%), and crude protein (thinned- 17.27%, unthinned- 17.50%). There is no significant influence of stem-pruning on the seed component in moisture content (unpruned- 4.26%, 1m stem-pruned- 4.10%, and 2m stem-pruned- 3.99%), ash (unpruned- 2.08%, 1m stem-pruned- 2.09%, and 2m stem-pruned- 2.15%) crude lipid(unpruned- 68.59%, 1m stem-pruned- 69.52%, and 2m stem-pruned- 72.53%), and crude protein (unpruned- 18.13%, 1m stem-pruned- 17.96%, and 2m stem-pruned- 17.56%) 2. Fatty acid analysis Seeds of tree from thinned and unthinned stands contained two essential fatty acids, i.e., linoleic acid (18:2) (thinned- 54.92%, unthinned- 55.40%) and linolenic acid (18:3) (thinned- 0.19%, and unthinned- 0.23%). Over 94% of fatty acids consisted of linoleic acid (18:2)(55%), oleic acid (18:1) (32%), and palmitic acid (16:0)(7%). T-test showed difference of content at 1% significance level for palmitoleic acid (16:1), stearic acid (18:0) and 8.11, 14-eicosatrienoic acid (20:3) and at 5% for linolenic acid (18:3), but no significant difference for three major fatty acids mentioned above, between two types of trees. Thus thinning may give no influence in fatty acid content. Seeds of three types of trees contained two essential fatty acids, i.e., linoleic acid (18:2)(unpruned- 55.25%, 1m stem-pruned- 54.74%, and 2m stem-pruned- 55.00%), and linolenic acid (18:3)(0.21% for three kinds of trees). Linoleic acid (18:2)(55%), oleic acid (18:1) (32%), and palmitic acid (16:0)(6%) consisted of more than 93% of all fatty acid content. At 5% significance level in F-test, difference was observed in the content of palmitoleic acid (16:1) and 8.11, 14-eicosatrienoic acid (20:3), but not in three major types of fatty acids above mentioned. It is observed that stem-pruning gave no significant influence in fatty acid content in general.

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Oxidation Characteristics of Biodiesel and Its Blend Fuel I (바이오디젤 및 바이오디젤 혼합 연료의 산화 특성 I)

  • Jung, Chung-Sub;Dong, Jong-In
    • Applied Chemistry for Engineering
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    • v.18 no.3
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    • pp.284-290
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    • 2007
  • Biodiesel and its blend fuels from soybean oil were characterized for their oxidation to apply automobile fuel from the analysis of FAME (fatty acid methyl ester) and chemical properties. Biodiesel produced from soybean oil contained unsaturated fatty acids (> 85 wt%) such as oleic acid, linoleic acid, and linolenic acid. Especially, polyunsaturated fatty acids such as linoleic acid and linolenic acid containing active methyl radical were over 60 wt%. It is believed that linoleic acid and linolenic acid cause oxidation. Linoleic acid and linolenic acid during oxidation were major reactants, and compounds with the carbon number having around 36 (boiling point of about $500^{\circ}C$) were produced from those of radical autoxidation.

Quality Characteristics and Composition of the Longissimus Muscle from Entire and Castrate Elk in Korea

  • Kim, Sang-Woo;Kim, Kwan-Woo;Park, Seong-Bok;Kim, Myung-Jick;Yim, Dong-Gyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.5
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    • pp.709-715
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    • 2016
  • The objective of the research was to determine the chemical composition as well as the physicochemical properties of the longissimus muscle from Korean entire and castrate elk. Twelve elk stags were raised and fed on concentrate with ad libitum hay. All animals were equally divided into castrated and non-castrated (entire) males, and slaughtered at 5 year of age. It was found that entire elk, in comparison with castrate elk, had higher content of moisture and lower content of fat (p<0.05). Compared with entire males, the castrates had lower pH and shear force values (p<0.05). However, castrates had higher $L^*$, $a^*$, and $b^*$ values compared with entires (p<0.05). An analysis of the fatty acid profile revealed that the muscles of entire and castrate elk had the most abundant concentrations of the following fatty acids: palmitic acid (C16:0) of the saturated fatty acid, and oleic acid (C18:1n-9) of the unsaturated fatty acid. The entire elk contains higher proportions of linoleic acid (C18:3n6), eicosenoic acid (C20:1n9), and arachidonic acid (C20:4n6) (p<0.05). Cholesterol content in elk was not affected by castration. The predominant free amino acid was glutamic acid related to umami taste. It is apparent that the castrate animals carried higher content of histidine, isoleucine, and leucine than those of the entire group (p<0.05). In this study, it was concluded that venison quality of elk is affected by castration and these results can provide fundamental information for venison production.

