• Title/Summary/Keyword: fat content & distribution

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Sequence Characterization, Expression Profile, Chromosomal Localization and Polymorphism of the Porcine SMPX Gene

  • Guan, H.P.;Fan, B.;Li, K.;Zhu, M.J.;Yerle, M.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.7
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    • pp.931-937
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    • 2006
  • The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

Proposition and Application of a Dish-Based Target Pattern for Korean Adolescent Girls (여자 청소년 음식 기반 권장식사패턴의 제안과 이를 적용하여 작성한 식단의 평가)

  • Park, Mi Jin;Kim, Youngnam
    • Korean Journal of Community Nutrition
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    • v.20 no.2
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    • pp.87-95
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    • 2015
  • Objectives: Maintaining a balanced diet and thus health is crucial for adolescents, and the first step for balanced diet practice is meal planning. Adolescents, however, find it difficult to plan their meals. This study thus was set out to design an easier way of planning meals for adolescent girls. Methods: A dish-based target pattern for adolescent girls was tabulated, and validity of this was examined. Meal plan applying a dish-based target pattern was prepared by 150 female middle school students, and nutritional adequacies of those meal plans were examined. Validity and adequacy were tested by energy content, energy contribution ratio, nutrient adequacy ratio (NAR), probability of nutrient inadequacy, index of nutritional quality (INQ) calculation. Results: A dish-based target pattern with 11 dish groups was validated for nutritional adequacy. Though the NAR of calcium was 0.96, the INQ of calcium was 1.00. The average energy supply from the meal plans was 2,379 kcal, higher than the estimated energy requirement of a female middle school student, but the energy contribution ratio of carbohydrates, proteins, and fats were all adequate according to the acceptable macronutrient distribution range (AMDR). NAR of all nutrients examined were 1.0, except for calcium. The NAR and INQ of calcium were 0.87 and 0.75, respectively, and the meal plans at risk for calcium inadequacy was 19.30%. Conclusions: A dish-based target pattern proposed for adolescent girls was valid, but the meal plan prepared by female middle school students using this approach was high in energy and low in calcium supply. To cut down the energy supply from the meal plan, it is necessary to recommend dishes low in fat and use low fat cooking methods. To increase the calcium supply, it is important to recommend seaweed and legume group dishes with higher Ca INQ food items.

Distribution of Trypsin Indigestible Substrate(TI) in Seafoods and Its Changes during Processing - 1. Distribution and Post-mortem Changes of TI in Fish Muscle - (어패류(魚累類)의 Trypsin활성(活性) 저해물질(沮害物質) (TI)의 분포(分布)와 가공(加工) 중(中)의 변화(變化) - 1. 어육(魚肉) 중(中)의 TI의 분포(分布)와 어도저하(鮮度低下)에 따른 변화(變化) -)

  • Lee, Kang-Ho;Kim, Wha-Sim;Ryu, Hong-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.1
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    • pp.33-41
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    • 1984
  • To obtain the fundamental data on the nutritional value of protein for fresh meat, it was per- formed the distribution of Tl(trypsin indigestible substrates) and the apparent in vitro protein digestibility in 8 species of dark-fleshed fishes and8 species of white-fleshed fishes which were consumed in Korea popularly. It was also investigate the changes in VBN and TBA value during frozen storage at $-10^{\circ}C$on the purpose of assaying the antinutritional factors that affect on apparent in vitro protein digestibility or Tl forming. Tl content in dark-fleshed fishes were varied with their species, ranged from 0.02 to 0.17 mg/g. using the method by Hamerstrand, while that in white-fleshed fishes was almost same, ranged from 0.10 to 0.26 mg/g. For all the fresh fish samples, however, the apparent in vitro protein digestibility were showed the value from 83 to 83%. In comparison with the parts of pacific mackerel, viscera had the most abundant Tl content as much as 0.3m g/g, while a trace was noted for skin and dark muscle had more Tl content than ordinary muscle based on the method by Hamerstrand. The apparent in vitro protein digestibility for all samples was dropped but the changes of VBN and TBA were retested the similar tendency with the increasing Tl content during frozen storage at $-10^{\circ}C$. Therefore, it could be concluded that Tl contbnt and apparent in vitro protein digestibility were affected by its freshness and fat oxidation and that, especially, fat was assumed to play an important role on apparent in vitro protein digestibility.

