• Title/Summary/Keyword: extremely halophilic bacteria, Hazonrcular sp., archaebacteria, amylase

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Amylase Production from Haloarcular sp. EH-1 (고호염성 Haloarcular sp. EH-1으로 부터 amylase 생산)

  • 정명주
    • Journal of Life Science
    • /
    • v.12 no.5
    • /
    • pp.570-576
    • /
    • 2002
  • The extremely halophilic archaebacterium Haloarcular sp. EH-1 was isolated from solar salts. Amylae production from Halonrcular sp. EH-1 have been studied. The results obtained were as follows. The optimal medium composition for the production of amylase from Haloarcular sp. EH-1 were soluble starch 1.5%, yeast extract 1.0%, MgSO$_4$ 7h$_2$O 2.0%, KCI 0.1%, NaCl 25% (pH 7.5). The incubation temperature, aeration rate and agitation speed were 4$0^{\circ}C$, 100 $m\ell$ medium / 500 $m\ell$ shaking flask, and 110 rpm. The cell growth and enzymatic activity was highest at 9 days of incubation. So amylase production appeared to be a growth-related phenomenon.