• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3289-3294
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• 2016

Naringin, a bioflavonoid found in Citrus seeds, inhibits proliferation of cancer cells. The objectives of this study were to investigate the mode and mechanism(s) of hepatocellular carcinoma HepG2 cell death induced by naringin. The cytotoxicity of naringin towards HepG2 cells proved dose-dependent, measured by MTT assay. Naringin-treated HepG2 cells underwent apoptosis also in a concentration related manner, determined by annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) employing flow cytometry. Mitochondrial transmembrane potential (MTP) measured using 3,3'-dihexyloxacarbocyanine iodide ($DiOC_6$) and flow cytometer was reduced concentration-dependently, which indicated influence on the mitochondrial signaling pathway. Caspase-3, -8 and -9 activities were enhanced as evidenced by colorimetric detection of para-nitroaniline tagged with a substrate for each caspase. Thus, the extrinsic and intrinsic pathways were linked in human naringin-treated HepG2 cell apoptosis. The expression levels of pro-apoptotic Bax and Bak proteins were increased whereas that of the anti-apoptotic Bcl-xL protein was decreased, confirming the involvement of the mitochondrial pathway by immunoblotting. There was an increased expression of truncated Bid (tBid), which indicated caspase-8 proteolysis activity in Bid cleavage as its substrate in the extrinsic pathway. In conclusion, naringin induces human hepatocellular carcinoma HepG2 cell apoptosis via mitochondria-mediated activation of caspase-9 and caspase-8-mediated proteolysis of Bid. Naringin anticancer activity warrants further investigation for application in medical treatment.

• 대한기계학회논문집A
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• 제25권10호
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• pp.1551-1558
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• 2001

Recently, a number of underground pipelines have been drastically increased. The integrity of these buried pipelines, especially gas transmitting pipelines, is of importance due to an explosive characteristic of natural gas. The third party damage is known as one of the most critical factor which causes fatal accidents. For this reason, a number of systems detecting third party damage are under development. The major concern in the development of third party damage detection system is to transmit vibration signals out of accelerometer to signal conditioner and data acquisition system without any interference caused by noise. The objective of this paper is to develope a fiber optic accelerometer applicable to third party damage detection system. A fiber optic accelerometer was developed by use of combining principles of one degree of freedom vibration model and an extrinsic Fabry-Perot interferometer. The developed fiber optic accelerometer was designed to perform with a sensitivity of 0.06mVg, a frequency range of less than 6kHz and an amplitude range of -200g to 200g. The developed, accelerometer was compared with a piezoelectric accelerometer and calibrated. In order to verify the developed accelerometer, the field experiment was performed. From the field experiment, vibration signals and the location of impact were successfully detected. The developed accelerometer is expected to be used for the third party damage detection system which requires long distance transmission of signals.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2008년도 하계종합학술대회
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• pp.161-162
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• 2008

In this paper we design an irregular low-density parity-check (LDPC) code for a multi-input multi-output (MIMO) system. The considered MIMO system is minimum mean square error soft-interference cancellation (MMSE-SIC) detector. The MMSE-SIC detector and the LDPC decoder exchange soft information and consist a turbo iterative detection and decoding receiver. Extrinsic information transfer (EXIT) charts are used to obtain the edge degree distribution of the irregular LDPC code which is optimized for the input-output transfer chart of the MMSE-SIC detector. It is shown that the performance of the designed LDPC code is much better than that of conventional LDPC code optimized for the AWGN channel.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2003년도 춘계학술발표대회 논문집
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• pp.22-26
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• 2003

EFPI (extrinsic Fabry-Perot interferometer) 센서를 이용해 복합재료의 파손 신호를 취득하기 위해서는 파손 신호에 비해 상대적으로 낮은 주파수의 열적, 기계적 정적 변형에 의해 발생하는 위상 변화를 보상해 주는 기술이 필요하다. 또한 센서의 민감도를 최적화하기 위해 출력 신호의 위상을 Quadrature 지점에 유지시켜야 한다. 본 논문에서는 EFPI 센서 시스템의 출력 신호위상을 일정하게 유지시킬 수 있는 안정화 제어 시스템을 개발하였다. 안정화 제어 시스템은 광대역 파장 레이저 광원, 가변 F-P (Fabry-Perot) 필터 그리고 필터를 제어한 수 있는 전자 회로시스템으로 구성하였다. 개발된 시스템의 위상 제어 성능을 평가하기 위해 복합재료 시편의 인장 실험을 수행하여 인장 변형에 의해 발생하는 위상 변화를 개발된 시스템을 이용해 Quadrature 지점에 일정하게 유지할 수 있음을 보였다. 또한 연필심 파손 실험을 통해 개발된 시스템이 파손 신호를 잘 취득할 수 있음을 확인하였다.

• Nuclear Engineering and Technology
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• 제55권8호
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• pp.2873-2878
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• 2023

The purpose of this study is to perform radiation monitoring by acquiring gamma images and real-time optical images for ^99mTc vial source using charge couple device (CCD) cameras equipped with the proposed compact gamma camera. The compact gamma camera measures 86×65×78.5 mm³ and weighs 934 g. It is equipped with a metal 3D printed diverging collimator manufactured in a 45 field of view (FOV) to detect the location of the source. The circuit's system uses system-on-chip (SoC) and field-programmable-gate-array (FPGA) to establish a good connection between hardware and software. In detection modules, the photodetector (multi-pixel photon counters) is tiled at 8×8 to expand the activation area and improve sensitivity. The gadolinium aluminium gallium garnet (GAGG) measuring 0.5×0.5×3.5 mm³ was arranged in 38×38 arrays. Intrinsic and extrinsic performance tests such as energy spectrum, uniformity, and system sensitivity for other radioisotopes, and sensitivity evaluation at edges within FOV were conducted. The compact gamma camera can be mounted on unmanned equipment such as drones and robots that require miniaturization and light weight, so a wide range of applications in various fields are possible.

