• Title/Summary/Keyword: extraction temperatures

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Antimicrobial activity of jaborandi extract and sorbitol to oral microbes (Jaborandi extract와 Sorbitol이 구강미생물 증식에 미치는 영향)

  • Jang, Jong-Hwa;You, So-Yeon;Oh, Tae-Jin
    • Journal of Korean society of Dental Hygiene
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    • v.13 no.3
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    • pp.517-523
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    • 2013
  • Objectives : The aim of the study is to investigate the antimicrobial activity of jaborandi and sorbitol to various oral microbes. Methods : Jaborandi leaves contain pilocarpine. The conditions for extraction were optimized on the basis of substances, temperatures and pHs. Total pilocarpine content after extraction was analyzed by HPLC. The herbal antimicrobial activity of jaborandi and sorbitol were evaluated for oral microbes containing ATCC 25175 S. mutans, ATCC 13419 S. salivarius, ATCC 6249 S. mitis, ATCC 33398 S. equi, ATCC 29213 S. aureus, ATCC 14053 C. albicans. Results : The optimum conditions for highest yielding extraction were pilocarpine content after boiling at $100^{\circ}C$ for 1 hour at pH 3. The level of total pilocarpine content was analyzed at 833 mg/kg by HPLC. The most effective antimicrobial activity was obtained by combination of pilocarpine and sorbitol rather than pilocarpine, menthol and sorbitol, respectively. Conclusions : This results supported the preventive oral health care using safe and convenient jaborandi herb.

Changes in the Antioxidant Potential of Persimmon Peel Extracts Prepared by Different Extraction Methods (고종시 감껍질의 추출조건에 따른 항산화 활성)

  • Jeong, Myeong Jin;Jin, Soon Woo;Hwa, Sung Yong;Bang, Hee Ok;Han, Dong Moon;Jeon, Ji Yeong;Hwa, Se Kyung
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.3
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    • pp.186-193
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    • 2019
  • Background: Astringent persimmon (Diospyros kaki Thunb. cv. Kojongsi) peels are by-products of dried persimmons. This study aimed to evaluate the antioxidant activities of Kojongsi persimmon peel (KPP) extracts prepared by 15 different extraction methods: 5 heating durations (0.5 - 2.5 h) at 3 heating temperatures (50, 70, and $90^{\circ}C$). Methods and Results: An increase in heating temperature increased the antioxidant effect of KPP extracts. Those prepared by heating at 1 h had the highest total phenol content, regardless of the heating temperature. In addition, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and cell-protective effects against $H_2O_2-induced$ oxidative stress were dependent on the total phenol contents of the extract. However, the KPP-induced increased in catalase expression was dependent on heating temperature and duration. Conclusions: These results suggest that extraction by heating at $90^{\circ}C$ for 1 h may enhance KPP's antioxidant effects, which mainly involve non-enzymatic antioxidant systems.

Energy Performance Comparison of Electric Heater and Geothermal Source Heat Pump type Agricultural Hot Air Dryers (전기히터식 및 지열원 히트펌프식 농산물 열풍건조기의 에너지 성능 비교)

  • Yang, Won Suk;Kim, Young Il;Park, Seung Tae
    • Journal of the Korean Society for Geothermal and Hydrothermal Energy
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    • v.14 no.4
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    • pp.7-12
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    • 2018
  • Energy performance of electric heater and geothermal source heat pump type hot air dryers are compared in this study. For set temperatures of $30^{\circ}C$, $35^{\circ}C$ and $40^{\circ}C$, radish is dried from initial mass 60 kg until it gets 5 kg, where the difference equals the amount of water removed. As set temperature is increased, drying time is shortened for both electric heater and heat pump types, however energy efficiency is decreased due to increasing electricity consumption. Moisture extraction rate(MER) of electric heater is 2.58~2.84 kg/h, and for heat pump type 2.56~2.71 kg/h, showing little difference between the two types. Specific moisture extraction rate (SMER) of electric heater is 0.94~0.96 kg/kWh, and for heat pump type 1.72~2.21 kg/kWh. SMER of heat pump type is greater by 0.78~1.25 kg/kWh than the electric heater hot air dryer, which is 1.8~2.3 times better in terms of energy efficiency.

Extraction of${\beta}-carotene$ from Carrot by Supercritical Carbon Dioxide (초임계이산화탄소에 의한 당근 중의 ${\beta}-carotene$ 추출)

  • Lim, Sang-Bin;Jwa, Mi-Kyung
    • Korean Journal of Food Science and Technology
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    • v.27 no.3
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    • pp.414-419
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    • 1995
  • ${\beta}-carotene$ was extracted from freeze-dried carrot by supercritical carbon dioxide $(SC-CO_2)$ and mixtures of $CO_2$ doped with ethanol or methanol as a cosolvent at temperatures of 40 to $60^{\circ}C$ and pressures of 138 to 276 bar. Solubility of ${\beta}-carotene$ in $SC-CO_2$ increased with the increase of extraction pressure and the decrease of extraction temperature. The highest solubility observed was $4.90\;{\mu}g/g\;CO_2\;for\;{\alpha}-carotene\;and\;0.604\;{\mu}g/g\;CO_2$ for ${\alpha}-carotene\;at\;40^{\circ}C$ and 276 bar. Addition of ethanol increased the solubility being the largest increase of 82% using a mixture of $CO_2$ and 17.4% ethanol. $SC-CO_2$ extraction can be used to selectively obtain natural carotenoids, free of solvent residuals, which can be used as food additives.