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Comparison of The Taste Compounds of Wild and Cultured Eel, Puffer and Snake head (천연산 및 양식산 뱀장어, 복어, 가물치의 맛 성분에 대한 연구)

  • Kim, Hee-Yun;Shin, Jae-Wook;Sim, Gyu-Chang;Park, Hee-Ok;Kim, Hyun-Sook;Kim, Sang-Moo;Cho, Jae-Sun;Jang, Young-Mi
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1058-1067
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    • 2000
  • In this study, eel, puffer and snake head fish, which are widely taken and highly valued for processing into baked fish, soup or fish juice were studied by classifying them into wild fish and cultured fish, and evaluated in terms of taste. The samples were gathered in the area including Kimhae, Samchonpo and Haman in Kyungsangnam-do, Kangsu-Gu in Pusan and Bukcheju-Gun in Cheju-do. Proximate compositions, the content of nucleotides and their related compounds, total and free amino acids, organic acids and fatty acids of the fishes were analysed. Generally, as for the compositions, wild fish had a higher crude fat content and a lower moisture content as compared with cultured fish, while there was no great difference between them in terms of crude protein and ash contents. Nucleotides and their related compounds including ATP, ADP, AMP, IMP, HxR, Hx were detected, and the amounts of which were nearly the same with respect to the growth conditions. IMP content was high in all of the samples, while ATP content was extremely low. Total 17 amino acids were detected from the samples, and most of the samples had high contents of glutamic acid, aspartic acid, lysine, leucine, glycine, alanine and arginine, and low contents of cystein, isoleucine and methionine. As for the other amino acids, generally same level of contents were detected for each samples. In cases of eel and snake head fish, the wild fish had higher total amino acid contents in comparison with cultured fish. And the result to the contrary was obtained for the case of puffer. Sample fishes had nearly the same compose distribution of free amino acid though the contents of which were a little different according to the samples. Each sample had high contents of taurine, hydroxyproline, glutamic acid, glycine, cystathionine, ${\beta}-aminoisobutyric$ acid and lysine. Total organic acid contents of cultured fish was higher than that of wild fish. All fishes commonly had high contents of lactic acid, iso-valeric acid, oxalic acid and fumaric acid. High levels of $C_{16:0},\;C_{16:1},\;C_{18:1(cis)},\;C_{18:3},\;and\;C_{22:6}$ were shown in all of the samples and ${\omega}-3$ polyunsaturated fatty acid content of cultured fish was higher than that of wild fish, and as for the essential fatty acid, wild fish has higher content in comparison with cultured fish.

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Study on the Lubricity Characteristics of Bio-heavy Oil for Power Generation by Various feedstocks (다양한 원료에 따른 발전용 바이오중유의 윤활 특성 연구)

  • Kim, Jae-Kon;Jang, Eun-Jung;Jeon, Cheol-Hwan;Hwang, In-Ha;Na, Byung-Ki
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.985-994
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    • 2018
  • Bio-heavy oil for power generation is a product made by mixing animal fat, vegetable oil and fatty acid methyl ester or its residues and is being used as steam heavy fuel(B-C) for power generation in Korea. However, if the fuel supply system of the fuel pump, the flow pump, the injector, etc., which is transferred to the boiler of the generator due to the composition of the raw material of the bio-heavy oi, causes abrasive wear, it can cause serious damage. Therefore, this study evaluates the fuel characteristics and lubricity properties of various raw materials of bio-heavy oil for power generation, and suggests fuel composition of biofuel for power generation to reduce frictional wear of generator. The average value of lubricity (HFRR abrasion) for bio-heavy oil feedstocks for power generation is $137{\mu}m$, and it varies from $60{\mu}m$ to $214{\mu}m$ depending on the raw materials. The order of lubricity is Oleo pitch> BD pitch> CNSL> Animal fat> RBDPO> PAO> Dark oil> Food waste oil. The average lubricity for the five bio-heavy oil samples is $151{\mu}m$ and the distribution is $101{\mu}m$ to $185{\mu}m$. The order of lubricity is Fuel 1> Fuel 3> Fuel 4> Fuel 2> Fuel 5. Bio-heavy oil samples (average $151{\mu}m$) show lower lubricity than heavy oil C ($128{\mu}m$). It is believed that bio-heavy oil for power generation is composed of fatty acid material, which is lower in paraffin and aromatics content than heavy oil(B-C) and has a low viscosity and high acid value, resulting in inhibition of the formation of lubricating film by acidic component. Therefore, in order to reduce friction and abrasion, it is expected to increase the lubrication of fuel when it contains more than 60% Oleo pitch and BD pitch as raw materials of bio-heavy oil for power generation.