Chemical Analysis of Pinus Leaves (소나무속 잎의 성분 분석)

  • 황병호;조재현;함승시;강하영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.6-9
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    • 2000
  • Chemical components such as vitamins, fatty acids, amino acids and rutins in the leaves of the Pinus densiflora Sieb. et Zucc., Pinus koraiensis Sieb. et Zucc., Pinus rigida Mill., Pinus thunbergii Parl. were analyzed. The vitamin A content in the P. densiflora and vitamin C content in the P. thunbergii were obtained more than the others. Oleic acid, linolenic acid, palmitic acid and lauric acid were the major fatty acids among 19 fatty acids detected in 4 kinds of pinus leaves and the only essential fatty acid of linolenic acid was also identified. The major amino acids were glutamic acid, leucine, arginine and aspartic acid among 17 kinds of amino acids detected in the pinus leaves and the amount of essential amino acids was 47.6% in P. densiflora. The highest amount of rutin was 10.34% in P. thunbergii.

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Diet of Chaetognaths Sagitta crassa and S. nagae in the Yellow Sea Inferred from Gut Content and Fatty Acid Analyses (위 내용물 및 지방산 구성을 통한 황해 모악류(Sagitta crassa와 S. nagae)의 먹이 섭식 특성)

  • Yoon, Hyunjin;Ko, Ah-Ra;Kang, Jung-Hoon;Choi, Joong Ki;Ju, Se-Jong
    • Ocean and Polar Research
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    • v.38 no.1
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    • pp.35-46
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    • 2016
  • To understand the diet of chaetognaths, the gut content and fatty acid trophic makers (FATMs) of Sagitta crassa and S. nagae, which are the most predominant species of chaetognath in the Yellow Sea, were analyzed. Gut contents of the two species examined by microscopic analysis revealed that copepods are the major components of the diet (> 70% of gut contents) and there was no significant changes in the gut contents of two species collected in spring and summer season. Although 16:0, 20:5(n-3) (Eicosapentaenoic acid) and 22:6(n-3) (Docosahexanoic acid), which are known as phytoplankton FA markers, were the most dominant among the fatty acids in both chaetognath species, the detection of copepod FA markers, 20:1(n-9) (Gadoleic acid) and 22:1(n-11) (Cetoleic acid), provided evidence that their food sources include copepods. These results suggest that S. crassa and S. nagae are carnivores and mainly feed on copepods in the Yellow Sea.

Fuel properties of biodiesel produced from beef-tallow and corn oil blends based on the variation in the fatty acid methyl ester composition

  • Woo, Duk Gam;Kim, Tae Han
    • Korean Journal of Agricultural Science
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    • v.46 no.4
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    • pp.941-953
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    • 2019
  • Biodiesels are being explored as a clean energy alternative to regular diesel, which causes pollution. In this study, the optimum conditions for producing biodiesel (BD) by combining beef tallow, an animal waste resource with a high saturated fatty acid content, and corn oil, a vegetable oil with a high unsaturated fatty acid content, were investigated, and the fuel properties were analyzed. Furthermore, Multivariate Analysis of Variance (MANOVA) was used to verify the optimum conditions for producing biodiesel. The influences of control factors, such as the oil blend ratio and methanol to oil molar ratio, on the fatty acid methyl ester and biodiesel production yield were investigated. As a result, the optimum condition for producing blended biodiesel was verified to be tallow to corn oil blend ratio of 7 : 3 (TACO7) and a methanol to oil molar ratio of 14 : 1. Moreover, the interaction between the oil blend ratio and the methanol to oil molar ratio has the most crucial effects on the production of oil blended biodiesel. In conclusion, the analysis results of the fuel properties of TACO7 BD satisfied the BD quality standard, and thus, the viability of BD blended with waste tallow as fuel was verified.