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Quality Properties of Oak Mushroom (Lentinus edodes) Based on Extraction Conditions and Enzyme Treatment (표고버섯(Lentinus edodes)의 추출조건 및 효소처리에 따른 품질 특성)

  • Park, Nan-Young;Jeong, Yong-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.9
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    • pp.1273-1279
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    • 2006
  • The quality properties of oak mushroom were investigated using extraction conditions and enzyme treatments. The physiochemical properties were excellent at the extraction temperatures of $20^{\circ}C\;and\;40^{\circ}C$. The quality was increased as the extraction time increased but was best at the extraction time of 10 hr. The physiochemical properties such as soluble solid, reducing sugar and crude protein contents were best at the solvent ratio of 1:100 (w/v) so that it was set at 1:100. Thus, enzyme treatment was done at $50^{\circ}C$ for 2 hr with the solvent ratio of 1:100. The result showed that the best quality was shown using 0.2% protease and 0.2% cellulase. With the enzyme treatment, the essential amino acid contents increased by two folds but no difference was shown in $\beta-glucan$ content.

Antioxidant and Antigenotoxic Effects of Sansuyu Fruit (Corni fructus) Extracted with Water at Different Temperatures (추출 온도에 따른 산수유의 항산화 활성 및 항유전독성 효과 비교)

  • Lee, Min-Hee;Kim, Jung-Mi;Park, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.149-155
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    • 2011
  • The objective of this study was to evaluate the antioxidant and antigenotoxic activities of sansuyu fruit (Corni fructus, CF) at temperatures of $25^{\circ}C$, $50^{\circ}C$, and $90^{\circ}C$ using a water extraction method. Total phenolic content (TPC), DPPH radical-scavenging activity (RSA), and superoxide dismutase (SOD)-like activity, and ORAC (Oxygen radical absorbance capacity) values were determined. Also the antigenotoxicity of CF was determined by measuring inhibitory effects of $H_2O_2$ induced DNA damage in human leukocytes using the comet assay. The TPC in the CF extracts was 4.2, 4.6, and 5.5 g/100 g GAE in $25^{\circ}C$, $50^{\circ}C$, and $90^{\circ}C$, respectively. The DPPH RSA of the CF extracts increased in a dose-dependent manner over the range of $50\sim1000\;{\mu}g$/mL in all temperatures and the $SC_{50}$ of DPPH RSA of the CF extracts were not significantly different at different extraction temperatures. The $SC_{50}$ of SOD-like was the highest in CF extracted at $25^{\circ}C$ (1.1 mg/mL) followed by $90^{\circ}C$ (1.2 mg/mL) and $50^{\circ}C$ (1.3 mg/mL). The ORAC values of the CF extracts were not significantly different in low concentration ($10\;{\mu}M$/mL) and was in order of $25^{\circ}C$ ($5.7\;{\mu}M$ TE)< $90^{\circ}C$ ($6.2\;{\mu}M$ TE)< $50^{\circ}C$ ($8.5\;{\mu}M$ TE) in high concentration ($50\;{\mu}M$/mL). $200\;{\mu}M$ $H_2O_2$ induced DNA damages in human leukocytes were significantly reduced by the pretreatment with the CF extracts. These results suggest that sansuyu fruit (Corni fructus) can be used as a natural source for antioxidant activities and as antigenotoxic agents regardless of the water extraction temperature.

Optimization of Extraction Conditions for Bakkenolide B from the Leaves of Petasites japonicus by Using Response Surface Methodology (반응표면분석법에 의한 머위(Petasites japonicus)의 bakkenolide B추출공정 최적화)

  • Kim, Jung-Hun;Kim, Yong-Min;Chung, Hun-Sik;Choi, Young-Whan;Lee, Kyoung-Pil;Im, Dong-Soon;Lee, Young-Guen
    • Journal of Life Science
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    • v.24 no.2
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    • pp.167-172
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    • 2014
  • Optimal conditions for extraction of bakkenolide B from Petasites japonicus leaves were determined by using response surface methodology. A second-order Box-Behnken design representing three extraction temperatures (80, 100, $120^{\circ}C$), three extraction times (30, 45, 60 min), and three solvent pH's (5, 7, 9) was executed. The efficiency of the extraction conditions was defined using the ${\beta}$-hexosamidase assay by comparing both the bakkenolide B content and its anti-allergic activity expressed as extract inhibition on degranulation. The response surface plot described for the bakkenolide B content showed that the maximum content was predicted as 121.6 ${\mu}g/g$ with extraction conditions of $127.1^{\circ}C$, 46.6 min, and pH 7.7. Extraction temperature and time were important factors in determining bakkenolide B content. Using regression analysis, correlation between the inhibition effect of mast cell degranulation and bakkenolide B content was found to be low.

