• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1755-1762
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• 2003

Two experiments were conducted to determine effects of particle size in total mixed ration (TMR) on performance of lactating cows. Three rumen cannulated Holstein cows were used in a $3{\times}3$ Latin square design for the metabolic experiment. The particle size of the diets was determined using the Penn State Particle Size Separator (PSPSS) and weighing the proportion of sample remaining on the top screen (19 mm diameter). The 3 treatments were short, medium or long diets (4.9, 24.2 and 27.8% of sample remaining on the top screen of the PSPSS, respectively). Nine farms in the Edmonton area were surveyed and the farms were placed into groups based on the particle size of the ration fed. The groups were short ${\leq}6%$, medium 7-12% and long ${\geq}13%$ of sample weight remaining on the top screen of the PSPSS. Dry matter intake was greater (p=0.07) for the medium diet than the long diet in the metabolic study and resulted in a higher (p=0.07) efficiency of milk production. On the commercial farms, a significantly (p=0.002) lower milk fat percentage was observed for the long diet compared to the short diet. The results of these studies confirm that forage particle size influences milk composition and milk fat was negatively correlated to TMR particle size.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 추계학술대회 및 정기총회
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• pp.142-142
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• 2003

• 대한의생명과학회지
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• 제12권4호
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• pp.319-327
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• 2006

This study aimed to determine whether troglitazone stimulates genes related to fatty acid ${\beta}$-oxidation, leading to modulation of white adipose tissue (WAT) metabolism in high fat diet-fed mice. Female C57BL/6J mice were randomly divided into two groups (n=10/group). After they received either a high fat diet or the same high fat diet supplemented with troglitazone for 4 weeks, the effects of troglitazone on gene expression and physiology of WAT were measured using Northern, histological and serological analyses. Administration of troglitazone induced the expression of genes involved in mitochondrial and peroxisomal fatty acid ${\beta}$-oxidation in mesenteric WAT. Troglitazone also significantly increased uncoupling protein 2 mRNA levels. The changes in WAT gene expression were accompanied by reductions in circulating levels of free fatty acids and triglycerides as well as glucose and insulin. Histological studies showed that troglitazone treatment decreased the average size of adipocytes in mesenteric WAT. These results suggest that troglitazone-stimulated WAT expression of genes associated with fatty acid ${\beta}$-oxidation regulates WAT metabolism of high fat diet-fed mice, contributing to improvement of insulin sensitivity.

• 한국한의학연구원논문집
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• 제9권1호
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• pp.145-156
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• 2003

Obesity is caused by imbalance of energy intake and expense. If energy intake is more than its expenditure, body does fat accumulation and affects body weight. It can be fetal disease although obesity is not disease in itself. Central regulatary system is affected by many neurotransmitters regulating .food intake in brain. Hypothalamus was known as one of food intake regulation in CNS. In order to investigate gene expression difference in hypothalamus by different nutrient, we used C57/BL6 control mouse and db-/db- mouse. They divided each of two group with mouse, and fed control diet and high-fat diet for 4 weeks. Each of control and high-fat diet contained 11.7% and 59.7% fat, respectively. Then we performed microarray assay with them. We compared among changed genes in hypothalamus region. In the results, we observed that increased genes were more than decreased genes. Although hypothalamus size of db-/db- mouse is smaller than that of C57/BL6, more genes were affected in db-/db- mouse. In this study, many genes are affected by nutrient in hypothalamus region.

• 한방재활의학과학회지
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• 제21권4호
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• pp.167-179
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• 2011

Objectives: In this study, we used with visceral fat area(VFA)/subcutaneous fat area(SFA) ratio(V/S ratio) and bioelectrical impedence analysis(BIA) for a comparative study between VFA measured from several abdominal computed tomography(CT) images and obesity indexes, such as body mass index(BM), waist circumference(WC), and waist-hip ratio(WHR). Methods: A group of 63 test subjects were gathered in the oriental medical hospital of Kyung-Won university. BIA for body composition and body size for obesity indexes were estimated to evaluate the obesity indexes. Pearson correlation coefficients and regression analysis were used to select useful obesity index. Results: The VFA-CT was significantly related to BMI, SFA, WC, hip circumference(HC), body fat mass(BFM), basal metabolic rate(BMR), and VFA-BIA. Especially, we found that the VFA-BIA and BMI were significantly correlated to VFA-CT. Conclusions: VFA-BIA index is an optimized index for diagnosis and evaluation of obesity. Finally, we found that the BMI is optimized to represent VFA.

• 대한융합한의학회지
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• 제5권2호
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• pp.11-16
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• 2023

Objectives: The purpose of this study was to investigate the anti-obesity effects of the aqueous extract of Schizandra chinensis (SC) in menopausal mice. Methods: To induce menopausal obesity, female mice were ovariectomized (OVX) and fed a high-fat diet (HFD; 60% fat, 28% carbohydrates, 14% protein) for 12 weeks. The mice were divided into 6 groups (n = 8): NOR (sham-operated and vehicle-treated), HFD+OVX (vehicle-treated), E2 (17-beta estradiol 50 ㎍/kg-treated), SC1 (1 mg/kg SC-treated), SC10 (10 mg/kg SC-treated), and SC100 (100 mg/kg SC-treated). Samples were orally administered for 6 weeks, after which all experimental mice were sacrificed. Body weight, feeding efficiency, white adipose tissue weight, adipocyte diameter, and fat vacuoles in liver were analyzed. Results: By treating with SC extract, the body weight and feeding efficiency of mice were significantly decreased. The weight of visceral fat tissues was decreased in the SC10 and SC100 groups. Histopathology showed that fat cell diameters of white adipose tissue were also decreased in the SC10 and SC100 groups. Additionally, SC extract regenerated the hepatocyte damage and decreased the size and number of follicular adipocytes Conclusion: In summary, these results suggest that SC has inhibitory effects against menopausal obesity. Schizandra chinensis may be a potential alternative for obesity among female menopausal diseases.