• 방송공학회논문지
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• 제24권6호
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• pp.919-927
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• 2019

본 논문에서는 입체 영상을 획득하기 위한 정밀 카메라 캘리브레이션(calibration) 기법을 제안한다. 일반적인 카메라 캘리브레이션 기법은 체커보드 구조의 목적 패턴을 이용하여 수행한다. 체커보드 패턴은 사전에 인지된 격자구조를 활용할 수 있으며, 체커보드 코너점을 통해 특징점 매칭을 용이하게 수행할 수 있음에 따라 2차원 영상 픽셀 지점과 3차원 공간상의 관계를 정확히 추정할 수 있다. 특징점 매칭을 통해 카메라 파라미터를 추정하므로 정밀한 카메라 캘리브레이션을 위해선 영상 평면내의 정확한 체커보드 코너 검출이 필요하다. 따라서 본 논문은 정확한 체커보드 코너 검출을 통해 정밀한 카메라 캘리브레이션을 수행하는 기법을 제안한다. 정확한 코너를 검출하기 위해 1-D 가우시안 필터링을 활용하여 코너 후보군들을 검출한 후 코너 정제(refinement) 과정을 통해 이상치(outlier)들을 제거하며 영상내의 부분 픽셀(sub-pixel) 단위의 정확한 코너를 검출한다. 제안한 기법을 검증하기 위해 카메라 내부 파라미터를 추정 결과를 판단하는 재투사 오차(reprojection error)를 확인하며, 카메라 위치 ground truth 값이 제공된 데이터 셋을 활용하여 카메라 외부 파라미터 추정 결과를 확인한다.

• Investigative Magnetic Resonance Imaging
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• 제16권1호
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• pp.55-66
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• 2012

목적 : 자기공명영상(MRI) 획득시 피험자의 머리 움직임은 영상의 품질에 영향을 줄 수 있다. 영상 왜곡의 발생 원인이 되는 피험자의 움직임을 감지하기 위한 3차원 광학 추적 시스템을 제작하였다. 대상 및 방법 : 시스템은 두 대의 CCD 카메라 및 적외선 조명, 구형 반사 마커, 프레임 그래버(frame grabber)와 데스크탑 컴퓨터로 구성되었다. 두 대의 카메라를 이용하여 마커의 움직임을 관측하는 스테레오 비전 시스템을 제작하고, 카메라의 내부/외부 매개변수를 측정하는 캘리브레이션(calibration)과 측정된 매개변수를 이용하여 3차원 움직임 정보를 계산하는 삼각측량(triangulation)기법을 적용하였다. 캘리브레이션 보드와 피험자용 안경을 제작하여 움직임 추적의 정확도와 실제 MRI 영상 촬영 동안의 움직임 검출의 유효성을 평가하였다. 결과 : 반사 마커가 부착된 안경을 쓴 피험자들이 MRI 영상 촬영 동안 머리를 규칙적으로 움직였을 때, 시스템은 MRI의 고자장 환경 내에서도 영상에 영향을 주지 않고 피험자들의 움직임을 잘 감지했다. 결론 : 제작한 스테레오 비전 시스템은피험자의 머리 움직임을 잘 감지하였고, 실시간 알림 기능을 통해 피험자의 움직임을 중지할 수 있도록 알려줌으로써 MRI 영상에 영향을 주는 것을 최소화할 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권4호
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• pp.430-436
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• 2010

The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

• Tuberculosis and Respiratory Diseases
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• 제71권2호
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• pp.88-96
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• 2011

Background: It is known that cigarette smoke (CS) causes cell death. Apoptotic cell death is involved in the pathogenesis of CS-related lung diseases. Some members of the protein kinase C (PKC) family have roles in cigarette smoke extract (CSE)-induced apoptosis. This study was conducted to investigate the role of PKC epsilon in CSE-induced apoptosis in human lung fibroblast cell line, MRC-5. Methods: Lactate dehydrogenase release was measured using a cytotoxicity detection kit. The MTT assay was used to measure cell viability. Western immunoblot, Hoechst 33342 staining and flow cytometry were used to demonstrate the effect of $PKC{\varepsilon}$. Caspase-3 and caspase-8 activities were determined using a colorimetric assay. To examine $PKC{\varepsilon}$ activation, Western blotting was performed using both fractions of membrane and cytosol. Results: We showed that CSE activated $PKC{\varepsilon}$ by demonstrating increased expression of $PKC{\varepsilon}$ in the plasma membrane fraction. Pre-treatment of $PKC{\varepsilon}$ peptide inhibitor attenuated CSE-induced apoptotic cell death, as demonstrated by the MTT assay (13.03% of control, 85.66% of CSE-treatment, and 53.73% of $PKC{\varepsilon}$ peptide inhibitor-pre-treatment, respectively), Hoechst 33342 staining, and flow cytometry (85.64% of CSE-treatment, 53.73% of $PKC{\varepsilon}$ peptide inhibitor-pre-treatment). Pre-treatment of $PKC{\varepsilon}$ peptide inhibitor reduced caspase-3 expression and attenuated caspase-3, caspase-8 activity compared with CSE treatment alone. Conclusion: $PKC{\varepsilon}$ seem to have pro-apoptotic function and exerts its function through the extrinsic apoptotic pathway in CSE-exposed MRC-5 cells. This study suggests that $PKC{\varepsilon}$ inhibition may be a therapeutic strategy in CS-related lung disease such as chronic obstructive pulmonary disease.