Green Tea Extract is an Effective Antioxidant for Retarding Rancidity of Yukwa (Rice Snacks) Fried in Soybean and Rice Bran Oils

  • Park, Jae-Hee;Kim, Chang-Soon
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.255-260
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    • 2002
  • Yukwa is a popular Korean traditional fried rice snack. The high fat content and porous structure of Yukwa cause it to rapidly become rancid, presenting difficult challenges for commercial distribution. In this study, an-tioxidant activities of green tea extracts (GTE) were evaluated in Yukwa fried in soybean oil (SBO), rice bran oil (RBO) and winterized rice bran oil (WRBO) during storage at 4$0^{\circ}C$ for 12 weeks. Lipid oxidation of Yukwa was determined by acid value (AV), peroxide value (POV), p-anisidine value(AnV), totox value and sensory evaluation. The addition of GTE to the oils reduced the increases in AV, POV, AnV, and totox. Totox increased most vapidly in Yukwa fried in SBO, fellowed by RBO>WRBO>SBO+200 ppm GTE>RBO+200 ppm GTE > WRBO + 200 ppm GTE (p<0.05). Sensory evaluation revealed that the addition of 200 ppm GTE delays rancidity in Yukwa by 7~8 weeks; providing compelling evidence that GTE is an effective antioxidant for Yukwa.

Quality Inspection to the Ginseng Saponins in Commercial Ginseng-extracts (인삼(人蔘)사포닌을 중심(中心)한 인삼정(人蔘精)의 품질조사(品質調査))

  • Cho, Kyu-Seong;Kim, Hai-Jung;Joo, Hyun-Kyu
    • Korean Journal of Pharmacognosy
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    • v.12 no.4
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    • pp.185-189
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    • 1981
  • The quality inspection was made on commercial Korean ginseng extract (Company A, B, C, D, E, F, G and H), particularly on samples available in Seoul market area. The results were as follews: 1) Among the ginseng extract products out of eight different manufactures, the moisture content of D company's product showed 46.5%, and other companys' met with the moisture standard for ginseng extracts. And protein, fat, ash, fiber and total sugar were about $9.87{\sim}21.07%,\;0.46{\sim}1.62%,\;6.55{\sim}7.88%,\;0{\sim}0.15%\;and\;58.58{\sim}76.74%$, respectively. And residue of D and F company products showed 3.25% and 3.61% which exceed the standard, and other company products met with residue test specifications. 2) The contents of ginseng saponins were 16.16% and 13.12%, respectively, for the C and H company products. However, other company's showed below 9%. By fractional distribution of ginseng saponins, it is supposed to be white ginseng and lateral ginseng that were mostly used as raw materials for ginseng extract manufacturing.

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Effect of Different Tumbling Marination Methods and Time on the Water Status and Protein Properties of Prepared Pork Chops