Extraction Efficiencies of Organophosphorus Pesticides Spiked in Fish Tissues by Supercritical Carbon Dioxide (초임계 이산화탄소에 의한 어류조직 중 유기인계 농약의 첨가회수율)

  • Lim, Sang-Bin;Jwa, Mi-Kyung
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1163-1168
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    • 1998
  • Fish tissues were spiked with organophosphorus pesticides (OPPs), mixed with a celite as a drying agent, and dynamically extracted with pure $CO_2$ or modified $CO_2$ for 10 min at different extraction temperatures, pressures and $CO_2$ flow rates. Recoveries of OPPs spiked in jacopever increased with the decrease of extraction temperature and pressure, and decreased with the increase of $CO_2$ flow rates. Modified $CO_2$ extractions with 10% methylene chloride showed a slight increase in the recoveries over pure $CO_2$ extraction. Quantity of fish tissues had great effect on their extraction efficiencies. Recoveries of OPPs were $66.7{\sim}86.3%$ for jacopever, $56.2{\sim}79.2%$ for yellow tail, $57.6{\sim}77.8%$ for blanquillo, $84.2{\sim}96.3%$ for sardine, $74.6{\sim}83.6%$ for mackerel. Application of supercritical carbon dioxide extraction offers an attractive alternative to the use of organic solvents for extraction of pesticide residues from fish tissues.

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Quality Comparison of Gelatins Manufactured from Raw and Scalded Pigskins (생박 및 탕박 돈피에서 생산된 젤라틴 품질비교)

  • Lee, Moo-Ha;Kim, Yang-Ha;Chung, Myung-Sub
    • Korean Journal of Food Science and Technology
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    • v.19 no.2
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    • pp.102-106
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    • 1987
  • In order to examine the appropriateness of types of pigskin as a raw material for gelatin production, comparison was made on the quality of gelatins made from raw and scalded pigskins. Raw and scalded pigskins were acidified in 1.7% HCl solution for 15-18 hr and then washed by tap water for 10 hr. After washing, pigskins were extracted at $60^{\circ}C$, $70^{\circ}C$ and $80^{\circ}C$ to produce gelatins. Gelatins from raw pigskins appeared to be better in gel strength than those from scalded ones at all extraction temperatures. Gelatin yield was higher with raw than with scalded pigskins. With the increase of extraction temperature, the decrease in gel strength and viscosity was resulted in. More colored gelatins were produced with increasing extraction temperature in both raw materials. Electrophoretic pattern of gelatins showed that higher molecular weight fractions decreased with the increase of extraction temperature and pigskin gelatin had more complicated molecular composition than that of type B gelatin (alkali-treated gelatin).

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A Study on Extraction Condition of Co-PET from PET/Co-PET Sea-Island Type Microfiber Fabric (PET/Co-PET해도사 직물의 Co-PET추출 조건에 관한 연구)

  • 박명수;윤종호;조대현
    • Textile Coloration and Finishing
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    • v.13 no.2
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    • pp.120-127
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    • 2001
  • In order to make a microfiber fabric with PET/Co-PET Sea-Island Type microfiber, the optimum condition of extraction and elimination of Co-PET from the mocrofiber was examined. At the same time, the physical property change of the fabric with respect to the change of the relative amount of the Co-PET in the microfiber was also examined to provide a directly applicable data set to the industry. The sample fabric used was warp 75/36(DTY) and weft 0.05d(PET/Co-PET, Sea Island Type Microfiber) twill fabric of 36 separated yarns+40/24(high shrinking yarn) with 130/48 ITY. The data set was made at various NaOH concentrations and steam temperatures with time as a main variable. The physical properties examined were the tensile properties. The results obtained were the tensile. The results obtained were 1. For a proper extraction of Co-PET (13.5%)from the microfiber with wet curing, it takes more than 5 min. in 8 and 12% of NaOH solutions but it takes only 3 min. in 18% of NaOH solution at 12$0^{\circ}C$. 2. For a proper extraction of Co-PET (13.5%) from the microfiber with wet curing, ti takes 3~5min. in 12 and 14% of NaOH solution and it takes less than 3 min. in 18% of NaOH solution at $130^\circ{C}$. 3. The increasing ratio of WT increased with increasing NaOH concentrations and the equilibrium point reached was 3 min. at $120^\circ{C}$. 4. The WT increasing ratio was greater in 14 and 18% NaOH solutions than in 8 and 12% of NaOH solutions at $130^\circ{C}$5. The RT ratio changes at $120^\circ{C}$ in 8 and 12% of NaOH solutions were indifferent from that at $130^\circ{C}$ in 12% of NaOH solution. However, the RT was apparently decreased with increasing NaOH concentration.

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