• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.593-598
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• 2008

Differential display RT-PCR was used for screening the differentially expressed specific genes by Rhus verniciflua extract treatment to U937 cell, human leukemic monocyte. As a result, 19 clones differentially expressed were detected. Among the detected clones, one clone was confirmed to be over-expressed by R. verniciflua extract treatment in Northern blot analysis. Nucleotide sequence of the clone showed 100% homology with H2A histone family member Z gene. Therefore, it is concluded that the treatment of R. verniciflua extract to U937 cell specifically induces the expression of H2A.Z gene but its role should be elucidated by future works.

• Journal of the Korea Safety Management & Science
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• v.8 no.1
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• pp.85-96
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• 2006

The oriental life change unit(LCU) models differ from the western LCU model in human behavior, life style, ideas, culture, and psychology factors. Especially, the cause and effect models by behavior scientific approach method have provided the accident proneness concept through the LCU factors. Therefore this paper describes the analytical results of the oriental LCU model and western LCU model. Especially, the death of parents and death of close friend in Korean LCU model feel more acute stress than the western factors. In the case of Korean LCU models between the unmarried and married workers living in the middle area of Korea, death of parents, change in family member's health, change to different line of work, and change in work hours and condition are significant at the significance level of 5%. The priority of life change factors in these models is approved in the order of death of parents, death of spouse, death of close family member, and death of close friend. Finally, the result of this paper can be easily used in order to minimize the industrial accidents by the job stress with the worker and safety manager in real fields.

• Archives of Pharmacal Research
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• v.22 no.5
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• pp.454-458
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• 1999

TR8 is a recently identified member of the tumor necrosis factor (TNF) receptor superfamily. TR8 seems to play important roles in bone metabolism as stimulation of this receptor with its ligand, TL8 or osteoclast differentiation factor (ODF), induced the differentiation and activation of osteoclasts. Despite its important biological functions, the biochemcial events ensuing form TR8 activation have not been revealed in detail. Most of TNF receptor family proteins provoke the activation of the NF-$textsc{k}$B transcription factor. In the present study, we examined the signaling potential of TR8 to induce NF-B activation. When overexpressed in a human embryonic kidney cell line by transient transfection, TR8 caused a strong activation of NF-$textsc{k}$B, which was further increased upon stimulation with TL8. The TR8-induced NF-B activation was abrogated by the co-expression of the TRAF6 mutnat lacking the Ring and zinc finger domains and that of the kinase-inactive mutant NIK. Taken together, our study suggests that the presence of intact TRAF6 and the kiase activity of NIK may be essential for TR8 to induce NF-$textsc{k}$B activation.

• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.73-81
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• 1985

We have previously isolated OsMADS4 gene that is a member of the class B MADS box genes from rice. In this study, another member of the class B MADS box genes was isolated from rice flower by the yeast two-hybrid screening method using OsMADS4 as bait. RNA blot analyses revealed that the clone, OsMADS16, was expressed in the second and third whorls, whereas the OsMADS4 transcripts were present in the second, third, and fourth whorls. These expression patterns of the OsMADS16 and OsMADS4 genes are very similar with those of AP3 and PI, the class B genes of Arabidopsis, respectively. In the yeast two-hybrid system, OsMADS4 interacted only with OsMADS16 among several rice MADS genes investigated, suggesting that OsMADS4 and OsMADS16 function as a heterodimer in specifying sepal and petal identities. We have also isolated OsMADS6 gene using OsMADS1 as a probe. Both are members of the AGL2 MADS family. Various MADS genes that encode for protein-protein interaction partners of the OsMADS6 protein were isolated by the yeast two-hybrid screening method. A majority of these genes belong to the AGL2 family. Sequence Homology, expression pattern, and ectopic expression phenotypes indicated that one of the interaction partners, OsMADS14, appears to be homologous to API, the class A MADS gene of Arabidopsis.

• Biomolecules & Therapeutics
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• v.9 no.2
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• pp.79-87
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• 2001

Heat shock proteins serve as chaperone by preventing the aggregation of denatured proteins and promote survival of pathogens in harsh environments. In this study, heat shock gene encoding a 84-kDa (p84) protein, which is one of the three major heat shock proteins in S. pneumoniae, was cloned and characterized. PCR with a forward primer derived from N-terminal amino acid sequence of the p84 and a reverse primer derived from the conserved second ATP-binding region of Clp family was used for amplification of the gene encoding the p84 and subsequently the PCR product was used for sequence determination. Sequence analysis of the p84 gene demonstrated that it is a member of ClpL. The deduced amino acid sequence of pneumococcal ClpL shows homology with other members of the Clp family, and particularly, even in variable leader region, with bovine Clp-like protein and L. lactis ClpL. S. pneumoniae clpL is the smallest clop member (701 amono acids) containing the two conserved ATP-binding regions, and hydrophilic N-terminal variable region of pneu-mococcal Clp ATPase is much shorter than any known Clp ATPases. Histidine tagged ClpL was overexpressed and purified from E. coli. Immunoblot analysis employing antisera raised against pneumococcus p84 demonstrated no cross-reactivity with Clp analog in Eschericha coli, Staphylococcus aureus and human HeLa cells. Preimmunization of mice with ClpL extended mice life partially but did not protect them from death.