  • Gao, Tian;Li, Jiaolong;Zhang, Lin;Jiang, Yun;Yin, Maowen;Liu, Yang;Gao, Feng;Zhou, Guanghong
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.7
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    • pp.1020-1027
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    • 2015
  • The combined effect of tumbling marination methods (vacuum continuous tumbling marination, CT; vacuum intermittent tumbling marination, IT) and effective tumbling time (4, 6, 8, and 10 h) on the water status and protein properties of prepared pork chops was investigated. Results showed that regardless of tumbling time, CT method significantly decreased the muscle fiber diameter (MD) and significantly increased the total moisture content, product yield, salt soluble proteins (SSP) solubility, immobilized water component (p<0.05) compared with IT method. With the effective tumbling time increased from 4 h to 10 h, the fat content and the MD were significantly decreased (p<0.05), whereas the SSP solubility of prepared pork chops increased firstly and then decreased. Besides, an interactive effect between CT method and effective tumbling time was also observed for the chemical composition and proportion of immobilized water (p<0.05). These results demonstrated that CT method of 8 h was the most beneficial for improving the muscle structure and water distribution status, increasing the water-binding capacity and accelerating the marinade efficiency of pork chops; and thus, it should be chosen as the most optimal treatment method for the processing production of prepared pork chops.

Distribution of Trypsin Indigestible Substrate (TI) in Seafoods and Its Changes during Processing 2. Changes in TI and In Vitro Apparent Digestibility of Boiled and Dried Anchovy during Processing and Storage (어패류의 Trypsin활성 저해물질의 분포와 가공중의 변화 2. 자건멸치 가공저장중의 Trypsin활성 저해물질과 In Vitro Apparent Digestibility의 변화)

  • LEE Kang-Ho;JO Jin-Ho;RYU Hong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.101-108
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    • 1984
  • In order to study the optimal conditions of processing and storage for boiled and dried anchovy (Engraulis japonica) with high protein digestibility, the contents of trypsin indigestible substrate (TI) and in vitro apparent protein digestibility were determined. Peroxide value (PoV), TBA number and nonenzymatic brown pigments, that accounted for important antinutritional factors, were also measured and confirmed the relationship between those factors and formation of TI or in vitro protein apparent digestibility. The results were as follows; Samples boiled for 5 minutes showed the lower content of TI than the other samples boiled for 0.5 min. or 1 min. Hot air dried products had a lower TI content in comparison with the other dried ones such as sun dried or freeze dried products. It was revealed that the lower temperature ($8{\pm}1^{\circ}C$) did not affect to a great degree of forming TI and falling in vitro digestibility comparing to high temperature ($26{\pm}1^{\circ}C$) during storage. The lowest TI content (0.173 mg/g solid) was noted in the samples for 5 minutes and then sun drying after 56 days storage at $9{\pm}1^{\circ}C$. A rapid decrease of in vitro protein digestibility occurred within 0.5 min. of boiling and showed the value $85.3\%$. Freeze dried samples possessed the highest in vitro protein digestibility ($85.9\%$), when compared to sun dried or hot air dried products. Fat oxidation and nonenzymatic browning were proceeded with the various boiling times, drying methods and storing temperatures. It was noted that boiling for 5 minutes and freeze drying accelerate the fat oxidation significantly. More nonenzymatic brown pigments was developed in samples boiled for shorter time (0.5 min.) and that stored at high temperature ($26{\pm}1^{\circ}C$) than the other products. Therefore, fat oxidation and nonenzymatic browning assumed to be a major inhibitory reaction in enzyme digestion and those might be an important role in forming TI in boiled and dried anchovy products during processing and storage.

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A Study on Comparative Analysis of Food Characteristics of Sea Bream and Similar Species (도미와 유사어종의 식품학적 특성비교 분석에 관한 연구)

  • Chung, Ji-Yong;Jung, Young Mi
    • Culinary science and hospitality research
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    • v.23 no.2
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    • pp.159-168
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    • 2017
  • This study was conducted to compare the food quality of domesticated species. Consumers surveyed for safe food intake and proper culture of food distribution. The results of the comparison study are as follows. Muscle moisture content, protein content, and fat content. K, P, and C showed relatively high values in the muscle of the sea bream. Fe showed low contents. As a result of measuring heavy metal component, Cd was not detected in sea bream and mullet, but $0.01{\pm}0.00mg/kg$ was detected in red mine. Other heavy metals were below the reference value or were not detected. Electrophoresis results showed that the band appeared at in red minefish. In the case of sea bream and swordfish, no distinctive features of the band were shown. In the case of sea bream, there was little difference in food science between the similar fish species and the red sea bream fish, but price was different. An environment should be created for consumers to buy the right ingredients at the price they want. It is necessary to educate consumers about food ingredients immediately.