• 대한본초학회지
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• 제29권6호
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• pp.141-148
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• 2014

Objectives : Onion (AlliumcepaL.) is one of the richest sources of flavonoids in human diet. In this study, we studied the effects of onion peel water extract (OPE) on the blood lipid profiles and glucose in rats. Methods : The experimental groups were divided with 5 groups (n = 6) of SD rats: normal diet + distilled water (NC), high-fat diet + Quercetin (PC), high-fat diet + onion peel water extract 4 mg/kg (OPE-4), high-fat diet + onion peel water extract 20 mg/kg (OPE-20), high-fat diet + onion peel water extract 100 mg/kg (OPE-100). Results : The liver fat showed significantly lower weights and size in the OPE-100 group as compared with NC group (p<0.05), The epididymal fat and retroperitoneal fat showed significantly lower weights and sizes in the OPE-4 and OPE-20 group as compared with NC group (p<0.05). The serum levels of total cholesterol, LDL cholesterol and triglyceride were significantly lower in the OPE-4 and OPE-20 group as compared with NC group (p<0.05). The OPE-4 and OPE-20 group showed higher HDL cholesterol concentration than NC group (p<0.05). Atherogenic index of OPE-4 and OPE-20 group was significantly lower in as compared with NC group (p<0.05). The serum levels of glucose significantly lower in the OPE-20 group as compared with NC group (p<0.05). Conclusions : In these results, we suggests that onion peel water extracts supplementation can reduces the serum lipid components and improves the lipid metabolism in hyperlipidemic SD rat induced with a high-fat diet.

• 한국응용과학기술학회지
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• 제31권4호
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• pp.572-583
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• 2014

This study was carried out to determine the action mechanism of sulfur-grain larvae extract (SGE) on anti-obesity and the reduction of blood lipid level in high-fat diet induced obese model animals. Animals were classified into a normal diet group (NC, normal control), HFD (high-fat diet without SGE), HFD 15 (high-fat diet + oral administration of 15 mg of SGE extract per 100 g body weight) and HFD 30 (high-fat diet + oral administration of 30 mg of SGE extract per 100 g body weight). The body weight gain declined in HFD 15 and HFD 30 groups compared with the HFD group, even though the diet intake increased significantly. The weight of liver and adipose tissue increased significantly in HFD group compared with in the HFD 15 and HFD 30 groups. Triglyceride, total cholesterol, LDL-C and AI decreased in HFD 15 and HFD 30 groups compared with in the HFD group, but the contents of HDL-C increased significantly. Expression of SREPB-$1{\alpha}$, SREPB-2 mRNA in the liver was lower in the high-fat diet group compared with the HFD group, but the expression of LPL mRNA in adipose tissue and $PPAR{\alpha}$ increased significantly. Fat accumulation in the liver tissues and liver damage were greatly reduced in HFD 15 and HFD 30 groups compared with in the HFD group. The size of adipocytes became smaller in the HFD 15 and HFD 30 groups compared with HFD group. In conclusion, this research discovered for the first time that grain maggot has anti-obesity effects, by reducing the abdominal fat of obese model animals and lowering blood lipid level through the down-regulation of PPAR-$1{\alpha}$ and SREPB-2 mRNA and the up-regulation of PPAR-${\alpha}$ mRNA.

• 대한본초학회지
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• 제28권3호
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• pp.95-106
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• 2013

Objectives : The researcher investigated the anti-obesity effect of Alismatis Orientale Rhizoma(AP) water extract in mice fed a high fat diet and focused on the analysis of local area adipose tissue. Methods : Male ICR mice were divided into three groups, which were fed either a normal AIN diet, a 45% high fat diet (CT group), or a high fat diet and orally administration with a concentrations of 100 mg/kg (AP100 group) and 300 mg/kg body weight (AP300 group) for eight weeks. Results : As compared with CT group, AP100 group showed significant reductions in absolute weight of liver. As compared with CT group, AP100 group and AP300 group showed significant reductions in weight gain and relative weight of total fat. AST, triglyceride, total-cholesterol levels from the AP 100 group and 300 group were significantly lower than those of the CT, and ALT, LDL-cholesterol levels from the AP 100 group was significantly lower than those of the CT. But serum HDL-cholesterol levels from the AP 100 group and 300 group were significantly higher than those of the CT. And serum adiponectin levels from the AP 100 group was significantly higher than those of the CT. In result of real time PCR, all mRNA expression(PEPCK, G6Pase, PGC-$1{\alpha}$ and $ERR{\gamma}$) of two experimental groups were significantly decreased compared to those of CT group. The treatment with AP on local abdominal area made a fat cell size lessen on the fat tissue in the abdominal cavity and subcutaneous area. Conclusions : These results suggest that AP has an anti-obesity effect and the effect is mediated by inhibition of fat gain.

• 환경위생공학
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• 제12권2호
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• pp.13-21
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• 1997

The effect of upflow velocity on size distribution and activity of granular sludge was studied in laboratory-scale Expended Granular Sludge Bed(EGSB) reactor fed with non-fat dry milk and sucrose as sole carbon and energy source. High upflow velocity advanced size and activity of granular sludge by distribution and floatation of granular sludge. Therefore, the reactor operation of an apt upflow velocity was needed and an apt upflow velocity in this experimental was estimated to 1-10m/hr.