• YAKHAK HOEJI
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• v.56 no.6
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• pp.352-357
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• 2012

FGFR3 is a member of the fibroblast growth factor receptor family which interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Accumulated evidence suggests that aberrant regulation of FGFR3 and genetic alterations are implicated in the development and progression of various cancers. Despite a high incidence of FGFR3 over-expression, no such investigation has been performed in hepatocellular carcinoma. Thus, we investigated genetic alterations of the FGFR3 gene in 73 cases of hepatocellular carcinoma by single-strand conformational polymorphism (SSCP) and sequencing. One silent mutation (A369A) was found in the extracellular domain of FGFR3, and one genetic alteration in the immunoglobulin-like III domain of FGFR3 appeared to be polymorphism. Taken together, we concluded that over-expression of FGFR3 in hepatocellular carcinoma is not associated with genetic alterations of FGFR3 gene, and we suggest that there could be another underlying mechanism of aberrant FGFR3 expression in hepatocellular carcinoma.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.543-551
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• 2001

The vascular endothelial growth factor (VEGF), or VEGF-A, is intimately involved in both physiological and pathological forms of angiogenesis. VEGF-A is now recognized as the founding member of a family of growth factors that has expanded to include VEGF-B, VEGF-C, VEGF-D, VEGF-E, and placental growth factor (PIGF). This family of cytokines binds differentially to at least three receptor tyrosine kinases, however, the extent to which family members other than VEGF-A contribute to physiological and pathological angiogenesis remains unclear. Issues that are of relevance include uncertainty regarding the consequences of signaling through VEGF - RI in particular, and the ability of some family members to heterodimerize, leading to the possibility ofheterodimeric receptor complexes. Structural characterization is one approach that can be used to address these issues, however, the vast majority of previous structure-function studies have only focused on VEGF-A. While these studies may provide some clues regarding the structural basis of the interaction of other family members with their receptors, studies using the ligands themselves are clearly required if highly specific interactions are to be revealed. With the recent progress toward refolding and purifying substantial' quantities of other VEGF family members, such structural studies are now possible. Here, these ~ssues are addressed with a particular emphasis on VEGF-B and its receptors.

• Journal of the Korean housing association
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• v.16 no.1
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• pp.37-45
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• 2005

The resident committee is a main board of the multi-family housing communities, which discuss and decide important matters regarding the multi-family housing management. So, the purpose of this study was to provide critical informations in order to revitalize the resident committee of multi-family housing. The research was conducted by surveys and interviews with residents and resident committee members. The major results were as follows : The resident committee members indicated that primary reason for them to volunteer as a committee member was because of the encouragement and recommendation of their neighbors. They were willing to be members of the resident committee but they were displeased by the lack of participation and training of some of the committee members. Resident committee members also pointed out that they had difficulties in fulfilling their roles. For one thing, they were not paid for their participation. Another problem was that there were several committee members who did not have knowledge of related laws and they gave themselves a negative evaluation, which told that they had lack of multi-family housing community management skills and knowledge. Training and education of the committee was essential in areas such as budget management, auditing, and damage control.

• Journal of the Korean Institute of Rural Architecture
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• v.18 no.1
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• pp.29-37
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• 2016

This research is studied changes of outdoor lavatories in use of detached houses in rural areas. Out of 40 houses studied in a 1995 survey, 23 houses were performed again in 2015. Number of lavatories installed in indoor and outdoor both in 1995 are more than in 2015. However, number of indoor lavatories in 2015 are more because outdoor lavatories were demolished. Among 23 houses, 15 outdoor lavatories have been installed in 13 houses. Out of these 15 lavatories, eight have been used continuously from 1995 through 2015. Currently four are used for other purposes, and five have demolished. Outdoor lavatories tend to be built in the rear of the housing site. Those located in rear tend to be demolished rather than in front side because of decreasing in use. In rural areas, outdoor lavatories not only serve as spaces to fulfill basic human needs, but are used to store agricultural tools. Changes of family composition and member also exert an influence. When family members were decreased, outdoor lavatories tended to be demolished. However, although family members were decreased, the reason that outdoor lavatories have been used continually is for exterior work or farming and temporarily increasing family members in holiday and family events. The more family members used, the more outdoor lavatories are managed well. The more houses where the aged reside in, the more outdoor lavatories are not improved.

• International Journal of Human Ecology
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• v.1 no.1
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• pp.1-14
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• 2000

Parental satisfaction is the foundation of a happy family. It is a key factor in overall life satisfaction and also a critical variable in the development of children, and thus an education program aimed at parental satisfaction improvement is needed. This study attempts to determine basic elements of parental satisfaction that could enable better parental education programs. To accomplish this, mother's parental satisfaction and factors related to it were examined. The subjects were 641 mothers of primary school and middle school students aged 10, 12, and 14 residing in Seoul, Pohang and Kwangyang. Structured self-administered questionnaires were used to ascertain mothers' parental role satisfaction, mothers' role values, children related variables, mother related variables, father related variables, and home environment variables. The findings indicate that the variables related to parental satisfaction are multi-dimensional, that mothers' parental satisfaction can be improved by other family member's effort, and that a family's external characteristics are less important than its internal characteristics. These results imply that it is essential to include the characteristics of the family system when designing parental education programs. Furthermore, the role of specific family members, namely the husband, should be expanded in more supportive ways in Korean families to improve mothers parental satisfaction.